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The map label for this gene is emrA [H]

Identifier: 146341985

GI number: 146341985

Start: 5320576

End: 5321847

Strand: Reverse

Name: emrA [H]

Synonym: BRADO5121

Alternate gene names: 146341985

Gene position: 5321847-5320576 (Counterclockwise)

Preceding gene: 146341986

Following gene: 146341984

Centisome position: 71.37

GC content: 69.34

Gene sequence:

>1272_bases
ATGGCCGTGCAGTCGTCGTCCACCACCGAGTCCACGCCCCCCGCCAGACCCGCCGAGCAGACCGTGCCGGCGCGCGAGCG
CGCGCTGAAGCAATGGCGTGGCGACGCCGTGAAGGAGCTCCGCCGCGAGGCCGACCGGGAAACGCAAGAGCCGGATCAGA
AAGCTCCCGCTCCCGAGGAACGCAGCGAGCAGGGCGAGAAGCCGCGCAGGGGACTGCTGCGGCGGCACCCCGTGGGCGCA
GCGGTCGGTCTCGTCGTGCTGCTGGCGGCCGCCGCCGGCGGCTATGTCTATTTCGACGCCGCCAGCCATTTCGAGAGCAC
CGATGACGCCTTCATCGCAGCCCGCCAGTTCTCGATCGCGCCGAAGGTCTCCGGCTACATCACCTCCGTCCCGGTCACCG
ACAACCAGCACGTCAAGGCCGGCGACGTGATCGCGCGCATCGACGATCGCGACTACAAGACGGCGCTGGCGCAGGCCGAG
GCGCAGGTGCGGTCGGCGGAGGCCGCGATCCACAACATCGACGCGCAGATTGCGGTTCAGCAGGCACAGATCACCGCGGG
CCAGGCGCAGGTCGACCAGTCCCAGGCCGGGCTCACCTTCGCCCAGCAGCAGGCCGACCGCTACCAGCAGCTCGCGCGCA
CCGGATCCGGCACCGTGCAGAGCGAGCAGCAATACACCTCTCAGCTCCAGCAGGCGAAGGCCGCGTTGACCTCGTCGCAG
GCCAATCTCCAGGTCGCGCAGCGCCAGCTGGAATCGCTCAAAGCCCAGCGCGAGAGTGCGGACGCCAATCTCGCCCAGGC
GCAGGCGCAGCGCGACCAGGCGCAGCTCAATCTGTCCTACACGGTCGTGACCGCGGCGCAGCCCGGCCGCGTCGTCAATC
TCAGCGCCGCCGACGGCCAGTTCGCCGCGCAAGGCACCGCGCTGTCGATGTTCGTGCCCGACGAGGTCTGGGTCACCGCC
AATTTCAAGGAGACCCAGCTCGACCAGATGCGTCCCGGCCAGCCGGTGACCCTGGAGATCGACGCCTATCCGGAGCGCGA
GATCCGCGGCCATGTCGCCAGCGTGCAGTCCGGCTCGGGTCCGGCGTTCTCGCTGCTGCCGCCGCAGAACGCGACCGGCA
ACTACGTCAAGATCGTGCAGCGCGTGCCCGTGAAGATCGACATGGACGATCCGCCGACGGATGTCGCGCTCGGCCCGGGC
ATGTCGGTGGTGCCGACGGTGCGCGTCGATCCCAAGCCGTCGCTCTATGAGCGGCTGAAAGCCATGCTGTGA

Upstream 100 bases:

>100_bases
CACGAAAAGTTATCGATCCCGAAGCGGGGGTTCGGGCTTGCCTGTTCAGATCCCTCGCCTATTTTAGAACGAAACAGTAT
CGTTCTTTTCGGAGCGCCGC

Downstream 100 bases:

>100_bases
TGAGGCTGATGCCATGAGCGAGTCTGCTGCTCTCTCCGCGCCGCGCGCCGGCAGTCCGTGGATCATCGCGGTGCTGGTGG
CGCTGGCGACCTTCATGGAG

Product: HlyD family multidrug resistance protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MAVQSSSTTESTPPARPAEQTVPARERALKQWRGDAVKELRREADRETQEPDQKAPAPEERSEQGEKPRRGLLRRHPVGA
AVGLVVLLAAAAGGYVYFDAASHFESTDDAFIAARQFSIAPKVSGYITSVPVTDNQHVKAGDVIARIDDRDYKTALAQAE
AQVRSAEAAIHNIDAQIAVQQAQITAGQAQVDQSQAGLTFAQQQADRYQQLARTGSGTVQSEQQYTSQLQQAKAALTSSQ
ANLQVAQRQLESLKAQRESADANLAQAQAQRDQAQLNLSYTVVTAAQPGRVVNLSAADGQFAAQGTALSMFVPDEVWVTA
NFKETQLDQMRPGQPVTLEIDAYPEREIRGHVASVQSGSGPAFSLLPPQNATGNYVKIVQRVPVKIDMDDPPTDVALGPG
MSVVPTVRVDPKPSLYERLKAML

Sequences:

>Translated_423_residues
MAVQSSSTTESTPPARPAEQTVPARERALKQWRGDAVKELRREADRETQEPDQKAPAPEERSEQGEKPRRGLLRRHPVGA
AVGLVVLLAAAAGGYVYFDAASHFESTDDAFIAARQFSIAPKVSGYITSVPVTDNQHVKAGDVIARIDDRDYKTALAQAE
AQVRSAEAAIHNIDAQIAVQQAQITAGQAQVDQSQAGLTFAQQQADRYQQLARTGSGTVQSEQQYTSQLQQAKAALTSSQ
ANLQVAQRQLESLKAQRESADANLAQAQAQRDQAQLNLSYTVVTAAQPGRVVNLSAADGQFAAQGTALSMFVPDEVWVTA
NFKETQLDQMRPGQPVTLEIDAYPEREIRGHVASVQSGSGPAFSLLPPQNATGNYVKIVQRVPVKIDMDDPPTDVALGPG
MSVVPTVRVDPKPSLYERLKAML
>Mature_422_residues
AVQSSSTTESTPPARPAEQTVPARERALKQWRGDAVKELRREADRETQEPDQKAPAPEERSEQGEKPRRGLLRRHPVGAA
VGLVVLLAAAAGGYVYFDAASHFESTDDAFIAARQFSIAPKVSGYITSVPVTDNQHVKAGDVIARIDDRDYKTALAQAEA
QVRSAEAAIHNIDAQIAVQQAQITAGQAQVDQSQAGLTFAQQQADRYQQLARTGSGTVQSEQQYTSQLQQAKAALTSSQA
NLQVAQRQLESLKAQRESADANLAQAQAQRDQAQLNLSYTVVTAAQPGRVVNLSAADGQFAAQGTALSMFVPDEVWVTAN
FKETQLDQMRPGQPVTLEIDAYPEREIRGHVASVQSGSGPAFSLLPPQNATGNYVKIVQRVPVKIDMDDPPTDVALGPGM
SVVPTVRVDPKPSLYERLKAML

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=377, Percent_Identity=33.1564986737401, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI1788711, Length=333, Percent_Identity=32.1321321321321, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1790519, Length=295, Percent_Identity=31.864406779661, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI87081951, Length=329, Percent_Identity=29.1793313069909, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789637, Length=355, Percent_Identity=28.7323943661972, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789900, Length=292, Percent_Identity=27.7397260273973, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1787013, Length=253, Percent_Identity=28.8537549407115, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 45641; Mature: 45510

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVQSSSTTESTPPARPAEQTVPARERALKQWRGDAVKELRREADRETQEPDQKAPAPEE
CCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCHH
RSEQGEKPRRGLLRRHPVGAAVGLVVLLAAAAGGYVYFDAASHFESTDDAFIAARQFSIA
HHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECHHHCCCCCCCEEEEHHEECC
PKVSGYITSVPVTDNQHVKAGDVIARIDDRDYKTALAQAEAQVRSAEAAIHNIDAQIAVQ
CCCCCEEEECCCCCCCCEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHEEEE
QAQITAGQAQVDQSQAGLTFAQQQADRYQQLARTGSGTVQSEQQYTSQLQQAKAALTSSQ
HHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCH
ANLQVAQRQLESLKAQRESADANLAQAQAQRDQAQLNLSYTVVTAAQPGRVVNLSAADGQ
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEEEEEECCCCEEEEEECCCCC
FAAQGTALSMFVPDEVWVTANFKETQLDQMRPGQPVTLEIDAYPEREIRGHVASVQSGSG
EEECCCEEEEEECCCEEEEECCCHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCC
PAFSLLPPQNATGNYVKIVQRVPVKIDMDDPPTDVALGPGMSVVPTVRVDPKPSLYERLK
CEEEECCCCCCCCHHHHHHHHCCEEEECCCCCCCEEECCCCCEEEEEEECCCHHHHHHHH
AML
HCC
>Mature Secondary Structure 
AVQSSSTTESTPPARPAEQTVPARERALKQWRGDAVKELRREADRETQEPDQKAPAPEE
CCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCHH
RSEQGEKPRRGLLRRHPVGAAVGLVVLLAAAAGGYVYFDAASHFESTDDAFIAARQFSIA
HHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECHHHCCCCCCCEEEEHHEECC
PKVSGYITSVPVTDNQHVKAGDVIARIDDRDYKTALAQAEAQVRSAEAAIHNIDAQIAVQ
CCCCCEEEECCCCCCCCEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHEEEE
QAQITAGQAQVDQSQAGLTFAQQQADRYQQLARTGSGTVQSEQQYTSQLQQAKAALTSSQ
HHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCH
ANLQVAQRQLESLKAQRESADANLAQAQAQRDQAQLNLSYTVVTAAQPGRVVNLSAADGQ
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEEEEEECCCCEEEEEECCCCC
FAAQGTALSMFVPDEVWVTANFKETQLDQMRPGQPVTLEIDAYPEREIRGHVASVQSGSG
EEECCCEEEEEECCCEEEEECCCHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCC
PAFSLLPPQNATGNYVKIVQRVPVKIDMDDPPTDVALGPGMSVVPTVRVDPKPSLYERLK
CEEEECCCCCCCCHHHHHHHHCCEEEECCCCCCCEEECCCCCEEEEEEECCCHHHHHHHH
AML
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]