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The map label for this gene is pckA [H]

Identifier: 148550827

GI number: 148550827

Start: 298815

End: 300509

Strand: Reverse

Name: pckA [H]

Synonym: Swit_5391

Alternate gene names: 148550827

Gene position: 300509-298815 (Counterclockwise)

Preceding gene: 148550833

Following gene: 148550826

Centisome position: 96.87

GC content: 61.65

Gene sequence:

>1695_bases
ATGACGTATGAAACATTAAATTGCAATCTTATCAATCACAGGTCATGTGTGATAAGGCTTGAGAAACCGACTCTCAAGGA
TCGACCATTGGCCTCCCAAGACTCTCCCATCGCTTTCGCGCCTGACGCTGCCAGCACGCTGCATCGCAATCTGGGCACTG
CGCCCCTGATCGAGCATGCGCTGCGCCGGGAAGAGGGCGTGCTTTCGCGCGACGGGGCCTTCGTCGTCGCGACGGGCAAG
CATACGGGTCGTTCGGCGCAGGACAAGTTCATCGTCCAGGACGCCGAGACCGAGGCGGCGATCTGGTGGGGCAAGACCAA
TGTCGCCATGTCGCCCGAGCATTTCGCGGCGCTCAAGCAAGACTTCCTGGACGCGCTGGGCGCCAAGGACACCGTCTTTC
TTCAGGATCTCTTCGGGGGCTCGCAGAGCGAGCATCGCATTGCGGTACGGGTCTACACCGAGCTCGCCTGGCACAGTCTT
TTTGTTCGGACCCTGCTGGTTCGTCCCAGCACCGATGAGCTCGCGACGTTCGTGCCTGAATATACGATCATCGACCTGCC
GAGCTTCCGGGCCGATCCTGAACGCCATGGCTGCCGAAGCGAAACCGTGATCGCGGTCAATTTCGCGCAGAAGCTCATTC
TGATCGGCGGTACGGCCTATGCCGGCGAGATGAAGAAGTCCGTCTTCAGTCTCCTCAATTACCTGCTGCCCGAAAAGGGC
GTGATGCCGATGCATTGCTCGGCCAACATTGGCCCTGAGGGCGACACAGCCGTTTTCTTCGGCCTTTCGGGGACCGGCAA
GACGACCTTGTCGGCCGATGCGAGCCGCACGCTGATCGGCGACGACGAGCATGGCTGGTCGGACAGCGCCATCTTCAACT
TCGAGGGCGGCTGCTATGCCAAGGTGATCCGTCTGTCGGCCGAGGCGGAGCCCGAGATCTACGCCACCACCCGCCGCTTC
GGCACGATCCTCGAAAATGTGGTGATCGATCCCGAAACGCGGATCATCGACCTGGATGATGACAGTCTCGCCGAAAACAG
CCGGGCCTCCTATCCGATCGAATTCATTCCCAACGCGTCGGCCGACAATCTCGGGCCTGTTCCTCGCAACATCATCTTCC
TGACAGCCGATGCCTACGGCGTCCTGCCTCCAATCGCGCGGCTGACGCCCGAGCAGGCCATGTATCATTTCCTCTCGGGC
TACACGGCGCGCGTGGCAGGCACCGAGATCGGCGTGACCGAACCCACCGCAACCTTTTCCACCTGCTTCGGCGCGCCGTT
CATGCCGCGTCATCCGTCCGTCTACGGCAATCTGCTCAAGGATCGCATCGCGCGAGGTGACGTCAAATGCTGGCTCGTCA
ATACGGGCTGGACCGGCGGGAGCTTCGGCACCGGAAAACGCATGCCGATCAAGGTGACCCGTGCTCTTCTCAATGCCGCG
CTCGACGGCAATCTCGACGGCGTCACCTGCCGGATCGACCCGATATTTCGGTTTCAGGTTCCCGTCGCCGCGCCCGATGT
CGACGACACCATCCTCGATCCGCGCGAAACATGGGCGGACAAGGCTGCCTATGACGCAACCGCGGCCAAGCTGGCCCGCC
TGTTCGCCGAGAATTTCGTGCAGTTCGAGGATGCCGTCGATCTCTCTGTCCGCAATGCCGGGCCGCGCGTCGAACTTCGC
GCCGCCGACGCTTGA

Upstream 100 bases:

>100_bases
GTCGGCAGCGAAAGCTGCGCGAGCTGGGCGAGTTGGCGCTGGTCAATCCCGAGAAGGGCGCGAGCCGCACGCATCTGTTG
CGAAGTGATCATGCACCCTC

Downstream 100 bases:

>100_bases
TCACCAACGTCTCGATAAGGGAACGGCCTTCCAGCCTGCTTGTGCTATGACAAGGACCATGACCACCGCGATTCCCCACG
CTTTCGAGATCGGCATCGAT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 564; Mature: 563

Protein sequence:

>564_residues
MTYETLNCNLINHRSCVIRLEKPTLKDRPLASQDSPIAFAPDAASTLHRNLGTAPLIEHALRREEGVLSRDGAFVVATGK
HTGRSAQDKFIVQDAETEAAIWWGKTNVAMSPEHFAALKQDFLDALGAKDTVFLQDLFGGSQSEHRIAVRVYTELAWHSL
FVRTLLVRPSTDELATFVPEYTIIDLPSFRADPERHGCRSETVIAVNFAQKLILIGGTAYAGEMKKSVFSLLNYLLPEKG
VMPMHCSANIGPEGDTAVFFGLSGTGKTTLSADASRTLIGDDEHGWSDSAIFNFEGGCYAKVIRLSAEAEPEIYATTRRF
GTILENVVIDPETRIIDLDDDSLAENSRASYPIEFIPNASADNLGPVPRNIIFLTADAYGVLPPIARLTPEQAMYHFLSG
YTARVAGTEIGVTEPTATFSTCFGAPFMPRHPSVYGNLLKDRIARGDVKCWLVNTGWTGGSFGTGKRMPIKVTRALLNAA
LDGNLDGVTCRIDPIFRFQVPVAAPDVDDTILDPRETWADKAAYDATAAKLARLFAENFVQFEDAVDLSVRNAGPRVELR
AADA

Sequences:

>Translated_564_residues
MTYETLNCNLINHRSCVIRLEKPTLKDRPLASQDSPIAFAPDAASTLHRNLGTAPLIEHALRREEGVLSRDGAFVVATGK
HTGRSAQDKFIVQDAETEAAIWWGKTNVAMSPEHFAALKQDFLDALGAKDTVFLQDLFGGSQSEHRIAVRVYTELAWHSL
FVRTLLVRPSTDELATFVPEYTIIDLPSFRADPERHGCRSETVIAVNFAQKLILIGGTAYAGEMKKSVFSLLNYLLPEKG
VMPMHCSANIGPEGDTAVFFGLSGTGKTTLSADASRTLIGDDEHGWSDSAIFNFEGGCYAKVIRLSAEAEPEIYATTRRF
GTILENVVIDPETRIIDLDDDSLAENSRASYPIEFIPNASADNLGPVPRNIIFLTADAYGVLPPIARLTPEQAMYHFLSG
YTARVAGTEIGVTEPTATFSTCFGAPFMPRHPSVYGNLLKDRIARGDVKCWLVNTGWTGGSFGTGKRMPIKVTRALLNAA
LDGNLDGVTCRIDPIFRFQVPVAAPDVDDTILDPRETWADKAAYDATAAKLARLFAENFVQFEDAVDLSVRNAGPRVELR
AADA
>Mature_563_residues
TYETLNCNLINHRSCVIRLEKPTLKDRPLASQDSPIAFAPDAASTLHRNLGTAPLIEHALRREEGVLSRDGAFVVATGKH
TGRSAQDKFIVQDAETEAAIWWGKTNVAMSPEHFAALKQDFLDALGAKDTVFLQDLFGGSQSEHRIAVRVYTELAWHSLF
VRTLLVRPSTDELATFVPEYTIIDLPSFRADPERHGCRSETVIAVNFAQKLILIGGTAYAGEMKKSVFSLLNYLLPEKGV
MPMHCSANIGPEGDTAVFFGLSGTGKTTLSADASRTLIGDDEHGWSDSAIFNFEGGCYAKVIRLSAEAEPEIYATTRRFG
TILENVVIDPETRIIDLDDDSLAENSRASYPIEFIPNASADNLGPVPRNIIFLTADAYGVLPPIARLTPEQAMYHFLSGY
TARVAGTEIGVTEPTATFSTCFGAPFMPRHPSVYGNLLKDRIARGDVKCWLVNTGWTGGSFGTGKRMPIKVTRALLNAAL
DGNLDGVTCRIDPIFRFQVPVAAPDVDDTILDPRETWADKAAYDATAAKLARLFAENFVQFEDAVDLSVRNAGPRVELRA
ADA

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=508, Percent_Identity=48.8188976377953, Blast_Score=466, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6322950, Length=523, Percent_Identity=44.9330783938815, Blast_Score=468, Evalue=1e-132,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 61590; Mature: 61459

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYETLNCNLINHRSCVIRLEKPTLKDRPLASQDSPIAFAPDAASTLHRNLGTAPLIEHA
CCEEEEEEEEEECCEEEEEECCCCCCCCCCCCCCCCEEECCCHHHHHHHHCCCHHHHHHH
LRREEGVLSRDGAFVVATGKHTGRSAQDKFIVQDAETEAAIWWGKTNVAMSPEHFAALKQ
HHHHCCCEECCCEEEEEECCCCCCCCCCCEEEEECCCCEEEEECCCCCEECHHHHHHHHH
DFLDALGAKDTVFLQDLFGGSQSEHRIAVRVYTELAWHSLFVRTLLVRPSTDELATFVPE
HHHHHHCCCCEEEHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHCCC
YTIIDLPSFRADPERHGCRSETVIAVNFAQKLILIGGTAYAGEMKKSVFSLLNYLLPEKG
EEEEECCCCCCCHHHCCCCCCCEEEEEECCEEEEECCCHHHHHHHHHHHHHHHHHCCCCC
VMPMHCSANIGPEGDTAVFFGLSGTGKTTLSADASRTLIGDDEHGWSDSAIFNFEGGCYA
CCEEEECCCCCCCCCCEEEEEECCCCCEEEECCCCCEEECCCCCCCCCCEEEEECCCCEE
KVIRLSAEAEPEIYATTRRFGTILENVVIDPETRIIDLDDDSLAENSRASYPIEFIPNAS
EEEEECCCCCCCEEEHHHHHHHHHHHEEECCCCEEEECCCCHHCCCCCCCCCEEECCCCC
ADNLGPVPRNIIFLTADAYGVLPPIARLTPEQAMYHFLSGYTARVAGTEIGVTEPTATFS
CCCCCCCCCEEEEEEECCCCCCCCHHHCCHHHHHHHHHCCCEEEECCCCCCCCCCCCHHH
TCFGAPFMPRHPSVYGNLLKDRIARGDVKCWLVNTGWTGGSFGTGKRMPIKVTRALLNAA
HHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
LDGNLDGVTCRIDPIFRFQVPVAAPDVDDTILDPRETWADKAAYDATAAKLARLFAENFV
HCCCCCEEEEEECEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QFEDAVDLSVRNAGPRVELRAADA
HHHHHHCEEEECCCCEEEEEECCC
>Mature Secondary Structure 
TYETLNCNLINHRSCVIRLEKPTLKDRPLASQDSPIAFAPDAASTLHRNLGTAPLIEHA
CEEEEEEEEEECCEEEEEECCCCCCCCCCCCCCCCEEECCCHHHHHHHHCCCHHHHHHH
LRREEGVLSRDGAFVVATGKHTGRSAQDKFIVQDAETEAAIWWGKTNVAMSPEHFAALKQ
HHHHCCCEECCCEEEEEECCCCCCCCCCCEEEEECCCCEEEEECCCCCEECHHHHHHHHH
DFLDALGAKDTVFLQDLFGGSQSEHRIAVRVYTELAWHSLFVRTLLVRPSTDELATFVPE
HHHHHHCCCCEEEHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHCCC
YTIIDLPSFRADPERHGCRSETVIAVNFAQKLILIGGTAYAGEMKKSVFSLLNYLLPEKG
EEEEECCCCCCCHHHCCCCCCCEEEEEECCEEEEECCCHHHHHHHHHHHHHHHHHCCCCC
VMPMHCSANIGPEGDTAVFFGLSGTGKTTLSADASRTLIGDDEHGWSDSAIFNFEGGCYA
CCEEEECCCCCCCCCCEEEEEECCCCCEEEECCCCCEEECCCCCCCCCCEEEEECCCCEE
KVIRLSAEAEPEIYATTRRFGTILENVVIDPETRIIDLDDDSLAENSRASYPIEFIPNAS
EEEEECCCCCCCEEEHHHHHHHHHHHEEECCCCEEEECCCCHHCCCCCCCCCEEECCCCC
ADNLGPVPRNIIFLTADAYGVLPPIARLTPEQAMYHFLSGYTARVAGTEIGVTEPTATFS
CCCCCCCCCEEEEEEECCCCCCCCHHHCCHHHHHHHHHCCCEEEECCCCCCCCCCCCHHH
TCFGAPFMPRHPSVYGNLLKDRIARGDVKCWLVNTGWTGGSFGTGKRMPIKVTRALLNAA
HHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
LDGNLDGVTCRIDPIFRFQVPVAAPDVDDTILDPRETWADKAAYDATAAKLARLFAENFV
HCCCCCEEEEEECEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QFEDAVDLSVRNAGPRVELRAADA
HHHHHHCEEEECCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA