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The map label for this gene is wcaL [C]

Identifier: 146341158

GI number: 146341158

Start: 4431267

End: 4432400

Strand: Direct

Name: wcaL [C]

Synonym: BRADO4230

Alternate gene names: 146341158

Gene position: 4431267-4432400 (Clockwise)

Preceding gene: 146341156

Following gene: 146341159

Centisome position: 59.43

GC content: 67.46

Gene sequence:

>1134_bases
ATGGCGCGCTCGCCGGATAGGCCGTTGAAGATCCTCCACGCAGTCCGAGCTCCGGTCGGCGGCATCTTTCGGCACATTCT
CGACCTCGCCAATGGTCAAGTCGATCGCGGGCACGACGTCGGCATCGTGGCGGACAGCCTGACGGGCGGCGAGCGCGCCG
ATGCGGCACTGGCGAAAATTGCGCCGCGCCTGAAGCTCGGTGTTCATCGCATTGCCATTCACCGCGAGCCGTGGCCAACG
GACGTCATCGCCATCAGCCAATTCCAGCGTCTGTCGCGCCGGCTCGAACCGGACGTTCTGCACGGTCACGGCGCCAAGGC
CGGCGCGTTCGTTCGCCTGCTCCGGCATCGGCCCGGCACCCTTCGGATCTACACGCCTCATGGCGGATCCCTGCACTATC
CGCGGACTACGCTGAAGGGGGCGCTGTATGGGCAGCTCGAACGTGCGCTGATGAACCGCACCGACCTCTTCCTGTTCGAG
AGCGTGTTCGCCCGCGACACCTATGAGCGCATGATCGGCCATCCGCAGGGCCTCGTGCATTGCGTCTTCAACGGTGTGAC
CGCCGGTGAATTCGACCCGGTCCCGCCGGCTGCGGATCAGACCGATCTCGCCTATGTCGGCGAGTTCCGGCACATCAAGG
GCGCCGACCTCCTGATCGACGCCGTCGCGCAGCTGCGCGCCAAGGGCCGGCCGCTGACGCTCACGCTCGGCGGCGACGGC
GAGGAAATGAAGCGCCTCCAGGCTCAGGTCGAGCGGCTCGGACTGACATCCGCGGTGCGCTTCATCGGCCACGTGAGGGC
CCGCGACGGCTTCGCCCAGGGGCGCCTGCTGGTCGTTCCGTCGCGCGGCGACTCCATGCCCTACGTCGTGATCGAGGCCG
GTGCCGCCGGTGTCCCGATGATCGCGGCAAGGGTCGGCGGCATTCCCGAGATCTTCGGGCCGCAGAGCGAGTCGCTGTTC
ATGCCCGGCAACGCCGCCGCAATGGCCGACGCGATCGCGGCGGCCCTCGACGACCCCGCTACCGCGCAGCTGCGAGCAAA
GGCGCTGCGCGAGCGCGTCTTCCAGCATTTTTCGCAGAAAGCCATGGTCGAGGGCGTGCTCGACAGCTACCGTGAAGCGT
TTACCAAGACCTGA

Upstream 100 bases:

>100_bases
AGAGATGCCCGTGCTCCCTCAATCTGCCAGTCGTCAGATCAGCTCGCGCACCAACGCCTGGCACGATCAGGCGCGAGCCG
GTGCCGACGACGAGCCCGGC

Downstream 100 bases:

>100_bases
CCTCATTTACCATCGCTAACCGTTTCTTCCGATTTGCCGCCTAATCCCGTCGGTAGGGAATTTCCCCCGGCGCTGTCTGC
GCTTACATGATCATACATAC

Product: putative glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MARSPDRPLKILHAVRAPVGGIFRHILDLANGQVDRGHDVGIVADSLTGGERADAALAKIAPRLKLGVHRIAIHREPWPT
DVIAISQFQRLSRRLEPDVLHGHGAKAGAFVRLLRHRPGTLRIYTPHGGSLHYPRTTLKGALYGQLERALMNRTDLFLFE
SVFARDTYERMIGHPQGLVHCVFNGVTAGEFDPVPPAADQTDLAYVGEFRHIKGADLLIDAVAQLRAKGRPLTLTLGGDG
EEMKRLQAQVERLGLTSAVRFIGHVRARDGFAQGRLLVVPSRGDSMPYVVIEAGAAGVPMIAARVGGIPEIFGPQSESLF
MPGNAAAMADAIAAALDDPATAQLRAKALRERVFQHFSQKAMVEGVLDSYREAFTKT

Sequences:

>Translated_377_residues
MARSPDRPLKILHAVRAPVGGIFRHILDLANGQVDRGHDVGIVADSLTGGERADAALAKIAPRLKLGVHRIAIHREPWPT
DVIAISQFQRLSRRLEPDVLHGHGAKAGAFVRLLRHRPGTLRIYTPHGGSLHYPRTTLKGALYGQLERALMNRTDLFLFE
SVFARDTYERMIGHPQGLVHCVFNGVTAGEFDPVPPAADQTDLAYVGEFRHIKGADLLIDAVAQLRAKGRPLTLTLGGDG
EEMKRLQAQVERLGLTSAVRFIGHVRARDGFAQGRLLVVPSRGDSMPYVVIEAGAAGVPMIAARVGGIPEIFGPQSESLF
MPGNAAAMADAIAAALDDPATAQLRAKALRERVFQHFSQKAMVEGVLDSYREAFTKT
>Mature_376_residues
ARSPDRPLKILHAVRAPVGGIFRHILDLANGQVDRGHDVGIVADSLTGGERADAALAKIAPRLKLGVHRIAIHREPWPTD
VIAISQFQRLSRRLEPDVLHGHGAKAGAFVRLLRHRPGTLRIYTPHGGSLHYPRTTLKGALYGQLERALMNRTDLFLFES
VFARDTYERMIGHPQGLVHCVFNGVTAGEFDPVPPAADQTDLAYVGEFRHIKGADLLIDAVAQLRAKGRPLTLTLGGDGE
EMKRLQAQVERLGLTSAVRFIGHVRARDGFAQGRLLVVPSRGDSMPYVVIEAGAAGVPMIAARVGGIPEIFGPQSESLFM
PGNAAAMADAIAAALDDPATAQLRAKALRERVFQHFSQKAMVEGVLDSYREAFTKT

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Homo sapiens, GI11863130, Length=325, Percent_Identity=22.7692307692308, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17532709, Length=117, Percent_Identity=28.2051282051282, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI9755344, Length=365, Percent_Identity=22.4657534246575, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR013534 [H]

Pfam domain/function: PF08323 Glyco_transf_5; PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 40929; Mature: 40798

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSPDRPLKILHAVRAPVGGIFRHILDLANGQVDRGHDVGIVADSLTGGERADAALAKI
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHH
APRLKLGVHRIAIHREPWPTDVIAISQFQRLSRRLEPDVLHGHGAKAGAFVRLLRHRPGT
HHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCE
LRIYTPHGGSLHYPRTTLKGALYGQLERALMNRTDLFLFESVFARDTYERMIGHPQGLVH
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
CVFNGVTAGEFDPVPPAADQTDLAYVGEFRHIKGADLLIDAVAQLRAKGRPLTLTLGGDG
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCCH
EEMKRLQAQVERLGLTSAVRFIGHVRARDGFAQGRLLVVPSRGDSMPYVVIEAGAAGVPM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCCCCHH
IAARVGGIPEIFGPQSESLFMPGNAAAMADAIAAALDDPATAQLRAKALRERVFQHFSQK
HHHHHCCCCHHCCCCCCCEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AMVEGVLDSYREAFTKT
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARSPDRPLKILHAVRAPVGGIFRHILDLANGQVDRGHDVGIVADSLTGGERADAALAKI
CCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHH
APRLKLGVHRIAIHREPWPTDVIAISQFQRLSRRLEPDVLHGHGAKAGAFVRLLRHRPGT
HHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCE
LRIYTPHGGSLHYPRTTLKGALYGQLERALMNRTDLFLFESVFARDTYERMIGHPQGLVH
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
CVFNGVTAGEFDPVPPAADQTDLAYVGEFRHIKGADLLIDAVAQLRAKGRPLTLTLGGDG
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCCH
EEMKRLQAQVERLGLTSAVRFIGHVRARDGFAQGRLLVVPSRGDSMPYVVIEAGAAGVPM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCCCCHH
IAARVGGIPEIFGPQSESLFMPGNAAAMADAIAAALDDPATAQLRAKALRERVFQHFSQK
HHHHHCCCCHHCCCCCCCEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AMVEGVLDSYREAFTKT
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]