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The map label for this gene is pimB

Identifier: 148660326

GI number: 148660326

Start: 649846

End: 650982

Strand: Direct

Name: pimB

Synonym: MRA_0564

Alternate gene names: 148660326

Gene position: 649846-650982 (Clockwise)

Preceding gene: 148660325

Following gene: 148660327

Centisome position: 14.7

GC content: 66.93

Gene sequence:

>1137_bases
GTGTGTGGCGTGCGCGTTGCGATCGTCGCCGAGTCGTTCCTCCCGCAGGTGAACGGCGTCAGCAACTCGGTGGTCAAGGT
ACTCGAACATCTGCGTCGAACCGGTCATGAAGCCCTGGTGATCGCGCCCGACACGCCGCCAGGTGAAGACCGCGCCGAGC
GACTTCACGACGGTGTCCGGGTGCACCGGGTGCCGTCGCGGATGTTCCCAAAGGTGACCACGTTGCCGCTCGGCGTGCCC
ACCTTCCGAATGCTGAGAGCGCTGCGCGGATTCGATCCGGATGTCGTGCATCTGGCGTCGCCGGCGCTGCTTGGCTACGG
TGGACTCCATGCCGCTCGGCGGCTAGGGGTGCCCACGGTCGCGGTCTACCAAACCGATGTTCCGGGTTTCGCGTCCAGCT
ACGGCATTCCGATGACAGCACGGGCGGCGTGGGCATGGTTCCGCCACTTGCATCGCCTGGCTGACCGCACTCTGGCGCCG
TCCACAGCGACAATGGAATCCCTTATTGCCCAGGGCATTCCGCGAGTACACCGGTGGGCACGCGGGGTGGACGTGCAACG
TTTCGCGCCGTCGGCGCGAAACGAGGTGTTGAGGCGACGGTGGTCACCGGACGGCAAACCCATCGTCGGCTTTGTGGGTC
GGCTTGCTCCGGAGAAGCATGTCGACCGGCTCACGGGTCTGGCGGCCTCCGGCGCCGTGCGGCTGGTGATCGTCGGCGAC
GGCATCGACCGGGCAAGATTGCAATCAGCAATGCCCACAGCGGTTTTCACCGGAGCACGGTATGGCAAAGAGCTCGCCGA
GGCGTATGCCAGCATGGACGTCTTCGTACATTCCGGTGAGCACGAGACGTTCTGCCAAGTCGTGCAGGAAGCGCTGGCGT
CGGGGCTACCGGTGATCGCTCCGGACGCCGGCGGACCGCGTGATCTGATAACCCCGCACCGCACCGGGCTGCTGTTGCCG
GTCGGCGAGTTCGAGCACCGGCTTCCTGACGCCGTCGCCCACCTGGTGCACGAACGCCAGCGCTACGCGCTGGCCGCCCG
GCGCAGTGTGCTGGGCCGCAGTTGGCCGGTGGTCTGCGATGAGCTGCTCGGCCACTACGAGGCGGTGCGAGGTCGGCGCA
CGACCCAGGCCGCGTAA

Upstream 100 bases:

>100_bases
TTGGAACGTCGTGAAAATTCGGCGTCTGCAACGGGCTGAGCAGCAGAGTTCGCACGCCGTATGCCGCTACGCAACCATTT
CGACAGCCGGCGCTGACAGT

Downstream 100 bases:

>100_bases
CGGTAGCGTCGAGGCTATGAGTCGCGCCGCCTTGGACAAGGATCCCCGCGACGTGGCGTCGATGTTCGATGGCGTCGCCC
GCAAGTATGACCTGACCAAT

Product: mannosyltransferase PimB

Products: NA

Alternate protein names: Guanosine diphosphomannose-dependent alpha-mannosyltransferase

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MCGVRVAIVAESFLPQVNGVSNSVVKVLEHLRRTGHEALVIAPDTPPGEDRAERLHDGVRVHRVPSRMFPKVTTLPLGVP
TFRMLRALRGFDPDVVHLASPALLGYGGLHAARRLGVPTVAVYQTDVPGFASSYGIPMTARAAWAWFRHLHRLADRTLAP
STATMESLIAQGIPRVHRWARGVDVQRFAPSARNEVLRRRWSPDGKPIVGFVGRLAPEKHVDRLTGLAASGAVRLVIVGD
GIDRARLQSAMPTAVFTGARYGKELAEAYASMDVFVHSGEHETFCQVVQEALASGLPVIAPDAGGPRDLITPHRTGLLLP
VGEFEHRLPDAVAHLVHERQRYALAARRSVLGRSWPVVCDELLGHYEAVRGRRTTQAA

Sequences:

>Translated_378_residues
MCGVRVAIVAESFLPQVNGVSNSVVKVLEHLRRTGHEALVIAPDTPPGEDRAERLHDGVRVHRVPSRMFPKVTTLPLGVP
TFRMLRALRGFDPDVVHLASPALLGYGGLHAARRLGVPTVAVYQTDVPGFASSYGIPMTARAAWAWFRHLHRLADRTLAP
STATMESLIAQGIPRVHRWARGVDVQRFAPSARNEVLRRRWSPDGKPIVGFVGRLAPEKHVDRLTGLAASGAVRLVIVGD
GIDRARLQSAMPTAVFTGARYGKELAEAYASMDVFVHSGEHETFCQVVQEALASGLPVIAPDAGGPRDLITPHRTGLLLP
VGEFEHRLPDAVAHLVHERQRYALAARRSVLGRSWPVVCDELLGHYEAVRGRRTTQAA
>Mature_378_residues
MCGVRVAIVAESFLPQVNGVSNSVVKVLEHLRRTGHEALVIAPDTPPGEDRAERLHDGVRVHRVPSRMFPKVTTLPLGVP
TFRMLRALRGFDPDVVHLASPALLGYGGLHAARRLGVPTVAVYQTDVPGFASSYGIPMTARAAWAWFRHLHRLADRTLAP
STATMESLIAQGIPRVHRWARGVDVQRFAPSARNEVLRRRWSPDGKPIVGFVGRLAPEKHVDRLTGLAASGAVRLVIVGD
GIDRARLQSAMPTAVFTGARYGKELAEAYASMDVFVHSGEHETFCQVVQEALASGLPVIAPDAGGPRDLITPHRTGLLLP
VGEFEHRLPDAVAHLVHERQRYALAARRSVLGRSWPVVCDELLGHYEAVRGRRTTQAA

Specific function: Catalyzes the addition of a mannose residue from GDP-D- mannose to GlcAGroAc2 to generate 1,2-di-O-C16/C18:1-(alpha-D- mannopyranosyl)-(1-4)-(alpha-D-glucopyranosyluronic acid)-(1-3)- glycerol(ManGlcAGroAc2)

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MGTA_MYCTU (O06423)

Other databases:

- EMBL:   AF061562
- EMBL:   BX842573
- EMBL:   AE000516
- PIR:   H70548
- RefSeq:   NP_215071.1
- RefSeq:   NP_334992.1
- ProteinModelPortal:   O06423
- EnsemblBacteria:   EBMYCT00000001864
- EnsemblBacteria:   EBMYCT00000069870
- GeneID:   887609
- GeneID:   924956
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT0583
- KEGG:   mtu:Rv0557
- TIGR:   MT0583
- TubercuList:   Rv0557
- GeneTree:   EBGT00050000015522
- HOGENOM:   HBG726846
- OMA:   WDEIFRG
- ProtClustDB:   CLSK790592
- GO:   GO:0040007
- GO:   GO:0009405
- InterPro:   IPR001296

Pfam domain/function: PF00534 Glycos_transf_1

EC number: NA

Molecular weight: Translated: 41238; Mature: 41238

Theoretical pI: Translated: 10.64; Mature: 10.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGVRVAIVAESFLPQVNGVSNSVVKVLEHLRRTGHEALVIAPDTPPGEDRAERLHDGVR
CCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHCCHH
VHRVPSRMFPKVTTLPLGVPTFRMLRALRGFDPDVVHLASPALLGYGGLHAARRLGVPTV
EEECCHHHCCCCEECCCCCHHHHHHHHHHCCCCCHHHHCCCHHHCCCHHHHHHHCCCCEE
AVYQTDVPGFASSYGIPMTARAAWAWFRHLHRLADRTLAPSTATMESLIAQGIPRVHRWA
EEEECCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHH
RGVDVQRFAPSARNEVLRRRWSPDGKPIVGFVGRLAPEKHVDRLTGLAASGAVRLVIVGD
HCCCHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEEC
GIDRARLQSAMPTAVFTGARYGKELAEAYASMDVFVHSGEHETFCQVVQEALASGLPVIA
CCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHCCCCEEE
PDAGGPRDLITPHRTGLLLPVGEFEHRLPDAVAHLVHERQRYALAARRSVLGRSWPVVCD
CCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
ELLGHYEAVRGRRTTQAA
HHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MCGVRVAIVAESFLPQVNGVSNSVVKVLEHLRRTGHEALVIAPDTPPGEDRAERLHDGVR
CCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHCCHH
VHRVPSRMFPKVTTLPLGVPTFRMLRALRGFDPDVVHLASPALLGYGGLHAARRLGVPTV
EEECCHHHCCCCEECCCCCHHHHHHHHHHCCCCCHHHHCCCHHHCCCHHHHHHHCCCCEE
AVYQTDVPGFASSYGIPMTARAAWAWFRHLHRLADRTLAPSTATMESLIAQGIPRVHRWA
EEEECCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHH
RGVDVQRFAPSARNEVLRRRWSPDGKPIVGFVGRLAPEKHVDRLTGLAASGAVRLVIVGD
HCCCHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEEC
GIDRARLQSAMPTAVFTGARYGKELAEAYASMDVFVHSGEHETFCQVVQEALASGLPVIA
CCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHCCCCEEE
PDAGGPRDLITPHRTGLLLPVGEFEHRLPDAVAHLVHERQRYALAARRSVLGRSWPVVCD
CCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
ELLGHYEAVRGRRTTQAA
HHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10531370; 9634230; 12218036