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The map label for this gene is yqhT [H]

Identifier: 146343379

GI number: 146343379

Start: 6856859

End: 6858175

Strand: Reverse

Name: yqhT [H]

Synonym: BRADO6608

Alternate gene names: 146343379

Gene position: 6858175-6856859 (Counterclockwise)

Preceding gene: 146343381

Following gene: 146343378

Centisome position: 91.97

GC content: 64.24

Gene sequence:

>1317_bases
ATGGGAAACGTCCACATGACAGCCAGCGCGCTGAAGCCGCATCTGGTCAGCCCCGAGCATCTCGATCCGCATTGGCGCTG
GAGCCGGGCGATTCCCTCGCACGGCCATGTCTCGGTCGATTTCGAGCGCCGCGTCGATTTCGATCGTCTGCGGCGATACC
GTCTGGCGCGGGCGCGCCAGGCGCTGAAGGCCTCGGGCTGTGGCGCGCTGCTGCTGTTCGACGTCAACAACATCCGCTAC
GTCTCCGGCACCAAGATCGGCGAATGGGAGCGCGACAAGCTCTGTCGCTTCGCGCTCCTGGCCGGCGACGGCGAGCCGAT
CGTGTGGGATTTCGGTTCGGCCGCCGTGCACCACCGCATCTTCTGCGACTGGCTGGCGCCTGAGAACTGCCGCGCCGGCA
TGCTCGGCATGCGCGGCACGGTGCCGCCGGCCGTCGGCCTGATGAAGGCTCATGCCGAAGAAGTCATGAGCCTGCTGCGC
GCGGCCGGCGTCGCCGACATGCCGGTCGGTGTCGACCTTGCTGAAACCGCGATGTTCTTCGAGCTGCAAAAGGCCGGCAT
GAAGGTCGTCGACGGCCAGCAGGTCATGCTCGACGCGCGCGAGATCAAGAACATCGACGAGATCATGCTGCTCAACCAGG
CCGCGGCAATGGTCGACGGCGTCTATCATTCGATTTACGAGAATTTGAAGCCCGGCGTCCGCGAGAACGACATCGTCGCG
CTCGCCAACAAGATGCTCTACGAGATGGGCTCCGACGACGTCGAGGCGATCAACGCGATATCCGGCGAGCGCTGCAATCC
GCATCCGCACAATTTCACCGACCGCATCCTGCGTCCGGGCGACCAGGCGTTCTTCGACATCCTGCAGTCCTATCAGGGCT
ACCGCACCTGCTATTACCGCACCTTCAATGTCGGCCGAGCCACGCCGGCCCAGCACGATGCCTACAAGCGCTGCCGCGAG
TGGCTCGACAACGCGATCGCGTTGATCAAGCCGGGCGTGTCGACCGATTCCGTCGCCAAGGTGTGGCCGGCGGCGGAGGA
GTTCGGATTCCCCTCGGAGATGGCGGCGTTCGGCCTGCAATTTGGCCACGGTCTTGGTCTCGCTCTGCATGAGCGCCCGA
TCATTTCGCGCCTGGTCTCGCTGGACAATCCGATGGAGATCAAGACCGGCATGGTATTCGCGCTGGAGACTTATTGCCCA
GCCACTGACGGCTTCTCCGCGGCGCGCATCGAGGAGGAGGTCGTGGTCACCGACAAGGGCTGCCAGGTGATCACGCTGTT
CCCCGCGGAAGAGCTGCCGATCGCCAATCGTTACTGA

Upstream 100 bases:

>100_bases
CCTCTGATGTCGCAGCGCAGCGACGTTTCCCTCTGACTTACCTTGACACTCTTAACGTGCATGCAATAAATCCGGCTCTG
CAATCGATTGCAATAACTCA

Downstream 100 bases:

>100_bases
GCGAGATAATGCTACGAGCAAGACTGCCTTTCTAAATTTCTCGTCATTGCGAGCGAAGCGAAGCAATCCAGGGCCGAGCA
AGGACTCTGGGTTGCTTCGT

Product: putative aminopeptidase proline or methionine dipeptidase

Products: N-terminal aminoacid; peptide preferentially methionine [C]

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MGNVHMTASALKPHLVSPEHLDPHWRWSRAIPSHGHVSVDFERRVDFDRLRRYRLARARQALKASGCGALLLFDVNNIRY
VSGTKIGEWERDKLCRFALLAGDGEPIVWDFGSAAVHHRIFCDWLAPENCRAGMLGMRGTVPPAVGLMKAHAEEVMSLLR
AAGVADMPVGVDLAETAMFFELQKAGMKVVDGQQVMLDAREIKNIDEIMLLNQAAAMVDGVYHSIYENLKPGVRENDIVA
LANKMLYEMGSDDVEAINAISGERCNPHPHNFTDRILRPGDQAFFDILQSYQGYRTCYYRTFNVGRATPAQHDAYKRCRE
WLDNAIALIKPGVSTDSVAKVWPAAEEFGFPSEMAAFGLQFGHGLGLALHERPIISRLVSLDNPMEIKTGMVFALETYCP
ATDGFSAARIEEEVVVTDKGCQVITLFPAEELPIANRY

Sequences:

>Translated_438_residues
MGNVHMTASALKPHLVSPEHLDPHWRWSRAIPSHGHVSVDFERRVDFDRLRRYRLARARQALKASGCGALLLFDVNNIRY
VSGTKIGEWERDKLCRFALLAGDGEPIVWDFGSAAVHHRIFCDWLAPENCRAGMLGMRGTVPPAVGLMKAHAEEVMSLLR
AAGVADMPVGVDLAETAMFFELQKAGMKVVDGQQVMLDAREIKNIDEIMLLNQAAAMVDGVYHSIYENLKPGVRENDIVA
LANKMLYEMGSDDVEAINAISGERCNPHPHNFTDRILRPGDQAFFDILQSYQGYRTCYYRTFNVGRATPAQHDAYKRCRE
WLDNAIALIKPGVSTDSVAKVWPAAEEFGFPSEMAAFGLQFGHGLGLALHERPIISRLVSLDNPMEIKTGMVFALETYCP
ATDGFSAARIEEEVVVTDKGCQVITLFPAEELPIANRY
>Mature_437_residues
GNVHMTASALKPHLVSPEHLDPHWRWSRAIPSHGHVSVDFERRVDFDRLRRYRLARARQALKASGCGALLLFDVNNIRYV
SGTKIGEWERDKLCRFALLAGDGEPIVWDFGSAAVHHRIFCDWLAPENCRAGMLGMRGTVPPAVGLMKAHAEEVMSLLRA
AGVADMPVGVDLAETAMFFELQKAGMKVVDGQQVMLDAREIKNIDEIMLLNQAAAMVDGVYHSIYENLKPGVRENDIVAL
ANKMLYEMGSDDVEAINAISGERCNPHPHNFTDRILRPGDQAFFDILQSYQGYRTCYYRTFNVGRATPAQHDAYKRCREW
LDNAIALIKPGVSTDSVAKVWPAAEEFGFPSEMAAFGLQFGHGLGLALHERPIISRLVSLDNPMEIKTGMVFALETYCPA
TDGFSAARIEEEVVVTDKGCQVITLFPAEELPIANRY

Specific function: Removes The Amino-Terminal Methionine From Nascent Proteins. [C]

COG id: COG0006

COG function: function code E; Xaa-Pro aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24B family [H]

Homologues:

Organism=Escherichia coli, GI1786364, Length=263, Percent_Identity=23.574144486692, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000587
- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR001131 [H]

Pfam domain/function: PF01321 Creatinase_N; PF00557 Peptidase_M24 [H]

EC number: 3.4.11.18 [C]

Molecular weight: Translated: 48776; Mature: 48645

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNVHMTASALKPHLVSPEHLDPHWRWSRAIPSHGHVSVDFERRVDFDRLRRYRLARARQ
CCCEEEEHHHCCCCCCCCCCCCCCCHHHCCCCCCCCEEEEHHHCCCHHHHHHHHHHHHHH
ALKASGCGALLLFDVNNIRYVSGTKIGEWERDKLCRFALLAGDGEPIVWDFGSAAVHHRI
HHHHCCCCEEEEEECCCEEEECCCCCCCCCHHHHHEEEEEECCCCEEEEECCCHHHHHHE
FCDWLAPENCRAGMLGMRGTVPPAVGLMKAHAEEVMSLLRAAGVADMPVGVDLAETAMFF
EEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
ELQKAGMKVVDGQQVMLDAREIKNIDEIMLLNQAAAMVDGVYHSIYENLKPGVRENDIVA
HHHHCCCEEECCCHHEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LANKMLYEMGSDDVEAINAISGERCNPHPHNFTDRILRPGDQAFFDILQSYQGYRTCYYR
HHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHCCCCEEEEEE
TFNVGRATPAQHDAYKRCREWLDNAIALIKPGVSTDSVAKVWPAAEEFGFPSEMAAFGLQ
EEECCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHCCCHHHCCCCHHHHHHHHH
FGHGLGLALHERPIISRLVSLDNPMEIKTGMVFALETYCPATDGFSAARIEEEVVVTDKG
HCCCCCEEECCCHHHHHHHHCCCCCEECCCEEEEEEECCCCCCCCCHHHCCCEEEEECCC
CQVITLFPAEELPIANRY
CEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
GNVHMTASALKPHLVSPEHLDPHWRWSRAIPSHGHVSVDFERRVDFDRLRRYRLARARQ
CCEEEEHHHCCCCCCCCCCCCCCCHHHCCCCCCCCEEEEHHHCCCHHHHHHHHHHHHHH
ALKASGCGALLLFDVNNIRYVSGTKIGEWERDKLCRFALLAGDGEPIVWDFGSAAVHHRI
HHHHCCCCEEEEEECCCEEEECCCCCCCCCHHHHHEEEEEECCCCEEEEECCCHHHHHHE
FCDWLAPENCRAGMLGMRGTVPPAVGLMKAHAEEVMSLLRAAGVADMPVGVDLAETAMFF
EEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
ELQKAGMKVVDGQQVMLDAREIKNIDEIMLLNQAAAMVDGVYHSIYENLKPGVRENDIVA
HHHHCCCEEECCCHHEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LANKMLYEMGSDDVEAINAISGERCNPHPHNFTDRILRPGDQAFFDILQSYQGYRTCYYR
HHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHCCCCEEEEEE
TFNVGRATPAQHDAYKRCREWLDNAIALIKPGVSTDSVAKVWPAAEEFGFPSEMAAFGLQ
EEECCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHCCCHHHCCCCHHHHHHHHH
FGHGLGLALHERPIISRLVSLDNPMEIKTGMVFALETYCPATDGFSAARIEEEVVVTDKG
HCCCCCEEECCCHHHHHHHHCCCCCEECCCEEEEEEECCCCCCCCCHHHCCCEEEEECCC
CQVITLFPAEELPIANRY
CEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Co2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Peptide; H2O [C]

Specific reaction: peptide + H2O = N-terminal aminoacid + peptide [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: EDTA [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]