BacMap

Definition	Clostridium botulinum A str. ATCC 3502, complete genome.
Accession	NC_009495
Length	3,886,916

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The map label for this gene is steT [H]

Identifier: 148379352

GI number: 148379352

Start: 1500703

End: 1502022

Strand: Direct

Name: steT [H]

Synonym: CBO1377

Alternate gene names: 148379352

Gene position: 1500703-1502022 (Clockwise)

Preceding gene: 148379351

Following gene: 148379353

Centisome position: 38.61

GC content: 30.38

Gene sequence:

>1320_bases
ATGAAAGATGCTAAAGAGGAAAATTTGCAAAAAAACCTAGGATTATTTGATGCTATAGCTATAGTTATAGGTATGGTTAT
AGGATCAGGTATATTTTTTAAGCCAGCTATAGTTTTTAAAAATGCTGGTTCACCAATGTTAGGTATATTGGCTTGGTTAG
TTGGAGGTTTAATTACTATAGCTTCTGGTCTTACAATAGCAGAAATTGCTGCAGCTATTCCTAAGACTGGGGGAATATTT
GTTTATATTAAAGAATTATATGGAGAAAAATTTGCATTTTTAATAGGATGGGTCCAATCAATTGTATATATACCAGGGTC
AGCGGCAGCTTTATCCATAATACTTTCTATACAGATTACTAGTTTTATACCTTTAACAGCTATTGAACAAAAAGTTTTAG
CTATAGTGTTTATAATATTTATCATGATTTTAAATATTATATCTACTAAATTAGTAAGTAAAATGCAAGGGATAATAAGT
ATTGCTAAACTAATACCTATCATCGCTATAATAGCTTTAGGATTTATAAAGGGAACAGCAAAAGGGGATATACTAAATAT
TAGTTCTTCAAGTACTGTATCTGGGTTTGGAGCTGCACTTTTAGGAACTTTATGGGCTTATGATGGATGGATAAGTGTAG
GTAATATGGCAGGAGAACTTAAAAATCCTAAAAAAGATTTGCCAAGATCTATAATAATAGGTTTAGGTACTACTATAGTA
GTGTATGTACTTATAAATTTAGCTCTAATTAATATAATGCCAATGAGTGCAATAATAACATCTGAAAAACCAGCTTCAGA
TGCAGCAGTAGTATTATTAGGAAGTACAGGAGCAAAAATCATTTCAGCAGGAATATTAATTTCTATATTTGGAGCCTTAA
ATGGATATTTGATGACAGGAGTTAGAATACCTTTAGCCATGTCTCAGGATAGGAGCATACCTTTTAGTAAGTATTTTAGT
AAAGTTCATGAAAAATCAGGGACACCAGTTAATGCCTTTATATTTGAAACTGTATTAGCTTGTTTATATGTATTATCAGG
ATCATTTAATGTACTAACGGACTTAGTGATGTTCGTATTATGGCTATTCTTTACTATGGCAGTAGCAGGCATATTTGTTT
TAAGAACTAAATTTAAACATTTGGATAGACCCTACAAAGTACCATTATATCCATTAGTACCTCTTATAGGTATAATAGGA
AGTAGCTATATAATAATAAGTACATTAATAACAAATACTTTCTATGCTCTTTGTGGTATAGGAATAACCTTATTAGGTTT
ACCCGTTTATAGCTATATAAAAAAGCAGAATAAAGTATAA

Upstream 100 bases:

>100_bases
TTATATTTTTAATTTAAGATTATAGACTATAGTATTTTGTGGATAAAATTTTCCACCAAAGTAATTTGATACTAAATACT
AAAGAAAGGGAAGAAATGAT

Downstream 100 bases:

>100_bases
ACATTAGCTCACCATTATATATATTTTGATTTAATATGCAAAGAAAAGCAGATTATAAAAAGTTATGGAACTTAGAATTT
TAAAGGATTCTAGAATTTTA

Product: amino acid permease family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MKDAKEENLQKNLGLFDAIAIVIGMVIGSGIFFKPAIVFKNAGSPMLGILAWLVGGLITIASGLTIAEIAAAIPKTGGIF
VYIKELYGEKFAFLIGWVQSIVYIPGSAAALSIILSIQITSFIPLTAIEQKVLAIVFIIFIMILNIISTKLVSKMQGIIS
IAKLIPIIAIIALGFIKGTAKGDILNISSSSTVSGFGAALLGTLWAYDGWISVGNMAGELKNPKKDLPRSIIIGLGTTIV
VYVLINLALINIMPMSAIITSEKPASDAAVVLLGSTGAKIISAGILISIFGALNGYLMTGVRIPLAMSQDRSIPFSKYFS
KVHEKSGTPVNAFIFETVLACLYVLSGSFNVLTDLVMFVLWLFFTMAVAGIFVLRTKFKHLDRPYKVPLYPLVPLIGIIG
SSYIIISTLITNTFYALCGIGITLLGLPVYSYIKKQNKV

Sequences:

>Translated_439_residues
MKDAKEENLQKNLGLFDAIAIVIGMVIGSGIFFKPAIVFKNAGSPMLGILAWLVGGLITIASGLTIAEIAAAIPKTGGIF
VYIKELYGEKFAFLIGWVQSIVYIPGSAAALSIILSIQITSFIPLTAIEQKVLAIVFIIFIMILNIISTKLVSKMQGIIS
IAKLIPIIAIIALGFIKGTAKGDILNISSSSTVSGFGAALLGTLWAYDGWISVGNMAGELKNPKKDLPRSIIIGLGTTIV
VYVLINLALINIMPMSAIITSEKPASDAAVVLLGSTGAKIISAGILISIFGALNGYLMTGVRIPLAMSQDRSIPFSKYFS
KVHEKSGTPVNAFIFETVLACLYVLSGSFNVLTDLVMFVLWLFFTMAVAGIFVLRTKFKHLDRPYKVPLYPLVPLIGIIG
SSYIIISTLITNTFYALCGIGITLLGLPVYSYIKKQNKV
>Mature_439_residues
MKDAKEENLQKNLGLFDAIAIVIGMVIGSGIFFKPAIVFKNAGSPMLGILAWLVGGLITIASGLTIAEIAAAIPKTGGIF
VYIKELYGEKFAFLIGWVQSIVYIPGSAAALSIILSIQITSFIPLTAIEQKVLAIVFIIFIMILNIISTKLVSKMQGIIS
IAKLIPIIAIIALGFIKGTAKGDILNISSSSTVSGFGAALLGTLWAYDGWISVGNMAGELKNPKKDLPRSIIIGLGTTIV
VYVLINLALINIMPMSAIITSEKPASDAAVVLLGSTGAKIISAGILISIFGALNGYLMTGVRIPLAMSQDRSIPFSKYFS
KVHEKSGTPVNAFIFETVLACLYVLSGSFNVLTDLVMFVLWLFFTMAVAGIFVLRTKFKHLDRPYKVPLYPLVPLIGIIG
SSYIIISTLITNTFYALCGIGITLLGLPVYSYIKKQNKV

Specific function: Exhibits an obligate exchange activity for serine, threonine and aromatic amino acids [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family [H]

Homologues:

Organism=Homo sapiens, GI187423910, Length=426, Percent_Identity=30.9859154929577, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI7657591, Length=426, Percent_Identity=30.9859154929577, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI71979932, Length=450, Percent_Identity=31.1111111111111, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI33286428, Length=438, Percent_Identity=30.8219178082192, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI115648063, Length=440, Percent_Identity=28.1818181818182, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI115648022, Length=440, Percent_Identity=28.1818181818182, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI186910308, Length=437, Percent_Identity=27.9176201372998, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI186910306, Length=437, Percent_Identity=27.9176201372998, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI186910304, Length=437, Percent_Identity=27.9176201372998, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI7657683, Length=437, Percent_Identity=29.0617848970252, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI9790235, Length=437, Percent_Identity=27.4599542334096, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI33286430, Length=261, Percent_Identity=29.8850574712644, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI4507047, Length=426, Percent_Identity=23.4741784037559, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI258614005, Length=427, Percent_Identity=22.7166276346604, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI258614003, Length=415, Percent_Identity=22.6506024096386, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI71999149, Length=426, Percent_Identity=25.1173708920188, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI258645169, Length=415, Percent_Identity=22.6506024096386, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI114326544, Length=411, Percent_Identity=22.1411192214112, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI114326550, Length=411, Percent_Identity=22.1411192214112, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI110347453, Length=353, Percent_Identity=25.2124645892351, Blast_Score=72, Evalue=9e-13,
Organism=Escherichia coli, GI87082250, Length=445, Percent_Identity=30.561797752809, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI2367353, Length=355, Percent_Identity=27.3239436619718, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI87081708, Length=433, Percent_Identity=24.7113163972286, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788480, Length=472, Percent_Identity=23.9406779661017, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786694, Length=450, Percent_Identity=23.5555555555556, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790653, Length=463, Percent_Identity=19.8704103671706, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI87081915, Length=338, Percent_Identity=24.2603550295858, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790575, Length=339, Percent_Identity=23.0088495575221, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1786302, Length=450, Percent_Identity=22.2222222222222, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1786789, Length=410, Percent_Identity=20.4878048780488, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1786908, Length=425, Percent_Identity=22.8235294117647, Blast_Score=62, Evalue=7e-11,
Organism=Escherichia coli, GI1789017, Length=380, Percent_Identity=25.5263157894737, Blast_Score=62, Evalue=7e-11,
Organism=Escherichia coli, GI1790584, Length=352, Percent_Identity=26.7045454545455, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17540018, Length=456, Percent_Identity=30.9210526315789, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17568033, Length=445, Percent_Identity=27.4157303370787, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71995388, Length=458, Percent_Identity=26.4192139737991, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71982612, Length=442, Percent_Identity=26.0180995475113, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI71995382, Length=458, Percent_Identity=26.4192139737991, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI71982608, Length=444, Percent_Identity=25.9009009009009, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI71993915, Length=405, Percent_Identity=27.1604938271605, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32566699, Length=409, Percent_Identity=27.6283618581907, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI193208714, Length=401, Percent_Identity=26.6832917705736, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI32566695, Length=401, Percent_Identity=26.6832917705736, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71990446, Length=454, Percent_Identity=25.1101321585903, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71982623, Length=273, Percent_Identity=26.3736263736264, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71987339, Length=368, Percent_Identity=25, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17531343, Length=405, Percent_Identity=23.7037037037037, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17532491, Length=393, Percent_Identity=22.3918575063613, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6321492, Length=352, Percent_Identity=23.8636363636364, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6321751, Length=396, Percent_Identity=25, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324990, Length=430, Percent_Identity=23.0232558139535, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6321629, Length=362, Percent_Identity=23.2044198895028, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24641993, Length=388, Percent_Identity=30.6701030927835, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24641995, Length=388, Percent_Identity=30.6701030927835, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI45550968, Length=446, Percent_Identity=28.9237668161435, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI19921172, Length=446, Percent_Identity=28.9237668161435, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI21357367, Length=438, Percent_Identity=27.8538812785388, Blast_Score=165, Evalue=6e-41,
Organism=Drosophila melanogaster, GI24651635, Length=443, Percent_Identity=28.8939051918736, Blast_Score=158, Evalue=7e-39,
Organism=Drosophila melanogaster, GI24651633, Length=443, Percent_Identity=28.8939051918736, Blast_Score=158, Evalue=7e-39,
Organism=Drosophila melanogaster, GI221331183, Length=433, Percent_Identity=27.9445727482679, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI17647653, Length=433, Percent_Identity=27.9445727482679, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24664379, Length=433, Percent_Identity=27.9445727482679, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI221512776, Length=417, Percent_Identity=24.220623501199, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24666159, Length=417, Percent_Identity=24.220623501199, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24667468, Length=416, Percent_Identity=25.7211538461538, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24668806, Length=409, Percent_Identity=23.7163814180929, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356285, Length=409, Percent_Identity=23.7163814180929, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24668802, Length=409, Percent_Identity=23.7163814180929, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281366235, Length=375, Percent_Identity=23.7333333333333, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI116007820, Length=375, Percent_Identity=23.7333333333333, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 46953; Mature: 46953

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDAKEENLQKNLGLFDAIAIVIGMVIGSGIFFKPAIVFKNAGSPMLGILAWLVGGLITI
CCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
ASGLTIAEIAAAIPKTGGIFVYIKELYGEKFAFLIGWVQSIVYIPGSAAALSIILSIQIT
HCCCHHHHHHHHCCCCCCEEEEHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
SFIPLTAIEQKVLAIVFIIFIMILNIISTKLVSKMQGIISIAKLIPIIAIIALGFIKGTA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KGDILNISSSSTVSGFGAALLGTLWAYDGWISVGNMAGELKNPKKDLPRSIIIGLGTTIV
CCCEEECCCCCCHHHHHHHHHHHHHHHCCHHHHCCHHHHCCCCHHHCCHHHHHHHHHHHH
VYVLINLALINIMPMSAIITSEKPASDAAVVLLGSTGAKIISAGILISIFGALNGYLMTG
HHHHHHHHHHHHHHHHHHEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHC
VRIPLAMSQDRSIPFSKYFSKVHEKSGTPVNAFIFETVLACLYVLSGSFNVLTDLVMFVL
EEECEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
WLFFTMAVAGIFVLRTKFKHLDRPYKVPLYPLVPLIGIIGSSYIIISTLITNTFYALCGI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
GITLLGLPVYSYIKKQNKV
HHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKDAKEENLQKNLGLFDAIAIVIGMVIGSGIFFKPAIVFKNAGSPMLGILAWLVGGLITI
CCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
ASGLTIAEIAAAIPKTGGIFVYIKELYGEKFAFLIGWVQSIVYIPGSAAALSIILSIQIT
HCCCHHHHHHHHCCCCCCEEEEHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
SFIPLTAIEQKVLAIVFIIFIMILNIISTKLVSKMQGIISIAKLIPIIAIIALGFIKGTA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KGDILNISSSSTVSGFGAALLGTLWAYDGWISVGNMAGELKNPKKDLPRSIIIGLGTTIV
CCCEEECCCCCCHHHHHHHHHHHHHHHCCHHHHCCHHHHCCCCHHHCCHHHHHHHHHHHH
VYVLINLALINIMPMSAIITSEKPASDAAVVLLGSTGAKIISAGILISIFGALNGYLMTG
HHHHHHHHHHHHHHHHHHEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHC
VRIPLAMSQDRSIPFSKYFSKVHEKSGTPVNAFIFETVLACLYVLSGSFNVLTDLVMFVL
EEECEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
WLFFTMAVAGIFVLRTKFKHLDRPYKVPLYPLVPLIGIIGSSYIIISTLITNTFYALCGI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
GITLLGLPVYSYIKKQNKV
HHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]