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The map label for this gene is puuB [H]

Identifier: 146340895

GI number: 146340895

Start: 4144179

End: 4145489

Strand: Reverse

Name: puuB [H]

Synonym: BRADO3961

Alternate gene names: 146340895

Gene position: 4145489-4144179 (Counterclockwise)

Preceding gene: 146340897

Following gene: 146340887

Centisome position: 55.59

GC content: 68.19

Gene sequence:

>1311_bases
ATGAGCACGACCACCGATCACGTTCAATGGCCTCCGTCCCTGTGGGCGGCCCAGACCCCACCCGGACCGGCGTTGCCGGA
GCTGCAGGGCAGCCGGCAGGCGGATGTCGTCATCATCGGGGGCGGCTTCACGGGCCTGTCGACGGCGCTTAATTTGCGCG
AGGCTGGGATCGATGTGGCGATCGTCGAGGCGATGGAGCCGGGCTGGGGCGCCTCGGGCCGCAACAACGGTCAGGTGATC
CCGACGCTGTCGCGCCCCGATCCCGAGGATCTCGTTGCCCGCCATGGCGCGGCCGGCGAGCGCTTCGTCGCCATGCTGCG
CGACAGTGCGCAAACGCTGTTCGACACCGTGAGGCGCTACAGCATCGAGGCCGAACACGAGCAGGCTGGTTGGGTGCAGC
CGGTCCATACGCCCGGGCGTATCAAGATCGCCGAGCGCCGGGTCAGGCAGTGGTCGAAGTTCGGCGCTCCGGTCGAGCTG
TTGTCGCGCGATCAGGCGCGCGACATGCTCGGCTCCGACGCCTGGCACGGCGGTTTCTGGAACAAGACCGGCGGGCACAT
CAATCCGCTGGCGCTGGCGCGCGGGCTTGCGCGCACCGTGCTCGCTCTGGGCGGCCGGATCTATGCGCGCTCTCCGGTGA
TCGACTACGCGAGGCGTGGCGATCGCTGGGTCGTCCGCACCGCACGAGGGGAGCTGTCGGCGCGTGCGTTGGTGCTGGCG
AGCAATGCCTATAGCGGCGAGTTCTCCAAGGCGCTCGCCCCCGACATCGCGCATGAGGTGATGCCGGTATTGTCGTGGCA
GATGGCGACGCAGCCGCTGTCGGACAATGTGCGCAAGACCATCATCCCGTCGCGCCAGGCGATGTCGGACACCCATGGCG
AGCTGTATTTCGCACGCTATGACGCGCGCAACCGGCTGGTGACCGGCGGCGCCGTGATCGGTCCCGGCAACAAGGCCGAG
CGGCTGAAGGCGCGCGTCTCCGAACGCCTGCAGCGGTTGTGGCCGCAGATCGGTGACGTCAAGTTCGACTATGTCTGGAA
CGGCTATGTCGGCATGACCACGGACTACATGCCACGCATTCATCGGCTCGGTCCGGATGCCTATGGCTGGACCGGATGCA
ACGGGCGCGCGGTCGCGCTGACGATCCCGCTCGGCGCAGAACTCGCGAAGGCCGTGCAGGGTGTGCCGGCTGACGAGCTG
GCGCTGCCGTTCACCGAGCCGGTCACGATCCCCGCGCACGCGCTGCTGCGGCCGTTCGCGCCGCTGATGCTGCTGCTGTA
TCGCTATCGCGATGCGCGCGAGATCGCCTAG

Upstream 100 bases:

>100_bases
CCAATCGACCCATGCGCCGCAACCGGCGTGGCCGGGCGAGGGATGCAAGGCAAGATCGATCTCCGTGAGGGCGATCGATC
AGCAGAAACGAGCTGCCGCG

Downstream 100 bases:

>100_bases
TCGTCAGCCTTTGGCCTCAGCGATGGAGGTCCGGGCGAAGTCGCCGCCCGGAATGGCCGCGAGCAGGGCCTGCGTGTAGG
AATGCCTGGGGCTGCCGAAC

Product: putative FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MSTTTDHVQWPPSLWAAQTPPGPALPELQGSRQADVVIIGGGFTGLSTALNLREAGIDVAIVEAMEPGWGASGRNNGQVI
PTLSRPDPEDLVARHGAAGERFVAMLRDSAQTLFDTVRRYSIEAEHEQAGWVQPVHTPGRIKIAERRVRQWSKFGAPVEL
LSRDQARDMLGSDAWHGGFWNKTGGHINPLALARGLARTVLALGGRIYARSPVIDYARRGDRWVVRTARGELSARALVLA
SNAYSGEFSKALAPDIAHEVMPVLSWQMATQPLSDNVRKTIIPSRQAMSDTHGELYFARYDARNRLVTGGAVIGPGNKAE
RLKARVSERLQRLWPQIGDVKFDYVWNGYVGMTTDYMPRIHRLGPDAYGWTGCNGRAVALTIPLGAELAKAVQGVPADEL
ALPFTEPVTIPAHALLRPFAPLMLLLYRYRDAREIA

Sequences:

>Translated_436_residues
MSTTTDHVQWPPSLWAAQTPPGPALPELQGSRQADVVIIGGGFTGLSTALNLREAGIDVAIVEAMEPGWGASGRNNGQVI
PTLSRPDPEDLVARHGAAGERFVAMLRDSAQTLFDTVRRYSIEAEHEQAGWVQPVHTPGRIKIAERRVRQWSKFGAPVEL
LSRDQARDMLGSDAWHGGFWNKTGGHINPLALARGLARTVLALGGRIYARSPVIDYARRGDRWVVRTARGELSARALVLA
SNAYSGEFSKALAPDIAHEVMPVLSWQMATQPLSDNVRKTIIPSRQAMSDTHGELYFARYDARNRLVTGGAVIGPGNKAE
RLKARVSERLQRLWPQIGDVKFDYVWNGYVGMTTDYMPRIHRLGPDAYGWTGCNGRAVALTIPLGAELAKAVQGVPADEL
ALPFTEPVTIPAHALLRPFAPLMLLLYRYRDAREIA
>Mature_435_residues
STTTDHVQWPPSLWAAQTPPGPALPELQGSRQADVVIIGGGFTGLSTALNLREAGIDVAIVEAMEPGWGASGRNNGQVIP
TLSRPDPEDLVARHGAAGERFVAMLRDSAQTLFDTVRRYSIEAEHEQAGWVQPVHTPGRIKIAERRVRQWSKFGAPVELL
SRDQARDMLGSDAWHGGFWNKTGGHINPLALARGLARTVLALGGRIYARSPVIDYARRGDRWVVRTARGELSARALVLAS
NAYSGEFSKALAPDIAHEVMPVLSWQMATQPLSDNVRKTIIPSRQAMSDTHGELYFARYDARNRLVTGGAVIGPGNKAER
LKARVSERLQRLWPQIGDVKFDYVWNGYVGMTTDYMPRIHRLGPDAYGWTGCNGRAVALTIPLGAELAKAVQGVPADELA
LPFTEPVTIPAHALLRPFAPLMLLLYRYRDAREIA

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=430, Percent_Identity=31.1627906976744, Blast_Score=204, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47796; Mature: 47665

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTTDHVQWPPSLWAAQTPPGPALPELQGSRQADVVIIGGGFTGLSTALNLREAGIDVA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEE
IVEAMEPGWGASGRNNGQVIPTLSRPDPEDLVARHGAAGERFVAMLRDSAQTLFDTVRRY
EEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
SIEAEHEQAGWVQPVHTPGRIKIAERRVRQWSKFGAPVELLSRDQARDMLGSDAWHGGFW
CCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCC
NKTGGHINPLALARGLARTVLALGGRIYARSPVIDYARRGDRWVVRTARGELSARALVLA
CCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCHHHHHHCCCEEEEEECCCCCCEEEEEEE
SNAYSGEFSKALAPDIAHEVMPVLSWQMATQPLSDNVRKTIIPSRQAMSDTHGELYFARY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHCCCHHHHCCCCCEEEEEEE
DARNRLVTGGAVIGPGNKAERLKARVSERLQRLWPQIGDVKFDYVWNGYVGMTTDYMPRI
CCCCEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCEECCCHHHHHHH
HRLGPDAYGWTGCNGRAVALTIPLGAELAKAVQGVPADELALPFTEPVTIPAHALLRPFA
HHCCCCCCCCCCCCCEEEEEEECCCHHHHHHHCCCCHHHCCCCCCCCCCCCHHHHHHHHH
PLMLLLYRYRDAREIA
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STTTDHVQWPPSLWAAQTPPGPALPELQGSRQADVVIIGGGFTGLSTALNLREAGIDVA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEE
IVEAMEPGWGASGRNNGQVIPTLSRPDPEDLVARHGAAGERFVAMLRDSAQTLFDTVRRY
EEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
SIEAEHEQAGWVQPVHTPGRIKIAERRVRQWSKFGAPVELLSRDQARDMLGSDAWHGGFW
CCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCC
NKTGGHINPLALARGLARTVLALGGRIYARSPVIDYARRGDRWVVRTARGELSARALVLA
CCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCHHHHHHCCCEEEEEECCCCCCEEEEEEE
SNAYSGEFSKALAPDIAHEVMPVLSWQMATQPLSDNVRKTIIPSRQAMSDTHGELYFARY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHCCCHHHHCCCCCEEEEEEE
DARNRLVTGGAVIGPGNKAERLKARVSERLQRLWPQIGDVKFDYVWNGYVGMTTDYMPRI
CCCCEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCEECCCHHHHHHH
HRLGPDAYGWTGCNGRAVALTIPLGAELAKAVQGVPADELALPFTEPVTIPAHALLRPFA
HHCCCCCCCCCCCCCEEEEEEECCCHHHHHHHCCCCHHHCCCCCCCCCCCCHHHHHHHHH
PLMLLLYRYRDAREIA
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]