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The map label for this gene is paaF [C]

Identifier: 146340659

GI number: 146340659

Start: 3870700

End: 3871467

Strand: Reverse

Name: paaF [C]

Synonym: BRADO3707

Alternate gene names: 146340659

Gene position: 3871467-3870700 (Counterclockwise)

Preceding gene: 146340662

Following gene: 146340658

Centisome position: 51.92

GC content: 68.49

Gene sequence:

>768_bases
ATGACCCCGTCCTATTTCACCCGCTTCGCCCATCTGCGGCTGACCCGCGATGCCCAGGGCGTGCTCCTGGTCGAGATGCA
CAGCGACGGCGGGCCGATCCGCTTCGATGCGCGCGACCACACCGAATTTACCGACGCCTTCTATGAGATCGGCCGCGACC
GCGACAACAAGATCGTGATCCTGACGGGCGTCGGCGACTACATGGCCGAGATCGACTTCGCGAGCTTCGGCAATGTCGGC
GATGCCAACGCCTGGTCGCGGGTCCATGACGAGGGCACGCAGATCCTGGAGAACATCGCCAACATCCGCGTGCCGCTGAT
CGCCGCGATCGAGGGCCGCGCCCACATCCACACCGAATACGCGCTGTTCGCCGACGTCGTCATTGCCGGCCGCGGCGCCA
CCTTCCACGATCTGCCGCACTTCGCCGGCGGCATCGTGCCGGGCGACGGCATCTTCACGACCTGGTCGTATCGCGCCGGC
GCCGGCCGCGCCCAGGCCTTCCTCCTGAACCCGCAGCCGCTGTCGGCGCAGGAGGCCGCGAACTGGGGCGTCGTCAACCA
CGTCGTCGACGACGGCACCACGGTCGCCTTCGCCCGCGAGCTCGCCGCGCTCTATCTGCGCCAGCCCGAGGTCACCCGCC
GCAACAGCCGCATCCATTTCGTCCAGCCGCTGAAGGAGCGCATCGTCCGCGAGGTCGGCTATGGCCTCGCCCTCGAGGGC
GCCAGCGCCAACGCCCTGGTGCAGCAGATGATGGCGGGACAGCGCTGA

Upstream 100 bases:

>100_bases
GATATCGATCCGACGAGAATTATCCAAGGGCACCGAATGAGCCATGCTCCTCCCATCAGCAACGGCCGGCGCATCGCCGG
CAACCGCCACGGAGAGAGCC

Downstream 100 bases:

>100_bases
GTTCGTCCGCTCGATGTGAACCGAGCTCATCCTCAACAACGCTGCGCTCCCTCTCCCCGTTCTTGGGCGCACGTGAGGAG
CTTCGCTCGCGCTGAGAGGG

Product: putative 6-oxocamphor hydrolase

Products: trans(or 3)-enoyl-CoA; H2O [C]

Alternate protein names: Enoyl-CoA Hydratase; Enoyl-CoA Hydratase EchA; 6-Oxocamphor Hydrolase; Short Chain Enoyl-CoA Hydratase; Enoyl-CoA Hydratase/Carnithine Racemase; Enoyl-CoA Hydratase/Isomerase Family; Enoyl-CoA Hydratase/Isomerase Family Protein

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MTPSYFTRFAHLRLTRDAQGVLLVEMHSDGGPIRFDARDHTEFTDAFYEIGRDRDNKIVILTGVGDYMAEIDFASFGNVG
DANAWSRVHDEGTQILENIANIRVPLIAAIEGRAHIHTEYALFADVVIAGRGATFHDLPHFAGGIVPGDGIFTTWSYRAG
AGRAQAFLLNPQPLSAQEAANWGVVNHVVDDGTTVAFARELAALYLRQPEVTRRNSRIHFVQPLKERIVREVGYGLALEG
ASANALVQQMMAGQR

Sequences:

>Translated_255_residues
MTPSYFTRFAHLRLTRDAQGVLLVEMHSDGGPIRFDARDHTEFTDAFYEIGRDRDNKIVILTGVGDYMAEIDFASFGNVG
DANAWSRVHDEGTQILENIANIRVPLIAAIEGRAHIHTEYALFADVVIAGRGATFHDLPHFAGGIVPGDGIFTTWSYRAG
AGRAQAFLLNPQPLSAQEAANWGVVNHVVDDGTTVAFARELAALYLRQPEVTRRNSRIHFVQPLKERIVREVGYGLALEG
ASANALVQQMMAGQR
>Mature_254_residues
TPSYFTRFAHLRLTRDAQGVLLVEMHSDGGPIRFDARDHTEFTDAFYEIGRDRDNKIVILTGVGDYMAEIDFASFGNVGD
ANAWSRVHDEGTQILENIANIRVPLIAAIEGRAHIHTEYALFADVVIAGRGATFHDLPHFAGGIVPGDGIFTTWSYRAGA
GRAQAFLLNPQPLSAQEAANWGVVNHVVDDGTTVAFARELAALYLRQPEVTRRNSRIHFVQPLKERIVREVGYGLALEGA
SANALVQQMMAGQR

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.2.1.17 [C]

Molecular weight: Translated: 28059; Mature: 27928

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPSYFTRFAHLRLTRDAQGVLLVEMHSDGGPIRFDARDHTEFTDAFYEIGRDRDNKIVI
CCCHHHHHHHHEEEECCCCCEEEEEEECCCCCEEECCCCCCHHHHHHHHHCCCCCCEEEE
LTGVGDYMAEIDFASFGNVGDANAWSRVHDEGTQILENIANIRVPLIAAIEGRAHIHTEY
EEECCHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCEEEEHHH
ALFADVVIAGRGATFHDLPHFAGGIVPGDGIFTTWSYRAGAGRAQAFLLNPQPLSAQEAA
HHHHHHHEECCCCCHHCCHHHHCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCHHHHC
NWGVVNHVVDDGTTVAFARELAALYLRQPEVTRRNSRIHFVQPLKERIVREVGYGLALEG
CCCHHEEECCCCCHHHHHHHHHHHHHCCCCHHHCCCEEEEHHHHHHHHHHHHCCCEEECC
ASANALVQQMMAGQR
CCHHHHHHHHHCCCC
>Mature Secondary Structure 
TPSYFTRFAHLRLTRDAQGVLLVEMHSDGGPIRFDARDHTEFTDAFYEIGRDRDNKIVI
CCHHHHHHHHEEEECCCCCEEEEEEECCCCCEEECCCCCCHHHHHHHHHCCCCCCEEEE
LTGVGDYMAEIDFASFGNVGDANAWSRVHDEGTQILENIANIRVPLIAAIEGRAHIHTEY
EEECCHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCEEEEHHH
ALFADVVIAGRGATFHDLPHFAGGIVPGDGIFTTWSYRAGAGRAQAFLLNPQPLSAQEAA
HHHHHHHEECCCCCHHCCHHHHCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCHHHHC
NWGVVNHVVDDGTTVAFARELAALYLRQPEVTRRNSRIHFVQPLKERIVREVGYGLALEG
CCCHHEEECCCCCHHHHHHHHHHHHHCCCCHHHCCCEEEEHHHHHHHHHHHHCCCEEECC
ASANALVQQMMAGQR
CCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-hydroxyacyl-CoA [C]

Specific reaction: (3S)-hydroxyacyl-CoA = trans(or 3)-enoyl-CoA + H2O [C]

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA