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The map label for this gene is nylB [H]

Identifier: 146343303

GI number: 146343303

Start: 6778663

End: 6779853

Strand: Reverse

Name: nylB [H]

Synonym: BRADO6526

Alternate gene names: 146343303

Gene position: 6779853-6778663 (Counterclockwise)

Preceding gene: 146343311

Following gene: 146343302

Centisome position: 90.92

GC content: 67.93

Gene sequence:

>1191_bases
ATGCGACCGACCGACATCATGCGCGGCAGCCCTCCGCCGCCGGAGGCGCAGGTCACCAAAGCCAATTGGCGAACCTATCC
CGCCATCCGCTGGGGCTTCACTCATGCGCGTGAATTGCTGCCCACCGCAGCGATCCGCCGCGCGTCGCAGCCGACGCCGC
TGGTGGAGGCGCAACGCGAGCTGACGAGCCTCGCGTTCTCGACGCCCGACGGTCAGCGCACCACGGTGGCCGAGACCTTG
CAGGCCACCGATGCCGATGCGCTGATCGTGATGCATCGCGGCGTCATCCTCACCGAATGGTACGGCGAGGGCATGAGCGA
GACCACGCCGCACATGCTGTGCTCGGTGAGCAAGTCGATCTGCGGCACGCTCGGCGGCGTGCTGGCGGCGCGCGGCTTGA
TCGATCCGGGCGACAAGGTCACCGCCTATCTGCCGGAGCTCGCGGGCACCGCCTATGACGGCTGCACTCTCCGGCATCTG
CTCGACATGCAGGTCGGCATCCGCTTCGAGGAGGATTACGACGATCCCAATGGCGACGTGGCGCGTTACCGCCATTCGTC
GGGCTGGGACATCGCCCCTCCGGGTGTGCCGGCCGGCGATCAGCGCGGCTTCCTGACGACGCTGCGCCCCAGCGGCAGGC
CGCATGGCAAGCTGTTTCACTACGTGTCGCCGAACACCGATGTCTGTGGCTGGGTCTATGAGCGGGCCTGCGGCGCGCCC
TATGCGAAGATCCTGTCGGACTATCTGTGGCAGCCGATGGGCGCGGCCGAGGATGCCTCGATCACCCTGGACTCGATGGG
AGCAGGGCGCTTCGCCGGCGGCATCTCGGCGACGGCGCGTGATCTCGCGCGCTTCGGCGAGATGATCCGCTGCCGCGGCC
TTGTGCAGGGCCGGCAGGTCGTGCCGGGCGCGTGGATCGACGACATCCATGATGCCGGCGACCGGCAGGCCTGGGCCAAC
GGCGATCTCGCCTTCGTGTTTCCCGACGGGTGCTACCGCAGCAACTGGTACACGGTCGACGTCAAGCGGCGGGACCTCGC
CGCGGTCGGCATCCACGGCCAATGGATCTATGTCGATATCGCCAGCGACTCCGTGATCGTCAAGCTCGCCACCCAGGGCA
AGGCGATGGACATCGCCCAGGACCATCGCTGGCTCGCCGGCTTCCGCGCCATCACCGCGCATCTCTCCTAA

Upstream 100 bases:

>100_bases
CCGGAAGCATACCCACGGATGATCTTGCCCGATTTCGATAACGCTGTCCTGCGCCTGATTGGCAGTCTCGGCTCATTTCC
ACGTGCCGAGCGAGGACGCG

Downstream 100 bases:

>100_bases
TCGAACCGATGGAGTCCAACGTGCAGGACATCACCGCCGCCGCGACCGCCGTGGTTCATCCGCTCGACCCTTTGACATCG
GACGAGATCGCGGCCGCCTG

Product: putative beta-lactamase family protein 6-aminohexanoate-dimer hydrolase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EII [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MRPTDIMRGSPPPPEAQVTKANWRTYPAIRWGFTHARELLPTAAIRRASQPTPLVEAQRELTSLAFSTPDGQRTTVAETL
QATDADALIVMHRGVILTEWYGEGMSETTPHMLCSVSKSICGTLGGVLAARGLIDPGDKVTAYLPELAGTAYDGCTLRHL
LDMQVGIRFEEDYDDPNGDVARYRHSSGWDIAPPGVPAGDQRGFLTTLRPSGRPHGKLFHYVSPNTDVCGWVYERACGAP
YAKILSDYLWQPMGAAEDASITLDSMGAGRFAGGISATARDLARFGEMIRCRGLVQGRQVVPGAWIDDIHDAGDRQAWAN
GDLAFVFPDGCYRSNWYTVDVKRRDLAAVGIHGQWIYVDIASDSVIVKLATQGKAMDIAQDHRWLAGFRAITAHLS

Sequences:

>Translated_396_residues
MRPTDIMRGSPPPPEAQVTKANWRTYPAIRWGFTHARELLPTAAIRRASQPTPLVEAQRELTSLAFSTPDGQRTTVAETL
QATDADALIVMHRGVILTEWYGEGMSETTPHMLCSVSKSICGTLGGVLAARGLIDPGDKVTAYLPELAGTAYDGCTLRHL
LDMQVGIRFEEDYDDPNGDVARYRHSSGWDIAPPGVPAGDQRGFLTTLRPSGRPHGKLFHYVSPNTDVCGWVYERACGAP
YAKILSDYLWQPMGAAEDASITLDSMGAGRFAGGISATARDLARFGEMIRCRGLVQGRQVVPGAWIDDIHDAGDRQAWAN
GDLAFVFPDGCYRSNWYTVDVKRRDLAAVGIHGQWIYVDIASDSVIVKLATQGKAMDIAQDHRWLAGFRAITAHLS
>Mature_396_residues
MRPTDIMRGSPPPPEAQVTKANWRTYPAIRWGFTHARELLPTAAIRRASQPTPLVEAQRELTSLAFSTPDGQRTTVAETL
QATDADALIVMHRGVILTEWYGEGMSETTPHMLCSVSKSICGTLGGVLAARGLIDPGDKVTAYLPELAGTAYDGCTLRHL
LDMQVGIRFEEDYDDPNGDVARYRHSSGWDIAPPGVPAGDQRGFLTTLRPSGRPHGKLFHYVSPNTDVCGWVYERACGAP
YAKILSDYLWQPMGAAEDASITLDSMGAGRFAGGISATARDLARFGEMIRCRGLVQGRQVVPGAWIDDIHDAGDRQAWAN
GDLAFVFPDGCYRSNWYTVDVKRRDLAAVGIHGQWIYVDIASDSVIVKLATQGKAMDIAQDHRWLAGFRAITAHLS

Specific function: This Protein Is A Serine Beta-Lactamase With A Substrate Specificity For Cephalosporins. [C]

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: =3.5.1.46 [H]

Molecular weight: Translated: 43256; Mature: 43256

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPTDIMRGSPPPPEAQVTKANWRTYPAIRWGFTHARELLPTAAIRRASQPTPLVEAQRE
CCCCCCCCCCCCCCCCEEEECCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LTSLAFSTPDGQRTTVAETLQATDADALIVMHRGVILTEWYGEGMSETTPHMLCSVSKSI
HHHHHCCCCCCCCHHHHHHHHHCCCCEEEEEECCEEEEEHHCCCCCCCCHHHHHHHHHHH
CGTLGGVLAARGLIDPGDKVTAYLPELAGTAYDGCTLRHLLDMQVGIRFEEDYDDPNGDV
HHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCEEEECCCCCCCCCH
ARYRHSSGWDIAPPGVPAGDQRGFLTTLRPSGRPHGKLFHYVSPNTDVCGWVYERACGAP
HHEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHCCCC
YAKILSDYLWQPMGAAEDASITLDSMGAGRFAGGISATARDLARFGEMIRCRGLVQGRQV
HHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEE
VPGAWIDDIHDAGDRQAWANGDLAFVFPDGCYRSNWYTVDVKRRDLAAVGIHGQWIYVDI
CCCCCHHHHHCCCCCCCCCCCCEEEECCCCCCCCCEEEEEEECCCEEEEEECCEEEEEEE
ASDSVIVKLATQGKAMDIAQDHRWLAGFRAITAHLS
CCCCEEEEEECCCCEECHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRPTDIMRGSPPPPEAQVTKANWRTYPAIRWGFTHARELLPTAAIRRASQPTPLVEAQRE
CCCCCCCCCCCCCCCCEEEECCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LTSLAFSTPDGQRTTVAETLQATDADALIVMHRGVILTEWYGEGMSETTPHMLCSVSKSI
HHHHHCCCCCCCCHHHHHHHHHCCCCEEEEEECCEEEEEHHCCCCCCCCHHHHHHHHHHH
CGTLGGVLAARGLIDPGDKVTAYLPELAGTAYDGCTLRHLLDMQVGIRFEEDYDDPNGDV
HHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCEEEECCCCCCCCCH
ARYRHSSGWDIAPPGVPAGDQRGFLTTLRPSGRPHGKLFHYVSPNTDVCGWVYERACGAP
HHEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHCCCC
YAKILSDYLWQPMGAAEDASITLDSMGAGRFAGGISATARDLARFGEMIRCRGLVQGRQV
HHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEE
VPGAWIDDIHDAGDRQAWANGDLAFVFPDGCYRSNWYTVDVKRRDLAAVGIHGQWIYVDI
CCCCCHHHHHCCCCCCCCCCCCEEEECCCCCCCCCEEEEEEECCCEEEEEECCEEEEEEE
ASDSVIVKLATQGKAMDIAQDHRWLAGFRAITAHLS
CCCCEEEEEECCCCEECHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6646204; 6389532; 2512123 [H]