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The map label for this gene is dfrA [H]

Identifier: 146341908

GI number: 146341908

Start: 5232515

End: 5233543

Strand: Reverse

Name: dfrA [H]

Synonym: BRADO5042

Alternate gene names: 146341908

Gene position: 5233543-5232515 (Counterclockwise)

Preceding gene: 146341909

Following gene: 146341907

Centisome position: 70.19

GC content: 71.23

Gene sequence:

>1029_bases
ATGAGCGAGGTTCTGGTCACCGGTGGCTCCGGCTTCATCGCGACGCATGTCATCTTGCAGCTGCTCGCGGCCGGCCATCG
CGTTCGCACCACGCTGCGCACCCCCGACCGGCAGAGCGAGGTGCTGGCCGCGCTCGCCCGCGGCGGCGCGACCGATCCCC
ATTTGCTGTCGTTCCACGCTGCCGATCTGACCCGGGATGACGGCTGGGCCGCGGCGGTGTCGGGCTGCGACTACGTCCTC
CACATCGCGTCGCCGCTGCCCGACCACGTGCCTGACGACGAGAACGAGCTGATCGTGCCGGCACGCGACGGCACGTTGCG
GGTGCTGCGGGCGGCGCGCGACGCCGGCGTGACGCGCGTGGTGCTGACTTCCTCCTTCTCCGCCGTCGCCTATGGCCATC
GCCCCCGCGCAAAACCGTTCGACGAGGCCGATTGGACCGATCTCGACGGTCCCGACGTGCAGCCTTACCCGAAGTCCAAG
ACCCTGGCCGAACGCGCCGCCTGGGCGTTTCTCGCCAGCGAGGGCGGCGCGTTGGAGCTTGCGACCATCTGTCCGGTCGC
CGTGCTCGGCCCGGTGCTGGGCCCGGATTTTTCGCCCTCCATTGCGCTGGTGCAGGCGATGCTGCAGGGCAGGGCGCCGG
CCGCGCCCAGGATCCATCTCGGGCTGGTCGACGTCCGCGACGTCGCCGACCTGCATCTGCGTGCGATGACCGACCCCGCC
GCCCGCAACGAGCGGTTCCTGGCGGTGGCCGGTTCGCCGCTGTCGATCCTCGAGATCGCGACCGTGCTGCGGACACGGCT
GGGCAACGCGGGACGACGGGCGCCGCGGCGCGAGCTGCCGAACTGGCTGGCCCGTATCGTGGCGCTCGCCGTACCGCAAT
TGCGCGACGTCAAGCCGCTGCTCGGCCTGCGCCGCACAGCCTCGAGCGACAAGGCGCGTCGGCTGCTCGGCTGGAATCCG
CGGCCGAATGATGAGATCATCGCAGCGACGGCCGAGAGCCTGCTGAGGCTGGGCCTGGTGCGGCCGTGA

Upstream 100 bases:

>100_bases
TCACCGAGCTCGTTCAGAGCTGGCGAAGCGGATCGCTCCTGACACGGCCCCGACACGGCGCTCTGCCATGCTTCGACCCT
CGGGGGGCGAGGTGAATGCA

Downstream 100 bases:

>100_bases
GGCGGTTGAACCGCCGTCTGAAGGACAGGGCTGACTTCGAGCGCGGTGCCAACTGCCGCGACAGGAGATGAAGTGAGCGG
CAAAAGCCGCGTCGTGACAA

Product: putative dihydrokaempferol 4-reductase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MSEVLVTGGSGFIATHVILQLLAAGHRVRTTLRTPDRQSEVLAALARGGATDPHLLSFHAADLTRDDGWAAAVSGCDYVL
HIASPLPDHVPDDENELIVPARDGTLRVLRAARDAGVTRVVLTSSFSAVAYGHRPRAKPFDEADWTDLDGPDVQPYPKSK
TLAERAAWAFLASEGGALELATICPVAVLGPVLGPDFSPSIALVQAMLQGRAPAAPRIHLGLVDVRDVADLHLRAMTDPA
ARNERFLAVAGSPLSILEIATVLRTRLGNAGRRAPRRELPNWLARIVALAVPQLRDVKPLLGLRRTASSDKARRLLGWNP
RPNDEIIAATAESLLRLGLVRP

Sequences:

>Translated_342_residues
MSEVLVTGGSGFIATHVILQLLAAGHRVRTTLRTPDRQSEVLAALARGGATDPHLLSFHAADLTRDDGWAAAVSGCDYVL
HIASPLPDHVPDDENELIVPARDGTLRVLRAARDAGVTRVVLTSSFSAVAYGHRPRAKPFDEADWTDLDGPDVQPYPKSK
TLAERAAWAFLASEGGALELATICPVAVLGPVLGPDFSPSIALVQAMLQGRAPAAPRIHLGLVDVRDVADLHLRAMTDPA
ARNERFLAVAGSPLSILEIATVLRTRLGNAGRRAPRRELPNWLARIVALAVPQLRDVKPLLGLRRTASSDKARRLLGWNP
RPNDEIIAATAESLLRLGLVRP
>Mature_341_residues
SEVLVTGGSGFIATHVILQLLAAGHRVRTTLRTPDRQSEVLAALARGGATDPHLLSFHAADLTRDDGWAAAVSGCDYVLH
IASPLPDHVPDDENELIVPARDGTLRVLRAARDAGVTRVVLTSSFSAVAYGHRPRAKPFDEADWTDLDGPDVQPYPKSKT
LAERAAWAFLASEGGALELATICPVAVLGPVLGPDFSPSIALVQAMLQGRAPAAPRIHLGLVDVRDVADLHLRAMTDPAA
RNERFLAVAGSPLSILEIATVLRTRLGNAGRRAPRRELPNWLARIVALAVPQLRDVKPLLGLRRTASSDKARRLLGWNPR
PNDEIIAATAESLLRLGLVRP

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=365, Percent_Identity=25.7534246575342, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI8393516, Length=365, Percent_Identity=25.7534246575342, Blast_Score=66, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71987463, Length=345, Percent_Identity=38.5507246376812, Blast_Score=213, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6320750, Length=364, Percent_Identity=26.9230769230769, Blast_Score=109, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6324421, Length=356, Percent_Identity=27.5280898876405, Blast_Score=105, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6321399, Length=262, Percent_Identity=29.0076335877863, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321281, Length=354, Percent_Identity=25.1412429378531, Blast_Score=97, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 36664; Mature: 36532

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVLVTGGSGFIATHVILQLLAAGHRVRTTLRTPDRQSEVLAALARGGATDPHLLSFHA
CCCEEEECCCCHHHHHHHHHHHHCCHHHEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEH
ADLTRDDGWAAAVSGCDYVLHIASPLPDHVPDDENELIVPARDGTLRVLRAARDAGVTRV
HHCCCCCCCHHHHCCCCEEEEECCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCEEE
VLTSSFSAVAYGHRPRAKPFDEADWTDLDGPDVQPYPKSKTLAERAAWAFLASEGGALEL
EEECCCHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEECCCCCEEH
ATICPVAVLGPVLGPDFSPSIALVQAMLQGRAPAAPRIHLGLVDVRDVADLHLRAMTDPA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHCCCC
ARNERFLAVAGSPLSILEIATVLRTRLGNAGRRAPRRELPNWLARIVALAVPQLRDVKPL
CCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
LGLRRTASSDKARRLLGWNPRPNDEIIAATAESLLRLGLVRP
HHHHHCCCCHHHHHHHCCCCCCCCCEEHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SEVLVTGGSGFIATHVILQLLAAGHRVRTTLRTPDRQSEVLAALARGGATDPHLLSFHA
CCEEEECCCCHHHHHHHHHHHHCCHHHEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEH
ADLTRDDGWAAAVSGCDYVLHIASPLPDHVPDDENELIVPARDGTLRVLRAARDAGVTRV
HHCCCCCCCHHHHCCCCEEEEECCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCEEE
VLTSSFSAVAYGHRPRAKPFDEADWTDLDGPDVQPYPKSKTLAERAAWAFLASEGGALEL
EEECCCHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEECCCCCEEH
ATICPVAVLGPVLGPDFSPSIALVQAMLQGRAPAAPRIHLGLVDVRDVADLHLRAMTDPA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHCCCC
ARNERFLAVAGSPLSILEIATVLRTRLGNAGRRAPRRELPNWLARIVALAVPQLRDVKPL
CCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
LGLRRTASSDKARRLLGWNPRPNDEIIAATAESLLRLGLVRP
HHHHHCCCCHHHHHHHCCCCCCCCCEEHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]