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The map label for this gene is rnjA [H]

Identifier: 148379274

GI number: 148379274

Start: 1406072

End: 1407736

Strand: Direct

Name: rnjA [H]

Synonym: CBO1293

Alternate gene names: 148379274

Gene position: 1406072-1407736 (Clockwise)

Preceding gene: 148379273

Following gene: 148379275

Centisome position: 36.17

GC content: 32.07

Gene sequence:

>1665_bases
GTGAGCAATAAAGATAGTGTAAAGGTTATTCCTTTGGGTGGACTTGGAGAAATTGGTAAAAATATAACTGCTTTTGAATA
TGGAAATGAAATAGTAATCATAGATTGTGGTATATCCTTTCCAGATGAGGAGATGTATGGTGTAGATTTGGTTATTCCGG
ATATAACCTACCTTCTAAATAATAAAGATAAAGTAAAAGCTATTTTTCTAACTCATGGACATGAAGACCATATAGGATCT
TTACCTTATATTCTGCAACAATTAAATAGGCCTGTTTATGGTACTGCATTAACCTTAGGTATAGTAGAGAATAAACTAAA
AGAACATAATATGCTTTCAGATTGTGAACTTAATAAGGTGGAAGCAGGAGATATAGTAGAACTTAAAAATTTAAAGATAG
AATTTATAAGAAATACTCATAGTATAGCAGATTCTTGTTCCATAGCTATTCATACGCCGGTGGGAGTTATATTACATACT
GGCGATTTTAAAATAGACTATACACCTATTGATGGATTAGTAATGGATTTTCATCGTATTGCTGAATTAGGCAAAAAAGG
AGTATTACTTCTTATGGCTGATAGTACAAACGTACAGCGTAAGGGGCACACAATTTCCGAGAAATCTATAGGAGAAACCT
TAACTAAAATTTTTTCAAATGCTAAAGGAAGGGTTATTGTAGCAACTTTTGCTTCAAACATACATAGGATGCAGCAAATA
ATAGATGCATCTATAGAGTATGGTAGAAAAGTAGCCTTCAGTGGAAGAAGCATGGAAAATATATCTAAGGTAGCCATGGA
CTTGGGATATCTTCATATACCAGAAACTTATTTAATAACTGTAGATGAAATGAAAAATTATCCTAATGAACAAATAACCA
TAATAACTACTGGAAGTCAAGGAGAACCCATGGCAGCTCTTGCAAGAATAGCTTATTCTAATCATAGAAAAATAGCTATA
GAGCCAAAGGATTTATTTATTATTTCTGCATCACCAATTCCAGGAAATGAAAAACTTATATCTAGAGTAATAAATGAGTT
ATTTAAAAAGGGTGCAGATGTAATATATGAAGCATTAGAAGAGGTACATGTATCTGGTCATGCTTATGAGGAAGAATTAA
AATTAATTCATACATTGGTGCATCCCAAATATTTTATGCCAGTACATGGAGAATACAGGCACCTAAAACGACATGTGGAT
TTAGCCATGGAGTTAGGCATGGAAAGAGAGAACATATTCTCTTTAGAAACAGGGCAAGTTTTAGAAATATCACATGAAGA
AGCAAAAGTATCAGGGAAGGTACGCACAGGATCTATATTTGTAGATGGTATAGGTGTAGGTGATGTAGGGAATATAGTGC
TCAGAGATAGAAGGTATTTAGCGCAGGACGGTATGTTAACCATAGTAGTAACTCTAGAAAAAGAATCCTACAGCGTAATA
GCGGGACCCGATATAATAACAAGGGGATTCATATATGTAAAAGAGTCAGAGGATTTAATAAATGAAGTAAAAGGAATAGT
AAAAAAAGAACTAGAAAATTGTTTAGAAAATAAAATAATTGAATGGTATGTATTAAAATCAAATATTAAAAAATCTGTAG
AAAAATATTTATATGAAAAAACAAAGAGAAGGCCAATAGTTTTACCTATAATTATGGAAATTTAA

Upstream 100 bases:

>100_bases
ATAATCATTAGCTCTTTAGTTAATTTTATTAATTTAAAGAGCAGAATAATATAATAGGTATAGCAAAAAATTTATAAATA
TTGAAAGAGGAGGGTATGAA

Downstream 100 bases:

>100_bases
AATTATAAAAAATAATAAAAAGCTAGAACTTTAATGTTAAATTATAGTTCTAGCTTTTTATTGTATATTAACACATTAAA
ATGATGTAAATATAATAAAA

Product: RNA-metabolising metallo-beta-lactamase

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MSNKDSVKVIPLGGLGEIGKNITAFEYGNEIVIIDCGISFPDEEMYGVDLVIPDITYLLNNKDKVKAIFLTHGHEDHIGS
LPYILQQLNRPVYGTALTLGIVENKLKEHNMLSDCELNKVEAGDIVELKNLKIEFIRNTHSIADSCSIAIHTPVGVILHT
GDFKIDYTPIDGLVMDFHRIAELGKKGVLLLMADSTNVQRKGHTISEKSIGETLTKIFSNAKGRVIVATFASNIHRMQQI
IDASIEYGRKVAFSGRSMENISKVAMDLGYLHIPETYLITVDEMKNYPNEQITIITTGSQGEPMAALARIAYSNHRKIAI
EPKDLFIISASPIPGNEKLISRVINELFKKGADVIYEALEEVHVSGHAYEEELKLIHTLVHPKYFMPVHGEYRHLKRHVD
LAMELGMERENIFSLETGQVLEISHEEAKVSGKVRTGSIFVDGIGVGDVGNIVLRDRRYLAQDGMLTIVVTLEKESYSVI
AGPDIITRGFIYVKESEDLINEVKGIVKKELENCLENKIIEWYVLKSNIKKSVEKYLYEKTKRRPIVLPIIMEI

Sequences:

>Translated_554_residues
MSNKDSVKVIPLGGLGEIGKNITAFEYGNEIVIIDCGISFPDEEMYGVDLVIPDITYLLNNKDKVKAIFLTHGHEDHIGS
LPYILQQLNRPVYGTALTLGIVENKLKEHNMLSDCELNKVEAGDIVELKNLKIEFIRNTHSIADSCSIAIHTPVGVILHT
GDFKIDYTPIDGLVMDFHRIAELGKKGVLLLMADSTNVQRKGHTISEKSIGETLTKIFSNAKGRVIVATFASNIHRMQQI
IDASIEYGRKVAFSGRSMENISKVAMDLGYLHIPETYLITVDEMKNYPNEQITIITTGSQGEPMAALARIAYSNHRKIAI
EPKDLFIISASPIPGNEKLISRVINELFKKGADVIYEALEEVHVSGHAYEEELKLIHTLVHPKYFMPVHGEYRHLKRHVD
LAMELGMERENIFSLETGQVLEISHEEAKVSGKVRTGSIFVDGIGVGDVGNIVLRDRRYLAQDGMLTIVVTLEKESYSVI
AGPDIITRGFIYVKESEDLINEVKGIVKKELENCLENKIIEWYVLKSNIKKSVEKYLYEKTKRRPIVLPIIMEI
>Mature_553_residues
SNKDSVKVIPLGGLGEIGKNITAFEYGNEIVIIDCGISFPDEEMYGVDLVIPDITYLLNNKDKVKAIFLTHGHEDHIGSL
PYILQQLNRPVYGTALTLGIVENKLKEHNMLSDCELNKVEAGDIVELKNLKIEFIRNTHSIADSCSIAIHTPVGVILHTG
DFKIDYTPIDGLVMDFHRIAELGKKGVLLLMADSTNVQRKGHTISEKSIGETLTKIFSNAKGRVIVATFASNIHRMQQII
DASIEYGRKVAFSGRSMENISKVAMDLGYLHIPETYLITVDEMKNYPNEQITIITTGSQGEPMAALARIAYSNHRKIAIE
PKDLFIISASPIPGNEKLISRVINELFKKGADVIYEALEEVHVSGHAYEEELKLIHTLVHPKYFMPVHGEYRHLKRHVDL
AMELGMERENIFSLETGQVLEISHEEAKVSGKVRTGSIFVDGIGVGDVGNIVLRDRRYLAQDGMLTIVVTLEKESYSVIA
GPDIITRGFIYVKESEDLINEVKGIVKKELENCLENKIIEWYVLKSNIKKSVEKYLYEKTKRRPIVLPIIMEI

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 62135; Mature: 62004

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKDSVKVIPLGGLGEIGKNITAFEYGNEIVIIDCGISFPDEEMYGVDLVIPDITYLLN
CCCCCCEEEEEECCCHHHCCCEEEEEECCEEEEEECCCCCCCCCCEEEEEEECCCCEECC
NKDKVKAIFLTHGHEDHIGSLPYILQQLNRPVYGTALTLGIVENKLKEHNMLSDCELNKV
CCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCHHEEEHHHHHHHHHHCCCCCCCCCCCC
EAGDIVELKNLKIEFIRNTHSIADSCSIAIHTPVGVILHTGDFKIDYTPIDGLVMDFHRI
CCCCEEEEECCEEEEECCHHHHCCCCEEEEECCEEEEEECCCEEEEEECCCHHHHHHHHH
AELGKKGVLLLMADSTNVQRKGHTISEKSIGETLTKIFSNAKGRVIVATFASNIHRMQQI
HHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHH
IDASIEYGRKVAFSGRSMENISKVAMDLGYLHIPETYLITVDEMKNYPNEQITIITTGSQ
HHHHHHHCCEEEECCCCHHHHHHHHHHCCEEECCCEEEEEEHHHHCCCCCEEEEEEECCC
GEPMAALARIAYSNHRKIAIEPKDLFIISASPIPGNEKLISRVINELFKKGADVIYEALE
CCHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
EVHVSGHAYEEELKLIHTLVHPKYFMPVHGEYRHLKRHVDLAMELGMERENIFSLETGQV
HHHCCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHCCCCHHHEEEECCCCE
LEISHEEAKVSGKVRTGSIFVDGIGVGDVGNIVLRDRRYLAQDGMLTIVVTLEKESYSVI
EEEECCHHHCCCEEEECEEEEEECCCCCCCCEEECCCHHHHCCCCEEEEEEEECCCEEEE
AGPDIITRGFIYVKESEDLINEVKGIVKKELENCLENKIIEWYVLKSNIKKSVEKYLYEK
ECCCEEECCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHH
TKRRPIVLPIIMEI
HCCCCEEEEEEECC
>Mature Secondary Structure 
SNKDSVKVIPLGGLGEIGKNITAFEYGNEIVIIDCGISFPDEEMYGVDLVIPDITYLLN
CCCCCEEEEEECCCHHHCCCEEEEEECCEEEEEECCCCCCCCCCEEEEEEECCCCEECC
NKDKVKAIFLTHGHEDHIGSLPYILQQLNRPVYGTALTLGIVENKLKEHNMLSDCELNKV
CCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCHHEEEHHHHHHHHHHCCCCCCCCCCCC
EAGDIVELKNLKIEFIRNTHSIADSCSIAIHTPVGVILHTGDFKIDYTPIDGLVMDFHRI
CCCCEEEEECCEEEEECCHHHHCCCCEEEEECCEEEEEECCCEEEEEECCCHHHHHHHHH
AELGKKGVLLLMADSTNVQRKGHTISEKSIGETLTKIFSNAKGRVIVATFASNIHRMQQI
HHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHH
IDASIEYGRKVAFSGRSMENISKVAMDLGYLHIPETYLITVDEMKNYPNEQITIITTGSQ
HHHHHHHCCEEEECCCCHHHHHHHHHHCCEEECCCEEEEEEHHHHCCCCCEEEEEEECCC
GEPMAALARIAYSNHRKIAIEPKDLFIISASPIPGNEKLISRVINELFKKGADVIYEALE
CCHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
EVHVSGHAYEEELKLIHTLVHPKYFMPVHGEYRHLKRHVDLAMELGMERENIFSLETGQV
HHHCCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHCCCCHHHEEEECCCCE
LEISHEEAKVSGKVRTGSIFVDGIGVGDVGNIVLRDRRYLAQDGMLTIVVTLEKESYSVI
EEEECCHHHCCCEEEECEEEEEECCCCCCCCEEECCCHHHHCCCCEEEEEEEECCCEEEE
AGPDIITRGFIYVKESEDLINEVKGIVKKELENCLENKIIEWYVLKSNIKKSVEKYLYEK
ECCCEEECCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHH
TKRRPIVLPIIMEI
HCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]