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The map label for this gene is kipI [H]

Identifier: 146340743

GI number: 146340743

Start: 3969876

End: 3970607

Strand: Reverse

Name: kipI [H]

Synonym: BRADO3798

Alternate gene names: 146340743

Gene position: 3970607-3969876 (Counterclockwise)

Preceding gene: 146340746

Following gene: 146340742

Centisome position: 53.25

GC content: 69.4

Gene sequence:

>732_bases
ATGGCCGATCCTTTTCCGCCCCCTCGCCTGCTGCCCAGCGGCGACAGCGCCGTCACCGTAGAGTTCAGCCGGACCATCGA
CGAGACGGCGAACCGCCGTGTGCTCGCGCTCGATCATGCGCTGGCGGCGGCGGGCATCGCGGGACTTACCGAGACGGTGC
CGACCTATCGCTCGCTCCTGGTGCACTACGATCCGGGCTCGATCGATTACGATACGCTCTGCGCCAGGCTGCTGGCGCTG
TCGGCCGGGTCGCTGGCGCCGGAGACCGATATCAGGCGCTGGCGGGTGCCGGTCTGCTATGGCGGCGAGAACGGTATCGA
CCTCGAGGACGTCGCCAAGGCGCTCAGACTGACGCCCGACGAGGTCGTCGCGCGCCATGTCGCGGGCACCTATCGCGTGG
CGATGATCGGCTTCACGCCCGGCTGGTCCTATCTGTCGGGCCTCGATCCCTCACTGCAGATGTCACGCCGGCAGAACCCG
CGGCTGGCGACGCCGGCCGGCATCATCGCGATCGGCGGCATCCAGACCGGCATCCAGTGCCTGGCCGGCCCGTGCGGCTG
GCATCTGCTCGGCCGCACCGCCATGCGAACGTTCCAACTGCATCGCGAGCCGACCTTCCTGCTCGAGCCGGGCGACGAGG
TCAGCTTCACGGCGGTGGACGAGAAGATCTTCGCCGAGCAGGAGCGCGCGGCCGCAGCCGGCGAGCTCATCGCGGAGCGG
ATCGCGGCATGA

Upstream 100 bases:

>100_bases
TAATTTATTGAATTTTCGAGAGTTTTTGATTTTGACCCAATTCCACTTGCAGCCGAAAGCAGCTAATCAGGAACCAGTAC
CCGAAACGATTTCGCGACTC

Downstream 100 bases:

>100_bases
GCATGCTCGTCGTTGCGCAGATCGGTCCGGCAAGCTCGGTGCAGGATGGTGGCCGCTTCGGCGCGCAGCGCTACGGGCTG
ACGCCATCGGGCGCGATGGA

Product: putative carboxylase

Products: NA

Alternate protein names: Sporulation inhibitor kipI [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MADPFPPPRLLPSGDSAVTVEFSRTIDETANRRVLALDHALAAAGIAGLTETVPTYRSLLVHYDPGSIDYDTLCARLLAL
SAGSLAPETDIRRWRVPVCYGGENGIDLEDVAKALRLTPDEVVARHVAGTYRVAMIGFTPGWSYLSGLDPSLQMSRRQNP
RLATPAGIIAIGGIQTGIQCLAGPCGWHLLGRTAMRTFQLHREPTFLLEPGDEVSFTAVDEKIFAEQERAAAAGELIAER
IAA

Sequences:

>Translated_243_residues
MADPFPPPRLLPSGDSAVTVEFSRTIDETANRRVLALDHALAAAGIAGLTETVPTYRSLLVHYDPGSIDYDTLCARLLAL
SAGSLAPETDIRRWRVPVCYGGENGIDLEDVAKALRLTPDEVVARHVAGTYRVAMIGFTPGWSYLSGLDPSLQMSRRQNP
RLATPAGIIAIGGIQTGIQCLAGPCGWHLLGRTAMRTFQLHREPTFLLEPGDEVSFTAVDEKIFAEQERAAAAGELIAER
IAA
>Mature_242_residues
ADPFPPPRLLPSGDSAVTVEFSRTIDETANRRVLALDHALAAAGIAGLTETVPTYRSLLVHYDPGSIDYDTLCARLLALS
AGSLAPETDIRRWRVPVCYGGENGIDLEDVAKALRLTPDEVVARHVAGTYRVAMIGFTPGWSYLSGLDPSLQMSRRQNPR
LATPAGIIAIGGIQTGIQCLAGPCGWHLLGRTAMRTFQLHREPTFLLEPGDEVSFTAVDEKIFAEQERAAAAGELIAERI
AA

Specific function: Is a potent inhibitor of the autophosphorylation reaction of kinase A and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786929, Length=211, Percent_Identity=35.0710900473934, Blast_Score=117, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: NA

Molecular weight: Translated: 26163; Mature: 26032

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADPFPPPRLLPSGDSAVTVEFSRTIDETANRRVLALDHALAAAGIAGLTETVPTYRSLL
CCCCCCCCCCCCCCCCEEEEEEHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHEEE
VHYDPGSIDYDTLCARLLALSAGSLAPETDIRRWRVPVCYGGENGIDLEDVAKALRLTPD
EEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHEECEEEECCCCCCCHHHHHHHHCCCHH
EVVARHVAGTYRVAMIGFTPGWSYLSGLDPSLQMSRRQNPRLATPAGIIAIGGIQTGIQC
HHHHHHHCCCEEEEEEEECCCHHHHHCCCHHHHHHHCCCCCCCCCCCEEEECCHHHHHHH
LAGPCGWHLLGRTAMRTFQLHREPTFLLEPGDEVSFTAVDEKIFAEQERAAAAGELIAER
HCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
IAA
HCC
>Mature Secondary Structure 
ADPFPPPRLLPSGDSAVTVEFSRTIDETANRRVLALDHALAAAGIAGLTETVPTYRSLL
CCCCCCCCCCCCCCCEEEEEEHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHEEE
VHYDPGSIDYDTLCARLLALSAGSLAPETDIRRWRVPVCYGGENGIDLEDVAKALRLTPD
EEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHEECEEEECCCCCCCHHHHHHHHCCCHH
EVVARHVAGTYRVAMIGFTPGWSYLSGLDPSLQMSRRQNPRLATPAGIIAIGGIQTGIQC
HHHHHHHCCCEEEEEEEECCCHHHHHCCCHHHHHHHCCCCCCCCCCCEEEECCHHHHHHH
LAGPCGWHLLGRTAMRTFQLHREPTFLLEPGDEVSFTAVDEKIFAEQERAAAAGELIAER
HCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
IAA
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]