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The map label for this gene is purF [H]

Identifier: 148359211

GI number: 148359211

Start: 1931991

End: 1933490

Strand: Reverse

Name: purF [H]

Synonym: LPC_1105

Alternate gene names: 148359211

Gene position: 1933490-1931991 (Counterclockwise)

Preceding gene: 148359212

Following gene: 148359210

Centisome position: 54.06

GC content: 39.8

Gene sequence:

>1500_bases
ATGTGTGGCATTGTAGGAATTTACAGTCATGAACCCGTTGCTGCTGAGCTTTATGATAGCTTGATTCATTTACAGCACCG
GGGGCAAGATGCCTCAGGTATTTTAACCGGAGCTGATCGTTTTTACTTCAAACATGGACTTGGACTGGTCAGAGAGTCTA
TTTTCTCTGAAGATATTCTTAAACTGAAGGGGAATATCGGCATTGCTCATGTGCGTTATCCCACGGCCGGAGGTTATAGT
GAAGCGGATGTTCAGCCTCTTTGGATAGGGAGCCCTCGCGGTATAGCTTTGGCTCATAATGGAAATTTAGTGAATTATGG
AGAGTTGGTTGATGATTTATGCGGAAAACAGCATAGGCATTTAAACTCTACTCTTGATTCTGAGGCACTTTTATTACTTC
TGGCAGACTATTTAGCCAAGGGATCTTATTCCGATCATGATGAAGAGCAATTCTTTCAATTACTATGTATGGCAGTTAAT
GAAATACATCAACGAGTTCAGGGTTCCTATTCTGTTGTGAGTTTAGTAATTGGCAAAGGTTTGGTTGCATTTAGGGATCC
GCATGGTATCCGTCCTTTAGTTTGGGGTGAGCGAGTCCATGAAAATGGTAGTAAAGATTATATTTTTGCTTCAGAAACCA
CCCCTTTTTATGCTTTAGGTTTTGAGCCCAAGGGTGATTTGCAGCCCGGTGAAGTTGCTTACGTGAATCAGCAAGGACAA
TTGTACAGAAAAGTGCTTTCATCAAAAAAATTTACCCCTTGTATATTTGAATACGTTTACTTCGCCAGACCAGATGCCAC
ATTAGACGATGTGAGCGTTTATCGAGCCAGATTACGTATGGGGCAAAATTTAGCTAAAAGCTGGAAAAAGAGATTTCCAG
ATACACTACCTGATGTGGTGATTCCAGCCCCTTCAACAGCAAATACTGCCGCATTAGCTTTTGCAAATGAGTTGGGAATA
CGTTATTCCGAAGGTTTATATAAAAATCCTTTTATTGGACGTACTTTTATTATGCCTAATCAGGAGTCGCGATCAAGACA
GGTTCGTTATAAATTAACTCCTCAGAAAACGGAAATTAATAAAAAAAAGGTATTAATCGTTGATGACAGTATCGTAAGGG
GAACTACCTCACGCGAAATAGTCAGAATGATACGAGAATATGGTGCATCAGAAATATATTTTGTATCAACCTGTCCACCA
ATTAAAAACCCTTGTTTCTACGGGATTGATATCCCATCGCGTAAAAAATTGATAGCAGCCCATAAAACGGAAGAGGAAAT
CCGTCAATTTCTAGGGGTGGATAAATTATTATACCAAACTCAGGAAAACCTTATAGAGGCTGTGTCTCGCAGGGGGCAAT
ATGACATGAGCAAGCCTTGTATGGCTTGTATGGATGGTAAATACATTTGTGGACAGATTACTGAAGAAAAAATTAAATTG
TTGGAGAAAAAAAGATTAACCGAGAATGACCAAGAAACAGAGATAGGAGAGATGTCTTGA

Upstream 100 bases:

>100_bases
GAATCGTTTGTGTATGACAGTAATCCTGGGCCAGTAAATGATAGGATTTTGCATAATATACAACGTTGGTTAGGTTAATT
TTTAGCCGGGGGAAAGGCTT

Downstream 100 bases:

>100_bases
TCAATATCCTTATTGTTGGTTCAGGTGCGCGTGAGCATGCAATAGCTACAGCATTGAGTAAATCAGAGAATAAACCAAAG
ATTTATTGCTTTGGAACAAC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MCGIVGIYSHEPVAAELYDSLIHLQHRGQDASGILTGADRFYFKHGLGLVRESIFSEDILKLKGNIGIAHVRYPTAGGYS
EADVQPLWIGSPRGIALAHNGNLVNYGELVDDLCGKQHRHLNSTLDSEALLLLLADYLAKGSYSDHDEEQFFQLLCMAVN
EIHQRVQGSYSVVSLVIGKGLVAFRDPHGIRPLVWGERVHENGSKDYIFASETTPFYALGFEPKGDLQPGEVAYVNQQGQ
LYRKVLSSKKFTPCIFEYVYFARPDATLDDVSVYRARLRMGQNLAKSWKKRFPDTLPDVVIPAPSTANTAALAFANELGI
RYSEGLYKNPFIGRTFIMPNQESRSRQVRYKLTPQKTEINKKKVLIVDDSIVRGTTSREIVRMIREYGASEIYFVSTCPP
IKNPCFYGIDIPSRKKLIAAHKTEEEIRQFLGVDKLLYQTQENLIEAVSRRGQYDMSKPCMACMDGKYICGQITEEKIKL
LEKKRLTENDQETEIGEMS

Sequences:

>Translated_499_residues
MCGIVGIYSHEPVAAELYDSLIHLQHRGQDASGILTGADRFYFKHGLGLVRESIFSEDILKLKGNIGIAHVRYPTAGGYS
EADVQPLWIGSPRGIALAHNGNLVNYGELVDDLCGKQHRHLNSTLDSEALLLLLADYLAKGSYSDHDEEQFFQLLCMAVN
EIHQRVQGSYSVVSLVIGKGLVAFRDPHGIRPLVWGERVHENGSKDYIFASETTPFYALGFEPKGDLQPGEVAYVNQQGQ
LYRKVLSSKKFTPCIFEYVYFARPDATLDDVSVYRARLRMGQNLAKSWKKRFPDTLPDVVIPAPSTANTAALAFANELGI
RYSEGLYKNPFIGRTFIMPNQESRSRQVRYKLTPQKTEINKKKVLIVDDSIVRGTTSREIVRMIREYGASEIYFVSTCPP
IKNPCFYGIDIPSRKKLIAAHKTEEEIRQFLGVDKLLYQTQENLIEAVSRRGQYDMSKPCMACMDGKYICGQITEEKIKL
LEKKRLTENDQETEIGEMS
>Mature_499_residues
MCGIVGIYSHEPVAAELYDSLIHLQHRGQDASGILTGADRFYFKHGLGLVRESIFSEDILKLKGNIGIAHVRYPTAGGYS
EADVQPLWIGSPRGIALAHNGNLVNYGELVDDLCGKQHRHLNSTLDSEALLLLLADYLAKGSYSDHDEEQFFQLLCMAVN
EIHQRVQGSYSVVSLVIGKGLVAFRDPHGIRPLVWGERVHENGSKDYIFASETTPFYALGFEPKGDLQPGEVAYVNQQGQ
LYRKVLSSKKFTPCIFEYVYFARPDATLDDVSVYRARLRMGQNLAKSWKKRFPDTLPDVVIPAPSTANTAALAFANELGI
RYSEGLYKNPFIGRTFIMPNQESRSRQVRYKLTPQKTEINKKKVLIVDDSIVRGTTSREIVRMIREYGASEIYFVSTCPP
IKNPCFYGIDIPSRKKLIAAHKTEEEIRQFLGVDKLLYQTQENLIEAVSRRGQYDMSKPCMACMDGKYICGQITEEKIKL
LEKKRLTENDQETEIGEMS

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=494, Percent_Identity=33.6032388663968, Blast_Score=242, Evalue=7e-64,
Organism=Escherichia coli, GI1788651, Length=488, Percent_Identity=43.2377049180328, Blast_Score=404, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17554892, Length=492, Percent_Identity=35.5691056910569, Blast_Score=250, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6323958, Length=494, Percent_Identity=44.331983805668, Blast_Score=395, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24659598, Length=470, Percent_Identity=32.5531914893617, Blast_Score=238, Evalue=1e-62,
Organism=Drosophila melanogaster, GI28573187, Length=516, Percent_Identity=32.1705426356589, Blast_Score=237, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24659604, Length=470, Percent_Identity=32.5531914893617, Blast_Score=237, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 56269; Mature: 56269

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGIYSHEPVAAELYDSLIHLQHRGQDASGILTGADRFYFKHGLGLVRESIFSEDIL
CCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHH
KLKGNIGIAHVRYPTAGGYSEADVQPLWIGSPRGIALAHNGNLVNYGELVDDLCGKQHRH
HHCCCCEEEEEECCCCCCCCCCCCCEEEECCCCCEEEEECCCEEEHHHHHHHHHCHHHHH
LNSTLDSEALLLLLADYLAKGSYSDHDEEQFFQLLCMAVNEIHQRVQGSYSVVSLVIGKG
HHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
LVAFRDPHGIRPLVWGERVHENGSKDYIFASETTPFYALGFEPKGDLQPGEVAYVNQQGQ
CEEEECCCCCCCCCCCHHHHCCCCCCEEEEECCCCEEEECCCCCCCCCCCCEEEECCCHH
LYRKVLSSKKFTPCIFEYVYFARPDATLDDVSVYRARLRMGQNLAKSWKKRFPDTLPDVV
HHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
IPAPSTANTAALAFANELGIRYSEGLYKNPFIGRTFIMPNQESRSRQVRYKLTPQKTEIN
ECCCCCCCHHHHHHHHHHCCEECCCCCCCCCCCEEEECCCCCCCCCEEEEEECCCHHCCC
KKKVLIVDDSIVRGTTSREIVRMIREYGASEIYFVSTCPPIKNPCFYGIDIPSRKKLIAA
CCEEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEECCCCCCCHHHHH
HKTEEEIRQFLGVDKLLYQTQENLIEAVSRRGQYDMSKPCMACMDGKYICGQITEEKIKL
CCCHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCEEECCCCHHHHHH
LEKKRLTENDQETEIGEMS
HHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MCGIVGIYSHEPVAAELYDSLIHLQHRGQDASGILTGADRFYFKHGLGLVRESIFSEDIL
CCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHH
KLKGNIGIAHVRYPTAGGYSEADVQPLWIGSPRGIALAHNGNLVNYGELVDDLCGKQHRH
HHCCCCEEEEEECCCCCCCCCCCCCEEEECCCCCEEEEECCCEEEHHHHHHHHHCHHHHH
LNSTLDSEALLLLLADYLAKGSYSDHDEEQFFQLLCMAVNEIHQRVQGSYSVVSLVIGKG
HHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
LVAFRDPHGIRPLVWGERVHENGSKDYIFASETTPFYALGFEPKGDLQPGEVAYVNQQGQ
CEEEECCCCCCCCCCCHHHHCCCCCCEEEEECCCCEEEECCCCCCCCCCCCEEEECCCHH
LYRKVLSSKKFTPCIFEYVYFARPDATLDDVSVYRARLRMGQNLAKSWKKRFPDTLPDVV
HHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
IPAPSTANTAALAFANELGIRYSEGLYKNPFIGRTFIMPNQESRSRQVRYKLTPQKTEIN
ECCCCCCCHHHHHHHHHHCCEECCCCCCCCCCCEEEECCCCCCCCCEEEEEECCCHHCCC
KKKVLIVDDSIVRGTTSREIVRMIREYGASEIYFVSTCPPIKNPCFYGIDIPSRKKLIAA
CCEEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEECCCCCCCHHHHH
HKTEEEIRQFLGVDKLLYQTQENLIEAVSRRGQYDMSKPCMACMDGKYICGQITEEKIKL
CCCHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCEEECCCCHHHHHH
LEKKRLTENDQETEIGEMS
HHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7704274 [H]