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The map label for this gene is rimO [H]

Identifier: 148827825

GI number: 148827825

Start: 1188798

End: 1189454

Strand: Direct

Name: rimO [H]

Synonym: CGSHiGG_06515

Alternate gene names: 148827825

Gene position: 1188798-1189454 (Clockwise)

Preceding gene: 148827819

Following gene: 148827831

Centisome position: 62.99

GC content: 39.88

Gene sequence:

>657_bases
TTGCCCAAAAAACTTGGCATTTGGGTTCGCTTACATTATGTGTATCCCTATCCCCATGTGGATGATTTAATTCCATTAAT
GGCTGACGGCACTCTCTTGCCGTATTTAGACATTCCATTACAACACGCCAGCCCTAAAATCTTAAAAGCAATGAAAAGAC
CAGGCAGTATTGACCGCACTTTAGAACGCATTAAACAATGGCGTGAAATTTGCCCAGATTTAACATTACGATCCACCTTT
ATTGTAGGTTTCCCTGGCGAAACGGAAGAAGATTTCCAATTATTACTCGATTTCTTAAAAGAAGCCCAACTTGATCGCGT
AGGCTGTTTCAAATTCAGCCCTGTTGAAGGTGCACCCGCAACAGAGATGGCTGACCAAGTACCAGAAGATGTGAAAGAAG
AACGCTTCCATCGCTTCATGCAATTACAACAAGAAATTTCTGCAAACCGTTTAAAACAAAAAATTGGTAAAACCCTTGAT
GTATTAGTGGATGAAATTGACGAAGATGGTATTATTGGTCGTTCAAAAGCTGATGCCCCTGAAGTAGATGGGTTAGTTTA
CGTAGATAATTTAAGCGGAATCAATGTCAAAGTCGGTGACGTGATTAAAGTAACCATCACAAATTCTGATGAATATGATT
TGTGGGGCAGTTGTTAA

Upstream 100 bases:

>100_bases
CGAAAATTGAGTAAAATCTTGTGCTTGAAATTTTATAGAGCGAAAAAACGGTTATTTTTATGCAAAATTCAACTCCAAGC
ATTGGCTTTGTAAGCCTTGG

Downstream 100 bases:

>100_bases
GTAACAACAATCTCAACGAAATAAACAAAAAATCCATCTTCGTATTGAAGATGGATTTTTTTATTAACCAAACACTTTTT
CCAAATGTGCTTGGTAGTCT

Product: 2-methylthioadenine synthetase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MPKKLGIWVRLHYVYPYPHVDDLIPLMADGTLLPYLDIPLQHASPKILKAMKRPGSIDRTLERIKQWREICPDLTLRSTF
IVGFPGETEEDFQLLLDFLKEAQLDRVGCFKFSPVEGAPATEMADQVPEDVKEERFHRFMQLQQEISANRLKQKIGKTLD
VLVDEIDEDGIIGRSKADAPEVDGLVYVDNLSGINVKVGDVIKVTITNSDEYDLWGSC

Sequences:

>Translated_218_residues
MPKKLGIWVRLHYVYPYPHVDDLIPLMADGTLLPYLDIPLQHASPKILKAMKRPGSIDRTLERIKQWREICPDLTLRSTF
IVGFPGETEEDFQLLLDFLKEAQLDRVGCFKFSPVEGAPATEMADQVPEDVKEERFHRFMQLQQEISANRLKQKIGKTLD
VLVDEIDEDGIIGRSKADAPEVDGLVYVDNLSGINVKVGDVIKVTITNSDEYDLWGSC
>Mature_217_residues
PKKLGIWVRLHYVYPYPHVDDLIPLMADGTLLPYLDIPLQHASPKILKAMKRPGSIDRTLERIKQWREICPDLTLRSTFI
VGFPGETEEDFQLLLDFLKEAQLDRVGCFKFSPVEGAPATEMADQVPEDVKEERFHRFMQLQQEISANRLKQKIGKTLDV
LVDEIDEDGIIGRSKADAPEVDGLVYVDNLSGINVKVGDVIKVTITNSDEYDLWGSC

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=219, Percent_Identity=27.8538812785388, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI28872784, Length=219, Percent_Identity=27.8538812785388, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI93277076, Length=163, Percent_Identity=30.0613496932515, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1787057, Length=214, Percent_Identity=74.2990654205608, Blast_Score=345, Evalue=1e-96,
Organism=Escherichia coli, GI1786882, Length=204, Percent_Identity=29.9019607843137, Blast_Score=92, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21356207, Length=221, Percent_Identity=28.5067873303167, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19922432, Length=202, Percent_Identity=25.7425742574257, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 24755; Mature: 24624

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKKLGIWVRLHYVYPYPHVDDLIPLMADGTLLPYLDIPLQHASPKILKAMKRPGSIDRT
CCCCEEEEEEEEEEECCCCHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHHHCCCCHHHH
LERIKQWREICPDLTLRSTFIVGFPGETEEDFQLLLDFLKEAQLDRVGCFKFSPVEGAPA
HHHHHHHHHHCCCCEECCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCH
TEMADQVPEDVKEERFHRFMQLQQEISANRLKQKIGKTLDVLVDEIDEDGIIGRSKADAP
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
EVDGLVYVDNLSGINVKVGDVIKVTITNSDEYDLWGSC
CCCCEEEEECCCCCEEEECCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
PKKLGIWVRLHYVYPYPHVDDLIPLMADGTLLPYLDIPLQHASPKILKAMKRPGSIDRT
CCCEEEEEEEEEEECCCCHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHHHCCCCHHHH
LERIKQWREICPDLTLRSTFIVGFPGETEEDFQLLLDFLKEAQLDRVGCFKFSPVEGAPA
HHHHHHHHHHCCCCEECCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCH
TEMADQVPEDVKEERFHRFMQLQQEISANRLKQKIGKTLDVLVDEIDEDGIIGRSKADAP
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
EVDGLVYVDNLSGINVKVGDVIKVTITNSDEYDLWGSC
CCCCEEEEECCCCCEEEECCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA