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The map label for this gene is thiE

Identifier: 150006037

GI number: 150006037

Start: 4395071

End: 4395697

Strand: Direct

Name: thiE

Synonym: BVU_3541

Alternate gene names: 150006037

Gene position: 4395071-4395697 (Clockwise)

Preceding gene: 150006036

Following gene: 150006038

Centisome position: 85.12

GC content: 47.37

Gene sequence:

>627_bases
GTGGAGGATAAGACCGTGGAACTTCAATTCATCACTCACTTCACCGACACATATTCCTATTATGATTCCGCACGCATGGC
CTTGGAAGGCGGTTGCCGCTGGATCCAGCTACGCATGAAAGACACTCCCGTTGACGAAGTGGAACGGGAAGCCATACGCC
TGCAAGGCTTATGCAAAGACTATGGAGCGACATTTGTCATAGACGACCACGTGGAGTTAGTTAAGAAAATTCATGCTGAC
GGAGTGCATTTAGGAAAAAAGGACATGCCCGTAGCCGAAGCACGGGGAATATTAGGAAAAGAATTCATCATCGGAGGCAC
CGCCAATACATTTGACGATGTAAAGATGCATTACAAAGCCGGAGCTGACTACATCGGCTGTGGCCCGTTCCGTTTTACTA
CTACAAAAAAGGATCTCTCTCCAGTGCTGGGACTGGAAGGTTACCGTTCCATTATTCTGCAAATGAAAGAGGCAAATATC
CATTTGCCCATCGTTGCTATCGGGGGAATTACTTTGGAAGATATCCCTTCCATCATGGAAACGGGAATCACCGGTATTGC
CTTGAGCGGAACCATACTCCGGGCGAAAGACCCGGTAGCGGAAACTAAACGAATCATGAATTTATAA

Upstream 100 bases:

>100_bases
GGTATCGCCATAGCGGTAAACAACCGGATGGTTCCCCGTATAGAGTGGGAGAAATGTGTGTTACACCCCAATGACAGTCT
GGTAGTCATTAAAGCTGCAT

Downstream 100 bases:

>100_bases
TAAAATCACAAAATACAATGAAGAAACTGATTATAGCTGGAAAAGAATTTAATTCCCGTTTATTCCTGGGAACCGGTAAA
TTCAATTCAAACACCGTAAT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MEDKTVELQFITHFTDTYSYYDSARMALEGGCRWIQLRMKDTPVDEVEREAIRLQGLCKDYGATFVIDDHVELVKKIHAD
GVHLGKKDMPVAEARGILGKEFIIGGTANTFDDVKMHYKAGADYIGCGPFRFTTTKKDLSPVLGLEGYRSIILQMKEANI
HLPIVAIGGITLEDIPSIMETGITGIALSGTILRAKDPVAETKRIMNL

Sequences:

>Translated_208_residues
MEDKTVELQFITHFTDTYSYYDSARMALEGGCRWIQLRMKDTPVDEVEREAIRLQGLCKDYGATFVIDDHVELVKKIHAD
GVHLGKKDMPVAEARGILGKEFIIGGTANTFDDVKMHYKAGADYIGCGPFRFTTTKKDLSPVLGLEGYRSIILQMKEANI
HLPIVAIGGITLEDIPSIMETGITGIALSGTILRAKDPVAETKRIMNL
>Mature_208_residues
MEDKTVELQFITHFTDTYSYYDSARMALEGGCRWIQLRMKDTPVDEVEREAIRLQGLCKDYGATFVIDDHVELVKKIHAD
GVHLGKKDMPVAEARGILGKEFIIGGTANTFDDVKMHYKAGADYIGCGPFRFTTTKKDLSPVLGLEGYRSIILQMKEANI
HLPIVAIGGITLEDIPSIMETGITGIALSGTILRAKDPVAETKRIMNL

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=183, Percent_Identity=31.6939890710383, Blast_Score=82, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6325042, Length=195, Percent_Identity=32.3076923076923, Blast_Score=92, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_BACV8 (A6L645)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001300781.1
- ProteinModelPortal:   A6L645
- STRING:   A6L645
- GeneID:   5304501
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_3541
- NMPDR:   fig|435590.6.peg.3293
- eggNOG:   COG0352
- HOGENOM:   HBG754477
- OMA:   RTCATIC
- ProtClustDB:   PRK00043
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 23064; Mature: 23064

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDKTVELQFITHFTDTYSYYDSARMALEGGCRWIQLRMKDTPVDEVEREAIRLQGLCKD
CCCCEEEEEEEEEHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
YGATFVIDDHVELVKKIHADGVHLGKKDMPVAEARGILGKEFIIGGTANTFDDVKMHYKA
HCCEEEEEHHHHHHHHHHCCCEECCCCCCCHHHHCCCCCCEEEECCCCCCHHHHHHHHHC
GADYIGCGPFRFTTTKKDLSPVLGLEGYRSIILQMKEANIHLPIVAIGGITLEDIPSIME
CCCEECCCCEEEECCHHHHHHHHCCCHHHHHHEEHCCCCEEEEEEEECCCCHHHHHHHHH
TGITGIALSGTILRAKDPVAETKRIMNL
CCCCEEEECCEEEECCCCHHHHHHHHCC
>Mature Secondary Structure
MEDKTVELQFITHFTDTYSYYDSARMALEGGCRWIQLRMKDTPVDEVEREAIRLQGLCKD
CCCCEEEEEEEEEHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
YGATFVIDDHVELVKKIHADGVHLGKKDMPVAEARGILGKEFIIGGTANTFDDVKMHYKA
HCCEEEEEHHHHHHHHHHCCCEECCCCCCCHHHHCCCCCCEEEECCCCCCHHHHHHHHHC
GADYIGCGPFRFTTTKKDLSPVLGLEGYRSIILQMKEANIHLPIVAIGGITLEDIPSIME
CCCEECCCCEEEECCHHHHHHHHCCCHHHHHHEEHCCCCEEEEEEEECCCCHHHHHHHHH
TGITGIALSGTILRAKDPVAETKRIMNL
CCCCEEEECCEEEECCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA