Click here to switch to the map view.

The map label for this gene is pbpG [H]

Identifier: 146337656

GI number: 146337656

Start: 530680

End: 532914

Strand: Reverse

Name: pbpG [H]

Synonym: BRADO0522

Alternate gene names: 146337656

Gene position: 532914-530680 (Counterclockwise)

Preceding gene: 146337661

Following gene: 146337650

Centisome position: 7.15

GC content: 67.96

Gene sequence:

>2235_bases
TTGCGTATGGCGCGGGGACGGAAACAGGGCGGCGGCGGACGCAAGGAGCCGCGATTCGGCCTTGCGGCGGCGATGGCCGA
TCTGCGCCTCGATCCCAAGGACCGCATTCCGGCCGGCGAGGACGCGCCGAAGAAATCAACCAACAAGAAGAAGCCGGTCG
AGCCGGACGACGAGGACGATGTGCCGGCGCGCGAGCCCAAGGCCAACCAGCCGAAACCCGCGCCAGCGAAATCGAAGCCC
GCCCGCGCCAAGCCGCGATGGGGGACGTCGATCGGCCGCATGGCCTATTGGGCTGTCGTCTGCGGCCTGTGGGGCGTGAT
CGCGCTGGTCGGCGTCGTGATCTGGGCCGGCGCGCATCTGCCGCCGATCCAGTCGCTCGAAATCCCCAAGCGCCCGCCGA
CGATCGAGATCGTGGGCGTCGATGGCACCATGCTGGCACAGCGCGGCGAGATGGCCGGCGCCAATGTCGCGCTGAAGGAT
TTGCCGCCCTACCTGCCCAAGGCGTTCATCGCCATCGAGGACCGCCGCTTCTACCAGCATTTCGGCATCGATCCGATCGG
CATCGCGCGGGCATTGGTCGCCAACATCCTGCACCGTGGCGTGTCGCAGGGTGGCTCAACGCTGACCCAGCAGCTCGCCA
AGAACCTGTTCCTGACGCAGGAGCGCACGCTCGCGCGCAAGCTGCAGGAGGCGGAACTGGCGCTGTGGCTGGAGCGCAAA
TATTCCAAGCGCGAGATCCTGGAGCTCTATCTGAACCGCGTCTATTTCGGTTCCGGCGCCTATGGCGTCGAGGCGGCGGC
GCAGCGTTATTTCGGCAAGTCGGCGCGGCAGGTGACGGTGGCCGAGGCTGCGATGCTCGCCGGCCTCGTCAAGTCGCCGT
CGCGGCTGGCGCCCAATCGGAATCCGGAAGGCGCGGCCAAGCGCGCGCAGACCGTGCTCGCGGCGATGGCCGACGCCAAG
TTCATCACCGAGGCACAGGCACAGGCACAGATCGCGCATCCCTCCGTCGCGGTGAAGCCTGCCGGCGCCGGCACCGTCAA
CTACGTCGCCGACTGGATCGGCGAGGTGCTCGACGATCTCGTCGGCCAGGTCGAGGACTCGATTGTCGTGGAGACCACGA
TCGATCCGAAGCTGCAGAGCGTGGCCGAAGCCGCTGTCATCGACGAGCTCGCGGCCAAGAGCGTGAAATTCAATGTCAGC
CAGGGCGCGCTGGTGGCGATGACGCCGGATGGTGCCGTGCGCGCCATGGTCGGCGGCCGCAACTATGCCGACAGCCAGTT
CAATCGCGCGGTCACCGCCAGGCGTCAGCCCGGCTCGGCATTCAAGCCGTTCGTCTATCTCACCGCGATCGAGCAGGGCC
TGACGCCCGATACGGTGCGCCAGGACGCGCCGCTGGATCTGAAGGGCTGGCGGCCGGAGAACTACACCAAGGAGTATTTC
GGGCCGGTGACGCTGACCCAGGCGCTGGCGATGTCGCTGAACACGGTCGCGGTCCGCATCGGCCTCGAGGTCGGGCCGAA
GAACGTCGTGCGCACCGCGCACCGTCTCGGCATCGCCTCCAAGCTCGATCCCAACCCGTCGATCGCGCTCGGCACCTCCG
AGGTGTCGATGATCGAGCTGGTCGGCGCCTATGCACCATTTTCCAATGGCGGCCTCGCCATATCGCCGCATGTGGTGACG
CGGATCAAGACGCTCGACGGCAAGCTGCTCTACATGCGTCAGACCGAGCAGCCGGCGCAGGTGATCGATCCGCGCAGCGT
CGCGATGATGAACCAGATGATGCAGGAGACGCTGATCTCCGGCACCGCCAAGAAGGCGGAGATCCCCGGCTGGGTTGCCG
CCGGCAAGACCGGCACCAGCCAGGATTTCCGCGACGCCTGGTTCATCGGCTACACCTCTCACCTGGTCACCGGCGTCTGG
CTCGGCAATGACGACAATTCGCCGACCAAGAAGGCCACCGGCGGCGGCCTGCCGGTCGAGGTGTGGACCCGCTTCATGCG
CGCGGCGCATCAGGGCGTGCCGGTCGCGGCGCTGCCGAATGCGCAAGGAGCAGGGCTCGTGCCGACGCTGGCGCAGATCG
CCTCGCAGATCACGGCGCCGACGGCCCCTGCTCCCACTGCCGCCTACTCCGCGCGAACGGCGGCGCCGGGCCCGAGCCGC
GGCAACGCGCGGCCCGATCCGGCGGCCGGACTCGACGGCTGGCTGGTGGATCGATTGTTCGGCGGCCAGCGCTGA

Upstream 100 bases:

>100_bases
ACGCGGCGTTCACACCGCGAAACTCCTAATCGCGCGTTAAGCTTTTCCTAACTGCCCCTTAAACTCCACTCATTACCGTG
GTTCGACGGCAGGGCTCGGG

Downstream 100 bases:

>100_bases
GCCGGATCGGCGACGCGTTCGCATCCACCACCCCCGTCAGTCCGGGTTCAATCGCCAACGCGCTGACGCGCGTCGCGCGT
CCTTGGGGAAAACGAGGCCC

Product: putative penicillin-binding protein pbpC/mrcB-like

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 744; Mature: 744

Protein sequence:

>744_residues
MRMARGRKQGGGGRKEPRFGLAAAMADLRLDPKDRIPAGEDAPKKSTNKKKPVEPDDEDDVPAREPKANQPKPAPAKSKP
ARAKPRWGTSIGRMAYWAVVCGLWGVIALVGVVIWAGAHLPPIQSLEIPKRPPTIEIVGVDGTMLAQRGEMAGANVALKD
LPPYLPKAFIAIEDRRFYQHFGIDPIGIARALVANILHRGVSQGGSTLTQQLAKNLFLTQERTLARKLQEAELALWLERK
YSKREILELYLNRVYFGSGAYGVEAAAQRYFGKSARQVTVAEAAMLAGLVKSPSRLAPNRNPEGAAKRAQTVLAAMADAK
FITEAQAQAQIAHPSVAVKPAGAGTVNYVADWIGEVLDDLVGQVEDSIVVETTIDPKLQSVAEAAVIDELAAKSVKFNVS
QGALVAMTPDGAVRAMVGGRNYADSQFNRAVTARRQPGSAFKPFVYLTAIEQGLTPDTVRQDAPLDLKGWRPENYTKEYF
GPVTLTQALAMSLNTVAVRIGLEVGPKNVVRTAHRLGIASKLDPNPSIALGTSEVSMIELVGAYAPFSNGGLAISPHVVT
RIKTLDGKLLYMRQTEQPAQVIDPRSVAMMNQMMQETLISGTAKKAEIPGWVAAGKTGTSQDFRDAWFIGYTSHLVTGVW
LGNDDNSPTKKATGGGLPVEVWTRFMRAAHQGVPVAALPNAQGAGLVPTLAQIASQITAPTAPAPTAAYSARTAAPGPSR
GNARPDPAAGLDGWLVDRLFGGQR

Sequences:

>Translated_744_residues
MRMARGRKQGGGGRKEPRFGLAAAMADLRLDPKDRIPAGEDAPKKSTNKKKPVEPDDEDDVPAREPKANQPKPAPAKSKP
ARAKPRWGTSIGRMAYWAVVCGLWGVIALVGVVIWAGAHLPPIQSLEIPKRPPTIEIVGVDGTMLAQRGEMAGANVALKD
LPPYLPKAFIAIEDRRFYQHFGIDPIGIARALVANILHRGVSQGGSTLTQQLAKNLFLTQERTLARKLQEAELALWLERK
YSKREILELYLNRVYFGSGAYGVEAAAQRYFGKSARQVTVAEAAMLAGLVKSPSRLAPNRNPEGAAKRAQTVLAAMADAK
FITEAQAQAQIAHPSVAVKPAGAGTVNYVADWIGEVLDDLVGQVEDSIVVETTIDPKLQSVAEAAVIDELAAKSVKFNVS
QGALVAMTPDGAVRAMVGGRNYADSQFNRAVTARRQPGSAFKPFVYLTAIEQGLTPDTVRQDAPLDLKGWRPENYTKEYF
GPVTLTQALAMSLNTVAVRIGLEVGPKNVVRTAHRLGIASKLDPNPSIALGTSEVSMIELVGAYAPFSNGGLAISPHVVT
RIKTLDGKLLYMRQTEQPAQVIDPRSVAMMNQMMQETLISGTAKKAEIPGWVAAGKTGTSQDFRDAWFIGYTSHLVTGVW
LGNDDNSPTKKATGGGLPVEVWTRFMRAAHQGVPVAALPNAQGAGLVPTLAQIASQITAPTAPAPTAAYSARTAAPGPSR
GNARPDPAAGLDGWLVDRLFGGQR
>Mature_744_residues
MRMARGRKQGGGGRKEPRFGLAAAMADLRLDPKDRIPAGEDAPKKSTNKKKPVEPDDEDDVPAREPKANQPKPAPAKSKP
ARAKPRWGTSIGRMAYWAVVCGLWGVIALVGVVIWAGAHLPPIQSLEIPKRPPTIEIVGVDGTMLAQRGEMAGANVALKD
LPPYLPKAFIAIEDRRFYQHFGIDPIGIARALVANILHRGVSQGGSTLTQQLAKNLFLTQERTLARKLQEAELALWLERK
YSKREILELYLNRVYFGSGAYGVEAAAQRYFGKSARQVTVAEAAMLAGLVKSPSRLAPNRNPEGAAKRAQTVLAAMADAK
FITEAQAQAQIAHPSVAVKPAGAGTVNYVADWIGEVLDDLVGQVEDSIVVETTIDPKLQSVAEAAVIDELAAKSVKFNVS
QGALVAMTPDGAVRAMVGGRNYADSQFNRAVTARRQPGSAFKPFVYLTAIEQGLTPDTVRQDAPLDLKGWRPENYTKEYF
GPVTLTQALAMSLNTVAVRIGLEVGPKNVVRTAHRLGIASKLDPNPSIALGTSEVSMIELVGAYAPFSNGGLAISPHVVT
RIKTLDGKLLYMRQTEQPAQVIDPRSVAMMNQMMQETLISGTAKKAEIPGWVAAGKTGTSQDFRDAWFIGYTSHLVTGVW
LGNDDNSPTKKATGGGLPVEVWTRFMRAAHQGVPVAALPNAQGAGLVPTLAQIASQITAPTAPAPTAAYSARTAAPGPSR
GNARPDPAAGLDGWLVDRLFGGQR

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=547, Percent_Identity=33.6380255941499, Blast_Score=238, Evalue=1e-63,
Organism=Escherichia coli, GI1788867, Length=566, Percent_Identity=31.9787985865724, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI87082258, Length=294, Percent_Identity=40.4761904761905, Blast_Score=204, Evalue=2e-53,
Organism=Escherichia coli, GI1789601, Length=155, Percent_Identity=43.2258064516129, Blast_Score=118, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 79535; Mature: 79535

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMARGRKQGGGGRKEPRFGLAAAMADLRLDPKDRIPAGEDAPKKSTNKKKPVEPDDEDD
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCC
VPAREPKANQPKPAPAKSKPARAKPRWGTSIGRMAYWAVVCGLWGVIALVGVVIWAGAHL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PPIQSLEIPKRPPTIEIVGVDGTMLAQRGEMAGANVALKDLPPYLPKAFIAIEDRRFYQH
CCCCCCCCCCCCCEEEEEECCCHHHHHCCCCCCCCEEHHHCCCCCCCEEEEEEHHHHHHH
FGIDPIGIARALVANILHRGVSQGGSTLTQQLAKNLFLTQERTLARKLQEAELALWLERK
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSKREILELYLNRVYFGSGAYGVEAAAQRYFGKSARQVTVAEAAMLAGLVKSPSRLAPNR
CCHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCCCHHCCCCC
NPEGAAKRAQTVLAAMADAKFITEAQAQAQIAHPSVAVKPAGAGTVNYVADWIGEVLDDL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHH
VGQVEDSIVVETTIDPKLQSVAEAAVIDELAAKSVKFNVSQGALVAMTPDGAVRAMVGGR
HHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEECCCCCEEEEECCC
NYADSQFNRAVTARRQPGSAFKPFVYLTAIEQGLTPDTVRQDAPLDLKGWRPENYTKEYF
CCCHHHHHHHHHHCCCCCCCCCCEEEEEHHHHCCCHHHHCCCCCCCCCCCCCCCCCHHHC
GPVTLTQALAMSLNTVAVRIGLEVGPKNVVRTAHRLGIASKLDPNPSIALGTSEVSMIEL
CHHHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHH
VGAYAPFSNGGLAISPHVVTRIKTLDGKLLYMRQTEQPAQVIDPRSVAMMNQMMQETLIS
HHHHCCCCCCCEEECCHHHHHEECCCCEEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHC
GTAKKAEIPGWVAAGKTGTSQDFRDAWFIGYTSHLVTGVWLGNDDNSPTKKATGGGLPVE
CCCCCCCCCCEEECCCCCCCCCHHHHEEEEEHHHHHEEEEECCCCCCCCCCCCCCCCCHH
VWTRFMRAAHQGVPVAALPNAQGAGLVPTLAQIASQITAPTAPAPTAAYSARTAAPGPSR
HHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCC
GNARPDPAAGLDGWLVDRLFGGQR
CCCCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MRMARGRKQGGGGRKEPRFGLAAAMADLRLDPKDRIPAGEDAPKKSTNKKKPVEPDDEDD
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCC
VPAREPKANQPKPAPAKSKPARAKPRWGTSIGRMAYWAVVCGLWGVIALVGVVIWAGAHL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PPIQSLEIPKRPPTIEIVGVDGTMLAQRGEMAGANVALKDLPPYLPKAFIAIEDRRFYQH
CCCCCCCCCCCCCEEEEEECCCHHHHHCCCCCCCCEEHHHCCCCCCCEEEEEEHHHHHHH
FGIDPIGIARALVANILHRGVSQGGSTLTQQLAKNLFLTQERTLARKLQEAELALWLERK
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSKREILELYLNRVYFGSGAYGVEAAAQRYFGKSARQVTVAEAAMLAGLVKSPSRLAPNR
CCHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCCCHHCCCCC
NPEGAAKRAQTVLAAMADAKFITEAQAQAQIAHPSVAVKPAGAGTVNYVADWIGEVLDDL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHH
VGQVEDSIVVETTIDPKLQSVAEAAVIDELAAKSVKFNVSQGALVAMTPDGAVRAMVGGR
HHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEECCCCCEEEEECCC
NYADSQFNRAVTARRQPGSAFKPFVYLTAIEQGLTPDTVRQDAPLDLKGWRPENYTKEYF
CCCHHHHHHHHHHCCCCCCCCCCEEEEEHHHHCCCHHHHCCCCCCCCCCCCCCCCCHHHC
GPVTLTQALAMSLNTVAVRIGLEVGPKNVVRTAHRLGIASKLDPNPSIALGTSEVSMIEL
CHHHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHH
VGAYAPFSNGGLAISPHVVTRIKTLDGKLLYMRQTEQPAQVIDPRSVAMMNQMMQETLIS
HHHHCCCCCCCEEECCHHHHHEECCCCEEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHC
GTAKKAEIPGWVAAGKTGTSQDFRDAWFIGYTSHLVTGVWLGNDDNSPTKKATGGGLPVE
CCCCCCCCCCEEECCCCCCCCCHHHHEEEEEHHHHHEEEEECCCCCCCCCCCCCCCCCHH
VWTRFMRAAHQGVPVAALPNAQGAGLVPTLAQIASQITAPTAPAPTAAYSARTAAPGPSR
HHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCC
GNARPDPAAGLDGWLVDRLFGGQR
CCCCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]