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The map label for this gene is ubiE

Identifier: 148660327

GI number: 148660327

Start: 650999

End: 651703

Strand: Direct

Name: ubiE

Synonym: MRA_0565

Alternate gene names: 148660327

Gene position: 650999-651703 (Clockwise)

Preceding gene: 148660326

Following gene: 148660331

Centisome position: 14.73

GC content: 66.81

Gene sequence:

>705_bases
ATGAGTCGCGCCGCCTTGGACAAGGATCCCCGCGACGTGGCGTCGATGTTCGATGGCGTCGCCCGCAAGTATGACCTGAC
CAATACCGTGTTGTCCCTGGGCCAGGACCGGTATTGGCGGCGAGCCACTCGGTCGGCGCTGCGGATCGGGCCCGGCCAAA
AGGTCCTGGACCTGGCCGCGGGCACCGCCGTGTCCACCGTAGAGCTCACCAAATCGGGCGCGTGGTGTGTGGCTGCCGAT
TTTTCGGTCGGCATGCTTGCGGCGGGCGCTGCGCGCAAGGTTCCCAAGGTCGCCGGTGACGCCACCCGGCTGCCGTTTGG
TGACGACGTGTTCGATGCGGTCACCATCAGTTTCGGGCTGCGTAACGTCGCAAACCAGCAAGCGGCGCTGCGGGAAATGG
CTCGTGTCACCCGGCCGGGCGGGCGGCTACTAGTGTGCGAATTCTCCACGCCCACCAATGCGTTGTTCGCCACCGCCTAC
AAGGAATACTTGATGCGGGCGCTGCCCCGGGTGGCGCGGGCGGTGTCTAGCAACCCCGAGGCCTACGAGTACCTCGCGGA
GTCGATCAGGGCCTGGCCCGACCAGGCGGTGCTGGCGCACCAGATTTCGCGGGCCGGGTGGTCGGGGGTGCGGTGGCGCA
ACCTGACCGGCGGCATCGTAGCTCTGCATGCCGGATACAAACCCGGCAAACAAACCCCGCAGTGA

Upstream 100 bases:

>100_bases
GGCCGCAGTTGGCCGGTGGTCTGCGATGAGCTGCTCGGCCACTACGAGGCGGTGCGAGGTCGGCGCACGACCCAGGCCGC
GTAACGGTAGCGTCGAGGCT

Downstream 100 bases:

>100_bases
CCGGTAGGAAGACTTAGCGGGTGCCAGCCCGTTGCAGGACGCCCACATGCTCAGGGCAGTAGTGATCGATCGCGGCGCCC
AGGAACTGAAACGCTTGGCC

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MSRAALDKDPRDVASMFDGVARKYDLTNTVLSLGQDRYWRRATRSALRIGPGQKVLDLAAGTAVSTVELTKSGAWCVAAD
FSVGMLAAGAARKVPKVAGDATRLPFGDDVFDAVTISFGLRNVANQQAALREMARVTRPGGRLLVCEFSTPTNALFATAY
KEYLMRALPRVARAVSSNPEAYEYLAESIRAWPDQAVLAHQISRAGWSGVRWRNLTGGIVALHAGYKPGKQTPQ

Sequences:

>Translated_234_residues
MSRAALDKDPRDVASMFDGVARKYDLTNTVLSLGQDRYWRRATRSALRIGPGQKVLDLAAGTAVSTVELTKSGAWCVAAD
FSVGMLAAGAARKVPKVAGDATRLPFGDDVFDAVTISFGLRNVANQQAALREMARVTRPGGRLLVCEFSTPTNALFATAY
KEYLMRALPRVARAVSSNPEAYEYLAESIRAWPDQAVLAHQISRAGWSGVRWRNLTGGIVALHAGYKPGKQTPQ
>Mature_233_residues
SRAALDKDPRDVASMFDGVARKYDLTNTVLSLGQDRYWRRATRSALRIGPGQKVLDLAAGTAVSTVELTKSGAWCVAADF
SVGMLAAGAARKVPKVAGDATRLPFGDDVFDAVTISFGLRNVANQQAALREMARVTRPGGRLLVCEFSTPTNALFATAYK
EYLMRALPRVARAVSSNPEAYEYLAESIRAWPDQAVLAHQISRAGWSGVRWRNLTGGIVALHAGYKPGKQTPQ

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2)

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family

Homologues:

Organism=Homo sapiens, GI116063536, Length=259, Percent_Identity=33.5907335907336, Blast_Score=148, Evalue=4e-36,
Organism=Escherichia coli, GI48994983, Length=226, Percent_Identity=43.8053097345133, Blast_Score=182, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17557039, Length=230, Percent_Identity=33.9130434782609, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6323526, Length=243, Percent_Identity=32.5102880658436, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24641702, Length=246, Percent_Identity=32.520325203252, Blast_Score=135, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIE_MYCBO (P0A639)

Other databases:

- EMBL:   BX248335
- RefSeq:   NP_854233.1
- ProteinModelPortal:   P0A639
- SMR:   P0A639
- EnsemblBacteria:   EBMYCT00000016021
- GeneID:   1091654
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb0573
- GeneTree:   EBGT00050000014588
- HOGENOM:   HBG559580
- OMA:   FDVYTIA
- ProtClustDB:   PRK00216
- BioCyc:   MBOV233413:MB0573-MONOMER
- HAMAP:   MF_01813
- InterPro:   IPR004033
- PANTHER:   PTHR10108:SF24
- TIGRFAMs:   TIGR01934

Pfam domain/function: PF01209 Ubie_methyltran

EC number: 2.1.1.-

Molecular weight: Translated: 25299; Mature: 25168

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: PS01183 UBIE_1; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRAALDKDPRDVASMFDGVARKYDLTNTVLSLGQDRYWRRATRSALRIGPGQKVLDLAA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHC
GTAVSTVELTKSGAWCVAADFSVGMLAAGAARKVPKVAGDATRLPFGDDVFDAVTISFGL
CCHHEEEEEECCCCEEEEECCCHHHHHHHHHHHCCHHCCCCCCCCCCCHHHHHHHHHHHH
RNVANQQAALREMARVTRPGGRLLVCEFSTPTNALFATAYKEYLMRALPRVARAVSSNPE
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AYEYLAESIRAWPDQAVLAHQISRAGWSGVRWRNLTGGIVALHAGYKPGKQTPQ
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEECCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SRAALDKDPRDVASMFDGVARKYDLTNTVLSLGQDRYWRRATRSALRIGPGQKVLDLAA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHC
GTAVSTVELTKSGAWCVAADFSVGMLAAGAARKVPKVAGDATRLPFGDDVFDAVTISFGL
CCHHEEEEEECCCCEEEEECCCHHHHHHHHHHHCCHHCCCCCCCCCCCHHHHHHHHHHHH
RNVANQQAALREMARVTRPGGRLLVCEFSTPTNALFATAYKEYLMRALPRVARAVSSNPE
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AYEYLAESIRAWPDQAVLAHQISRAGWSGVRWRNLTGGIVALHAGYKPGKQTPQ
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEECCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972