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The map label for this gene is rfbX [H]

Identifier: 150008051

GI number: 150008051

Start: 1641634

End: 1642884

Strand: Reverse

Name: rfbX [H]

Synonym: BDI_1414

Alternate gene names: 150008051

Gene position: 1642884-1641634 (Counterclockwise)

Preceding gene: 150008052

Following gene: 150008050

Centisome position: 34.15

GC content: 28.38

Gene sequence:

>1251_bases
ATGATTTCTTTCTTAAAACTGGAAAATAATAAAACAGCAAAAACGTTATTTGCCAATTTTACTTATTTGTCAATTCTAGA
AATCATAGGATTATTACTTCCCTTGATCTCATATCCATACGTTATAAGGACTGTTGGAGCCGATAACTATGGGGTAGTTG
TTTTTTGTCAAGCTATTATTGCGTATGTAGTTATTATAATAAATTTTGGTTATAACGTTTCTGCAACAAGGAAAATAAGT
GAAAATAGAACTAATGTGTTTAAAATTAGAGAAATATATTCAAGTATTGTATATCAAAAGCTATTGATATTTGCGATATG
CTTGGTGAGTGGGTTGTTCGTATTAATATTCTTGAAATATGATTACTCTGTTATTCTCCTTGGATTTATAGGACTTTGCA
TTCAAGAAGTGTTTTTTCCTACATGGTTGTTTCAAGGACTAGAAAGGATGAAGTTTATAACAATTATCACTTTTGTGGCA
AAGTGTTCATGTCTAATTTTAATTTTTTTGTTCGTACATGATAAAAAAGACTATGCTTGTATTCCAGTATTATATTCAAT
AGGAGGTTTTTTTACATCAGTATTATCGGTTATTATCCTTAAAAAAAAGTTCGATATATATTTTGTGAAGGTGTCTAAGT
ATAGGATGAAAGAGGATTTTTTAGAAAGTCTTCCTTTTTTTACATCTCGTTTATCTGCTATTGTCATGGAAAGAGGTAAT
GTCTTAGTGATAGGAACTTTTTTTTCTTATGATATGGTTGCTATATACGATTTGTGTGCTAAGATAGTTTCAATTTTAAA
AACTCCATTTTCATTAGTAGCACAAGTTATATATCCAAATGTAGCTAAGAGTAAAAATATGCTGCTAGTGAAAAAATCTA
TTAAGATAGTGCTGCTTTTTGGTGCTTTTGTTTGTTTGTTTGTTTACTTATTTGCTCCTAATATTATTTTATTATTAAGT
GATACTTCAATGTTAGGGGCCGTTTCGATATTAAAGATTATGGTTTTATATGTTCCAATAGTGGGGATCAGCTATCTATT
TGGTGCTTCTGTTTTAGTTGTTAAAGGGTATTCAAGGGAATATAATTTAAGTGTTGTATATTCTGTCTTGTTATATATAT
TGATGTTATTATCATTTATCAGTTTTTCTAAAGTAAATCTTTATACAATGGCATTGGCTTTTGTTGCACCCGAATTATTT
GTTGCGTTGTATAGGATTTGTATCGTTAAATATAAAAATATATTAAATTGA

Upstream 100 bases:

>100_bases
TAACGAACTTTTGTAATAGAAATTGTTGTTCCACTGATTATTGATATACGGGGAAAATGCAATATCTCCGATTTGGGTAA
ATATACTTAATATCTATAAA

Downstream 100 bases:

>100_bases
TGGCGTATGTTATCTGATAAGCAAAAAAAACATATTTTGGTTAATCGTGATGGTTTGAAGTATTATACTGTTGAAGGGTT
GGAAAAAGTACACGAAGAAA

Product: putative O-antigen transporter

Products: lipopolysaccharide [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MISFLKLENNKTAKTLFANFTYLSILEIIGLLLPLISYPYVIRTVGADNYGVVVFCQAIIAYVVIIINFGYNVSATRKIS
ENRTNVFKIREIYSSIVYQKLLIFAICLVSGLFVLIFLKYDYSVILLGFIGLCIQEVFFPTWLFQGLERMKFITIITFVA
KCSCLILIFLFVHDKKDYACIPVLYSIGGFFTSVLSVIILKKKFDIYFVKVSKYRMKEDFLESLPFFTSRLSAIVMERGN
VLVIGTFFSYDMVAIYDLCAKIVSILKTPFSLVAQVIYPNVAKSKNMLLVKKSIKIVLLFGAFVCLFVYLFAPNIILLLS
DTSMLGAVSILKIMVLYVPIVGISYLFGASVLVVKGYSREYNLSVVYSVLLYILMLLSFISFSKVNLYTMALAFVAPELF
VALYRICIVKYKNILN

Sequences:

>Translated_416_residues
MISFLKLENNKTAKTLFANFTYLSILEIIGLLLPLISYPYVIRTVGADNYGVVVFCQAIIAYVVIIINFGYNVSATRKIS
ENRTNVFKIREIYSSIVYQKLLIFAICLVSGLFVLIFLKYDYSVILLGFIGLCIQEVFFPTWLFQGLERMKFITIITFVA
KCSCLILIFLFVHDKKDYACIPVLYSIGGFFTSVLSVIILKKKFDIYFVKVSKYRMKEDFLESLPFFTSRLSAIVMERGN
VLVIGTFFSYDMVAIYDLCAKIVSILKTPFSLVAQVIYPNVAKSKNMLLVKKSIKIVLLFGAFVCLFVYLFAPNIILLLS
DTSMLGAVSILKIMVLYVPIVGISYLFGASVLVVKGYSREYNLSVVYSVLLYILMLLSFISFSKVNLYTMALAFVAPELF
VALYRICIVKYKNILN
>Mature_416_residues
MISFLKLENNKTAKTLFANFTYLSILEIIGLLLPLISYPYVIRTVGADNYGVVVFCQAIIAYVVIIINFGYNVSATRKIS
ENRTNVFKIREIYSSIVYQKLLIFAICLVSGLFVLIFLKYDYSVILLGFIGLCIQEVFFPTWLFQGLERMKFITIITFVA
KCSCLILIFLFVHDKKDYACIPVLYSIGGFFTSVLSVIILKKKFDIYFVKVSKYRMKEDFLESLPFFTSRLSAIVMERGN
VLVIGTFFSYDMVAIYDLCAKIVSILKTPFSLVAQVIYPNVAKSKNMLLVKKSIKIVLLFGAFVCLFVYLFAPNIILLLS
DTSMLGAVSILKIMVLYVPIVGISYLFGASVLVVKGYSREYNLSVVYSVLLYILMLLSFISFSKVNLYTMALAFVAPELF
VALYRICIVKYKNILN

Specific function: May be involved in the translocation process of the nascent O-polysaccharide molecules and/or its ligation to lipid A core units [H]

COG id: COG2244

COG function: function code R; Membrane protein involved in the export of O-antigen and teichoic acid

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788349, Length=361, Percent_Identity=24.3767313019391, Blast_Score=144, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002797 [H]

Pfam domain/function: PF01943 Polysacc_synt [H]

EC number: NA

Molecular weight: Translated: 47263; Mature: 47263

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISFLKLENNKTAKTLFANFTYLSILEIIGLLLPLISYPYVIRTVGADNYGVVVFCQAII
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHH
AYVVIIINFGYNVSATRKISENRTNVFKIREIYSSIVYQKLLIFAICLVSGLFVLIFLKY
HHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DYSVILLGFIGLCIQEVFFPTWLFQGLERMKFITIITFVAKCSCLILIFLFVHDKKDYAC
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
IPVLYSIGGFFTSVLSVIILKKKFDIYFVKVSKYRMKEDFLESLPFFTSRLSAIVMERGN
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHCCCC
VLVIGTFFSYDMVAIYDLCAKIVSILKTPFSLVAQVIYPNVAKSKNMLLVKKSIKIVLLF
EEEEEEHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHH
GAFVCLFVYLFAPNIILLLSDTSMLGAVSILKIMVLYVPIVGISYLFGASVLVVKGYSRE
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
YNLSVVYSVLLYILMLLSFISFSKVNLYTMALAFVAPELFVALYRICIVKYKNILN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MISFLKLENNKTAKTLFANFTYLSILEIIGLLLPLISYPYVIRTVGADNYGVVVFCQAII
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHH
AYVVIIINFGYNVSATRKISENRTNVFKIREIYSSIVYQKLLIFAICLVSGLFVLIFLKY
HHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DYSVILLGFIGLCIQEVFFPTWLFQGLERMKFITIITFVAKCSCLILIFLFVHDKKDYAC
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
IPVLYSIGGFFTSVLSVIILKKKFDIYFVKVSKYRMKEDFLESLPFFTSRLSAIVMERGN
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHCCCC
VLVIGTFFSYDMVAIYDLCAKIVSILKTPFSLVAQVIYPNVAKSKNMLLVKKSIKIVLLF
EEEEEEHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHH
GAFVCLFVYLFAPNIILLLSDTSMLGAVSILKIMVLYVPIVGISYLFGASVLVVKGYSRE
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
YNLSVVYSVLLYILMLLSFISFSKVNLYTMALAFVAPELFVALYRICIVKYKNILN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lipopolysaccharide [Cytoplasm] [C]

Specific reaction: lipopolysaccharide [Cytoplasm] = lipopolysaccharide [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7517390; 7517391; 9097040; 9278503 [H]