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The map label for this gene is cobS [H]

Identifier: 146341797

GI number: 146341797

Start: 5116473

End: 5117192

Strand: Reverse

Name: cobS [H]

Synonym: BRADO4916

Alternate gene names: 146341797

Gene position: 5117192-5116473 (Counterclockwise)

Preceding gene: 146341798

Following gene: 146341795

Centisome position: 68.63

GC content: 66.25

Gene sequence:

>720_bases
ATGAGCCTGCGTGAATTCTGGATCGCTCTGCAGTTCCTGACGCGTCTGCCGACACCGCGTGTGGCGGAGTCCCGGCCCGA
CGATCTCGCGCGGGCGTCGGTATGGTTTCCTGCCGTGGGGCTGGTGATCGGCGCCTGTCTTTCTGTCATCGCCCGGGTGT
TTCAAAGCGCTGATCCGTGGATCGTCGCGCTCGCCGTCCTGATCGCCTGGATCTGGATCACCGGCGCGCTGCATCTCGAC
GGGCTCGGCGATGTTGCCGATGCGTTCGGTGCCTCGCATGGCAAGCCTGAACGCTTCGTCGCGGTGCTCGGCGACCCCCA
TGCCGGCAGCTTCGCCGTGGTGGCGATCGCCTTACAGATTGCTGCGAAGCTGGTGCTGATCGCAGAGTTGGCCACGGGGC
CGACGGCCTGGGCGCTCGTGCTGATCCCGGCCTGGGCGCGGTTGGGCACACTGGTCTGCACCAAGACGCTGCTGTCGCTG
AAGCCGGGGCTCGGTGCGAGCGTTGCGAACAGCGTCGGCTGGAACTGGATTGTCGGATGGGGCGGCGTTCTCGCCATCGG
CGCGCTCGCCGGTGCAGTCCCGACTTTGATAGCCATCGTGCTGATTCCGGTCATTGCCTTCTACTGGCAATGGCGGCTCG
GCGGCATCACCGGCGACTGTCATGGCGCCAGCATCGAGGTGACGGAGACGCTGCTGCTCGCAGCTTGCGTGCTCGGCTAG

Upstream 100 bases:

>100_bases
TCTGGAGCTTGCGCATTCATCCCGGCACCCGTCTGACGCTCGAGGCTGGGCACGGCCCGGATGGCGCGCCGTGGGCCGAA
ATCGTCGAGATCATCCAGCC

Downstream 100 bases:

>100_bases
CCGGCAAGCGCCTGCGTGAGCCGCTGCCACTGAGCTTCATCGCCCGGCAGGCCGAAACGCAGCCAGCTCGGTTGCCCCGG
GAAGCGGCGGACGTGAATGC

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MSLREFWIALQFLTRLPTPRVAESRPDDLARASVWFPAVGLVIGACLSVIARVFQSADPWIVALAVLIAWIWITGALHLD
GLGDVADAFGASHGKPERFVAVLGDPHAGSFAVVAIALQIAAKLVLIAELATGPTAWALVLIPAWARLGTLVCTKTLLSL
KPGLGASVANSVGWNWIVGWGGVLAIGALAGAVPTLIAIVLIPVIAFYWQWRLGGITGDCHGASIEVTETLLLAACVLG

Sequences:

>Translated_239_residues
MSLREFWIALQFLTRLPTPRVAESRPDDLARASVWFPAVGLVIGACLSVIARVFQSADPWIVALAVLIAWIWITGALHLD
GLGDVADAFGASHGKPERFVAVLGDPHAGSFAVVAIALQIAAKLVLIAELATGPTAWALVLIPAWARLGTLVCTKTLLSL
KPGLGASVANSVGWNWIVGWGGVLAIGALAGAVPTLIAIVLIPVIAFYWQWRLGGITGDCHGASIEVTETLLLAACVLG
>Mature_238_residues
SLREFWIALQFLTRLPTPRVAESRPDDLARASVWFPAVGLVIGACLSVIARVFQSADPWIVALAVLIAWIWITGALHLDG
LGDVADAFGASHGKPERFVAVLGDPHAGSFAVVAIALQIAAKLVLIAELATGPTAWALVLIPAWARLGTLVCTKTLLSLK
PGLGASVANSVGWNWIVGWGGVLAIGALAGAVPTLIAIVLIPVIAFYWQWRLGGITGDCHGASIEVTETLLLAACVLG

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=242, Percent_Identity=28.099173553719, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 24994; Mature: 24863

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLREFWIALQFLTRLPTPRVAESRPDDLARASVWFPAVGLVIGACLSVIARVFQSADPW
CCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
IVALAVLIAWIWITGALHLDGLGDVADAFGASHGKPERFVAVLGDPHAGSFAVVAIALQI
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHH
AAKLVLIAELATGPTAWALVLIPAWARLGTLVCTKTLLSLKPGLGASVANSVGWNWIVGW
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEHH
GGVLAIGALAGAVPTLIAIVLIPVIAFYWQWRLGGITGDCHGASIEVTETLLLAACVLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLREFWIALQFLTRLPTPRVAESRPDDLARASVWFPAVGLVIGACLSVIARVFQSADPW
CHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
IVALAVLIAWIWITGALHLDGLGDVADAFGASHGKPERFVAVLGDPHAGSFAVVAIALQI
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHH
AAKLVLIAELATGPTAWALVLIPAWARLGTLVCTKTLLSLKPGLGASVANSVGWNWIVGW
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEHH
GGVLAIGALAGAVPTLIAIVLIPVIAFYWQWRLGGITGDCHGASIEVTETLLLAACVLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA