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The map label for this gene is glxR [C]

Identifier: 146343378

GI number: 146343378

Start: 6855739

End: 6856704

Strand: Reverse

Name: glxR [C]

Synonym: BRADO6607

Alternate gene names: 146343378

Gene position: 6856704-6855739 (Counterclockwise)

Preceding gene: 146343379

Following gene: 146343374

Centisome position: 91.95

GC content: 64.91

Gene sequence:

>966_bases
ATGACCGCGACGCATTCGCTCGGCTGGATCGGCATGGGGCGCATGGGCTATCCGATGGCCGAGCGGCTGCTGAAAGCCGG
ACACAAGGTGTCGATCTTCAACCGCACCCGGGCCAAGGCCGAGCCGCTGGCGACGAAGGGCGGCATCATCGTCGACCATC
CGAGCGATCTCGCAGGATGCGACATCGTCTTCTCGATGGTGTCGACGGCCAAGGATCTCGAGCAGGTCTATTTCGGCGAC
CATGGACTCGTCGCGTCGGGCTCGGGTCCGCGTCCTCAGATCCTGGTCGATTGCTCCTCCATCGGTGTCGGGCAGTCCGA
GGCGATCGACGCCAAGCTGAGACAGCTCGACTGCGCGTTCGTGCGCGCCCCGGTGTCCGGCAACGGCAAATGCGTCAAGG
CCGGCAAGCTGTCATCGGTCGTGTCCGGACCCAAAGCCGCGTTCGATGCCATCGCACCATATCTCGCCAAGATCGCCGTC
TCCGGCGTGTCCTACGTCGGCGAGGGCGAGCTCGCGCGCATCTGCAAGATTGCGCACAACGTCTTTCTCGGCGTCGTGAT
CCAGAATCTGGCCGAAATCACCATCCTGGCGCAGAAGGCCGGCGTGCCGCGCCATGCCTTCCTCGACTTCATGAATGCCA
GCGTCATGGGCTCGACCTTCACCAAGTACAAGAGCAACGCGCTGGTCAATCTCGACTGGACCACGACCTTCACGCCGACC
CTTCTGCGCAAGGATCTCGATCTCGGCCTGTCGGCGGCGCGCCAACTCGACGTCGCGATGCCGGTGACGGCGGCAACGCG
CGAGGCGCTGCAGGCCCATGTCGGCGCGGCGTCGCTGCAGAGCGATCCCGCCGCCTACCTGGAAAAGGATTTTGCGGCGC
TGCTGGAGACGGTGGCGCTGGCTGCCGGCCTCAAGCTGCAGCCGGAAAATGTCCCGATGCCGACGGGCTTGGAAACCGAG
GGGTAG

Upstream 100 bases:

>100_bases
GAAGCGAAGCAATCCAGGGCCGAGCAAGGACTCTGGGTTGCTTCGTCGCTTCGCTCCTCGCAATGACGACTGCCGTGACC
AGTATGACGAGGAGACGACA

Downstream 100 bases:

>100_bases
GGCGGCCTCTTGGCCAGGGACGTCATTCCGGGGCAAGGCCGCAGGCCTTGAACCCGGAATCTCGAGCTTGTCTTGCGTCC
GCTCCAATAATCTCGGGAGT

Product: putative 3-hydroxyisobutyrate dehydrogenase

Products: (R)-Glycerate; Nicotinamide adenine dinucleotide [C]

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MTATHSLGWIGMGRMGYPMAERLLKAGHKVSIFNRTRAKAEPLATKGGIIVDHPSDLAGCDIVFSMVSTAKDLEQVYFGD
HGLVASGSGPRPQILVDCSSIGVGQSEAIDAKLRQLDCAFVRAPVSGNGKCVKAGKLSSVVSGPKAAFDAIAPYLAKIAV
SGVSYVGEGELARICKIAHNVFLGVVIQNLAEITILAQKAGVPRHAFLDFMNASVMGSTFTKYKSNALVNLDWTTTFTPT
LLRKDLDLGLSAARQLDVAMPVTAATREALQAHVGAASLQSDPAAYLEKDFAALLETVALAAGLKLQPENVPMPTGLETE
G

Sequences:

>Translated_321_residues
MTATHSLGWIGMGRMGYPMAERLLKAGHKVSIFNRTRAKAEPLATKGGIIVDHPSDLAGCDIVFSMVSTAKDLEQVYFGD
HGLVASGSGPRPQILVDCSSIGVGQSEAIDAKLRQLDCAFVRAPVSGNGKCVKAGKLSSVVSGPKAAFDAIAPYLAKIAV
SGVSYVGEGELARICKIAHNVFLGVVIQNLAEITILAQKAGVPRHAFLDFMNASVMGSTFTKYKSNALVNLDWTTTFTPT
LLRKDLDLGLSAARQLDVAMPVTAATREALQAHVGAASLQSDPAAYLEKDFAALLETVALAAGLKLQPENVPMPTGLETE
G
>Mature_320_residues
TATHSLGWIGMGRMGYPMAERLLKAGHKVSIFNRTRAKAEPLATKGGIIVDHPSDLAGCDIVFSMVSTAKDLEQVYFGDH
GLVASGSGPRPQILVDCSSIGVGQSEAIDAKLRQLDCAFVRAPVSGNGKCVKAGKLSSVVSGPKAAFDAIAPYLAKIAVS
GVSYVGEGELARICKIAHNVFLGVVIQNLAEITILAQKAGVPRHAFLDFMNASVMGSTFTKYKSNALVNLDWTTTFTPTL
LRKDLDLGLSAARQLDVAMPVTAATREALQAHVGAASLQSDPAAYLEKDFAALLETVALAAGLKLQPENVPMPTGLETEG

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=268, Percent_Identity=26.1194029850746, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI23308751, Length=259, Percent_Identity=26.6409266409266, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1786719, Length=283, Percent_Identity=26.8551236749117, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI145693186, Length=268, Percent_Identity=29.8507462686567, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1790315, Length=302, Percent_Identity=25.4966887417219, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789092, Length=284, Percent_Identity=25.7042253521127, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28574115, Length=272, Percent_Identity=26.1029411764706, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24655230, Length=280, Percent_Identity=26.7857142857143, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI19922568, Length=280, Percent_Identity=26.7857142857143, Blast_Score=91, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.60 [C]

Molecular weight: Translated: 33736; Mature: 33605

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATHSLGWIGMGRMGYPMAERLLKAGHKVSIFNRTRAKAEPLATKGGIIVDHPSDLAGC
CCCCCCCCCEECCCCCCHHHHHHHHCCCEEEEECCCHHHCCCCCCCCCEEEECCCCHHHH
DIVFSMVSTAKDLEQVYFGDHGLVASGSGPRPQILVDCSSIGVGQSEAIDAKLRQLDCAF
HHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHE
VRAPVSGNGKCVKAGKLSSVVSGPKAAFDAIAPYLAKIAVSGVSYVGEGELARICKIAHN
EECCCCCCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
VFLGVVIQNLAEITILAQKAGVPRHAFLDFMNASVMGSTFTKYKSNALVNLDWTTTFTPT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHCCCEEEEECCCCCCCHH
LLRKDLDLGLSAARQLDVAMPVTAATREALQAHVGAASLQSDPAAYLEKDFAALLETVAL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHCCCCHHHHHHHHHHHHHHHHHH
AAGLKLQPENVPMPTGLETEG
HHCCEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
TATHSLGWIGMGRMGYPMAERLLKAGHKVSIFNRTRAKAEPLATKGGIIVDHPSDLAGC
CCCCCCCCEECCCCCCHHHHHHHHCCCEEEEECCCHHHCCCCCCCCCEEEECCCCHHHH
DIVFSMVSTAKDLEQVYFGDHGLVASGSGPRPQILVDCSSIGVGQSEAIDAKLRQLDCAF
HHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHE
VRAPVSGNGKCVKAGKLSSVVSGPKAAFDAIAPYLAKIAVSGVSYVGEGELARICKIAHN
EECCCCCCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
VFLGVVIQNLAEITILAQKAGVPRHAFLDFMNASVMGSTFTKYKSNALVNLDWTTTFTPT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHCCCEEEEECCCCCCCHH
LLRKDLDLGLSAARQLDVAMPVTAATREALQAHVGAASLQSDPAAYLEKDFAALLETVAL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHCCCCHHHHHHHHHHHHHHHHHH
AAGLKLQPENVPMPTGLETEG
HHCCEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Hydroxy-3-oxopropanoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Specific reaction: 2-Hydroxy-3-oxopropanoate + H+ + Nicotinamide adenine dinucleotide - reduced --> (R)-Glycerate + Nicotinamide adenine dinucleotide [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA