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The map label for this gene is thiG

Identifier: 150006038

GI number: 150006038

Start: 4395715

End: 4396488

Strand: Direct

Name: thiG

Synonym: BVU_3542

Alternate gene names: 150006038

Gene position: 4395715-4396488 (Clockwise)

Preceding gene: 150006037

Following gene: 150006039

Centisome position: 85.14

GC content: 50.65

Gene sequence:

>774_bases
ATGAAGAAACTGATTATAGCTGGAAAAGAATTTAATTCCCGTTTATTCCTGGGAACCGGTAAATTCAATTCAAACACCGT
AATGGAAGAAGCCATTCTGGCATCAGGGTGTGAAATGGTAACCGTAGCCATGAAACGCATTGAACTGGACGATAAGGAAG
ATGATATGCTGAAACATATCATTCACCCCCACATACAGCTATTGCCCAATACCTCCGGTGTGCGCACAGCCGAAGAAGCC
GTCTTTGCCGCACAAATGGCCCGCGAGGCCTTTGGCACCAACTGGCTGAAACTGGAAATTCATCCCGATCCGCGCTACTT
GTTGCCAGATTCTACCGAAACATTGAAAGCAACCGAGGAACTAGTCAAACTAGGTTTTGTCGTATTGCCCTATTGCCAGG
CAGATCCTACATTATGCAAACGGCTGGAAGAGGCAGGAGCAGCAACCGTCATGCCGCTTGCCGCCCCCATCGGGACAAAC
AAAGGCTTGCAGACACGTGACTTCCTGCGCATCATTATCGAACAAGCTTCCGTTCCTGTGGTAGTAGACGCCGGAATAGG
CGCCCCCAGCCATGCCACCGAAGCGATGGAAATGGGTGCGGACGCCTGTCTGGTCAATACAGCCATCGCAGTAGCCGGCC
AACCGGTAGAAATGGCAATAGCCTTCAAGGAAGCTGTCATTGCCGGACGCCGCGCCTATGAAGCAGGCTTAGGAGCAGTA
GGACAGAACTTGATCGCATCCGCCTCATCCCCTTTAACTTCATTCTTAGATTAA

Upstream 100 bases:

>100_bases
GAATCACCGGTATTGCCTTGAGCGGAACCATACTCCGGGCGAAAGACCCGGTAGCGGAAACTAAACGAATCATGAATTTA
TAATAAAATCACAAAATACA

Downstream 100 bases:

>100_bases
ACAGAAATATGATAGACAAAAAGCAAGATAAAAAAGCATACGCTCATGCAGAAAAGGCATATATGCAAGGAACCCTGTTC
CCATACATCAAAGTAGGAAT

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MKKLIIAGKEFNSRLFLGTGKFNSNTVMEEAILASGCEMVTVAMKRIELDDKEDDMLKHIIHPHIQLLPNTSGVRTAEEA
VFAAQMAREAFGTNWLKLEIHPDPRYLLPDSTETLKATEELVKLGFVVLPYCQADPTLCKRLEEAGAATVMPLAAPIGTN
KGLQTRDFLRIIIEQASVPVVVDAGIGAPSHATEAMEMGADACLVNTAIAVAGQPVEMAIAFKEAVIAGRRAYEAGLGAV
GQNLIASASSPLTSFLD

Sequences:

>Translated_257_residues
MKKLIIAGKEFNSRLFLGTGKFNSNTVMEEAILASGCEMVTVAMKRIELDDKEDDMLKHIIHPHIQLLPNTSGVRTAEEA
VFAAQMAREAFGTNWLKLEIHPDPRYLLPDSTETLKATEELVKLGFVVLPYCQADPTLCKRLEEAGAATVMPLAAPIGTN
KGLQTRDFLRIIIEQASVPVVVDAGIGAPSHATEAMEMGADACLVNTAIAVAGQPVEMAIAFKEAVIAGRRAYEAGLGAV
GQNLIASASSPLTSFLD
>Mature_257_residues
MKKLIIAGKEFNSRLFLGTGKFNSNTVMEEAILASGCEMVTVAMKRIELDDKEDDMLKHIIHPHIQLLPNTSGVRTAEEA
VFAAQMAREAFGTNWLKLEIHPDPRYLLPDSTETLKATEELVKLGFVVLPYCQADPTLCKRLEEAGAATVMPLAAPIGTN
KGLQTRDFLRIIIEQASVPVVVDAGIGAPSHATEAMEMGADACLVNTAIAVAGQPVEMAIAFKEAVIAGRRAYEAGLGAV
GQNLIASASSPLTSFLD

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=254, Percent_Identity=64.9606299212598, Blast_Score=325, Evalue=2e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_BACV8 (A6L646)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001300782.1
- ProteinModelPortal:   A6L646
- SMR:   A6L646
- STRING:   A6L646
- GeneID:   5304502
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_3542
- NMPDR:   fig|435590.6.peg.3294
- eggNOG:   COG2022
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- ProtClustDB:   PRK00208
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27518; Mature: 27518

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: ACT_SITE 97-97 BINDING 158-158

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLIIAGKEFNSRLFLGTGKFNSNTVMEEAILASGCEMVTVAMKRIELDDKEDDMLKHI
CCCEEEECCCCCCEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHH
IHPHIQLLPNTSGVRTAEEAVFAAQMAREAFGTNWLKLEIHPDPRYLLPDSTETLKATEE
HCCHHEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEECCCCCHHHHHHHH
LVKLGFVVLPYCQADPTLCKRLEEAGAATVMPLAAPIGTNKGLQTRDFLRIIIEQASVPV
HHHHHHHEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCE
VVDAGIGAPSHATEAMEMGADACLVNTAIAVAGQPVEMAIAFKEAVIAGRRAYEAGLGAV
EEECCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GQNLIASASSPLTSFLD
HHHHHHCCCCCHHHHCC
>Mature Secondary Structure
MKKLIIAGKEFNSRLFLGTGKFNSNTVMEEAILASGCEMVTVAMKRIELDDKEDDMLKHI
CCCEEEECCCCCCEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHH
IHPHIQLLPNTSGVRTAEEAVFAAQMAREAFGTNWLKLEIHPDPRYLLPDSTETLKATEE
HCCHHEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEECCCCCHHHHHHHH
LVKLGFVVLPYCQADPTLCKRLEEAGAATVMPLAAPIGTNKGLQTRDFLRIIIEQASVPV
HHHHHHHEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCE
VVDAGIGAPSHATEAMEMGADACLVNTAIAVAGQPVEMAIAFKEAVIAGRRAYEAGLGAV
EEECCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GQNLIASASSPLTSFLD
HHHHHHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA