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The map label for this gene is dus [H]

Identifier: 146340809

GI number: 146340809

Start: 4043571

End: 4044524

Strand: Reverse

Name: dus [H]

Synonym: BRADO3868

Alternate gene names: 146340809

Gene position: 4044524-4043571 (Counterclockwise)

Preceding gene: 146340813

Following gene: 146340808

Centisome position: 54.24

GC content: 70.75

Gene sequence:

>954_bases
ATGTCGGGGATCACCGATGCGCCGTTCCGCCGACTCGCGGCCGAGCTGGGCGCAGGCCTGGTCGTGTCCGAGATGACCGC
GAGCGACGATCTCGTCCGCGGGCGGTGGATATCTCGGTTGCGCTGCGAGGCCGGCGGCATCGGCCCGCATGTGGTGCAGC
TCGCCGGCTGTGAGGCGCATTGGATGGCCGAGGGTGCCAAGGTCGCCGCCGATGGCGGCGCCGACATCATCGACATCAAC
ATGGGCTGCCCGGCGCGGCACGTCACCGGCGGCCAGTCCGGCTCAGCCCTGATGCGCGATCTCGATCATGCGCTGACGCT
GATCGAGGCGACGGTCGAGGCCGTCGACGTGCCCGTGACCTTGAAGATGCGGCTCGGCTGGGACGAGCGCAGCCTGAATG
CGCCTGAGCTGGCGCGCCGCGCGGAGGCCGCCGGCGTGCAGCTGATCACCGTGCATGGCCGGACGCGGCAGCAGTTCTAC
AAGGGCGAGGCGGACTGGAGCGCAGTGCGCGCGGTGAAGCAGGCCGTGGACCTGCCTGTCGTCGTCAATGGCGACATCAC
GAGCTACGAGGATGCGCGCGAGGCGCTCGACGCCTCCGGCGCCGATGCTGTGATGATCGGTCGCGGCGCGCAGGGGCAGC
CCTGGCTGCCCGGCCAGATCGGCCGCCGGCTCGACGGCGGCGCCGAGGAGGGCGCGCCGGAGCTGACGCAGCAGCTCGCG
CTGCTGACCGCGCTGTATGACGAGATCTGCCGCCATTATGGCGAGCGGGTCGGCCTGCGCCACGCCCGCAAGCATCTCAG
CTGGGGGCTCGACGTCGCCGCCGCGCACGCCCAGGCGCCGGCCGAAAAACTGAAGGCGTGGCGCGAGCGTATCCTGACAT
CCGAGCAGCCGTCCCAGGTGCATCGGGCGCTGCGTGAGGCCTTTGACGATTTTGCCTGGAGTGCAGCCGCATGA

Upstream 100 bases:

>100_bases
TGAGCTCGAAGAGTCGTTCGTGACCGCTACGGCCAATATACCCGCCAAAGCCTTGAAGATAGGTGACGTTTCGCTCGTCA
ACCGGGCTGTTCTGGCGCCG

Downstream 100 bases:

>100_bases
GCTCAGCCGCAGAACAGCGCAAGCGCCTTCCGTCCGATGGCGATGCCATCCTCAACGCGTTGCCCAATCCGGTCCTCGTC
ATCGGCCCGGACGGCATGAT

Product: putative tRNA-dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MSGITDAPFRRLAAELGAGLVVSEMTASDDLVRGRWISRLRCEAGGIGPHVVQLAGCEAHWMAEGAKVAADGGADIIDIN
MGCPARHVTGGQSGSALMRDLDHALTLIEATVEAVDVPVTLKMRLGWDERSLNAPELARRAEAAGVQLITVHGRTRQQFY
KGEADWSAVRAVKQAVDLPVVVNGDITSYEDAREALDASGADAVMIGRGAQGQPWLPGQIGRRLDGGAEEGAPELTQQLA
LLTALYDEICRHYGERVGLRHARKHLSWGLDVAAAHAQAPAEKLKAWRERILTSEQPSQVHRALREAFDDFAWSAAA

Sequences:

>Translated_317_residues
MSGITDAPFRRLAAELGAGLVVSEMTASDDLVRGRWISRLRCEAGGIGPHVVQLAGCEAHWMAEGAKVAADGGADIIDIN
MGCPARHVTGGQSGSALMRDLDHALTLIEATVEAVDVPVTLKMRLGWDERSLNAPELARRAEAAGVQLITVHGRTRQQFY
KGEADWSAVRAVKQAVDLPVVVNGDITSYEDAREALDASGADAVMIGRGAQGQPWLPGQIGRRLDGGAEEGAPELTQQLA
LLTALYDEICRHYGERVGLRHARKHLSWGLDVAAAHAQAPAEKLKAWRERILTSEQPSQVHRALREAFDDFAWSAAA
>Mature_316_residues
SGITDAPFRRLAAELGAGLVVSEMTASDDLVRGRWISRLRCEAGGIGPHVVQLAGCEAHWMAEGAKVAADGGADIIDINM
GCPARHVTGGQSGSALMRDLDHALTLIEATVEAVDVPVTLKMRLGWDERSLNAPELARRAEAAGVQLITVHGRTRQQFYK
GEADWSAVRAVKQAVDLPVVVNGDITSYEDAREALDASGADAVMIGRGAQGQPWLPGQIGRRLDGGAEEGAPELTQQLAL
LTALYDEICRHYGERVGLRHARKHLSWGLDVAAAHAQAPAEKLKAWRERILTSEQPSQVHRALREAFDDFAWSAAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=227, Percent_Identity=34.3612334801762, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI239788462, Length=227, Percent_Identity=34.3612334801762, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI40807366, Length=227, Percent_Identity=31.7180616740088, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI31742496, Length=211, Percent_Identity=28.9099526066351, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI8923374, Length=237, Percent_Identity=27.0042194092827, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI1789660, Length=313, Percent_Identity=44.408945686901, Blast_Score=237, Evalue=6e-64,
Organism=Escherichia coli, GI1788462, Length=231, Percent_Identity=35.0649350649351, Blast_Score=120, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17543114, Length=279, Percent_Identity=30.4659498207885, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI25144369, Length=216, Percent_Identity=31.0185185185185, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17507177, Length=299, Percent_Identity=27.4247491638796, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323560, Length=216, Percent_Identity=32.4074074074074, Blast_Score=93, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6323433, Length=247, Percent_Identity=31.1740890688259, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323437, Length=221, Percent_Identity=27.1493212669683, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24585320, Length=279, Percent_Identity=31.1827956989247, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24580595, Length=170, Percent_Identity=37.0588235294118, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI19920448, Length=170, Percent_Identity=37.0588235294118, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI45549423, Length=237, Percent_Identity=30.8016877637131, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19921524, Length=217, Percent_Identity=31.7972350230415, Blast_Score=92, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 34057; Mature: 33926

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGITDAPFRRLAAELGAGLVVSEMTASDDLVRGRWISRLRCEAGGIGPHVVQLAGCEAH
CCCCCHHHHHHHHHHHCCCEEEEHHCCCCHHHHHHHHHHHHHCCCCCCHHHEEEECCCHH
WMAEGAKVAADGGADIIDINMGCPARHVTGGQSGSALMRDLDHALTLIEATVEAVDVPVT
HHHCCCEEEECCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEE
LKMRLGWDERSLNAPELARRAEAAGVQLITVHGRTRQQFYKGEADWSAVRAVKQAVDLPV
EEEEECCCCCCCCHHHHHHHHHHCCEEEEEECCCHHHHHHCCCCHHHHHHHHHHHHCCCE
VVNGDITSYEDAREALDASGADAVMIGRGAQGQPWLPGQIGRRLDGGAEEGAPELTQQLA
EECCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHH
LLTALYDEICRHYGERVGLRHARKHLSWGLDVAAAHAQAPAEKLKAWRERILTSEQPSQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHCCCCCHHHH
HRALREAFDDFAWSAAA
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SGITDAPFRRLAAELGAGLVVSEMTASDDLVRGRWISRLRCEAGGIGPHVVQLAGCEAH
CCCCHHHHHHHHHHHCCCEEEEHHCCCCHHHHHHHHHHHHHCCCCCCHHHEEEECCCHH
WMAEGAKVAADGGADIIDINMGCPARHVTGGQSGSALMRDLDHALTLIEATVEAVDVPVT
HHHCCCEEEECCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEE
LKMRLGWDERSLNAPELARRAEAAGVQLITVHGRTRQQFYKGEADWSAVRAVKQAVDLPV
EEEEECCCCCCCCHHHHHHHHHHCCEEEEEECCCHHHHHHCCCCHHHHHHHHHHHHCCCE
VVNGDITSYEDAREALDASGADAVMIGRGAQGQPWLPGQIGRRLDGGAEEGAPELTQQLA
EECCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHH
LLTALYDEICRHYGERVGLRHARKHLSWGLDVAAAHAQAPAEKLKAWRERILTSEQPSQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHCCCCCHHHH
HRALREAFDDFAWSAAA
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8412703 [H]