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The map label for this gene is amiC [H]

Identifier: 146342709

GI number: 146342709

Start: 6094966

End: 6096132

Strand: Reverse

Name: amiC [H]

Synonym: BRADO5876

Alternate gene names: 146342709

Gene position: 6096132-6094966 (Counterclockwise)

Preceding gene: 146342710

Following gene: 146342708

Centisome position: 81.75

GC content: 64.01

Gene sequence:

>1167_bases
ATGGGTTCTTCAGAGAGGCAGCCTGAAAGCTGGCGTGTCGGCGTACTGTTTTCGCAGACCGGCGTGACCTCGGGCATCGA
ACGGACACAACTGAACGCAACTCTGCTCGCGATCTCCGAGATCAACGCCGCCGGAGGCATCCTGGGGCGCCCGGTCGAGC
CGATCATCTACGATCCAGCGTCCGACCCCAAAAAATTCCGCGCCTTCGCCGAGCGGCTGTTCCAGGTTGACCGCATCCGT
CTGCTGTTCGGCTGCTACATGTCGAGCACGCGCAAGGCCGTGTTGCCGTCGGTCGAAGGCCATCGCGGCCTGCTGTTCTA
CCCGACCCTGTACGAAGGCTTCGAGTACTCGCGCAACTGCATCTATACCGGCGCCGCCCCCAACCAGAATTCGTTGCAGC
TCGCGCGCTTTCTGCTCTCGACCTACGGCAACCGCTTCCTGTTCGTCGGCTCGAACTATGTCTACCCCTACGAATCCAAC
CGGCTGATGGCCGATTTTGTCGTTCAGGGGAAGGGCAAGGTGCTGGACGAGATCTACGTGCCGCTGCACGCGCAGGCCAA
GGATTTCGACAAGGTCATCGCCCGCATCAAGAAGACCTCGCCGGACGTGATCTTCTCGACCGTGGTCGGCAGCGGAACGG
CCGATTTCTACCGCGCCTATCGCGCCGCCGGCTTCGATCCGACGCGGATGCCGATCGCGAGCCTGACCACCAGCGAGGCC
GAGGTCGCCGAGATGGGCGCCGAATCCGCCGAGGGCCACATCACCGCCGCGCCGTTCTTCGAGACGCTGTCGTCACAGGC
CGCGCGCCGCTTCGTCGCCAGCTTCAAGTCGAAGTATGGCGACGATGCGCCGGCGACGGCGTGCGCGGAGGCCGCCTACT
TCCAGGTGCATCTGGCGATGCGGGCGGTGGCCAAATGCGGCTCCGATGATCCGGAGCAGGTGCTCGGCAACATGCGCGAT
TTCGAATTCGACGCGCCGCAGGGGCGGGTTCGGGTCGATCCCGAGAACAACCACACCTATCTGTGGCCGCGCATCGCGCG
CGTCGACGGCCAGGGACGGTTCCAGATCGTCTGGAATCCCGGCGTTCGGGTGAAGCCGGATCCGTATTGCGTGGTCCAGA
GTCTCGACGACTGGTCGGTCGACGAGCAGCAACTCATTCGGTCCTGA

Upstream 100 bases:

>100_bases
CGAGCAAACAATCATCCTAGACAGAACCAGGTGGCGTTCCCTCGGCACGGCCGGTCGATGGTGTCGGGAGACGCATTCAG
GAGCGTGGTGCTGTCGTCCT

Downstream 100 bases:

>100_bases
GGTCAGGGGTAGCGTGGCGGGGCGGAGAACCGGGCGTGGCGATCCAAATTCTTCGTGATCTGCGCGGCCTGCGGGTCCAT
GTCGTACACCCGCCGGACAG

Product: putative aliphatic amidase expression-regulating protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MGSSERQPESWRVGVLFSQTGVTSGIERTQLNATLLAISEINAAGGILGRPVEPIIYDPASDPKKFRAFAERLFQVDRIR
LLFGCYMSSTRKAVLPSVEGHRGLLFYPTLYEGFEYSRNCIYTGAAPNQNSLQLARFLLSTYGNRFLFVGSNYVYPYESN
RLMADFVVQGKGKVLDEIYVPLHAQAKDFDKVIARIKKTSPDVIFSTVVGSGTADFYRAYRAAGFDPTRMPIASLTTSEA
EVAEMGAESAEGHITAAPFFETLSSQAARRFVASFKSKYGDDAPATACAEAAYFQVHLAMRAVAKCGSDDPEQVLGNMRD
FEFDAPQGRVRVDPENNHTYLWPRIARVDGQGRFQIVWNPGVRVKPDPYCVVQSLDDWSVDEQQLIRS

Sequences:

>Translated_388_residues
MGSSERQPESWRVGVLFSQTGVTSGIERTQLNATLLAISEINAAGGILGRPVEPIIYDPASDPKKFRAFAERLFQVDRIR
LLFGCYMSSTRKAVLPSVEGHRGLLFYPTLYEGFEYSRNCIYTGAAPNQNSLQLARFLLSTYGNRFLFVGSNYVYPYESN
RLMADFVVQGKGKVLDEIYVPLHAQAKDFDKVIARIKKTSPDVIFSTVVGSGTADFYRAYRAAGFDPTRMPIASLTTSEA
EVAEMGAESAEGHITAAPFFETLSSQAARRFVASFKSKYGDDAPATACAEAAYFQVHLAMRAVAKCGSDDPEQVLGNMRD
FEFDAPQGRVRVDPENNHTYLWPRIARVDGQGRFQIVWNPGVRVKPDPYCVVQSLDDWSVDEQQLIRS
>Mature_387_residues
GSSERQPESWRVGVLFSQTGVTSGIERTQLNATLLAISEINAAGGILGRPVEPIIYDPASDPKKFRAFAERLFQVDRIRL
LFGCYMSSTRKAVLPSVEGHRGLLFYPTLYEGFEYSRNCIYTGAAPNQNSLQLARFLLSTYGNRFLFVGSNYVYPYESNR
LMADFVVQGKGKVLDEIYVPLHAQAKDFDKVIARIKKTSPDVIFSTVVGSGTADFYRAYRAAGFDPTRMPIASLTTSEAE
VAEMGAESAEGHITAAPFFETLSSQAARRFVASFKSKYGDDAPATACAEAAYFQVHLAMRAVAKCGSDDPEQVLGNMRDF
EFDAPQGRVRVDPENNHTYLWPRIARVDGQGRFQIVWNPGVRVKPDPYCVVQSLDDWSVDEQQLIRS

Specific function: Negatively regulates the expression of the aliphatic amidase operon. AmiC functions by inhibiting the action of AmiR at the protein level. It exhibits protein kinase activity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI48994941, Length=334, Percent_Identity=22.7544910179641, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000709 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 43132; Mature: 43001

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSSERQPESWRVGVLFSQTGVTSGIERTQLNATLLAISEINAAGGILGRPVEPIIYDPA
CCCCCCCCCCEEEEEEEECCCCCCCCHHHCCCEEEEEEECCCCCCCCCCCCCCCEEECCC
SDPKKFRAFAERLFQVDRIRLLFGCYMSSTRKAVLPSVEGHRGLLFYPTLYEGFEYSRNC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCEEECCHHHCCCCCCCCE
IYTGAAPNQNSLQLARFLLSTYGNRFLFVGSNYVYPYESNRLMADFVVQGKGKVLDEIYV
EEECCCCCCCHHHHHHHHHHHCCCEEEEECCCEEEECCCCCEEEEEEECCCCCEEHHHCC
PLHAQAKDFDKVIARIKKTSPDVIFSTVVGSGTADFYRAYRAAGFDPTRMPIASLTTSEA
CCCCCHHHHHHHHHHHHCCCCCEEEEEHHCCCHHHHHHHHHHCCCCCCCCCHHHCCCCHH
EVAEMGAESAEGHITAAPFFETLSSQAARRFVASFKSKYGDDAPATACAEAAYFQVHLAM
HHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
RAVAKCGSDDPEQVLGNMRDFEFDAPQGRVRVDPENNHTYLWPRIARVDGQGRFQIVWNP
HHHHHCCCCCHHHHHCCCCCCCCCCCCCCEEECCCCCCEEEEEEEEEECCCCEEEEEECC
GVRVKPDPYCVVQSLDDWSVDEQQLIRS
CCEECCCCEEEEECCCCCCCCHHHHHCC
>Mature Secondary Structure 
GSSERQPESWRVGVLFSQTGVTSGIERTQLNATLLAISEINAAGGILGRPVEPIIYDPA
CCCCCCCCCEEEEEEEECCCCCCCCHHHCCCEEEEEEECCCCCCCCCCCCCCCEEECCC
SDPKKFRAFAERLFQVDRIRLLFGCYMSSTRKAVLPSVEGHRGLLFYPTLYEGFEYSRNC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCEEECCHHHCCCCCCCCE
IYTGAAPNQNSLQLARFLLSTYGNRFLFVGSNYVYPYESNRLMADFVVQGKGKVLDEIYV
EEECCCCCCCHHHHHHHHHHHCCCEEEEECCCEEEECCCCCEEEEEEECCCCCEEHHHCC
PLHAQAKDFDKVIARIKKTSPDVIFSTVVGSGTADFYRAYRAAGFDPTRMPIASLTTSEA
CCCCCHHHHHHHHHHHHCCCCCEEEEEHHCCCHHHHHHHHHHCCCCCCCCCHHHCCCCHH
EVAEMGAESAEGHITAAPFFETLSSQAARRFVASFKSKYGDDAPATACAEAAYFQVHLAM
HHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
RAVAKCGSDDPEQVLGNMRDFEFDAPQGRVRVDPENNHTYLWPRIARVDGQGRFQIVWNP
HHHHHCCCCCHHHHHCCCCCCCCCCCCCCEEECCCCCCEEEEEEEEEECCCCEEEEEECC
GVRVKPDPYCVVQSLDDWSVDEQQLIRS
CCEECCCCEEEEECCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1907262; 10984043; 1762155; 7813419; 10508151; 10708652 [H]