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The map label for this gene is murD

Identifier: 147674702

GI number: 147674702

Start: 2125461

End: 2126783

Strand: Reverse

Name: murD

Synonym: VC0395_A1981

Alternate gene names: 147674702

Gene position: 2126783-2125461 (Counterclockwise)

Preceding gene: 147674771

Following gene: 147674339

Centisome position: 70.33

GC content: 51.17

Gene sequence:

>1323_bases
ATGGACCGTTGGCAAGGCATACAACATGTGGTCGTCGTGGGGCTCGGGATTACCGGGCTCTCTGTCGTTAATTATCTGCG
AAAATATCATCCGTCGGTAACCGTGCAGGTTATCGATACGCGCGAAATACCACCGGGGCAAGAGCAGCTTTCGTCGGACG
TTGCACTGCACCGTGGCGGGTGGAATCTTGAGTGGTTACTCAATGCCGATTTGGTAGTCACCAATCCAGGCATTGCGTTG
GCGACGCCGGAAATCCAACAAGTGCTGGCCGCTGGAATTCCTGTTGTCGGTGATATTGAGCTCTTTGCATGGCATGTGGA
TACACCCGTTATTGCGATCACTGGCTCGAACGGCAAAAGCACCGTCACTGATTTAAGTGGTGTGTTAGCCAATGCTGCTG
GTGTGAAGGCCGCGGTAGGCGGCAATATTGGGGTTCCGGCACTGGATCTGATTTCTCCAGACGTTGAACTGTATGTGCTT
GAGCTATCAAGCTTCCAATTAGAAACCACCTCTAGTCTAAAACTGAAAGCGGCTGCGTTTCTCAATCTTTCTGAAGATCA
TATGGATCGTTATCAGGGGATGGGAGATTACCGCCAAGCCAAACTGCGCATTTTCGATCATGCCGAAACCGCAGTGGTGA
ATGCCGATGATACTCAAACTTTTCCTGACCACGCGGCGCATCTGCAAGTGGTGACGTTTGGTGTCGAGCAAGCTGCACAA
TTTAGCCTTGCTCAACATCAAGGCCGCGAATACCTTTTTGCGCGCGATGAGGCCGTCATGGCGTGTGCTGAGCTGAGTTT
AGTTGGCCGTCATAACGTGACAAACGTACTTACCGTGTTAGCTTTGCTGGATTCAGCCGGAGTAAACTTCCGTTTGGCTT
TGGATGCACTCAAGTCTTACACGGGTTTGACACACCGCTGCCAAGTGGTTGCCGATAATCACGGCATCAAGTGGGTCAAT
GATTCTAAGGCGACCAATGTTGCGAGTACGCTGGCAGCATTGTCGGGATTAAAAATCGAAGGCCAGCTCTATTTGTTGGT
TGGTGGCGTAGGTAAAGGGGCGGATTTCACGCCGCTCGCCCCCGTATTGGCGACATTGCCTGTGCAATTGTGCTGCTTTG
GTGTGGACGGCCATCAATTTATGCCACTGCACCCTTCGGCTCGGTTTTATGACTCGATGGAGAGCATAATTCGCTCAATT
CGCCCGCAATTAAAAAGTGGGGATATGGTGCTGCTCTCTCCCGCTTGTGCGAGCTTTGATCAGTTCAAAAACTTTATGGC
GCGTGGGGATATTTTTGCCCAACTCGCTCGGCAATATGCATAA

Upstream 100 bases:

>100_bases
CACGTGTGATCGTGCGTTTTTGGGTCATCTCTATCGTTTTAGTATTGATTGGCTTAGCGACACTCAAAGTTCGTTAAGTG
GATTAAGGATGTCAATTTCA

Downstream 100 bases:

>100_bases
GCGTTAGGTAAGTCACTTGTTTTTATCCGGATCTTTCACAAAACTGAGCCACTGGTTGAGAACCTCCTCACCAGAGGCGC
TGTTTGACCGTCAATTGGTC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MDRWQGIQHVVVVGLGITGLSVVNYLRKYHPSVTVQVIDTREIPPGQEQLSSDVALHRGGWNLEWLLNADLVVTNPGIAL
ATPEIQQVLAAGIPVVGDIELFAWHVDTPVIAITGSNGKSTVTDLSGVLANAAGVKAAVGGNIGVPALDLISPDVELYVL
ELSSFQLETTSSLKLKAAAFLNLSEDHMDRYQGMGDYRQAKLRIFDHAETAVVNADDTQTFPDHAAHLQVVTFGVEQAAQ
FSLAQHQGREYLFARDEAVMACAELSLVGRHNVTNVLTVLALLDSAGVNFRLALDALKSYTGLTHRCQVVADNHGIKWVN
DSKATNVASTLAALSGLKIEGQLYLLVGGVGKGADFTPLAPVLATLPVQLCCFGVDGHQFMPLHPSARFYDSMESIIRSI
RPQLKSGDMVLLSPACASFDQFKNFMARGDIFAQLARQYA

Sequences:

>Translated_440_residues
MDRWQGIQHVVVVGLGITGLSVVNYLRKYHPSVTVQVIDTREIPPGQEQLSSDVALHRGGWNLEWLLNADLVVTNPGIAL
ATPEIQQVLAAGIPVVGDIELFAWHVDTPVIAITGSNGKSTVTDLSGVLANAAGVKAAVGGNIGVPALDLISPDVELYVL
ELSSFQLETTSSLKLKAAAFLNLSEDHMDRYQGMGDYRQAKLRIFDHAETAVVNADDTQTFPDHAAHLQVVTFGVEQAAQ
FSLAQHQGREYLFARDEAVMACAELSLVGRHNVTNVLTVLALLDSAGVNFRLALDALKSYTGLTHRCQVVADNHGIKWVN
DSKATNVASTLAALSGLKIEGQLYLLVGGVGKGADFTPLAPVLATLPVQLCCFGVDGHQFMPLHPSARFYDSMESIIRSI
RPQLKSGDMVLLSPACASFDQFKNFMARGDIFAQLARQYA
>Mature_440_residues
MDRWQGIQHVVVVGLGITGLSVVNYLRKYHPSVTVQVIDTREIPPGQEQLSSDVALHRGGWNLEWLLNADLVVTNPGIAL
ATPEIQQVLAAGIPVVGDIELFAWHVDTPVIAITGSNGKSTVTDLSGVLANAAGVKAAVGGNIGVPALDLISPDVELYVL
ELSSFQLETTSSLKLKAAAFLNLSEDHMDRYQGMGDYRQAKLRIFDHAETAVVNADDTQTFPDHAAHLQVVTFGVEQAAQ
FSLAQHQGREYLFARDEAVMACAELSLVGRHNVTNVLTVLALLDSAGVNFRLALDALKSYTGLTHRCQVVADNHGIKWVN
DSKATNVASTLAALSGLKIEGQLYLLVGGVGKGADFTPLAPVLATLPVQLCCFGVDGHQFMPLHPSARFYDSMESIIRSI
RPQLKSGDMVLLSPACASFDQFKNFMARGDIFAQLARQYA

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=440, Percent_Identity=52.9545454545455, Blast_Score=422, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_VIBC3 (A5F5N1)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F5N1
- SMR:   A5F5N1
- STRING:   A5F5N1
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A1981
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   PRK04663
- BioCyc:   VCHO345073:VC0395_A1981-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 47380; Mature: 47380

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRWQGIQHVVVVGLGITGLSVVNYLRKYHPSVTVQVIDTREIPPGQEQLSSDVALHRGG
CCCHHHCCEEEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHCCCCEEECCC
WNLEWLLNADLVVTNPGIALATPEIQQVLAAGIPVVGDIELFAWHVDTPVIAITGSNGKS
CCEEEEEECCEEEECCCCEEECHHHHHHHHCCCCEEECEEEEEEEECCCEEEEECCCCCC
TVTDLSGVLANAAGVKAAVGGNIGVPALDLISPDVELYVLELSSFQLETTSSLKLKAAAF
HHHHHHHHHHCCCCCEEECCCCCCCCCHHHCCCCCEEEEEEECCEEEECCCCCEEEEEEE
LNLSEDHMDRYQGMGDYRQAKLRIFDHAETAVVNADDTQTFPDHAAHLQVVTFGVEQAAQ
ECCCHHHHHHHCCCCCHHHEEEEEEECCCEEEEECCCCCCCCCCCCEEEEEEECHHHHHH
FSLAQHQGREYLFARDEAVMACAELSLVGRHNVTNVLTVLALLDSAGVNFRLALDALKSY
HHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHH
TGLTHRCQVVADNHGIKWVNDSKATNVASTLAALSGLKIEGQLYLLVGGVGKGADFTPLA
CCCCEEEEEEECCCCEEEECCCCHHHHHHHHHHHCCCEECCEEEEEEECCCCCCCCCHHH
PVLATLPVQLCCFGVDGHQFMPLHPSARFYDSMESIIRSIRPQLKSGDMVLLSPACASFD
HHHHHCCEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHH
QFKNFMARGDIFAQLARQYA
HHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MDRWQGIQHVVVVGLGITGLSVVNYLRKYHPSVTVQVIDTREIPPGQEQLSSDVALHRGG
CCCHHHCCEEEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHCCCCEEECCC
WNLEWLLNADLVVTNPGIALATPEIQQVLAAGIPVVGDIELFAWHVDTPVIAITGSNGKS
CCEEEEEECCEEEECCCCEEECHHHHHHHHCCCCEEECEEEEEEEECCCEEEEECCCCCC
TVTDLSGVLANAAGVKAAVGGNIGVPALDLISPDVELYVLELSSFQLETTSSLKLKAAAF
HHHHHHHHHHCCCCCEEECCCCCCCCCHHHCCCCCEEEEEEECCEEEECCCCCEEEEEEE
LNLSEDHMDRYQGMGDYRQAKLRIFDHAETAVVNADDTQTFPDHAAHLQVVTFGVEQAAQ
ECCCHHHHHHHCCCCCHHHEEEEEEECCCEEEEECCCCCCCCCCCCEEEEEEECHHHHHH
FSLAQHQGREYLFARDEAVMACAELSLVGRHNVTNVLTVLALLDSAGVNFRLALDALKSY
HHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHH
TGLTHRCQVVADNHGIKWVNDSKATNVASTLAALSGLKIEGQLYLLVGGVGKGADFTPLA
CCCCEEEEEEECCCCEEEECCCCHHHHHHHHHHHCCCEECCEEEEEEECCCCCCCCCHHH
PVLATLPVQLCCFGVDGHQFMPLHPSARFYDSMESIIRSIRPQLKSGDMVLLSPACASFD
HHHHHCCEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHH
QFKNFMARGDIFAQLARQYA
HHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA