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The map label for this gene is yaaJ [C]

Identifier: 148379275

GI number: 148379275

Start: 1408041

End: 1409393

Strand: Direct

Name: yaaJ [C]

Synonym: CBO1294

Alternate gene names: 148379275

Gene position: 1408041-1409393 (Clockwise)

Preceding gene: 148379274

Following gene: 148379276

Centisome position: 36.23

GC content: 32.96

Gene sequence:

>1353_bases
ATGGATCTTCTAAATGCAGTTAAAGGAATAAACAATGTACTGTGGAATTATATACTGATTTTTTTGTTGTGTGGAACAGG
AATTGTATTTACTGTGTCTCTAAAATTTATTCAAGTCTCTAAATTTAAGGAATCCTTTAAAAAAGCCTTCGGAGGTATAA
GTTTAAGAGGCAAAAAAGCAGGTAAAGATGGAATGAGCTCATTTCAATCATTAGCAACAGCAGTAGCGGCTCAAGTGGGT
ACAGGTAATTTAGCAGGAGCAGCAACAGCTATAGTATCAGGGGGACCTGGAGCAATTTTTTGGATGTGGATTAGTGCATT
TTTAGGAATGGCAACTATTTTTGGAGAAGCGGTTTTAGCACAGATTTTTAAAGAAAAAGTTAATGGAGAAGTGACAGGGG
GGCCAGCTTATTATATAAGTAAAGGACTGAAAAGTAAATTTTTAGCTAGTTTTTTTTCTGTAACTATAATATTAGCTCTG
GGATTTATAGGCAATATGGTTCAGGCAAATTCTATAGGAGCAGCCTTTTCAAATATTTCTAATATACCATCTTGGATAAT
AGGAATAATGATTGCAATACTTGGACTAGTTGTTTTTGTGGGAGGAATAGGAAGAATTGCTTCCGTAACGGAAAAAATGG
TTCCTATAATGGCAGCATTTTATATTATAGGAAGTATCATAATATTAGCTAAGAATTATACAAATATATTACCTGCTTTT
AAATTAATATTTGTCTCTGCTTTTAATCCTAAGGCTGCTGTAGGCGGAGTAGCAGGTGTTACTGTAAAACAGGCTATACG
ATATGGAGTAGCAAGAGGATTATTCTCCAATGAAGCTGGAATGGGATCAACACCACATGCCCATGCGGTAGCTAAGGTGA
AACATCCAGTAGAACAAGGATTAGTAGCTATAGTTGGAGTATTTATAGATACTTTTATAGTTCTTACATTCACAGCATTT
GTAATACTTACAACAGGAGTATTAGATGGAAAAACAACAGGCATAGAATTGACACAAAATGCATTTACTCAGGGATTAGG
TAATTTTGGTGCATATTTTATAGCCATCGCATTATTTTTCTTTGCTTTTTCAACAATAATTGGATGGTATTTCTTTGGAG
AAGCTAATGTGAAATATCTCTTTAAAGGTAAGGGATTGAATATATATAGATTGTTAGTGGCAATTTTTATAGTGATAGGA
ACAACTTTAAAAGTAGATTTAGTTTGGGAGCTTGCAGATACTTTTAATGGACTTATGGTAATTCCTAATGTAATAGCATT
GGTAGCTTTATCTAAAATAGTAAAAGATTCATTAAAGGATTATAATGAAAATTTTAAAGTTACAGATAAATAA

Upstream 100 bases:

>100_bases
TAAAGAAATATGTTAAAAATGTAACATATTGGCAGGAATTAATATGTTCATATGGAATATATATGTAATGTGTGTATTTA
ATTTACAAGGAGGAGTATAT

Downstream 100 bases:

>100_bases
ATACTAAGGAACTCTAACTATATTAATAAATTAAAGTATTAATATAAGGATATATTTAAAGAATACCAAATTTAAAAGAA
GGTTTTATATATAATAGGCA

Product: sodium/alanine symporter

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MDLLNAVKGINNVLWNYILIFLLCGTGIVFTVSLKFIQVSKFKESFKKAFGGISLRGKKAGKDGMSSFQSLATAVAAQVG
TGNLAGAATAIVSGGPGAIFWMWISAFLGMATIFGEAVLAQIFKEKVNGEVTGGPAYYISKGLKSKFLASFFSVTIILAL
GFIGNMVQANSIGAAFSNISNIPSWIIGIMIAILGLVVFVGGIGRIASVTEKMVPIMAAFYIIGSIIILAKNYTNILPAF
KLIFVSAFNPKAAVGGVAGVTVKQAIRYGVARGLFSNEAGMGSTPHAHAVAKVKHPVEQGLVAIVGVFIDTFIVLTFTAF
VILTTGVLDGKTTGIELTQNAFTQGLGNFGAYFIAIALFFFAFSTIIGWYFFGEANVKYLFKGKGLNIYRLLVAIFIVIG
TTLKVDLVWELADTFNGLMVIPNVIALVALSKIVKDSLKDYNENFKVTDK

Sequences:

>Translated_450_residues
MDLLNAVKGINNVLWNYILIFLLCGTGIVFTVSLKFIQVSKFKESFKKAFGGISLRGKKAGKDGMSSFQSLATAVAAQVG
TGNLAGAATAIVSGGPGAIFWMWISAFLGMATIFGEAVLAQIFKEKVNGEVTGGPAYYISKGLKSKFLASFFSVTIILAL
GFIGNMVQANSIGAAFSNISNIPSWIIGIMIAILGLVVFVGGIGRIASVTEKMVPIMAAFYIIGSIIILAKNYTNILPAF
KLIFVSAFNPKAAVGGVAGVTVKQAIRYGVARGLFSNEAGMGSTPHAHAVAKVKHPVEQGLVAIVGVFIDTFIVLTFTAF
VILTTGVLDGKTTGIELTQNAFTQGLGNFGAYFIAIALFFFAFSTIIGWYFFGEANVKYLFKGKGLNIYRLLVAIFIVIG
TTLKVDLVWELADTFNGLMVIPNVIALVALSKIVKDSLKDYNENFKVTDK
>Mature_450_residues
MDLLNAVKGINNVLWNYILIFLLCGTGIVFTVSLKFIQVSKFKESFKKAFGGISLRGKKAGKDGMSSFQSLATAVAAQVG
TGNLAGAATAIVSGGPGAIFWMWISAFLGMATIFGEAVLAQIFKEKVNGEVTGGPAYYISKGLKSKFLASFFSVTIILAL
GFIGNMVQANSIGAAFSNISNIPSWIIGIMIAILGLVVFVGGIGRIASVTEKMVPIMAAFYIIGSIIILAKNYTNILPAF
KLIFVSAFNPKAAVGGVAGVTVKQAIRYGVARGLFSNEAGMGSTPHAHAVAKVKHPVEQGLVAIVGVFIDTFIVLTFTAF
VILTTGVLDGKTTGIELTQNAFTQGLGNFGAYFIAIALFFFAFSTIIGWYFFGEANVKYLFKGKGLNIYRLLVAIFIVIG
TTLKVDLVWELADTFNGLMVIPNVIALVALSKIVKDSLKDYNENFKVTDK

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=450, Percent_Identity=39.3333333333333, Blast_Score=295, Evalue=5e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 47904; Mature: 47904

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLLNAVKGINNVLWNYILIFLLCGTGIVFTVSLKFIQVSKFKESFKKAFGGISLRGKKA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCEECCCCC
GKDGMSSFQSLATAVAAQVGTGNLAGAATAIVSGGPGAIFWMWISAFLGMATIFGEAVLA
CHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QIFKEKVNGEVTGGPAYYISKGLKSKFLASFFSVTIILALGFIGNMVQANSIGAAFSNIS
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIPSWIIGIMIAILGLVVFVGGIGRIASVTEKMVPIMAAFYIIGSIIILAKNYTNILPAF
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
KLIFVSAFNPKAAVGGVAGVTVKQAIRYGVARGLFSNEAGMGSTPHAHAVAKVKHPVEQG
HHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
LVAIVGVFIDTFIVLTFTAFVILTTGVLDGKTTGIELTQNAFTQGLGNFGAYFIAIALFF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
FAFSTIIGWYFFGEANVKYLFKGKGLNIYRLLVAIFIVIGTTLKVDLVWELADTFNGLMV
HHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHH
IPNVIALVALSKIVKDSLKDYNENFKVTDK
HHHHHHHHHHHHHHHHHHHHCCCCCEECCC
>Mature Secondary Structure
MDLLNAVKGINNVLWNYILIFLLCGTGIVFTVSLKFIQVSKFKESFKKAFGGISLRGKKA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCEECCCCC
GKDGMSSFQSLATAVAAQVGTGNLAGAATAIVSGGPGAIFWMWISAFLGMATIFGEAVLA
CHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QIFKEKVNGEVTGGPAYYISKGLKSKFLASFFSVTIILALGFIGNMVQANSIGAAFSNIS
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIPSWIIGIMIAILGLVVFVGGIGRIASVTEKMVPIMAAFYIIGSIIILAKNYTNILPAF
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
KLIFVSAFNPKAAVGGVAGVTVKQAIRYGVARGLFSNEAGMGSTPHAHAVAKVKHPVEQG
HHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
LVAIVGVFIDTFIVLTFTAFVILTTGVLDGKTTGIELTQNAFTQGLGNFGAYFIAIALFF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
FAFSTIIGWYFFGEANVKYLFKGKGLNIYRLLVAIFIVIGTTLKVDLVWELADTFNGLMV
HHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHH
IPNVIALVALSKIVKDSLKDYNENFKVTDK
HHHHHHHHHHHHHHHHHHHHCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]