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The map label for this gene is serA [H]

Identifier: 146342773

GI number: 146342773

Start: 6170735

End: 6172324

Strand: Reverse

Name: serA [H]

Synonym: BRADO5948

Alternate gene names: 146342773

Gene position: 6172324-6170735 (Counterclockwise)

Preceding gene: 146342774

Following gene: 146342772

Centisome position: 82.78

GC content: 65.16

Gene sequence:

>1590_bases
ATGACCAAGCCCAAAGTTCTCATTTCCGACGCTCTCTCCCCGGCCGCCGTGCAGATCTTTCGGGATCGCGGCGTCGAGGT
CGACTTCCAGCCCAACCTAGGCAAGGACAAGGATAAGCTCGCCGACATCATCGGCGACTATGACGGCCTCGCCATTCGCT
CCGCGACGAAGGCGACCGCGAAGATCATCGAGCGCGCCAAGCGGCTCAAGGTGATCGGCCGCGCCGGCATCGGCGTCGAC
AATGTCGAGATTCCCGCGGCGACCGCCAAGGGCATCATCGTGATGAACACGCCGTTCGGCAACTCGATCACGACCGCCGA
GCATGCGATCACCATGATGCTGTCGCTGGCGCGCGAAATCCCGCAGGCGGATGCCTCGACGCAGGCCGGCAAGTGGGAGA
AAAACCGCTTCATGGGCGTCGAGATCACCGGCAAGACGCTGGGCGTCGTCGGCTGCGGCAATATCGGCTCGATCGTCTGC
GACCGCGCGCAAGGACTGCGCATGAAGGTGATCGGCTTCGATCCGTTCCTGACCGAGGAGCGCGCCCGTGACCTCGGCGT
CGAGAAGGTCGAGCTCGACGAGTTGCTCAAGCGCGCCGACTTCATCACCTTGCACACGCCGCTGACCGACAAGACCCGCA
ACATCATCGATGCCCAGGCGATCGCCAAGATGAAACAGGGCGTGCGCATCATCAATTGCGCGCGCGGCGGCCTGATCGAC
GAGGCGGCGCTGCTCGAGGCGCTAAACTCCAAGCAGGTTGCGGGTGCCGCCCTCGACGTGTTCGCCGAGGAGCCGGCGAC
CAGCAATCCGCTGTTCGGTCATCCCGGCGTGATCTGCACGCCGCATCTCGGCGCCTCCACGACCGAAGCGCAGGAGAACG
TCGCGTTGCAGATCGCCGAGCAGATGTCGGACTATCTGCTGACGGGCGCGATCTCGAACGCCATCAACTTTCCCTCGATC
ACGGCGGAAGAGGCGCCGAAGCTGAAACCTTTCATCGCGCTGGCCGAGAAGCTCGGCTCGTTCGCGGGCCAGCTGACCGA
GAGCAGCATCACCAAGGTCCAGATCACCTATGAGGGCCAGGTCGCGGAGATGAAGACCAAGGCGCTGACCTCCGCCGTGC
TGGCGGGGCTGCTGCGGCCGATGCTGGGCGAGGTCAACGTGGTGTCCGCGCCGGTCATCGCCAAGGAGCGCGGCATGATC
GTCGACGAGGTGCTGCGCGCGGGCGAGAGCGACTACGAGAGCCTGATCTCGGTCGCGGTCACCACCGGGCAGCAGGAACG
CGCGGTGTCCGGCACGGTCTATGCCGACGGCAAGCCGCGGCTGGTCGACATCAAGGGCATCCGCGTCGACGCCGAGTTCG
GCAAATCGATGATTTACGTGACGAACGAGGACAAGCCGGGTTTCATCGGCAAGTTCGCGAGCCTGCTCGGCGACGCCAAG
ATCAACATCGCGACCTTCCACCTCGGCCGCGTCGCGCCGGGAAGCGACGCAATCGCGCTGGTCGAGGTCGACGGCGCCGT
GCCGGCCGAACTGCTTGCGAAGATCCAGGCGCTGCCCCAGGTCAAGCAGGCCAAGGCGCTGGCGTTCTGA

Upstream 100 bases:

>100_bases
CGAGAGGGCGGTGGGCACGCTTCGCTTTGCCCACCCTACAGCTTTCCCTATGCCGGCGCGTTGCGCCGTCCCTCACAAGA
GGGCGTGAAGGACCATCGTC

Downstream 100 bases:

>100_bases
TCGCAAGATCCCACAGGTCGACCAAAAAAGGCAGCACGGCACCGCGCCGCGCTGCCTTTTTTGATTTTGCGTGGCATTCG
ACGGATCGCGCTTAGCGCGG

Product: D-3-phosphoglycerate dehydrogenase

Products: NA

Alternate protein names: PGDH [H]

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MTKPKVLISDALSPAAVQIFRDRGVEVDFQPNLGKDKDKLADIIGDYDGLAIRSATKATAKIIERAKRLKVIGRAGIGVD
NVEIPAATAKGIIVMNTPFGNSITTAEHAITMMLSLAREIPQADASTQAGKWEKNRFMGVEITGKTLGVVGCGNIGSIVC
DRAQGLRMKVIGFDPFLTEERARDLGVEKVELDELLKRADFITLHTPLTDKTRNIIDAQAIAKMKQGVRIINCARGGLID
EAALLEALNSKQVAGAALDVFAEEPATSNPLFGHPGVICTPHLGASTTEAQENVALQIAEQMSDYLLTGAISNAINFPSI
TAEEAPKLKPFIALAEKLGSFAGQLTESSITKVQITYEGQVAEMKTKALTSAVLAGLLRPMLGEVNVVSAPVIAKERGMI
VDEVLRAGESDYESLISVAVTTGQQERAVSGTVYADGKPRLVDIKGIRVDAEFGKSMIYVTNEDKPGFIGKFASLLGDAK
INIATFHLGRVAPGSDAIALVEVDGAVPAELLAKIQALPQVKQAKALAF

Sequences:

>Translated_529_residues
MTKPKVLISDALSPAAVQIFRDRGVEVDFQPNLGKDKDKLADIIGDYDGLAIRSATKATAKIIERAKRLKVIGRAGIGVD
NVEIPAATAKGIIVMNTPFGNSITTAEHAITMMLSLAREIPQADASTQAGKWEKNRFMGVEITGKTLGVVGCGNIGSIVC
DRAQGLRMKVIGFDPFLTEERARDLGVEKVELDELLKRADFITLHTPLTDKTRNIIDAQAIAKMKQGVRIINCARGGLID
EAALLEALNSKQVAGAALDVFAEEPATSNPLFGHPGVICTPHLGASTTEAQENVALQIAEQMSDYLLTGAISNAINFPSI
TAEEAPKLKPFIALAEKLGSFAGQLTESSITKVQITYEGQVAEMKTKALTSAVLAGLLRPMLGEVNVVSAPVIAKERGMI
VDEVLRAGESDYESLISVAVTTGQQERAVSGTVYADGKPRLVDIKGIRVDAEFGKSMIYVTNEDKPGFIGKFASLLGDAK
INIATFHLGRVAPGSDAIALVEVDGAVPAELLAKIQALPQVKQAKALAF
>Mature_528_residues
TKPKVLISDALSPAAVQIFRDRGVEVDFQPNLGKDKDKLADIIGDYDGLAIRSATKATAKIIERAKRLKVIGRAGIGVDN
VEIPAATAKGIIVMNTPFGNSITTAEHAITMMLSLAREIPQADASTQAGKWEKNRFMGVEITGKTLGVVGCGNIGSIVCD
RAQGLRMKVIGFDPFLTEERARDLGVEKVELDELLKRADFITLHTPLTDKTRNIIDAQAIAKMKQGVRIINCARGGLIDE
AALLEALNSKQVAGAALDVFAEEPATSNPLFGHPGVICTPHLGASTTEAQENVALQIAEQMSDYLLTGAISNAINFPSIT
AEEAPKLKPFIALAEKLGSFAGQLTESSITKVQITYEGQVAEMKTKALTSAVLAGLLRPMLGEVNVVSAPVIAKERGMIV
DEVLRAGESDYESLISVAVTTGQQERAVSGTVYADGKPRLVDIKGIRVDAEFGKSMIYVTNEDKPGFIGKFASLLGDAKI
NIATFHLGRVAPGSDAIALVEVDGAVPAELLAKIQALPQVKQAKALAF

Specific function: Serine biosynthesis; first step. [C]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=501, Percent_Identity=40.7185628742515, Blast_Score=349, Evalue=5e-96,
Organism=Homo sapiens, GI4557497, Length=282, Percent_Identity=34.0425531914894, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI61743967, Length=282, Percent_Identity=34.0425531914894, Blast_Score=146, Evalue=6e-35,
Organism=Homo sapiens, GI145580578, Length=256, Percent_Identity=37.109375, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI4557499, Length=256, Percent_Identity=37.109375, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI145580575, Length=262, Percent_Identity=36.6412213740458, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI6912396, Length=244, Percent_Identity=30.7377049180328, Blast_Score=112, Evalue=7e-25,
Organism=Escherichia coli, GI1789279, Length=327, Percent_Identity=36.3914373088685, Blast_Score=188, Evalue=7e-49,
Organism=Escherichia coli, GI87082289, Length=320, Percent_Identity=30.625, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI1787645, Length=205, Percent_Identity=35.609756097561, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1788660, Length=269, Percent_Identity=31.5985130111524, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI87081824, Length=264, Percent_Identity=27.2727272727273, Blast_Score=83, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17532191, Length=308, Percent_Identity=48.0519480519481, Blast_Score=289, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI25147481, Length=251, Percent_Identity=34.6613545816733, Blast_Score=149, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6322116, Length=332, Percent_Identity=34.9397590361446, Blast_Score=213, Evalue=5e-56,
Organism=Saccharomyces cerevisiae, GI6320925, Length=331, Percent_Identity=34.441087613293, Blast_Score=209, Evalue=8e-55,
Organism=Saccharomyces cerevisiae, GI6324055, Length=272, Percent_Identity=30.5147058823529, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6324964, Length=270, Percent_Identity=25.9259259259259, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6325144, Length=115, Percent_Identity=39.1304347826087, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324980, Length=196, Percent_Identity=25, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI19921140, Length=302, Percent_Identity=45.6953642384106, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24646446, Length=258, Percent_Identity=34.8837209302326, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24646448, Length=258, Percent_Identity=34.8837209302326, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24646452, Length=258, Percent_Identity=34.8837209302326, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24646450, Length=258, Percent_Identity=34.8837209302326, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI62472511, Length=258, Percent_Identity=34.8837209302326, Blast_Score=132, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24585516, Length=289, Percent_Identity=29.0657439446367, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI28574286, Length=244, Percent_Identity=28.6885245901639, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24585514, Length=239, Percent_Identity=28.4518828451883, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI28574282, Length=239, Percent_Identity=28.4518828451883, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI28574284, Length=239, Percent_Identity=28.4518828451883, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI45551003, Length=239, Percent_Identity=28.4518828451883, Blast_Score=112, Evalue=8e-25,
Organism=Drosophila melanogaster, GI45552429, Length=239, Percent_Identity=28.4518828451883, Blast_Score=112, Evalue=9e-25,
Organism=Drosophila melanogaster, GI28571528, Length=272, Percent_Identity=27.9411764705882, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR006236
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR015508
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C; PF01842 ACT [H]

EC number: =1.1.1.95 [H]

Molecular weight: Translated: 56178; Mature: 56046

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPKVLISDALSPAAVQIFRDRGVEVDFQPNLGKDKDKLADIIGDYDGLAIRSATKATA
CCCCCEEHHCCCCHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEHHHHHHH
KIIERAKRLKVIGRAGIGVDNVEIPAATAKGIIVMNTPFGNSITTAEHAITMMLSLAREI
HHHHHHHHHHHHHCCCCCCCCEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHC
PQADASTQAGKWEKNRFMGVEITGKTLGVVGCGNIGSIVCDRAQGLRMKVIGFDPFLTEE
CCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCHHHHHHHCCCCCEEEEEECCCCCCHH
RARDLGVEKVELDELLKRADFITLHTPLTDKTRNIIDAQAIAKMKQGVRIINCARGGLID
HHHHCCCCEECHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCH
EAALLEALNSKQVAGAALDVFAEEPATSNPLFGHPGVICTPHLGASTTEAQENVALQIAE
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHH
QMSDYLLTGAISNAINFPSITAEEAPKLKPFIALAEKLGSFAGQLTESSITKVQITYEGQ
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
VAEMKTKALTSAVLAGLLRPMLGEVNVVSAPVIAKERGMIVDEVLRAGESDYESLISVAV
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEECCCCCHHHHHHHCCCHHHHHHHHHHH
TTGQQERAVSGTVYADGKPRLVDIKGIRVDAEFGKSMIYVTNEDKPGFIGKFASLLGDAK
CCCCCHHCEECEEEECCCCEEEEEEEEEEEHHHCCEEEEEECCCCCCHHHHHHHHHCCCE
INIATFHLGRVAPGSDAIALVEVDGAVPAELLAKIQALPQVKQAKALAF
EEEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHCCHHHHHHHCCC
>Mature Secondary Structure 
TKPKVLISDALSPAAVQIFRDRGVEVDFQPNLGKDKDKLADIIGDYDGLAIRSATKATA
CCCCEEHHCCCCHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEHHHHHHH
KIIERAKRLKVIGRAGIGVDNVEIPAATAKGIIVMNTPFGNSITTAEHAITMMLSLAREI
HHHHHHHHHHHHHCCCCCCCCEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHC
PQADASTQAGKWEKNRFMGVEITGKTLGVVGCGNIGSIVCDRAQGLRMKVIGFDPFLTEE
CCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCHHHHHHHCCCCCEEEEEECCCCCCHH
RARDLGVEKVELDELLKRADFITLHTPLTDKTRNIIDAQAIAKMKQGVRIINCARGGLID
HHHHCCCCEECHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCH
EAALLEALNSKQVAGAALDVFAEEPATSNPLFGHPGVICTPHLGASTTEAQENVALQIAE
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHH
QMSDYLLTGAISNAINFPSITAEEAPKLKPFIALAEKLGSFAGQLTESSITKVQITYEGQ
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
VAEMKTKALTSAVLAGLLRPMLGEVNVVSAPVIAKERGMIVDEVLRAGESDYESLISVAV
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEECCCCCHHHHHHHCCCHHHHHHHHHHH
TTGQQERAVSGTVYADGKPRLVDIKGIRVDAEFGKSMIYVTNEDKPGFIGKFASLLGDAK
CCCCCHHCEECEEEECCCCEEEEEEEEEEEHHHCCEEEEEECCCCCCHHHHHHHHHCCCE
INIATFHLGRVAPGSDAIALVEVDGAVPAELLAKIQALPQVKQAKALAF
EEEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377; 7934829 [H]