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The map label for this gene is wcaJ [H]

Identifier: 146341159

GI number: 146341159

Start: 4432530

End: 4434083

Strand: Direct

Name: wcaJ [H]

Synonym: BRADO4231

Alternate gene names: 146341159

Gene position: 4432530-4434083 (Clockwise)

Preceding gene: 146341158

Following gene: 146341160

Centisome position: 59.44

GC content: 62.68

Gene sequence:

>1554_bases
GTGGAACCGATCAACGCTCGCTCGATGCTGGATGCTGCCGCGGCCTCGGCCGCGTCGACCGTCGGAGCCGGACAGCCGGC
CATTGAAAGGCGTCGCCGCCTGTCGCCTGCTGCGCTGGCTGTCGCCAATCAGAAGGTTCACCGCGCTTACTCGCCAATCG
TTCTCGCCGGCATTGTGCGTATCACGGATTTTGTGCTGCTGAGCATCGTCGGCAGTGCGATCTATTTCGGCTATGTCGTG
CCGCTCAGCGGCTTTCATTGGGAGTATCTCGTCGCGATCCTCGGTCTCGCCGTGAGCACCGTCGTCTGCTTCCAGGCCGC
GGACATCTATCAGCTCCAGGTCTTCCGAGCCCAGGTGCGGCAGATGACCCGGATGATCTCCTCCTACTGCTTCGTGTTCC
TGCTGTTCATCGGCCTCTCGTTCTTTGCCAAGATCGGCAGCGAAGTCTCGCGCCTGTGGCTTGCGGCCTTCTTCTTCGTC
GGGCTTGGCGCGCTGATCGCGAGCCGCGTCGTGCTGCGCAACCTCATTCGCAGCTGGGCACGCCAGGGCCGCCTCGACCG
CCGGACCATCATCGTCGGCGCCGACCAGAGCGGCGAGGATCTGGTGCGAGCGCTGCGGGCGCAGGACGATTCCGAGATCG
AGATCCTCGGCGTGTTCGATGATCGCAACGACTCGCGCGCGCTCGATACCTGCGCCGGCGCACCGAAGCTCGGCAAGGTC
GACGACATCGTCGAGTTCGCCCGCCGGACCCGCGTCGACCTCGTGCTGTTCGCGCTGCCGATCTCGGCCGAGACCCGCAT
CCTCGACATGCTGAAGAAGCTGTGGGTGCTGCCGGTCGATATCCGCCTCTCGGCCCACACCAACAAGCTCCGCTTCCGTC
CTCGCTCCTATTCCTATCTCGGCGCGGTCCCCACGCTCGACGTGTTCGAAGCTCCGATCACCGATTGGGATCTGGTGATG
AAACAGCTGTTCGATCGACTGGTCGGCAGCCTGATCCTGCTGCTTGCGCTTCCGGTCATGGCGCTCGTGGCACTCGCGAT
CAAGCTCGACAGCCCCGGCCCGGTGCTGTTCCGGCAGAAGCGGTTCGGCTTCAACAACGAGCGGATCGACGTCTTCAAGT
TTCGCTCCCTCTATCACCATCAGGCCGATCCGAACGCATCCAAGGTGGTGACCAAGAATGATCCGCGCGTCACCCGCGTC
GGCCGCTTCATCCGCAAGACCAGCCTCGACGAGTTGCCGCAACTCTTCAACGTCGTCTTCAAGGGCAATCTCTCCCTCGT
CGGACCCAGACCACATGCCGTGCAGGGCAAGCTGCAGAACCGGATGTTCGACGAGGCCGTCGACGGCTACTTTGCCCGCC
ATCGTGTCAAACCCGGGATCACCGGCTGGGCGCAGATCAATGGTTGGCGCGGCGAGATCGACAACGAGGAAAAGATCCAG
AAGCGCGTCGAATTGGACCTCTATTACATCGAGAACTGGTCCGTGCTGTTCGACCTCTACATCCTGTTGAAGACGCCACT
GGCGCTGATGACCAGGAGCGAGAACGCTTACTGA

Upstream 100 bases:

>100_bases
CGATTTGCCGCCTAATCCCGTCGGTAGGGAATTTCCCCCGGCGCTGTCTGCGCTTACATGATCATACATACTAACAAACA
TAATCCGGGCTTGTAGAACT

Downstream 100 bases:

>100_bases
TGTCAGGGATCCGGAGCGAGCATCAGCCCGCTTCGTGCGTGTGATTGGCGGACCCCGCCAATCGTTGCGTATGAGTGTGA
GTGTGAGTTCGTAATGGCGT

Product: putative sugar transferase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MEPINARSMLDAAAASAASTVGAGQPAIERRRRLSPAALAVANQKVHRAYSPIVLAGIVRITDFVLLSIVGSAIYFGYVV
PLSGFHWEYLVAILGLAVSTVVCFQAADIYQLQVFRAQVRQMTRMISSYCFVFLLFIGLSFFAKIGSEVSRLWLAAFFFV
GLGALIASRVVLRNLIRSWARQGRLDRRTIIVGADQSGEDLVRALRAQDDSEIEILGVFDDRNDSRALDTCAGAPKLGKV
DDIVEFARRTRVDLVLFALPISAETRILDMLKKLWVLPVDIRLSAHTNKLRFRPRSYSYLGAVPTLDVFEAPITDWDLVM
KQLFDRLVGSLILLLALPVMALVALAIKLDSPGPVLFRQKRFGFNNERIDVFKFRSLYHHQADPNASKVVTKNDPRVTRV
GRFIRKTSLDELPQLFNVVFKGNLSLVGPRPHAVQGKLQNRMFDEAVDGYFARHRVKPGITGWAQINGWRGEIDNEEKIQ
KRVELDLYYIENWSVLFDLYILLKTPLALMTRSENAY

Sequences:

>Translated_517_residues
MEPINARSMLDAAAASAASTVGAGQPAIERRRRLSPAALAVANQKVHRAYSPIVLAGIVRITDFVLLSIVGSAIYFGYVV
PLSGFHWEYLVAILGLAVSTVVCFQAADIYQLQVFRAQVRQMTRMISSYCFVFLLFIGLSFFAKIGSEVSRLWLAAFFFV
GLGALIASRVVLRNLIRSWARQGRLDRRTIIVGADQSGEDLVRALRAQDDSEIEILGVFDDRNDSRALDTCAGAPKLGKV
DDIVEFARRTRVDLVLFALPISAETRILDMLKKLWVLPVDIRLSAHTNKLRFRPRSYSYLGAVPTLDVFEAPITDWDLVM
KQLFDRLVGSLILLLALPVMALVALAIKLDSPGPVLFRQKRFGFNNERIDVFKFRSLYHHQADPNASKVVTKNDPRVTRV
GRFIRKTSLDELPQLFNVVFKGNLSLVGPRPHAVQGKLQNRMFDEAVDGYFARHRVKPGITGWAQINGWRGEIDNEEKIQ
KRVELDLYYIENWSVLFDLYILLKTPLALMTRSENAY
>Mature_517_residues
MEPINARSMLDAAAASAASTVGAGQPAIERRRRLSPAALAVANQKVHRAYSPIVLAGIVRITDFVLLSIVGSAIYFGYVV
PLSGFHWEYLVAILGLAVSTVVCFQAADIYQLQVFRAQVRQMTRMISSYCFVFLLFIGLSFFAKIGSEVSRLWLAAFFFV
GLGALIASRVVLRNLIRSWARQGRLDRRTIIVGADQSGEDLVRALRAQDDSEIEILGVFDDRNDSRALDTCAGAPKLGKV
DDIVEFARRTRVDLVLFALPISAETRILDMLKKLWVLPVDIRLSAHTNKLRFRPRSYSYLGAVPTLDVFEAPITDWDLVM
KQLFDRLVGSLILLLALPVMALVALAIKLDSPGPVLFRQKRFGFNNERIDVFKFRSLYHHQADPNASKVVTKNDPRVTRV
GRFIRKTSLDELPQLFNVVFKGNLSLVGPRPHAVQGKLQNRMFDEAVDGYFARHRVKPGITGWAQINGWRGEIDNEEKIQ
KRVELDLYYIENWSVLFDLYILLKTPLALMTRSENAY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=435, Percent_Identity=34.7126436781609, Blast_Score=249, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 58188; Mature: 58188

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPINARSMLDAAAASAASTVGAGQPAIERRRRLSPAALAVANQKVHRAYSPIVLAGIVR
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHH
ITDFVLLSIVGSAIYFGYVVPLSGFHWEYLVAILGLAVSTVVCFQAADIYQLQVFRAQVR
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
QMTRMISSYCFVFLLFIGLSFFAKIGSEVSRLWLAAFFFVGLGALIASRVVLRNLIRSWA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQGRLDRRTIIVGADQSGEDLVRALRAQDDSEIEILGVFDDRNDSRALDTCAGAPKLGKV
HCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCH
DDIVEFARRTRVDLVLFALPISAETRILDMLKKLWVLPVDIRLSAHTNKLRFRPRSYSYL
HHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHEECEEEEEECCCCCEEECCCCCCHH
GAVPTLDVFEAPITDWDLVMKQLFDRLVGSLILLLALPVMALVALAIKLDSPGPVLFRQK
CCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHH
RFGFNNERIDVFKFRSLYHHQADPNASKVVTKNDPRVTRVGRFIRKTSLDELPQLFNVVF
HCCCCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHH
KGNLSLVGPRPHAVQGKLQNRMFDEAVDGYFARHRVKPGITGWAQINGWRGEIDNEEKIQ
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHH
KRVELDLYYIENWSVLFDLYILLKTPLALMTRSENAY
HHHHEEEEEECCHHHHHHHHHHHHCCHHHHHCCCCCC
>Mature Secondary Structure
MEPINARSMLDAAAASAASTVGAGQPAIERRRRLSPAALAVANQKVHRAYSPIVLAGIVR
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHH
ITDFVLLSIVGSAIYFGYVVPLSGFHWEYLVAILGLAVSTVVCFQAADIYQLQVFRAQVR
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
QMTRMISSYCFVFLLFIGLSFFAKIGSEVSRLWLAAFFFVGLGALIASRVVLRNLIRSWA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQGRLDRRTIIVGADQSGEDLVRALRAQDDSEIEILGVFDDRNDSRALDTCAGAPKLGKV
HCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCH
DDIVEFARRTRVDLVLFALPISAETRILDMLKKLWVLPVDIRLSAHTNKLRFRPRSYSYL
HHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHEECEEEEEECCCCCEEECCCCCCHH
GAVPTLDVFEAPITDWDLVMKQLFDRLVGSLILLLALPVMALVALAIKLDSPGPVLFRQK
CCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHH
RFGFNNERIDVFKFRSLYHHQADPNASKVVTKNDPRVTRVGRFIRKTSLDELPQLFNVVF
HCCCCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHH
KGNLSLVGPRPHAVQGKLQNRMFDEAVDGYFARHRVKPGITGWAQINGWRGEIDNEEKIQ
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHH
KRVELDLYYIENWSVLFDLYILLKTPLALMTRSENAY
HHHHEEEEEECCHHHHHHHHHHHHCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]