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The map label for this gene is livK [C]

Identifier: 146337661

GI number: 146337661

Start: 535327

End: 536577

Strand: Reverse

Name: livK [C]

Synonym: BRADO0527

Alternate gene names: 146337661

Gene position: 536577-535327 (Counterclockwise)

Preceding gene: 146337662

Following gene: 146337656

Centisome position: 7.2

GC content: 61.31

Gene sequence:

>1251_bases
ATGAGAACGTCACTGTTCGGTCTCGTCGGCTTCAAGGCGTGGGCAATCGCGCTCGCTGCGTCGCTGACCGTCGCGCTGCC
TGCAACATCCGCCTACGCCCAGAAGAAATACGACACCGGCGCCACCGATACCGAGATCAAGATCGGTCAGACCGTGCCGT
TCTCGGGCCCGGCTTCCGCCTATGCCGGCATCGGCAAAACGCAAGCTGCGTATTTGCGCATGATCAACGAGAAGGGCGGC
ATCAATGGCCGCAAGCTCAATCTGATCCAGTATGACGATGCCTACTCGCCGCCCAAGGCGGTGGAGCAGGTGCGCAAGCT
GGTCGAAGGCGACGAGGTGCTGTTCACCTTCCAGATCATCGGCACGCCCTCGAACGCCGCCGTGCAGAAATATCTCAACG
CCAAGAAGGTGCCGCAGCTCTTGGCCTCGACCGGCGCCTCGCGCTTCACCGATCCGCAGAATTTCCCGTGGACCATCGCC
TACAATCCGAACTACCAGTCGGAGGGGCGGATCTACGCCAAATACATCCTCGCCAACCACCCCAACGCCAAGATCGGCAT
CCTCTACCAGAACGATGATCTCGGTCGCGACTACATCGCCGGCCTGCGCAGCGGCCTCGGCGCAAAAGCCGATTCGATGA
TCGTCTCGGAGGCGTCCTACGAGCTGTCCGACCCGACCGTGGACTCGCAGATCGTCAAGCTGAAATCCTCCGGCGCCGAC
CTGCTCTACAACGCCTCGACGCCGAAATTCGCGGCGCAGGCGATCAAGAAGCTCGCCGATCTCGGCTGGAAACCGGTGCA
TATCCTCGACATCAATGCGAGCCCGGTGTCGGCGACCCTGAAACCGGCCGGCCTCGACATCTCCAAGGACATCATCAGCA
CCAATTACGGCAAGGACCCGGCCGATCCGCAGTGGAAGGACGATCCGGGCGTGAAGGCCTATTTCGAATTCATGGACAAG
TATTACCCTGAAGGAGACAAGCTCAACACCGTCAACACCTACGGCTACTCGACCGCGGAACTGCTGGTGCAGATCCTGCG
CCAATGCGGCGACGATTTGACGCGGGAAAACGTGATGCGGCAAGCGGCCAACATCAAGGGCTTCGTCCCGAGCCTCGCGC
TGCCCGGTCTGTCGATCAACACCTCCCCGACCGACTTCCGCATCAACAAGCAGATGCAGATGATGCGCTTCAACGGCGAG
CGCTGGGAACTGTTCGGCCCGATCATCGAGGACACGGGGGCGGCGGGGTAG

Upstream 100 bases:

>100_bases
CGTTGCCTTGTCGACTTGCTTCCTCCACACTCGCGTGCAGCGATGGCGACAAAAGAACCAAGAAGAACCATCGACAGACA
TCACCGAAGGGAGGACTTGC

Downstream 100 bases:

>100_bases
GCCACTCTGCTTCCGCATCCACACTGTCATGCCCCGCGCAGGCGGGGCATCCAGTACGCCGCGGCCTCTCGGGTGATCAC
AATCGCCGCGGAGTACTGGG

Product: putative branched-chain amino acid ABC transporter periplasmic binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: None

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MRTSLFGLVGFKAWAIALAASLTVALPATSAYAQKKYDTGATDTEIKIGQTVPFSGPASAYAGIGKTQAAYLRMINEKGG
INGRKLNLIQYDDAYSPPKAVEQVRKLVEGDEVLFTFQIIGTPSNAAVQKYLNAKKVPQLLASTGASRFTDPQNFPWTIA
YNPNYQSEGRIYAKYILANHPNAKIGILYQNDDLGRDYIAGLRSGLGAKADSMIVSEASYELSDPTVDSQIVKLKSSGAD
LLYNASTPKFAAQAIKKLADLGWKPVHILDINASPVSATLKPAGLDISKDIISTNYGKDPADPQWKDDPGVKAYFEFMDK
YYPEGDKLNTVNTYGYSTAELLVQILRQCGDDLTRENVMRQAANIKGFVPSLALPGLSINTSPTDFRINKQMQMMRFNGE
RWELFGPIIEDTGAAG

Sequences:

>Translated_416_residues
MRTSLFGLVGFKAWAIALAASLTVALPATSAYAQKKYDTGATDTEIKIGQTVPFSGPASAYAGIGKTQAAYLRMINEKGG
INGRKLNLIQYDDAYSPPKAVEQVRKLVEGDEVLFTFQIIGTPSNAAVQKYLNAKKVPQLLASTGASRFTDPQNFPWTIA
YNPNYQSEGRIYAKYILANHPNAKIGILYQNDDLGRDYIAGLRSGLGAKADSMIVSEASYELSDPTVDSQIVKLKSSGAD
LLYNASTPKFAAQAIKKLADLGWKPVHILDINASPVSATLKPAGLDISKDIISTNYGKDPADPQWKDDPGVKAYFEFMDK
YYPEGDKLNTVNTYGYSTAELLVQILRQCGDDLTRENVMRQAANIKGFVPSLALPGLSINTSPTDFRINKQMQMMRFNGE
RWELFGPIIEDTGAAG
>Mature_416_residues
MRTSLFGLVGFKAWAIALAASLTVALPATSAYAQKKYDTGATDTEIKIGQTVPFSGPASAYAGIGKTQAAYLRMINEKGG
INGRKLNLIQYDDAYSPPKAVEQVRKLVEGDEVLFTFQIIGTPSNAAVQKYLNAKKVPQLLASTGASRFTDPQNFPWTIA
YNPNYQSEGRIYAKYILANHPNAKIGILYQNDDLGRDYIAGLRSGLGAKADSMIVSEASYELSDPTVDSQIVKLKSSGAD
LLYNASTPKFAAQAIKKLADLGWKPVHILDINASPVSATLKPAGLDISKDIISTNYGKDPADPQWKDDPGVKAYFEFMDK
YYPEGDKLNTVNTYGYSTAELLVQILRQCGDDLTRENVMRQAANIKGFVPSLALPGLSINTSPTDFRINKQMQMMRFNGE
RWELFGPIIEDTGAAG

Specific function: This Protein Is A Component Of The Leucine-Specific Transport System, Which Is One Of The Two Periplasmic Binding Protein-Dependent Transport Systems Of The High-Affinity Transport Of The Branched-Chain Amino Acids In E.Coli. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 45341; Mature: 45341

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSLFGLVGFKAWAIALAASLTVALPATSAYAQKKYDTGATDTEIKIGQTVPFSGPASA
CCCCHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHCCCCCCCCEEEECCCCCCCCCHHH
YAGIGKTQAAYLRMINEKGGINGRKLNLIQYDDAYSPPKAVEQVRKLVEGDEVLFTFQII
HHCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEEE
GTPSNAAVQKYLNAKKVPQLLASTGASRFTDPQNFPWTIAYNPNYQSEGRIYAKYILANH
ECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEEEECC
PNAKIGILYQNDDLGRDYIAGLRSGLGAKADSMIVSEASYELSDPTVDSQIVKLKSSGAD
CCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCHHEEEECCCCC
LLYNASTPKFAAQAIKKLADLGWKPVHILDINASPVSATLKPAGLDISKDIISTNYGKDP
EEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEECCCCCCHHHHHHHCCCCCCC
ADPQWKDDPGVKAYFEFMDKYYPEGDKLNTVNTYGYSTAELLVQILRQCGDDLTRENVMR
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHH
QAANIKGFVPSLALPGLSINTSPTDFRINKQMQMMRFNGERWELFGPIIEDTGAAG
HHHCCCCCCHHHCCCCCCCCCCCCCEEECCHHEEHHCCCCEEEEECCHHHCCCCCC
>Mature Secondary Structure
MRTSLFGLVGFKAWAIALAASLTVALPATSAYAQKKYDTGATDTEIKIGQTVPFSGPASA
CCCCHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHCCCCCCCCEEEECCCCCCCCCHHH
YAGIGKTQAAYLRMINEKGGINGRKLNLIQYDDAYSPPKAVEQVRKLVEGDEVLFTFQII
HHCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEEE
GTPSNAAVQKYLNAKKVPQLLASTGASRFTDPQNFPWTIAYNPNYQSEGRIYAKYILANH
ECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEEEECC
PNAKIGILYQNDDLGRDYIAGLRSGLGAKADSMIVSEASYELSDPTVDSQIVKLKSSGAD
CCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCHHEEEECCCCC
LLYNASTPKFAAQAIKKLADLGWKPVHILDINASPVSATLKPAGLDISKDIISTNYGKDP
EEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEECCCCCCHHHHHHHCCCCCCC
ADPQWKDDPGVKAYFEFMDKYYPEGDKLNTVNTYGYSTAELLVQILRQCGDDLTRENVMR
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHH
QAANIKGFVPSLALPGLSINTSPTDFRINKQMQMMRFNGERWELFGPIIEDTGAAG
HHHCCCCCCHHHCCCCCCCCCCCCCEEECCHHEEHHCCCCEEEEECCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA