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The map label for this gene is ntrB [H]

Identifier: 146340808

GI number: 146340808

Start: 4042399

End: 4043574

Strand: Reverse

Name: ntrB [H]

Synonym: BRADO3867

Alternate gene names: 146340808

Gene position: 4043574-4042399 (Counterclockwise)

Preceding gene: 146340809

Following gene: 146340807

Centisome position: 54.23

GC content: 65.73

Gene sequence:

>1176_bases
ATGAGCTCAGCCGCAGAACAGCGCAAGCGCCTTCCGTCCGATGGCGATGCCATCCTCAACGCGTTGCCCAATCCGGTCCT
CGTCATCGGCCCGGACGGCATGATCATGGACGCCAACATGGCGGCGGAATCGTTCTTCGATTCCTCCACGCAGTTCCTGC
AGCGGCAGTCGTTGAAGAAGCTGGTGCCGTTCGGCTCGCCGCTGCTGGCGCTGATCGACCAGGTGCGCACGACCAGCGCG
CCAGTCAACGAGTACAAGGTCGACCTGGGCACGCCGCGCACCGGCGCCGACCGGCTCGTCGATCTTCATGTCGCCCCCTT
GCCGGAGCGGCCGGGGCATATCGTCGTGATGCTGCAGGAGCGCACCATCGCCGACAAGATGGACCGCCAGCTCACCCATC
GTAGCGCCGCGCGCTCGGTGATCGCGCTGGCGGCGATGCTGGCGCATGAGATCAAGAACCCGCTGTCGGGCATCCGTGGC
GCCGCCCAGCTGCTGGAGCAGCAGGCTTCCTCCGAGGACCGCATGCTGACCCGGCTGATCTGCGACGAGGCCGACCGCAT
CGTCACCCTGGTCGACCGCATGGAGGTGTTCGGCGACGACCGCCCGGTGGCGCGCGGGCCGGTCAACATCCATTCCGTGC
TCGATCACGTGAAGCGGCTGGCGCAGTCGGGTTTCGCCCGCAACATCCGCTTCATCGAGGACTACGACCCGTCGCTGCCG
CCGGTGCTCGCCAACCAGGACCAGCTGATCCAGGTGTTCCTGAACCTGGTCAAGAATGCCGCCGAAGCAGTCGTCGATCT
CGGCTCCGACGCCGAAATCCAGCTCACCACGGCCTTCCGCCCGGGCGTGCGGCTGTCGGTGCCGGGCAAGAAGTCGCGCG
TGTCGCTGCCGCTGGAGTTCTGCGTCAGGGACAACGGCCCGGGCGTGCCGGAAGACCTGATGCCGAACCTGTTCGATCCG
TTCGTGACCACCAAGCCGACCGGAAGCGGCCTCGGTCTTGCATTGGTCGCGAAGATCATCGGCGACCATGGCGGGATCAT
CGAGTGCGAATCGCAGCCGCGCAAGACGACCTTCCGGGTGCTGATGCCGATGTACAGCGCGAGCAAATATCCAGACCAAG
GCCATCGCGATGACGTTTCGGGGACGCCGTCGCATGCCTCACAGGGATCAAATTGA

Upstream 100 bases:

>100_bases
TGAAGGCGTGGCGCGAGCGTATCCTGACATCCGAGCAGCCGTCCCAGGTGCATCGGGCGCTGCGTGAGGCCTTTGACGAT
TTTGCCTGGAGTGCAGCCGC

Downstream 100 bases:

>100_bases
GGACCACCTATGCCTGCAGGTAGCATTCTCGTTGCCGACGACGATACGGCCATCCGCACGGTCCTGAACCAGGCGCTGTC
ACGCGCCGGCTACGAGGTGC

Product: nitrogen regulation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MSSAAEQRKRLPSDGDAILNALPNPVLVIGPDGMIMDANMAAESFFDSSTQFLQRQSLKKLVPFGSPLLALIDQVRTTSA
PVNEYKVDLGTPRTGADRLVDLHVAPLPERPGHIVVMLQERTIADKMDRQLTHRSAARSVIALAAMLAHEIKNPLSGIRG
AAQLLEQQASSEDRMLTRLICDEADRIVTLVDRMEVFGDDRPVARGPVNIHSVLDHVKRLAQSGFARNIRFIEDYDPSLP
PVLANQDQLIQVFLNLVKNAAEAVVDLGSDAEIQLTTAFRPGVRLSVPGKKSRVSLPLEFCVRDNGPGVPEDLMPNLFDP
FVTTKPTGSGLGLALVAKIIGDHGGIIECESQPRKTTFRVLMPMYSASKYPDQGHRDDVSGTPSHASQGSN

Sequences:

>Translated_391_residues
MSSAAEQRKRLPSDGDAILNALPNPVLVIGPDGMIMDANMAAESFFDSSTQFLQRQSLKKLVPFGSPLLALIDQVRTTSA
PVNEYKVDLGTPRTGADRLVDLHVAPLPERPGHIVVMLQERTIADKMDRQLTHRSAARSVIALAAMLAHEIKNPLSGIRG
AAQLLEQQASSEDRMLTRLICDEADRIVTLVDRMEVFGDDRPVARGPVNIHSVLDHVKRLAQSGFARNIRFIEDYDPSLP
PVLANQDQLIQVFLNLVKNAAEAVVDLGSDAEIQLTTAFRPGVRLSVPGKKSRVSLPLEFCVRDNGPGVPEDLMPNLFDP
FVTTKPTGSGLGLALVAKIIGDHGGIIECESQPRKTTFRVLMPMYSASKYPDQGHRDDVSGTPSHASQGSN
>Mature_390_residues
SSAAEQRKRLPSDGDAILNALPNPVLVIGPDGMIMDANMAAESFFDSSTQFLQRQSLKKLVPFGSPLLALIDQVRTTSAP
VNEYKVDLGTPRTGADRLVDLHVAPLPERPGHIVVMLQERTIADKMDRQLTHRSAARSVIALAAMLAHEIKNPLSGIRGA
AQLLEQQASSEDRMLTRLICDEADRIVTLVDRMEVFGDDRPVARGPVNIHSVLDHVKRLAQSGFARNIRFIEDYDPSLPP
VLANQDQLIQVFLNLVKNAAEAVVDLGSDAEIQLTTAFRPGVRLSVPGKKSRVSLPLEFCVRDNGPGVPEDLMPNLFDPF
VTTKPTGSGLGLALVAKIIGDHGGIIECESQPRKTTFRVLMPMYSASKYPDQGHRDDVSGTPSHASQGSN

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=358, Percent_Identity=34.0782122905028, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1790436, Length=234, Percent_Identity=32.9059829059829, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1788549, Length=362, Percent_Identity=27.3480662983425, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 42527; Mature: 42396

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSAAEQRKRLPSDGDAILNALPNPVLVIGPDGMIMDANMAAESFFDSSTQFLQRQSLKK
CCCHHHHHHCCCCCHHHHHHHCCCCEEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHH
LVPFGSPLLALIDQVRTTSAPVNEYKVDLGTPRTGADRLVDLHVAPLPERPGHIVVMLQE
HCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHEEEEEECCCCCCCCEEEEEECC
RTIADKMDRQLTHRSAARSVIALAAMLAHEIKNPLSGIRGAAQLLEQQASSEDRMLTRLI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
CDEADRIVTLVDRMEVFGDDRPVARGPVNIHSVLDHVKRLAQSGFARNIRFIEDYDPSLP
HCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCEEECCCCCCC
PVLANQDQLIQVFLNLVKNAAEAVVDLGSDAEIQLTTAFRPGVRLSVPGKKSRVSLPLEF
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEEEECCCCCCEEECCHHE
CVRDNGPGVPEDLMPNLFDPFVTTKPTGSGLGLALVAKIIGDHGGIIECESQPRKTTFRV
EEECCCCCCCHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHH
LMPMYSASKYPDQGHRDDVSGTPSHASQGSN
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSAAEQRKRLPSDGDAILNALPNPVLVIGPDGMIMDANMAAESFFDSSTQFLQRQSLKK
CCHHHHHHCCCCCHHHHHHHCCCCEEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHH
LVPFGSPLLALIDQVRTTSAPVNEYKVDLGTPRTGADRLVDLHVAPLPERPGHIVVMLQE
HCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHEEEEEECCCCCCCCEEEEEECC
RTIADKMDRQLTHRSAARSVIALAAMLAHEIKNPLSGIRGAAQLLEQQASSEDRMLTRLI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
CDEADRIVTLVDRMEVFGDDRPVARGPVNIHSVLDHVKRLAQSGFARNIRFIEDYDPSLP
HCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCEEECCCCCCC
PVLANQDQLIQVFLNLVKNAAEAVVDLGSDAEIQLTTAFRPGVRLSVPGKKSRVSLPLEF
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEEEECCCCCCEEECCHHE
CVRDNGPGVPEDLMPNLFDPFVTTKPTGSGLGLALVAKIIGDHGGIIECESQPRKTTFRV
EEECCCCCCCHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHH
LMPMYSASKYPDQGHRDDVSGTPSHASQGSN
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3020561 [H]