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The map label for this gene is pdhC [H]

Identifier: 148550591

GI number: 148550591

Start: 44773

End: 46035

Strand: Reverse

Name: pdhC [H]

Synonym: Swit_5152

Alternate gene names: 148550591

Gene position: 46035-44773 (Counterclockwise)

Preceding gene: 148550592

Following gene: 148550590

Centisome position: 14.84

GC content: 61.6

Gene sequence:

>1263_bases
ATGCCGATTGAACTCAAGATGCCCGCGTTGTCTCCCACGATGGAGGAGGGTTCGCTCGCCAAATGGCTGGTGAAGGAGGG
CGATGTGGTGAAGTCTGGCGACCTGCTGGCCGAAATCGAAACCGACAAGGCGACGATGGAATTCGAGGCGGTGGACGAAG
GCATTATCGCGAAAATCCTCATCCCCGAAGGTACAGAAGGCGTGAAGGTCGGGACCGTCGTCGCCATGCTTGCCGCCGAA
GGCGAGGATATCACCGCTATCGGGGAAGGGGCTGTTCCCGCGCTTCTGCCGGCCCCCGAGATTGCCGACAAGGTCGCTAC
ATCCATTCCCGCCGCTGTTCCTGTCGCGTCGTCAGCCGCACCCGCAATTGGTGCCAGCCGTATCAAGGCCAGCCCGCTTG
CCCGTCGTCTGGCGGAGGCAAGCGCCATCGATCTTTCTACGATAAGAGGCTCCGGGCCTAATGGCCGCATCGTCAAGGTT
GACATTGATGGCGCGACGCCTGCCTCGATGCCGGCCGTCGCTCCGGGGGTCCCCGCGGCGTTTGCCGCCGCAGTACCGTC
GATCGAACCCGACATTCCACACGAAGTCGTCAAGCTGTCAAACATGCGGAAAGTCATTGCCCGACGGCTCACCGAATCGA
AGCAGCAGGTTCCGCACATCTACCTCACGGCCGATATCCACCTCGATCCATTGCTGAAGCTTCGGGCCGATCTCAACGAC
GGGCTTGCCGAACGCGGTGTCAAGCTCTCGGTCAACGATCTGCTGGTCAAGGCCTTGGCGGCCGCCCTTATCGAAGTGCC
CAGCTGCAATGTGCAGTTTGCCGGCGACAATCTGCTGCGGTTCAGCCGCGTCGATATTTCGGTGGCGGTATCGATCCCCG
GCGGCCTGATCACGCCGATCATCGCGGGCGCGAACGCCAAGGGCGTTGCCGCAATCTCCAGCGAGATGAAGGATAGGGCG
GAGCGCGCGCGCAACGGTAAATTGCAGCCGCATGAATATCAGGGCGGCACCGCCAGCCTGTCGAACATGGGCATGTTCGG
CATCAAGCAGTTCGAGGCTGTCATTAACCCGCCCCAGGCCATGATCATGGCGATCGGCGCGGGCGAAAAGCGGCCTTATG
TAGTGAACAATGCGCTGACCGTTGCCACGGTGATGTCGGTAACCGGCAGCTTCGATCACCGCGCGATCGATGGGGCGGAC
GGTGCGCAACTGATGGCCGCTTTCAAGCGCATCGTTGAGAAGCCGCTGACACTGTTGGCCTGA

Upstream 100 bases:

>100_bases
TATGCCGCGAATCTGGAAAAGGCCGCGCTCATCAAGGCGGATGACGTGGTCGCGGCGGTCAAGCGCGTCCGTTATCGCTG
ACGCCTGCGGAGTTTCGAAA

Downstream 100 bases:

>100_bases
ATCTCAAGGAATTTCCGAACATGGCTGATAGTTACGACGTGATTGTCCTTGGCTCAGGCCCCGGCGGCTATGTCGCCGCG
ATCCGCGCCGCTCAGCTCAA

Product: pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MPIELKMPALSPTMEEGSLAKWLVKEGDVVKSGDLLAEIETDKATMEFEAVDEGIIAKILIPEGTEGVKVGTVVAMLAAE
GEDITAIGEGAVPALLPAPEIADKVATSIPAAVPVASSAAPAIGASRIKASPLARRLAEASAIDLSTIRGSGPNGRIVKV
DIDGATPASMPAVAPGVPAAFAAAVPSIEPDIPHEVVKLSNMRKVIARRLTESKQQVPHIYLTADIHLDPLLKLRADLND
GLAERGVKLSVNDLLVKALAAALIEVPSCNVQFAGDNLLRFSRVDISVAVSIPGGLITPIIAGANAKGVAAISSEMKDRA
ERARNGKLQPHEYQGGTASLSNMGMFGIKQFEAVINPPQAMIMAIGAGEKRPYVVNNALTVATVMSVTGSFDHRAIDGAD
GAQLMAAFKRIVEKPLTLLA

Sequences:

>Translated_420_residues
MPIELKMPALSPTMEEGSLAKWLVKEGDVVKSGDLLAEIETDKATMEFEAVDEGIIAKILIPEGTEGVKVGTVVAMLAAE
GEDITAIGEGAVPALLPAPEIADKVATSIPAAVPVASSAAPAIGASRIKASPLARRLAEASAIDLSTIRGSGPNGRIVKV
DIDGATPASMPAVAPGVPAAFAAAVPSIEPDIPHEVVKLSNMRKVIARRLTESKQQVPHIYLTADIHLDPLLKLRADLND
GLAERGVKLSVNDLLVKALAAALIEVPSCNVQFAGDNLLRFSRVDISVAVSIPGGLITPIIAGANAKGVAAISSEMKDRA
ERARNGKLQPHEYQGGTASLSNMGMFGIKQFEAVINPPQAMIMAIGAGEKRPYVVNNALTVATVMSVTGSFDHRAIDGAD
GAQLMAAFKRIVEKPLTLLA
>Mature_419_residues
PIELKMPALSPTMEEGSLAKWLVKEGDVVKSGDLLAEIETDKATMEFEAVDEGIIAKILIPEGTEGVKVGTVVAMLAAEG
EDITAIGEGAVPALLPAPEIADKVATSIPAAVPVASSAAPAIGASRIKASPLARRLAEASAIDLSTIRGSGPNGRIVKVD
IDGATPASMPAVAPGVPAAFAAAVPSIEPDIPHEVVKLSNMRKVIARRLTESKQQVPHIYLTADIHLDPLLKLRADLNDG
LAERGVKLSVNDLLVKALAAALIEVPSCNVQFAGDNLLRFSRVDISVAVSIPGGLITPIIAGANAKGVAAISSEMKDRAE
RARNGKLQPHEYQGGTASLSNMGMFGIKQFEAVINPPQAMIMAIGAGEKRPYVVNNALTVATVMSVTGSFDHRAIDGADG
AQLMAAFKRIVEKPLTLLA

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=440, Percent_Identity=44.0909090909091, Blast_Score=333, Evalue=3e-91,
Organism=Homo sapiens, GI203098753, Length=456, Percent_Identity=37.9385964912281, Blast_Score=278, Evalue=5e-75,
Organism=Homo sapiens, GI203098816, Length=456, Percent_Identity=37.9385964912281, Blast_Score=278, Evalue=9e-75,
Organism=Homo sapiens, GI110671329, Length=434, Percent_Identity=26.4976958525346, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI260898739, Length=165, Percent_Identity=41.8181818181818, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI19923748, Length=225, Percent_Identity=32, Blast_Score=112, Evalue=6e-25,
Organism=Escherichia coli, GI1786946, Length=421, Percent_Identity=29.6912114014252, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1786305, Length=404, Percent_Identity=28.4653465346535, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17560088, Length=439, Percent_Identity=45.1025056947608, Blast_Score=317, Evalue=7e-87,
Organism=Caenorhabditis elegans, GI17538894, Length=228, Percent_Identity=41.2280701754386, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17537937, Length=433, Percent_Identity=29.0993071593533, Blast_Score=167, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI25146366, Length=421, Percent_Identity=28.7410926365796, Blast_Score=143, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6324258, Length=444, Percent_Identity=39.1891891891892, Blast_Score=282, Evalue=6e-77,
Organism=Saccharomyces cerevisiae, GI6320352, Length=417, Percent_Identity=27.0983213429257, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6321632, Length=83, Percent_Identity=43.3734939759036, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI20129315, Length=439, Percent_Identity=41.2300683371298, Blast_Score=284, Evalue=7e-77,
Organism=Drosophila melanogaster, GI24582497, Length=428, Percent_Identity=40.6542056074766, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI18859875, Length=423, Percent_Identity=29.5508274231678, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24645909, Length=228, Percent_Identity=28.9473684210526, Blast_Score=108, Evalue=8e-24,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 43629; Mature: 43498

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIELKMPALSPTMEEGSLAKWLVKEGDVVKSGDLLAEIETDKATMEFEAVDEGIIAKIL
CCEEEECCCCCCCCCCCCHHHHHHHCCCEEECCCEEEEEECCCCEEEHHHCCCCCEEEEE
IPEGTEGVKVGTVVAMLAAEGEDITAIGEGAVPALLPAPEIADKVATSIPAAVPVASSAA
ECCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCC
PAIGASRIKASPLARRLAEASAIDLSTIRGSGPNGRIVKVDIDGATPASMPAVAPGVPAA
CCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHH
FAAAVPSIEPDIPHEVVKLSNMRKVIARRLTESKQQVPHIYLTADIHLDPLLKLRADLND
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHCCCC
GLAERGVKLSVNDLLVKALAAALIEVPSCNVQFAGDNLLRFSRVDISVAVSIPGGLITPI
CHHHCCCEEEHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEEEEEEEEECCCCHHHHH
IAGANAKGVAAISSEMKDRAERARNGKLQPHEYQGGTASLSNMGMFGIKQFEAVINPPQA
HCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHCCCHH
MIMAIGAGEKRPYVVNNALTVATVMSVTGSFDHRAIDGADGAQLMAAFKRIVEKPLTLLA
EEEEECCCCCCCEEECCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PIELKMPALSPTMEEGSLAKWLVKEGDVVKSGDLLAEIETDKATMEFEAVDEGIIAKIL
CEEEECCCCCCCCCCCCHHHHHHHCCCEEECCCEEEEEECCCCEEEHHHCCCCCEEEEE
IPEGTEGVKVGTVVAMLAAEGEDITAIGEGAVPALLPAPEIADKVATSIPAAVPVASSAA
ECCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCC
PAIGASRIKASPLARRLAEASAIDLSTIRGSGPNGRIVKVDIDGATPASMPAVAPGVPAA
CCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHH
FAAAVPSIEPDIPHEVVKLSNMRKVIARRLTESKQQVPHIYLTADIHLDPLLKLRADLND
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHCCCC
GLAERGVKLSVNDLLVKALAAALIEVPSCNVQFAGDNLLRFSRVDISVAVSIPGGLITPI
CHHHCCCEEEHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEEEEEEEEECCCCHHHHH
IAGANAKGVAAISSEMKDRAERARNGKLQPHEYQGGTASLSNMGMFGIKQFEAVINPPQA
HCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHCCCHH
MIMAIGAGEKRPYVVNNALTVATVMSVTGSFDHRAIDGADGAQLMAAFKRIVEKPLTLLA
EEEEECCCCCCCEEECCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]