Click here to switch to the map view.

The map label for this gene is pdhB [H]

Identifier: 148550592

GI number: 148550592

Start: 46055

End: 47425

Strand: Reverse

Name: pdhB [H]

Synonym: Swit_5153

Alternate gene names: 148550592

Gene position: 47425-46055 (Counterclockwise)

Preceding gene: 148550593

Following gene: 148550591

Centisome position: 15.29

GC content: 65.43

Gene sequence:

>1371_bases
ATGGCGATTGAACTGAAGATGCCGGCGCTTTCTCCCACCATGGAGGAGGGTACGCTCGCAAAGTGGCTCGTCAAGGAAGG
CGATGCGGTGAAATCCGGCGACATCCTCGCGGAGATCGAGACCGACAAGGCGACGATGGAATTCGAGGCAGTGGACGAGG
GCATCATCGCCAAGATCGTCATTCCTGAAGGCACCGACGGCGTGAAAGTGGGCGCGGTGATCGCGCTGATCGCTGGCGAG
GGTGAAAGCGCCGTGACGGTCCAAGCCGCCGCCCCCGCTCCCAAGGTCGAGGCACCCGCGCCCAAGGCGGCGGAGCCGGC
CCCGCAGCCGGTTGCGGCGCCTGCGCCCCGCGCGGCGGTTGCCGATCCCGATATTCCCGCCGGCACCGAGATCGTGAAGA
CCACGGTGCGCGAGGCGTTGCGCGACGCCATGGCCGAGGAGATGCGCGCCGACGACCGCGTCTTCGTGATGGGCGAGGAA
GTGGCGCAATATCAGGGCGCCTACAAGGTGACGCAGGGCCTGCTGGAAGAGTTCGGCGATCGCCGCGTCATCGACACCCC
GATCACCGAGTATGGTTTTGCCGGCATAGGCACAGGCGCGGCGATGGGCGGCTTGAAGCCGATCGTCGAGTTTATGACCT
TCAACTTCGCCATGCAGGCGATCGACCACATCATCAACTCGGCGGCCAAGACCAACTATATGTCCGGCGGCCAGATGCGC
TGCCCGGTGGTGTTCCGCGGCCCCAACGGCGCCGCCAGCCGCGTCGGCGCCCAGCACAGCCAGAATTACGGGCCCTGGTA
TGCTGCCGTTCCTGGCCTGATCGTGATCGCGCCTTACTCGGCCGCCGACGCCAAGGGGCTGCTGAAGGCCGCGATCCGCT
CGCCCGATCCGGTCGTGTTCCTGGAAAACGAGTTGCTCTACGGCCAGAGCTTCGACGTGCCGAAGCTGGACGATCATGTC
CTGCCGATTGGCAAGGCCCGCATCGCCCGCGCGGGGCGTGACGTGACCCTGGTGAGCTACTCCATTGGTGTCGGTGTCGC
GCTGGAAGCCGCCGACAAGCTGGCAGATGAAGGTGTTGACGCGGAGGTGATCGACCTGCGCACGCTCCGCCCGCTCGATA
AGGAAACCGTGCTGAAGTCGCTCGCCAAGACCAACAGGATGGTGGTGGTGGAGGAAGGCTGGCCGGTCTGCTCCATCTCC
TCGGAGATCATCGCCATCGCCATGCAGGAAGGCTTCGACGACCTCGACGCCCCGGTGCTCCGCGTCACCAACAAGGACGT
GCCGCTGCCGTATGCCGCGAATCTGGAAAAGGCCGCGCTCATCAAGGCGGATGACGTGGTCGCGGCGGTCAAGCGCGTCC
GTTATCGCTGA

Upstream 100 bases:

>100_bases
AGATCCGCCAGATCGTTCAGGAAGCCGCCGACTTCGCCGAGCAGGCCCCGGAGCCCGAGCTTGCCGAACTCTATACCGAT
GTGCTGGTGGGGCAATATTG

Downstream 100 bases:

>100_bases
CGCCTGCGGAGTTTCGAAAATGCCGATTGAACTCAAGATGCCCGCGTTGTCTCCCACGATGGAGGAGGGTTCGCTCGCCA
AATGGCTGGTGAAGGAGGGC

Product: pyruvate dehydrogenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MAIELKMPALSPTMEEGTLAKWLVKEGDAVKSGDILAEIETDKATMEFEAVDEGIIAKIVIPEGTDGVKVGAVIALIAGE
GESAVTVQAAAPAPKVEAPAPKAAEPAPQPVAAPAPRAAVADPDIPAGTEIVKTTVREALRDAMAEEMRADDRVFVMGEE
VAQYQGAYKVTQGLLEEFGDRRVIDTPITEYGFAGIGTGAAMGGLKPIVEFMTFNFAMQAIDHIINSAAKTNYMSGGQMR
CPVVFRGPNGAASRVGAQHSQNYGPWYAAVPGLIVIAPYSAADAKGLLKAAIRSPDPVVFLENELLYGQSFDVPKLDDHV
LPIGKARIARAGRDVTLVSYSIGVGVALEAADKLADEGVDAEVIDLRTLRPLDKETVLKSLAKTNRMVVVEEGWPVCSIS
SEIIAIAMQEGFDDLDAPVLRVTNKDVPLPYAANLEKAALIKADDVVAAVKRVRYR

Sequences:

>Translated_456_residues
MAIELKMPALSPTMEEGTLAKWLVKEGDAVKSGDILAEIETDKATMEFEAVDEGIIAKIVIPEGTDGVKVGAVIALIAGE
GESAVTVQAAAPAPKVEAPAPKAAEPAPQPVAAPAPRAAVADPDIPAGTEIVKTTVREALRDAMAEEMRADDRVFVMGEE
VAQYQGAYKVTQGLLEEFGDRRVIDTPITEYGFAGIGTGAAMGGLKPIVEFMTFNFAMQAIDHIINSAAKTNYMSGGQMR
CPVVFRGPNGAASRVGAQHSQNYGPWYAAVPGLIVIAPYSAADAKGLLKAAIRSPDPVVFLENELLYGQSFDVPKLDDHV
LPIGKARIARAGRDVTLVSYSIGVGVALEAADKLADEGVDAEVIDLRTLRPLDKETVLKSLAKTNRMVVVEEGWPVCSIS
SEIIAIAMQEGFDDLDAPVLRVTNKDVPLPYAANLEKAALIKADDVVAAVKRVRYR
>Mature_455_residues
AIELKMPALSPTMEEGTLAKWLVKEGDAVKSGDILAEIETDKATMEFEAVDEGIIAKIVIPEGTDGVKVGAVIALIAGEG
ESAVTVQAAAPAPKVEAPAPKAAEPAPQPVAAPAPRAAVADPDIPAGTEIVKTTVREALRDAMAEEMRADDRVFVMGEEV
AQYQGAYKVTQGLLEEFGDRRVIDTPITEYGFAGIGTGAAMGGLKPIVEFMTFNFAMQAIDHIINSAAKTNYMSGGQMRC
PVVFRGPNGAASRVGAQHSQNYGPWYAAVPGLIVIAPYSAADAKGLLKAAIRSPDPVVFLENELLYGQSFDVPKLDDHVL
PIGKARIARAGRDVTLVSYSIGVGVALEAADKLADEGVDAEVIDLRTLRPLDKETVLKSLAKTNRMVVVEEGWPVCSISS
EIIAIAMQEGFDDLDAPVLRVTNKDVPLPYAANLEKAALIKADDVVAAVKRVRYR

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=325, Percent_Identity=55.0769230769231, Blast_Score=374, Evalue=1e-104,
Organism=Homo sapiens, GI291084858, Length=325, Percent_Identity=52, Blast_Score=343, Evalue=3e-94,
Organism=Homo sapiens, GI4557353, Length=312, Percent_Identity=33.3333333333333, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI34101272, Length=312, Percent_Identity=33.3333333333333, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI203098753, Length=99, Percent_Identity=51.5151515151515, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI203098816, Length=80, Percent_Identity=57.5, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI31711992, Length=79, Percent_Identity=49.3670886075949, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI260898739, Length=58, Percent_Identity=63.7931034482759, Blast_Score=79, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17538422, Length=322, Percent_Identity=63.9751552795031, Blast_Score=409, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17506935, Length=315, Percent_Identity=37.4603174603175, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17560088, Length=121, Percent_Identity=44.6280991735537, Blast_Score=89, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6319698, Length=324, Percent_Identity=59.8765432098765, Blast_Score=400, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6324258, Length=135, Percent_Identity=41.4814814814815, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6321632, Length=112, Percent_Identity=36.6071428571429, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21358145, Length=325, Percent_Identity=58.1538461538462, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24650940, Length=325, Percent_Identity=58.1538461538462, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI160714832, Length=301, Percent_Identity=33.2225913621262, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI160714828, Length=301, Percent_Identity=33.2225913621262, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24650943, Length=91, Percent_Identity=67.032967032967, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24650945, Length=91, Percent_Identity=67.032967032967, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI20129315, Length=75, Percent_Identity=54.6666666666667, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24582497, Length=64, Percent_Identity=53.125, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 48329; Mature: 48198

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIELKMPALSPTMEEGTLAKWLVKEGDAVKSGDILAEIETDKATMEFEAVDEGIIAKIV
CEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEECCCCCCEEEEE
IPEGTDGVKVGAVIALIAGEGESAVTVQAAAPAPKVEAPAPKAAEPAPQPVAAPAPRAAV
ECCCCCCEEEEEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ADPDIPAGTEIVKTTVREALRDAMAEEMRADDRVFVMGEEVAQYQGAYKVTQGLLEEFGD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHCCHHHHHHHHHHHHCC
RRVIDTPITEYGFAGIGTGAAMGGLKPIVEFMTFNFAMQAIDHIINSAAKTNYMSGGQMR
CEEECCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
CPVVFRGPNGAASRVGAQHSQNYGPWYAAVPGLIVIAPYSAADAKGLLKAAIRSPDPVVF
CCEEEECCCCHHHHHCCCCCCCCCCCHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCEEE
LENELLYGQSFDVPKLDDHVLPIGKARIARAGRDVTLVSYSIGVGVALEAADKLADEGVD
EECCEEECCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEEECCCCEEHHHHHHHHHCCCC
AEVIDLRTLRPLDKETVLKSLAKTNRMVVVEEGWPVCSISSEIIAIAMQEGFDDLDAPVL
CEEEEHHHCCCCCHHHHHHHHHHCCCEEEEECCCCEEECCCCEEEEEHHCCCCCCCCCEE
RVTNKDVPLPYAANLEKAALIKADDVVAAVKRVRYR
EECCCCCCCCCCCCCCHHEEEEHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIELKMPALSPTMEEGTLAKWLVKEGDAVKSGDILAEIETDKATMEFEAVDEGIIAKIV
EEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEECCCCCCEEEEE
IPEGTDGVKVGAVIALIAGEGESAVTVQAAAPAPKVEAPAPKAAEPAPQPVAAPAPRAAV
ECCCCCCEEEEEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ADPDIPAGTEIVKTTVREALRDAMAEEMRADDRVFVMGEEVAQYQGAYKVTQGLLEEFGD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHCCHHHHHHHHHHHHCC
RRVIDTPITEYGFAGIGTGAAMGGLKPIVEFMTFNFAMQAIDHIINSAAKTNYMSGGQMR
CEEECCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
CPVVFRGPNGAASRVGAQHSQNYGPWYAAVPGLIVIAPYSAADAKGLLKAAIRSPDPVVF
CCEEEECCCCHHHHHCCCCCCCCCCCHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCEEE
LENELLYGQSFDVPKLDDHVLPIGKARIARAGRDVTLVSYSIGVGVALEAADKLADEGVD
EECCEEECCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEEECCCCEEHHHHHHHHHCCCC
AEVIDLRTLRPLDKETVLKSLAKTNRMVVVEEGWPVCSISSEIIAIAMQEGFDDLDAPVL
CEEEEHHHCCCCCHHHHHHHHHHCCCEEEEECCCCEEECCCCEEEEEHHCCCCCCCCCEE
RVTNKDVPLPYAANLEKAALIKADDVVAAVKRVRYR
EECCCCCCCCCCCCCCHHEEEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]