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The map label for this gene is emrB [H]

Identifier: 146341984

GI number: 146341984

Start: 5318982

End: 5320562

Strand: Reverse

Name: emrB [H]

Synonym: BRADO5120

Alternate gene names: 146341984

Gene position: 5320562-5318982 (Counterclockwise)

Preceding gene: 146341985

Following gene: 146341983

Centisome position: 71.35

GC content: 65.59

Gene sequence:

>1581_bases
ATGAGCGAGTCTGCTGCTCTCTCCGCGCCGCGCGCCGGCAGTCCGTGGATCATCGCGGTGCTGGTGGCGCTGGCGACCTT
CATGGAGGTGCTCGACACCACCATCGCCAATGTCGCGCTGCCCTATATCGCGGGCGGCATGGGCGTGAGCCAGGACGAAT
CCTCCTGGGTGGTGACGACCTATCTGGTCTCCAACGCGATCATCCTGACGGCGTCGAGCTATCTGGCCAAGCGGTTCGGC
CGCAAGGCTTTCTTCATCATGTGCCTCGGCCTGTTCACCACGAGCTCGGTGCTGTGCGGCTTTGCCTGGAATCTGCAGGT
GCTGTTGATGTTCCGCATCCTACAGGGGCTCGGCGGCGGCGGCATGGTGCCGGTCGCGCAATCGATCCTCGCCGACTCCT
TCCCACCGGAAAAGCGCGGCCAGGCCTTCGCGCTGTTCGGTGTCGCCGTCGTCGTCGCGCCGGTCGTGGGCCCGACGCTC
GGCGGCTATCTCTCGGACAACGCCTCGTGGCACTGGTGCTTCCTGATCAACGGTCCCGTCGGAGTCGCCGCGATGCTGCT
GATCTGGCTGATCCTCAAGGAGCCGGCAGAGGTGACGGAAGAGCGGCGGAAGCAGGCGCATGGCTTCGACGTGGTCGGAT
TCGTGCTGGTCGCGACCTTCCTCGGCGCGCTCGAGCTGATGCTCGACCGCGGCCTGGAGGACGACTGGTTCGACTCCCGC
TTCATCGTGATCACCGCCGCGGTCTGCGCCACGGCCTTCGTCGCCATGATCCCGTGGGAGATGAACCAGAAGAACCCGAC
GGTCGACGTGCGCATGCTCGCCTCGCGGCAGTTCGGCGCCTGCTTCCTGGTGATGCTGGCGACCGGCGCGATCCTGCTCG
GCACCACGCAGATGCTGCCGCAGATGGTGCAGCAGGATTTCGGCTACACCGCCACCTGGGCCGGTCTCGTGCTGTCGCCG
GGCGGCCTGGTCACCATGGCCATGATGTTCGTGGTCGGCCGGCTCTCCGGCAAGGTACAGCCGAAATACATGATCATGAC
CGGCGCCGCGGTGGTGGCGTTCGCCATGTATGACCTGACGCGTGTCTACGCCAATGTCGACTTCTGGTTCTTCGCCGAGT
CCCGCATGCTGATTGGCATCGGCCTGCCGCTGATCTTCCTGTCGATCACGACGGCGTCCTACAACGGCATTCCCCCTGAA
CGAACCGACCAGGCCTCGGCCCTGATCAACGCGGCCCGCAACACCGGCGGCTCCATCGGCGTGTCCTTGGCGGCCAACAT
CCTGGCGCATCGCGGCCAGTTCCACCAGAACCGGCTCGTCGAGAGCACCATCCCGTCGAACCCGGCCTATCAGGACACGC
TGAACCAGGTTACCAACTACTTCGCCGCCCATGGCAGCTCGATGGTGCAGGCGAAGCAGCAGGCGCTCGCCTGGATCGGC
CAGACCGTGCAGATGCAGTCGTCGCTGCTGGCCTATGTCGACGTGTATTGGGCGCTGATGCTGATCTCGCTGTCAGCCAT
CCCGCTGGCGCTGACCCTGCGCAAGGTCAAGCTCGGCGGCGGCCCGGCGATGGCGCATTAG

Upstream 100 bases:

>100_bases
GCGCTCGGCCCGGGCATGTCGGTGGTGCCGACGGTGCGCGTCGATCCCAAGCCGTCGCTCTATGAGCGGCTGAAAGCCAT
GCTGTGATGAGGCTGATGCC

Downstream 100 bases:

>100_bases
GAAAAACTGCGTAGGGTGGGCAAAGGCGTTGCCGTACTCTTTCCTCAGCTTCCGATCGCAAAGGCGCCGTGCCCACCATC
TGGCCGCTGTGCTCGCTGAT

Product: putative multidrug resistance protein, emrB-like

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSESAALSAPRAGSPWIIAVLVALATFMEVLDTTIANVALPYIAGGMGVSQDESSWVVTTYLVSNAIILTASSYLAKRFG
RKAFFIMCLGLFTTSSVLCGFAWNLQVLLMFRILQGLGGGGMVPVAQSILADSFPPEKRGQAFALFGVAVVVAPVVGPTL
GGYLSDNASWHWCFLINGPVGVAAMLLIWLILKEPAEVTEERRKQAHGFDVVGFVLVATFLGALELMLDRGLEDDWFDSR
FIVITAAVCATAFVAMIPWEMNQKNPTVDVRMLASRQFGACFLVMLATGAILLGTTQMLPQMVQQDFGYTATWAGLVLSP
GGLVTMAMMFVVGRLSGKVQPKYMIMTGAAVVAFAMYDLTRVYANVDFWFFAESRMLIGIGLPLIFLSITTASYNGIPPE
RTDQASALINAARNTGGSIGVSLAANILAHRGQFHQNRLVESTIPSNPAYQDTLNQVTNYFAAHGSSMVQAKQQALAWIG
QTVQMQSSLLAYVDVYWALMLISLSAIPLALTLRKVKLGGGPAMAH

Sequences:

>Translated_526_residues
MSESAALSAPRAGSPWIIAVLVALATFMEVLDTTIANVALPYIAGGMGVSQDESSWVVTTYLVSNAIILTASSYLAKRFG
RKAFFIMCLGLFTTSSVLCGFAWNLQVLLMFRILQGLGGGGMVPVAQSILADSFPPEKRGQAFALFGVAVVVAPVVGPTL
GGYLSDNASWHWCFLINGPVGVAAMLLIWLILKEPAEVTEERRKQAHGFDVVGFVLVATFLGALELMLDRGLEDDWFDSR
FIVITAAVCATAFVAMIPWEMNQKNPTVDVRMLASRQFGACFLVMLATGAILLGTTQMLPQMVQQDFGYTATWAGLVLSP
GGLVTMAMMFVVGRLSGKVQPKYMIMTGAAVVAFAMYDLTRVYANVDFWFFAESRMLIGIGLPLIFLSITTASYNGIPPE
RTDQASALINAARNTGGSIGVSLAANILAHRGQFHQNRLVESTIPSNPAYQDTLNQVTNYFAAHGSSMVQAKQQALAWIG
QTVQMQSSLLAYVDVYWALMLISLSAIPLALTLRKVKLGGGPAMAH
>Mature_525_residues
SESAALSAPRAGSPWIIAVLVALATFMEVLDTTIANVALPYIAGGMGVSQDESSWVVTTYLVSNAIILTASSYLAKRFGR
KAFFIMCLGLFTTSSVLCGFAWNLQVLLMFRILQGLGGGGMVPVAQSILADSFPPEKRGQAFALFGVAVVVAPVVGPTLG
GYLSDNASWHWCFLINGPVGVAAMLLIWLILKEPAEVTEERRKQAHGFDVVGFVLVATFLGALELMLDRGLEDDWFDSRF
IVITAAVCATAFVAMIPWEMNQKNPTVDVRMLASRQFGACFLVMLATGAILLGTTQMLPQMVQQDFGYTATWAGLVLSPG
GLVTMAMMFVVGRLSGKVQPKYMIMTGAAVVAFAMYDLTRVYANVDFWFFAESRMLIGIGLPLIFLSITTASYNGIPPER
TDQASALINAARNTGGSIGVSLAANILAHRGQFHQNRLVESTIPSNPAYQDTLNQVTNYFAAHGSSMVQAKQQALAWIGQ
TVQMQSSLLAYVDVYWALMLISLSAIPLALTLRKVKLGGGPAMAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=488, Percent_Identity=37.2950819672131, Blast_Score=320, Evalue=1e-88,
Organism=Escherichia coli, GI1788710, Length=465, Percent_Identity=32.6881720430108, Blast_Score=288, Evalue=9e-79,
Organism=Escherichia coli, GI1790195, Length=479, Percent_Identity=25.678496868476, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1788392, Length=421, Percent_Identity=28.978622327791, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI87081983, Length=412, Percent_Identity=27.6699029126214, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1790146, Length=173, Percent_Identity=30.635838150289, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789312, Length=160, Percent_Identity=28.75, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=201, Percent_Identity=25.8706467661692, Blast_Score=80, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6325455, Length=133, Percent_Identity=29.3233082706767, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=28, Blast_Score=70, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6319770, Length=173, Percent_Identity=27.1676300578035, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6321577, Length=131, Percent_Identity=32.0610687022901, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24654039, Length=314, Percent_Identity=23.8853503184713, Blast_Score=66, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24654044, Length=230, Percent_Identity=24.7826086956522, Blast_Score=66, Evalue=7e-11,
Organism=Drosophila melanogaster, GI17864456, Length=314, Percent_Identity=23.8853503184713, Blast_Score=65, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24654037, Length=230, Percent_Identity=24.7826086956522, Blast_Score=65, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56573; Mature: 56442

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESAALSAPRAGSPWIIAVLVALATFMEVLDTTIANVALPYIAGGMGVSQDESSWVVTT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEH
YLVSNAIILTASSYLAKRFGRKAFFIMCLGLFTTSSVLCGFAWNLQVLLMFRILQGLGGG
HHHHCCCEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GMVPVAQSILADSFPPEKRGQAFALFGVAVVVAPVVGPTLGGYLSDNASWHWCFLINGPV
CHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCH
GVAAMLLIWLILKEPAEVTEERRKQAHGFDVVGFVLVATFLGALELMLDRGLEDDWFDSR
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
FIVITAAVCATAFVAMIPWEMNQKNPTVDVRMLASRQFGACFLVMLATGAILLGTTQMLP
HHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCHHHHHHHHHHH
QMVQQDFGYTATWAGLVLSPGGLVTMAMMFVVGRLSGKVQPKYMIMTGAAVVAFAMYDLT
HHHHHHCCCCEEEHHHEECCCHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHH
RVYANVDFWFFAESRMLIGIGLPLIFLSITTASYNGIPPERTDQASALINAARNTGGSIG
HHHHCCCEEEEECCCEEEEECHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCHH
VSLAANILAHRGQFHQNRLVESTIPSNPAYQDTLNQVTNYFAAHGSSMVQAKQQALAWIG
HHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
QTVQMQSSLLAYVDVYWALMLISLSAIPLALTLRKVKLGGGPAMAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCC
>Mature Secondary Structure 
SESAALSAPRAGSPWIIAVLVALATFMEVLDTTIANVALPYIAGGMGVSQDESSWVVTT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEH
YLVSNAIILTASSYLAKRFGRKAFFIMCLGLFTTSSVLCGFAWNLQVLLMFRILQGLGGG
HHHHCCCEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GMVPVAQSILADSFPPEKRGQAFALFGVAVVVAPVVGPTLGGYLSDNASWHWCFLINGPV
CHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCH
GVAAMLLIWLILKEPAEVTEERRKQAHGFDVVGFVLVATFLGALELMLDRGLEDDWFDSR
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
FIVITAAVCATAFVAMIPWEMNQKNPTVDVRMLASRQFGACFLVMLATGAILLGTTQMLP
HHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCHHHHHHHHHHH
QMVQQDFGYTATWAGLVLSPGGLVTMAMMFVVGRLSGKVQPKYMIMTGAAVVAFAMYDLT
HHHHHHCCCCEEEHHHEECCCHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHH
RVYANVDFWFFAESRMLIGIGLPLIFLSITTASYNGIPPERTDQASALINAARNTGGSIG
HHHHCCCEEEEECCCEEEEECHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCHH
VSLAANILAHRGQFHQNRLVESTIPSNPAYQDTLNQVTNYFAAHGSSMVQAKQQALAWIG
HHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
QTVQMQSSLLAYVDVYWALMLISLSAIPLALTLRKVKLGGGPAMAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]