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The map label for this gene is prfB [H]

Identifier: 148824297

GI number: 148824297

Start: 3484532

End: 3485668

Strand: Reverse

Name: prfB [H]

Synonym: TBFG_13122

Alternate gene names: 148824297

Gene position: 3485668-3484532 (Counterclockwise)

Preceding gene: 148824299

Following gene: 148824296

Centisome position: 78.78

GC content: 65.35

Gene sequence:

>1137_bases
ATGCCGGTAACCTTGGCTGCTGTGGACCCCGACCGTCAAGCCGACATCGCCGCCTTGGACTGCACCCTGACCACGGTGGA
GCGGGTGCTCGACGTCGAGGGTCTGCGCAGCCGCATCGAGAAGCTCGAGCATGAGGCATCTGATCCGCACTTGTGGGACG
ACCAGACCCGGGCGCAGCGGGTGACCAGCGAGTTGTCGCACACCCAGGGAGAGCTGCGGCGCGTCGAGGAGCTAAGGCGC
CGCCTGGACGACCTGCCGGTGCTCTACGAGCTGGCCGCCGAGGAAGCGGGCGCGGCCGCCGCCGATGCCGTCGCCGAGGC
CGACGCGGAGCTCAAGTCGTTGCGCGCCGACATCGAGGCCACCGAGGTGCGCACCCTGCTGTCGGGGGAATACGACGAGC
GTGAGGCGCTGGTCACCATCCGGTCCGGCGCGGGTGGGGTGGACGCCGCAGACTGGGCCGAGATGCTGATGCGGATGTAT
ATCCGCTGGGCCGAGCAGCACAAATACCCCGTCGAGGTGTTCGACACCTCCTATGCCGAAGAAGCCGGCATCAAAAGCGC
CACGTTCGCCGTGCACGCACCGTTCGCCTACGGCACGTTGTCGGTCGAACAGGGCACCCACCGGCTGGTGCGGATCAGCC
CGTTCGACAACCAGAGCCGGCGACAGACGTCGTTCGCCGAAGTCGAAGTGCTGCCGGTGGTGGAGACCACCGACCACATT
GACATCCCCGAGGGCGATGTCCGCGTCGACGTCTATCGCTCCAGCGGGCCCGGCGGGCAATCGGTGAACACGACAGACTC
AGCGGTGCGGCTCACCCACATTCCCAGCGGTATCGTGGTCACCTGCCAGAACGAGAAGTCCCAACTGCAGAACAAGATCG
CGGCGATGCGGGTTCTGCAAGCAAAGTTGTTGGAACGCAAGCGCTTAGAAGAACGCGCCGAGCTCGACGCGTTGAAGGCC
GACGGCGGCAGCTCCTGGGGTAACCAGATGCGTTCCTATGTGCTGCACCCATATCAGATGGTCAAGGATCTGCGCACCGA
GTACGAGGTCGGCAATCCGGCGGCCGTTCTGGATGGAGACCTCGACGGATTCCTGGAAGCCGGGATCCGGTGGCGCAATC
GACGTAATGACGACTAG

Upstream 100 bases:

>100_bases
CGAGCCTTCTGTGTTGTGCCCGGCTGCGGCGCAAGGGGCTGCGAATGGGCTGGGCCGCAGTCGTGCGGTGCACATGACGG
TCATCTGATGCTAGACGCTG

Downstream 100 bases:

>100_bases
CGGCACAGTTCTGGCCACCTCGATAGCACAGCACTGGCATAACTTCTGGCGCGGTGAAATCGGCGACTGGATCCTCAACC
GGGGCCTGCGCATCGTCATG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MPVTLAAVDPDRQADIAALDCTLTTVERVLDVEGLRSRIEKLEHEASDPHLWDDQTRAQRVTSELSHTQGELRRVEELRR
RLDDLPVLYELAAEEAGAAAADAVAEADAELKSLRADIEATEVRTLLSGEYDEREALVTIRSGAGGVDAADWAEMLMRMY
IRWAEQHKYPVEVFDTSYAEEAGIKSATFAVHAPFAYGTLSVEQGTHRLVRISPFDNQSRRQTSFAEVEVLPVVETTDHI
DIPEGDVRVDVYRSSGPGGQSVNTTDSAVRLTHIPSGIVVTCQNEKSQLQNKIAAMRVLQAKLLERKRLEERAELDALKA
DGGSSWGNQMRSYVLHPYQMVKDLRTEYEVGNPAAVLDGDLDGFLEAGIRWRNRRNDD

Sequences:

>Translated_378_residues
MPVTLAAVDPDRQADIAALDCTLTTVERVLDVEGLRSRIEKLEHEASDPHLWDDQTRAQRVTSELSHTQGELRRVEELRR
RLDDLPVLYELAAEEAGAAAADAVAEADAELKSLRADIEATEVRTLLSGEYDEREALVTIRSGAGGVDAADWAEMLMRMY
IRWAEQHKYPVEVFDTSYAEEAGIKSATFAVHAPFAYGTLSVEQGTHRLVRISPFDNQSRRQTSFAEVEVLPVVETTDHI
DIPEGDVRVDVYRSSGPGGQSVNTTDSAVRLTHIPSGIVVTCQNEKSQLQNKIAAMRVLQAKLLERKRLEERAELDALKA
DGGSSWGNQMRSYVLHPYQMVKDLRTEYEVGNPAAVLDGDLDGFLEAGIRWRNRRNDD
>Mature_377_residues
PVTLAAVDPDRQADIAALDCTLTTVERVLDVEGLRSRIEKLEHEASDPHLWDDQTRAQRVTSELSHTQGELRRVEELRRR
LDDLPVLYELAAEEAGAAAADAVAEADAELKSLRADIEATEVRTLLSGEYDEREALVTIRSGAGGVDAADWAEMLMRMYI
RWAEQHKYPVEVFDTSYAEEAGIKSATFAVHAPFAYGTLSVEQGTHRLVRISPFDNQSRRQTSFAEVEVLPVVETTDHID
IPEGDVRVDVYRSSGPGGQSVNTTDSAVRLTHIPSGIVVTCQNEKSQLQNKIAAMRVLQAKLLERKRLEERAELDALKAD
GGSSWGNQMRSYVLHPYQMVKDLRTEYEVGNPAAVLDGDLDGFLEAGIRWRNRRNDD

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=235, Percent_Identity=34.0425531914894, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI34577120, Length=237, Percent_Identity=32.9113924050633, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI166795305, Length=149, Percent_Identity=33.5570469798658, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI2367172, Length=344, Percent_Identity=44.4767441860465, Blast_Score=281, Evalue=6e-77,
Organism=Escherichia coli, GI1787462, Length=244, Percent_Identity=38.1147540983607, Blast_Score=159, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17542784, Length=213, Percent_Identity=30.5164319248826, Blast_Score=88, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6321295, Length=310, Percent_Identity=34.5161290322581, Blast_Score=141, Evalue=1e-34,
Organism=Drosophila melanogaster, GI19921226, Length=287, Percent_Identity=32.404181184669, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 42125; Mature: 41994

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVTLAAVDPDRQADIAALDCTLTTVERVLDVEGLRSRIEKLEHEASDPHLWDDQTRAQR
CCEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
VTSELSHTQGELRRVEELRRRLDDLPVLYELAAEEAGAAAADAVAEADAELKSLRADIEA
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHH
TEVRTLLSGEYDEREALVTIRSGAGGVDAADWAEMLMRMYIRWAEQHKYPVEVFDTSYAE
HHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHH
EAGIKSATFAVHAPFAYGTLSVEQGTHRLVRISPFDNQSRRQTSFAEVEVLPVVETTDHI
HCCCCCEEEEEECCCEECEEEECCCCCEEEEECCCCCCHHHHCCHHEEEEEEEEECCCCC
DIPEGDVRVDVYRSSGPGGQSVNTTDSAVRLTHIPSGIVVTCQNEKSQLQNKIAAMRVLQ
CCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHH
AKLLERKRLEERAELDALKADGGSSWGNQMRSYVLHPYQMVKDLRTEYEVGNPAAVLDGD
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEECCC
LDGFLEAGIRWRNRRNDD
HHHHHHHCHHHCCCCCCC
>Mature Secondary Structure 
PVTLAAVDPDRQADIAALDCTLTTVERVLDVEGLRSRIEKLEHEASDPHLWDDQTRAQR
CEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
VTSELSHTQGELRRVEELRRRLDDLPVLYELAAEEAGAAAADAVAEADAELKSLRADIEA
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHH
TEVRTLLSGEYDEREALVTIRSGAGGVDAADWAEMLMRMYIRWAEQHKYPVEVFDTSYAE
HHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHH
EAGIKSATFAVHAPFAYGTLSVEQGTHRLVRISPFDNQSRRQTSFAEVEVLPVVETTDHI
HCCCCCEEEEEECCCEECEEEECCCCCEEEEECCCCCCHHHHCCHHEEEEEEEEECCCCC
DIPEGDVRVDVYRSSGPGGQSVNTTDSAVRLTHIPSGIVVTCQNEKSQLQNKIAAMRVLQ
CCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHH
AKLLERKRLEERAELDALKADGGSSWGNQMRSYVLHPYQMVKDLRTEYEVGNPAAVLDGD
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEECCC
LDGFLEAGIRWRNRRNDD
HHHHHHHCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA