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The map label for this gene is ntrC [H]

Identifier: 146340807

GI number: 146340807

Start: 4040947

End: 4042389

Strand: Reverse

Name: ntrC [H]

Synonym: BRADO3866

Alternate gene names: 146340807

Gene position: 4042389-4040947 (Counterclockwise)

Preceding gene: 146340808

Following gene: 146340806

Centisome position: 54.21

GC content: 67.91

Gene sequence:

>1443_bases
ATGCCTGCAGGTAGCATTCTCGTTGCCGACGACGATACGGCCATCCGCACGGTCCTGAACCAGGCGCTGTCACGCGCCGG
CTACGAGGTGCGGCTGACCGGAAACGCCGCCACACTGTGGCGATGGGTGAGCCAAGGGGAGGGCGATCTCGTCATCACCG
ACGTGGTGATGCCCGACGAGAACGCGTTCGACCTGCTGCCGCGGATCAAGAAGATGCGGCCGAACCTGCCGGTCATCGTG
ATGAGCGCGCAGAACACCTTCATGACGGCGATCAAGGCCTCCGAGCGCGGCGCCTATGAGTATCTGCCAAAACCGTTCGA
CCTGAAGGAGCTGATCGCGATCGTCGGCCGGGCGCTCGCCGAGCCGAAGGAGCGGGCGGCGAGCCAGCCCGACGACAGCG
AGTTCGAGTCGATCCCGCTGGTCGGCCGCTCGCCGGCGATGCAGGATATCTACCGCGTGCTGGCGCGGCTGATGCAGACC
GACCTCACGGTGATGATCTCCGGCGAGAGCGGCACCGGCAAGGAGCTGGTGGCGCGCGCGCTGCACGACTACGGCAAGCG
CCGCAACGGCCCGTTCGTCGCGGTCAACATGGCGGCGATCCCGCGCGACCTGATCGAATCGGAGCTGTTCGGCCATGAGC
GCGGCGCCTTCACTGGCGCCAACACCCGCGCCTCCGGCCGCTTCGAGCAGGCCGAGGGCGGCACGCTATTTTTGGACGAG
ATCGGCGACATGCCGATGGAGGCGCAGACGCGATTGCTCCGCGTGCTGCAGCAGGGCGAATACACCACCGTCGGCGGCCG
CACCCCGATCAAGACCGACGTCCGCATCGTCGCGGCCTCCAACAAGGACCTGCGCATCCTGATCCAGCAGGGCCTGTTCC
GCGAGGATCTGTTCTTCCGCCTCAACGTCGTGCCGCTGCGGCTGCCGCCGTTGCGCGAGCGCATCGAGGACCTGCCTGAC
CTCGTCCGGCACTTCTTCGCGCTGGCGGAGAAAGACGGGCTGCCGCCGAAGAAGCTGGATGCCGCGGCGCTGGAGCGGCT
CAAGCAGCACCGCTGGCCGGGCAACGTCCGCGAGCTCGAAAACCTCGCCCGGCGCCTCGCCGCGCTGTATCCCCAGGACG
TGATCACGGCCTCGGTGATCGATGGCGAGCTGGCGCCGCCGGCGGTCACCACCGGCTCGAACGCGCCGACCTCGATCGAC
AATCTCGGGGGCGCCGTCGAGGCCTATCTATCGTCGCACTTCCAGGGCTTTCCGAACGGCGTGCCGCCGCCGGGCCTCTA
TCACCGCATCCTGAAGGAGATCGAGATCCCGCTGCTCACGGCCGCGCTGGCCGCGACCCGCGGCAACCAGATCCGCGCCG
CCGATCTGCTCGGTCTCAACCGCAACACGCTGCGCAAGAAGATCCGCGACCTCGACATCCAGGTCTATCGCAGCGGCGGC
TAG

Upstream 100 bases:

>100_bases
GCCGATGTACAGCGCGAGCAAATATCCAGACCAAGGCCATCGCGATGACGTTTCGGGGACGCCGTCGCATGCCTCACAGG
GATCAAATTGAGGACCACCT

Downstream 100 bases:

>100_bases
ATCCGAGGAAAATCTCCCCCGATCATGTCAGGGGAGATTGCTGTGGCACGGGGCTGAGCGTCCGAACGCAGTCGCCCCCA
CCCGCCGTCCAGCGTTTCAC

Product: nitrogen assimilation regulatory protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MPAGSILVADDDTAIRTVLNQALSRAGYEVRLTGNAATLWRWVSQGEGDLVITDVVMPDENAFDLLPRIKKMRPNLPVIV
MSAQNTFMTAIKASERGAYEYLPKPFDLKELIAIVGRALAEPKERAASQPDDSEFESIPLVGRSPAMQDIYRVLARLMQT
DLTVMISGESGTGKELVARALHDYGKRRNGPFVAVNMAAIPRDLIESELFGHERGAFTGANTRASGRFEQAEGGTLFLDE
IGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRIVAASNKDLRILIQQGLFREDLFFRLNVVPLRLPPLRERIEDLPD
LVRHFFALAEKDGLPPKKLDAAALERLKQHRWPGNVRELENLARRLAALYPQDVITASVIDGELAPPAVTTGSNAPTSID
NLGGAVEAYLSSHFQGFPNGVPPPGLYHRILKEIEIPLLTAALAATRGNQIRAADLLGLNRNTLRKKIRDLDIQVYRSGG

Sequences:

>Translated_480_residues
MPAGSILVADDDTAIRTVLNQALSRAGYEVRLTGNAATLWRWVSQGEGDLVITDVVMPDENAFDLLPRIKKMRPNLPVIV
MSAQNTFMTAIKASERGAYEYLPKPFDLKELIAIVGRALAEPKERAASQPDDSEFESIPLVGRSPAMQDIYRVLARLMQT
DLTVMISGESGTGKELVARALHDYGKRRNGPFVAVNMAAIPRDLIESELFGHERGAFTGANTRASGRFEQAEGGTLFLDE
IGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRIVAASNKDLRILIQQGLFREDLFFRLNVVPLRLPPLRERIEDLPD
LVRHFFALAEKDGLPPKKLDAAALERLKQHRWPGNVRELENLARRLAALYPQDVITASVIDGELAPPAVTTGSNAPTSID
NLGGAVEAYLSSHFQGFPNGVPPPGLYHRILKEIEIPLLTAALAATRGNQIRAADLLGLNRNTLRKKIRDLDIQVYRSGG
>Mature_479_residues
PAGSILVADDDTAIRTVLNQALSRAGYEVRLTGNAATLWRWVSQGEGDLVITDVVMPDENAFDLLPRIKKMRPNLPVIVM
SAQNTFMTAIKASERGAYEYLPKPFDLKELIAIVGRALAEPKERAASQPDDSEFESIPLVGRSPAMQDIYRVLARLMQTD
LTVMISGESGTGKELVARALHDYGKRRNGPFVAVNMAAIPRDLIESELFGHERGAFTGANTRASGRFEQAEGGTLFLDEI
GDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRIVAASNKDLRILIQQGLFREDLFFRLNVVPLRLPPLRERIEDLPDL
VRHFFALAEKDGLPPKKLDAAALERLKQHRWPGNVRELENLARRLAALYPQDVITASVIDGELAPPAVTTGSNAPTSIDN
LGGAVEAYLSSHFQGFPNGVPPPGLYHRILKEIEIPLLTAALAATRGNQIRAADLLGLNRNTLRKKIRDLDIQVYRSGG

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=483, Percent_Identity=45.9627329192547, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1788550, Length=475, Percent_Identity=37.8947368421053, Blast_Score=317, Evalue=9e-88,
Organism=Escherichia coli, GI1790437, Length=466, Percent_Identity=39.6995708154506, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1788905, Length=464, Percent_Identity=36.6379310344828, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1789233, Length=355, Percent_Identity=41.4084507042254, Blast_Score=251, Evalue=6e-68,
Organism=Escherichia coli, GI1789087, Length=265, Percent_Identity=49.0566037735849, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI87082117, Length=231, Percent_Identity=50.6493506493506, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI87082152, Length=243, Percent_Identity=46.5020576131687, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1786524, Length=250, Percent_Identity=45.6, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI87081872, Length=342, Percent_Identity=38.3040935672515, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1787583, Length=252, Percent_Identity=41.2698412698413, Blast_Score=181, Evalue=9e-47,
Organism=Escherichia coli, GI1789828, Length=324, Percent_Identity=30.2469135802469, Blast_Score=132, Evalue=7e-32,
Organism=Escherichia coli, GI87081858, Length=204, Percent_Identity=36.7647058823529, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1789809, Length=109, Percent_Identity=32.1100917431193, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786784, Length=232, Percent_Identity=26.2931034482759, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1790552, Length=115, Percent_Identity=34.7826086956522, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52862; Mature: 52731

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAGSILVADDDTAIRTVLNQALSRAGYEVRLTGNAATLWRWVSQGEGDLVITDVVMPDE
CCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCEEEEEEECCCC
NAFDLLPRIKKMRPNLPVIVMSAQNTFMTAIKASERGAYEYLPKPFDLKELIAIVGRALA
CHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHC
EPKERAASQPDDSEFESIPLVGRSPAMQDIYRVLARLMQTDLTVMISGESGTGKELVARA
CHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHH
LHDYGKRRNGPFVAVNMAAIPRDLIESELFGHERGAFTGANTRASGRFEQAEGGTLFLDE
HHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCEEEHHH
IGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRIVAASNKDLRILIQQGLFREDLFFR
HCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEECCCCHHHHHHHCCHHHCCEEE
LNVVPLRLPPLRERIEDLPDLVRHFFALAEKDGLPPKKLDAAALERLKQHRWPGNVRELE
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
NLARRLAALYPQDVITASVIDGELAPPAVTTGSNAPTSIDNLGGAVEAYLSSHFQGFPNG
HHHHHHHHHCCHHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
VPPPGLYHRILKEIEIPLLTAALAATRGNQIRAADLLGLNRNTLRKKIRDLDIQVYRSGG
CCCCHHHHHHHHHHCHHHHHHHHHHHCCCCEEEHHHHCCCHHHHHHHHHCCCEEEEECCC
>Mature Secondary Structure 
PAGSILVADDDTAIRTVLNQALSRAGYEVRLTGNAATLWRWVSQGEGDLVITDVVMPDE
CCCCEEEECCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCEEEEEEECCCC
NAFDLLPRIKKMRPNLPVIVMSAQNTFMTAIKASERGAYEYLPKPFDLKELIAIVGRALA
CHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHC
EPKERAASQPDDSEFESIPLVGRSPAMQDIYRVLARLMQTDLTVMISGESGTGKELVARA
CHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHH
LHDYGKRRNGPFVAVNMAAIPRDLIESELFGHERGAFTGANTRASGRFEQAEGGTLFLDE
HHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCEEEHHH
IGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRIVAASNKDLRILIQQGLFREDLFFR
HCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEECCCCHHHHHHHCCHHHCCEEE
LNVVPLRLPPLRERIEDLPDLVRHFFALAEKDGLPPKKLDAAALERLKQHRWPGNVRELE
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
NLARRLAALYPQDVITASVIDGELAPPAVTTGSNAPTSIDNLGGAVEAYLSSHFQGFPNG
HHHHHHHHHCCHHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
VPPPGLYHRILKEIEIPLLTAALAATRGNQIRAADLLGLNRNTLRKKIRDLDIQVYRSGG
CCCCHHHHHHHHHHCHHHHHHHHHHHCCCCEEEHHHHCCCHHHHHHHHHCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3020561 [H]