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The map label for this gene is thiC [H]

Identifier: 150006039

GI number: 150006039

Start: 4396497

End: 4398197

Strand: Direct

Name: thiC [H]

Synonym: BVU_3543

Alternate gene names: 150006039

Gene position: 4396497-4398197 (Clockwise)

Preceding gene: 150006038

Following gene: 150006040

Centisome position: 85.15

GC content: 48.97

Gene sequence:

>1701_bases
ATGATAGACAAAAAGCAAGATAAAAAAGCATACGCTCATGCAGAAAAGGCATATATGCAAGGAACCCTGTTCCCATACAT
CAAAGTAGGAATGCAGAAAGTAAACCTGACTCCTACTGTAAACATAGTGAACGGTGAAAAGGTAACAACGCCCAATGCTC
CTGTTTATATTTATGACACCAGTGGTCCGTTCAGTGATCCCAATATGGAAATCGATCTGAAAAAAGGACTTCCCCGTATG
CGCGAAAGCTGGATTATTGGACGGGGAGACGTGGAAAAACTGCCCTCCATTACCTCAGAATACGGAAAGATGCGCCGTGA
CGATAAAAGTCTGGACCACCTGCGCTTTGAACACATCGCCCTGCCCTATCGTGCCAAAGCCGGAAAGGCCATCACACAAA
TGGCATACGCCAAAGCCGGCATAGTTACACCGGAAATGGAATATGTAGCCATCCGTGAAAACATGAATTGCCGGGAACTG
GGCATCGACACCTTTATCACCCCTGAATTCGTGCGTGATGAAATAGCCGCAGGGCGTGCCGTACTTCCCGCCAATATCAA
CCACCCCGAAAGCGAGCCTATGATTATCGGCAGGAACTTTTTAGTGAAGATCAACACGAACATCGGAAACTCCGCCACCA
CTTCCTCCATTGACGAAGAGGTGGAAAAAGCCGTGTGGAGCTGCAAATGGGGAGGTGATACCCTGATGGATCTCTCGACC
GGCGATAACATTCACGAAACACGCGAATGGATTGTCCGCAACTGTCCTGTCCCCGTAGGTACCGTACCTATTTATCAGGC
ATTAGAGAAAGTAAACGGTAAAGTAGAGAATCTGAATTGGGAGATTTATAAAGATACATTAATCGAACAATGCGAACAAG
GAGTGGACTACTTTACCATCCATGCCGGAATACGCCGCCAGAATGTACATCTGGCCGACAAACGTCTGTGTGGCATCGTA
AGCCGCGGAGGCAGCATCATGAGCAAGTGGTGTCTGGTACATGACAAGGAAAGTTTCTTATATGAGCATTTTGATGACAT
CTGTGATATTCTGGCACAATACGATGTAGCCGTATCCTTGGGTGACGGACTTCGTCCGGGCTGTATCGCTGACGCTAATG
ACGAGGCACAGTTTGCCGAACTGGATACCATGGGTGAACTTGTACTCCGAGCATGGAACAAAAACGTACAGGCATTCATT
GAAGGACCGGGGCATGTACCTTTGCATAAAATCAAAGAAAACATGGAACGCCAGATCTCTCACTGTCACAACGCACCGTT
CTACACCCTCGGTCCGCTGGTAACGGACATCGCTCCGGGATATGACCATATCACTTCGGCCATCGGTGCCGCACAAATCG
GCTGGTTAGGCACTGCCATGCTATGTTATGTCACCCCGAAAGAACATCTGGGATTGCCCAACAAAGAAGATGTACGCATA
GGTGTCATCACTTACAAGATTGCTGCCCATGCTGCCGATCTAGCGAAAGGGCATCCGGGCGCACAGATCCGCGACAATGC
TTTAAGCAAGGCACGTTATGAATTCCGTTGGCGCGACCAGTTCCATCTGAGTCTGGATCCCGACCGCGCTTTGGAATACT
TCAATGAAGGCCGCCATACGGACGGTGAGTACTGTACCATGTGCGGACCCAACTTCTGCGCTATGAAACTGAGCCGCGAC
TTGAAGAACGTGGGTAATTAA

Upstream 100 bases:

>100_bases
CCGGACGCCGCGCCTATGAAGCAGGCTTAGGAGCAGTAGGACAGAACTTGATCGCATCCGCCTCATCCCCTTTAACTTCA
TTCTTAGATTAAACAGAAAT

Downstream 100 bases:

>100_bases
ACAACTGACTTTGTAAAAACTATGGAAGATTTCAAGAAAGCACTTGAAGGAACTTTAGGAAGAAAACACATAGACAACAT
AGTAGATCAAGTGGCCAGCT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MIDKKQDKKAYAHAEKAYMQGTLFPYIKVGMQKVNLTPTVNIVNGEKVTTPNAPVYIYDTSGPFSDPNMEIDLKKGLPRM
RESWIIGRGDVEKLPSITSEYGKMRRDDKSLDHLRFEHIALPYRAKAGKAITQMAYAKAGIVTPEMEYVAIRENMNCREL
GIDTFITPEFVRDEIAAGRAVLPANINHPESEPMIIGRNFLVKINTNIGNSATTSSIDEEVEKAVWSCKWGGDTLMDLST
GDNIHETREWIVRNCPVPVGTVPIYQALEKVNGKVENLNWEIYKDTLIEQCEQGVDYFTIHAGIRRQNVHLADKRLCGIV
SRGGSIMSKWCLVHDKESFLYEHFDDICDILAQYDVAVSLGDGLRPGCIADANDEAQFAELDTMGELVLRAWNKNVQAFI
EGPGHVPLHKIKENMERQISHCHNAPFYTLGPLVTDIAPGYDHITSAIGAAQIGWLGTAMLCYVTPKEHLGLPNKEDVRI
GVITYKIAAHAADLAKGHPGAQIRDNALSKARYEFRWRDQFHLSLDPDRALEYFNEGRHTDGEYCTMCGPNFCAMKLSRD
LKNVGN

Sequences:

>Translated_566_residues
MIDKKQDKKAYAHAEKAYMQGTLFPYIKVGMQKVNLTPTVNIVNGEKVTTPNAPVYIYDTSGPFSDPNMEIDLKKGLPRM
RESWIIGRGDVEKLPSITSEYGKMRRDDKSLDHLRFEHIALPYRAKAGKAITQMAYAKAGIVTPEMEYVAIRENMNCREL
GIDTFITPEFVRDEIAAGRAVLPANINHPESEPMIIGRNFLVKINTNIGNSATTSSIDEEVEKAVWSCKWGGDTLMDLST
GDNIHETREWIVRNCPVPVGTVPIYQALEKVNGKVENLNWEIYKDTLIEQCEQGVDYFTIHAGIRRQNVHLADKRLCGIV
SRGGSIMSKWCLVHDKESFLYEHFDDICDILAQYDVAVSLGDGLRPGCIADANDEAQFAELDTMGELVLRAWNKNVQAFI
EGPGHVPLHKIKENMERQISHCHNAPFYTLGPLVTDIAPGYDHITSAIGAAQIGWLGTAMLCYVTPKEHLGLPNKEDVRI
GVITYKIAAHAADLAKGHPGAQIRDNALSKARYEFRWRDQFHLSLDPDRALEYFNEGRHTDGEYCTMCGPNFCAMKLSRD
LKNVGN
>Mature_566_residues
MIDKKQDKKAYAHAEKAYMQGTLFPYIKVGMQKVNLTPTVNIVNGEKVTTPNAPVYIYDTSGPFSDPNMEIDLKKGLPRM
RESWIIGRGDVEKLPSITSEYGKMRRDDKSLDHLRFEHIALPYRAKAGKAITQMAYAKAGIVTPEMEYVAIRENMNCREL
GIDTFITPEFVRDEIAAGRAVLPANINHPESEPMIIGRNFLVKINTNIGNSATTSSIDEEVEKAVWSCKWGGDTLMDLST
GDNIHETREWIVRNCPVPVGTVPIYQALEKVNGKVENLNWEIYKDTLIEQCEQGVDYFTIHAGIRRQNVHLADKRLCGIV
SRGGSIMSKWCLVHDKESFLYEHFDDICDILAQYDVAVSLGDGLRPGCIADANDEAQFAELDTMGELVLRAWNKNVQAFI
EGPGHVPLHKIKENMERQISHCHNAPFYTLGPLVTDIAPGYDHITSAIGAAQIGWLGTAMLCYVTPKEHLGLPNKEDVRI
GVITYKIAAHAADLAKGHPGAQIRDNALSKARYEFRWRDQFHLSLDPDRALEYFNEGRHTDGEYCTMCGPNFCAMKLSRD
LKNVGN

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=577, Percent_Identity=61.1785095320624, Blast_Score=747, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 63473; Mature: 63473

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDKKQDKKAYAHAEKAYMQGTLFPYIKVGMQKVNLTPTVNIVNGEKVTTPNAPVYIYDT
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCEEECCCEEEEEECCCEEECCCCCEEEEEC
SGPFSDPNMEIDLKKGLPRMRESWIIGRGDVEKLPSITSEYGKMRRDDKSLDHLRFEHIA
CCCCCCCCEEEEHHHCCCHHHHCEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHEE
LPYRAKAGKAITQMAYAKAGIVTPEMEYVAIRENMNCRELGIDTFITPEFVRDEIAAGRA
CCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHCCCCCCCHHHHHHHHHCCCE
VLPANINHPESEPMIIGRNFLVKINTNIGNSATTSSIDEEVEKAVWSCKWGGDTLMDLST
EECCCCCCCCCCCEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECC
GDNIHETREWIVRNCPVPVGTVPIYQALEKVNGKVENLNWEIYKDTLIEQCEQGVDYFTI
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCEEECCCEEEHHHHHHHHHHCCCCEEEE
HAGIRRQNVHLADKRLCGIVSRGGSIMSKWCLVHDKESFLYEHFDDICDILAQYDVAVSL
ECCCEECCCCHHHHHHHHHHHCCCHHHHHHHHEECHHHHHHHHHHHHHHHHHHHCEEEEE
GDGLRPGCIADANDEAQFAELDTMGELVLRAWNKNVQAFIEGPGHVPLHKIKENMERQIS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHH
HCHNAPFYTLGPLVTDIAPGYDHITSAIGAAQIGWLGTAMLCYVTPKEHLGLPNKEDVRI
HHCCCCCEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCCCCEEE
GVITYKIAAHAADLAKGHPGAQIRDNALSKARYEFRWRDQFHLSLDPDRALEYFNEGRHT
EEEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHEEEEECCEEEEECCHHHHHHHHHCCCCC
DGEYCTMCGPNFCAMKLSRDLKNVGN
CCCEEEECCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIDKKQDKKAYAHAEKAYMQGTLFPYIKVGMQKVNLTPTVNIVNGEKVTTPNAPVYIYDT
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCEEECCCEEEEEECCCEEECCCCCEEEEEC
SGPFSDPNMEIDLKKGLPRMRESWIIGRGDVEKLPSITSEYGKMRRDDKSLDHLRFEHIA
CCCCCCCCEEEEHHHCCCHHHHCEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHEE
LPYRAKAGKAITQMAYAKAGIVTPEMEYVAIRENMNCRELGIDTFITPEFVRDEIAAGRA
CCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHCCCCCCCHHHHHHHHHCCCE
VLPANINHPESEPMIIGRNFLVKINTNIGNSATTSSIDEEVEKAVWSCKWGGDTLMDLST
EECCCCCCCCCCCEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECC
GDNIHETREWIVRNCPVPVGTVPIYQALEKVNGKVENLNWEIYKDTLIEQCEQGVDYFTI
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCEEECCCEEEHHHHHHHHHHCCCCEEEE
HAGIRRQNVHLADKRLCGIVSRGGSIMSKWCLVHDKESFLYEHFDDICDILAQYDVAVSL
ECCCEECCCCHHHHHHHHHHHCCCHHHHHHHHEECHHHHHHHHHHHHHHHHHHHCEEEEE
GDGLRPGCIADANDEAQFAELDTMGELVLRAWNKNVQAFIEGPGHVPLHKIKENMERQIS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHH
HCHNAPFYTLGPLVTDIAPGYDHITSAIGAAQIGWLGTAMLCYVTPKEHLGLPNKEDVRI
HHCCCCCEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCCCCEEE
GVITYKIAAHAADLAKGHPGAQIRDNALSKARYEFRWRDQFHLSLDPDRALEYFNEGRHT
EEEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHEEEEECCEEEEECCHHHHHHHHHCCCCC
DGEYCTMCGPNFCAMKLSRDLKNVGN
CCCEEEECCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]