Click here to switch to the map view.

The map label for this gene is cobD [H]

Identifier: 146341795

GI number: 146341795

Start: 5114460

End: 5115431

Strand: Reverse

Name: cobD [H]

Synonym: BRADO4914

Alternate gene names: 146341795

Gene position: 5115431-5114460 (Counterclockwise)

Preceding gene: 146341797

Following gene: 146341791

Centisome position: 68.6

GC content: 68.11

Gene sequence:

>972_bases
ATGTTCGTCTCACTCGCGCTCACGGCGCTCCTCTTCGAGGCGGCGGTCGGCTATCCCCAAATGCTGGTGCAGCGGATCGG
TCATCCCGTGATGTGGATGGGCGCGCTGATAGCATGGGCTGATGATCGCTGGAACAGGGCAGGCAACAGCTTCGCGCTGC
GCCAGGCGTTCGGCGTCGCGCTCGTCGTCCTGCTCGTGGCCGGAATGGCGGCCGTCGGCGGGGTTGCGACCTGGCTGCTG
GCGGCGGCTCTCGGCGACATCGCCGCGACGCTCATTCTCGGCGTGGCGGCGAGCAGCCTGCTGGCGCAGCGCAGTCTCGA
CACGCATGTCGCAGCCGTCGCGGAGGGATTGGAGACGAGCGGGCTCGAGGGTGGCCGTCGCGCCGTGGCGATGATCGTCG
GGCGCGATCCCGAGCGGCTCGACGAAGCCGGCGTGTCGCGCGCGGCGATCGAAAGTCTCGCCGAGAATTTCTCCGACGGT
GTCGTGGCGCCATTGTTCTGGCTGTTCATGGGCGGGCTTCCCGGCGTCCTGATCTACAAGGCGGTGAACACCGCCGACAG
CATGATCGGCCATCTCTCCGATCGCCACCGCGCGTTCGGCTGGGCGGCGGCGCGGCTCGATGATCTGATCAACCTGCCGG
CCTCGCGCCTATCCGCCGTGTGGATTGCAGCAGCTGCGCGCGTGGTTCCCGGTGCATCCTCTTCGTCGGCGCTGCATGCC
GTGGCGCGCGATGCTTCGAACCATCGCTCGCCCAATGCCGGCTGGCCGGAGGCCGCGATGGCCGGCGCGTTGGGGCTGCG
TCTGGCCGGGCCGCGCGTCTATCACGGGCACCTCGTCACAGATGCCTGGATGGGCGACGGACGCGCCGATCTGAACGCCT
CCGATATCCGTCGCGCCCTCCAGCTCTACCGGTTCGCTTGTGTGATCCAGGCGCTGTTCGTTGCGATCATCTTCCTCGTC
AAGGTGCTGTAG

Upstream 100 bases:

>100_bases
GCTGATCGCGTCGAGATCGCCGCCGTGAACGATGGCTTCCGGCTGATCCACGCGCGTGCTAGTCGCTCTGTCGTCCATGT
CCATCCCTTAGCGGAGATCG

Downstream 100 bases:

>100_bases
GACGCGGCGTCGGCCTACGCGCCAGCGCAAGAATTCCGCTGACGTTGACGTGGCGCTCGAGATGCGCCGCAAGCCCATCC
AGGGTCGCCTCGACATCGCT

Product: cobalamin biosynthetic protein CobD

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MFVSLALTALLFEAAVGYPQMLVQRIGHPVMWMGALIAWADDRWNRAGNSFALRQAFGVALVVLLVAGMAAVGGVATWLL
AAALGDIAATLILGVAASSLLAQRSLDTHVAAVAEGLETSGLEGGRRAVAMIVGRDPERLDEAGVSRAAIESLAENFSDG
VVAPLFWLFMGGLPGVLIYKAVNTADSMIGHLSDRHRAFGWAAARLDDLINLPASRLSAVWIAAAARVVPGASSSSALHA
VARDASNHRSPNAGWPEAAMAGALGLRLAGPRVYHGHLVTDAWMGDGRADLNASDIRRALQLYRFACVIQALFVAIIFLV
KVL

Sequences:

>Translated_323_residues
MFVSLALTALLFEAAVGYPQMLVQRIGHPVMWMGALIAWADDRWNRAGNSFALRQAFGVALVVLLVAGMAAVGGVATWLL
AAALGDIAATLILGVAASSLLAQRSLDTHVAAVAEGLETSGLEGGRRAVAMIVGRDPERLDEAGVSRAAIESLAENFSDG
VVAPLFWLFMGGLPGVLIYKAVNTADSMIGHLSDRHRAFGWAAARLDDLINLPASRLSAVWIAAAARVVPGASSSSALHA
VARDASNHRSPNAGWPEAAMAGALGLRLAGPRVYHGHLVTDAWMGDGRADLNASDIRRALQLYRFACVIQALFVAIIFLV
KVL
>Mature_323_residues
MFVSLALTALLFEAAVGYPQMLVQRIGHPVMWMGALIAWADDRWNRAGNSFALRQAFGVALVVLLVAGMAAVGGVATWLL
AAALGDIAATLILGVAASSLLAQRSLDTHVAAVAEGLETSGLEGGRRAVAMIVGRDPERLDEAGVSRAAIESLAENFSDG
VVAPLFWLFMGGLPGVLIYKAVNTADSMIGHLSDRHRAFGWAAARLDDLINLPASRLSAVWIAAAARVVPGASSSSALHA
VARDASNHRSPNAGWPEAAMAGALGLRLAGPRVYHGHLVTDAWMGDGRADLNASDIRRALQLYRFACVIQALFVAIIFLV
KVL

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 33933; Mature: 33933

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVSLALTALLFEAAVGYPQMLVQRIGHPVMWMGALIAWADDRWNRAGNSFALRQAFGVA
CHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LVVLLVAGMAAVGGVATWLLAAALGDIAATLILGVAASSLLAQRSLDTHVAAVAEGLETS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLEGGRRAVAMIVGRDPERLDEAGVSRAAIESLAENFSDGVVAPLFWLFMGGLPGVLIYK
CCCCCCCEEEEEECCCHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHH
AVNTADSMIGHLSDRHRAFGWAAARLDDLINLPASRLSAVWIAAAARVVPGASSSSALHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHH
VARDASNHRSPNAGWPEAAMAGALGLRLAGPRVYHGHLVTDAWMGDGRADLNASDIRRAL
HHHHHHCCCCCCCCCCHHHHHHHHHHEECCCEEEECCEEEHHHCCCCCCCCCHHHHHHHH
QLYRFACVIQALFVAIIFLVKVL
HHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MFVSLALTALLFEAAVGYPQMLVQRIGHPVMWMGALIAWADDRWNRAGNSFALRQAFGVA
CHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LVVLLVAGMAAVGGVATWLLAAALGDIAATLILGVAASSLLAQRSLDTHVAAVAEGLETS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLEGGRRAVAMIVGRDPERLDEAGVSRAAIESLAENFSDGVVAPLFWLFMGGLPGVLIYK
CCCCCCCEEEEEECCCHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHH
AVNTADSMIGHLSDRHRAFGWAAARLDDLINLPASRLSAVWIAAAARVVPGASSSSALHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHH
VARDASNHRSPNAGWPEAAMAGALGLRLAGPRVYHGHLVTDAWMGDGRADLNASDIRRAL
HHHHHHCCCCCCCCCCHHHHHHHHHHEECCCEEEECCEEEHHHCCCCCCCCCHHHHHHHH
QLYRFACVIQALFVAIIFLVKVL
HHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7635831; 9256491 [H]