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The map label for this gene is braE [H]

Identifier: 146341003

GI number: 146341003

Start: 4262235

End: 4263287

Strand: Reverse

Name: braE [H]

Synonym: BRADO4074

Alternate gene names: 146341003

Gene position: 4263287-4262235 (Counterclockwise)

Preceding gene: 146341004

Following gene: 146341002

Centisome position: 57.17

GC content: 64.67

Gene sequence:

>1053_bases
ATGGACAAGCGCCTTCGTCTCGCGCTCCAGGCCGCCGGAACCGTCGTTGCCGTCGGCGTCATTGCGATCGTGCCGAGCGT
GATCGAGCTGTTCGGCCTGATGCAGCTGACCTTGTTCGCCGCGATGTCGGTGCTGGCCTTGAGCCTCGCCTTCATCTGGG
GCTATGGCGGCATTCTCTCCTTCGGCCAGACCGCCTTCTTCGGCCTCGGCGGCTACGCCTATGCCATTGCCGCCGTGAAC
TTTCAGGACTCGACCCCGGCGATCCTGCTGGCGATCGTCGTCCCCGCGCTGTTCGCGGCCATCCTCGGCTATTTCATCTT
CTTCGGCCGCATCTCCGACGTCTATCTCGGCGTCATCACGCTGACTGTCACTTTGATCCTGTTCAACTCGGTCAACTCGA
CCTCCGGCGACGCCTACAAGATCGGCAAAGCCTTGCTCGGCGGCTTCAACGGCATGCCGGCGGTGCCGACATTCAACGTG
CCGTTCGATCCGTCGACCGTACTGTCGCCGGAACAGTCGTGGTGGACGACGGCCTATGTGCTGCTCGGGGTGTTCCTGCT
GCTGCGGCTCCTGCTGGCGCTGCCCGCCGGGCGCATCATCGTCGCCGTGCGCGAGAACGAAGTTCGCGCGTCCCTGCTCG
GCTACGATCCGCGGCTCGTGAAGTGCCTGACCTTCATCCTCGGCGGCGCCATCGCCGGTCTCGCCGGGGCGCTTTATGTC
AACTGGGGCGCCTTCGTCAGCCCGACCATCTTCAGCCTGTCGCTGTCGGCGGAGATCATCATCTGGATCACGGTCGGCGG
CCTCGGCACCTTGCTCGGGCCGATCATCGGCTGCGTCGTGATCGAATACATCGTCGCCTACATCGGCTCGCAGCAGCTCC
TGAACTCCAATCTCGTGCTCGGCGGCGTGCTGGTGGTGTTCGTGCTCCTGCTGCCGAAGGGCATAGTGCCGACCGCGCGT
GATCTCGTGATGCGGCTGATCCCGGACAGGAAGAAGACCAACGACCAGCCGGTGCAGTCAGCGGCGGCGCCGCATCCCGC
AGGAGCCGAATGA

Upstream 100 bases:

>100_bases
CGCCTGTCTACGGCGAGGTTATCGTCTTCACCACCGCGATCGTGCTCATCCGTCTGCTGCCGCAGGGCATCTCCGGGCGC
TTCTTCAAGGGGAACCTGTG

Downstream 100 bases:

>100_bases
CATGGCGGACCTCGTTCCTCTGCTCAAGGCCGAGCACCTGACGATGCGTTTCGGCGGCGTGGTCGCCAACGACGACATCA
CCTTCACCCTCCAGGAGATG

Product: branched chain amino acid ABC transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MDKRLRLALQAAGTVVAVGVIAIVPSVIELFGLMQLTLFAAMSVLALSLAFIWGYGGILSFGQTAFFGLGGYAYAIAAVN
FQDSTPAILLAIVVPALFAAILGYFIFFGRISDVYLGVITLTVTLILFNSVNSTSGDAYKIGKALLGGFNGMPAVPTFNV
PFDPSTVLSPEQSWWTTAYVLLGVFLLLRLLLALPAGRIIVAVRENEVRASLLGYDPRLVKCLTFILGGAIAGLAGALYV
NWGAFVSPTIFSLSLSAEIIIWITVGGLGTLLGPIIGCVVIEYIVAYIGSQQLLNSNLVLGGVLVVFVLLLPKGIVPTAR
DLVMRLIPDRKKTNDQPVQSAAAPHPAGAE

Sequences:

>Translated_350_residues
MDKRLRLALQAAGTVVAVGVIAIVPSVIELFGLMQLTLFAAMSVLALSLAFIWGYGGILSFGQTAFFGLGGYAYAIAAVN
FQDSTPAILLAIVVPALFAAILGYFIFFGRISDVYLGVITLTVTLILFNSVNSTSGDAYKIGKALLGGFNGMPAVPTFNV
PFDPSTVLSPEQSWWTTAYVLLGVFLLLRLLLALPAGRIIVAVRENEVRASLLGYDPRLVKCLTFILGGAIAGLAGALYV
NWGAFVSPTIFSLSLSAEIIIWITVGGLGTLLGPIIGCVVIEYIVAYIGSQQLLNSNLVLGGVLVVFVLLLPKGIVPTAR
DLVMRLIPDRKKTNDQPVQSAAAPHPAGAE
>Mature_350_residues
MDKRLRLALQAAGTVVAVGVIAIVPSVIELFGLMQLTLFAAMSVLALSLAFIWGYGGILSFGQTAFFGLGGYAYAIAAVN
FQDSTPAILLAIVVPALFAAILGYFIFFGRISDVYLGVITLTVTLILFNSVNSTSGDAYKIGKALLGGFNGMPAVPTFNV
PFDPSTVLSPEQSWWTTAYVLLGVFLLLRLLLALPAGRIIVAVRENEVRASLLGYDPRLVKCLTFILGGAIAGLAGALYV
NWGAFVSPTIFSLSLSAEIIIWITVGGLGTLLGPIIGCVVIEYIVAYIGSQQLLNSNLVLGGVLVVFVLLLPKGIVPTAR
DLVMRLIPDRKKTNDQPVQSAAAPHPAGAE

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=317, Percent_Identity=25.5520504731861, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 36916; Mature: 36916

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKRLRLALQAAGTVVAVGVIAIVPSVIELFGLMQLTLFAAMSVLALSLAFIWGYGGILS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGQTAFFGLGGYAYAIAAVNFQDSTPAILLAIVVPALFAAILGYFIFFGRISDVYLGVIT
HCCHHHHHCCHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTVTLILFNSVNSTSGDAYKIGKALLGGFNGMPAVPTFNVPFDPSTVLSPEQSWWTTAYV
HHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LLGVFLLLRLLLALPAGRIIVAVRENEVRASLLGYDPRLVKCLTFILGGAIAGLAGALYV
HHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
NWGAFVSPTIFSLSLSAEIIIWITVGGLGTLLGPIIGCVVIEYIVAYIGSQQLLNSNLVL
CCCCHHCCHHEEEEECCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHH
GGVLVVFVLLLPKGIVPTARDLVMRLIPDRKKTNDQPVQSAAAPHPAGAE
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCC
>Mature Secondary Structure
MDKRLRLALQAAGTVVAVGVIAIVPSVIELFGLMQLTLFAAMSVLALSLAFIWGYGGILS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGQTAFFGLGGYAYAIAAVNFQDSTPAILLAIVVPALFAAILGYFIFFGRISDVYLGVIT
HCCHHHHHCCHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTVTLILFNSVNSTSGDAYKIGKALLGGFNGMPAVPTFNVPFDPSTVLSPEQSWWTTAYV
HHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LLGVFLLLRLLLALPAGRIIVAVRENEVRASLLGYDPRLVKCLTFILGGAIAGLAGALYV
HHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
NWGAFVSPTIFSLSLSAEIIIWITVGGLGTLLGPIIGCVVIEYIVAYIGSQQLLNSNLVL
CCCCHHCCHHEEEEECCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHH
GGVLVVFVLLLPKGIVPTARDLVMRLIPDRKKTNDQPVQSAAAPHPAGAE
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]