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The map label for this gene is amtB1 [H]

Identifier: 146337464

GI number: 146337464

Start: 321252

End: 322562

Strand: Reverse

Name: amtB1 [H]

Synonym: BRADO0307

Alternate gene names: 146337464

Gene position: 322562-321252 (Counterclockwise)

Preceding gene: 146337465

Following gene: 146337463

Centisome position: 4.33

GC content: 67.96

Gene sequence:

>1311_bases
ATGGGGACCTGCGCGTCTCGTTGCCTGCGCACGTCTGTAGCTTTGGCTGCAGCAGGCGCACTCGTCTTCGCCACGCCAGC
ATCGGCAGCCGCGCCGTCCACGATCGATGCGGCCGACACCGCCTGGATGATCGTCGCCACCGCGCTGGTGCTGATGATGA
CCATTCCGGGCCTGGCGCTGTTCTACTCGGGCATGGTGCGCAAGAAGAACGTGCTGGCGACGATGGCGCAGAGCCTCGCG
GCCGTCATGCTGATCTCGATCTTGTGGGTCGCGTTCGGCTATTCGCTCGCCTTCGTCGGCGACGGCGCCTGGATCGGCTC
GCTCGACCGCGCCTTCCTGGCCGGCATGGGGATGGACAGCGTGCATCCCGGCGCCAAGACGATCCCGGAGGCGCTTTTCA
TGCTGTACCAGATGACCTTCGCGATCATCACGGTGGCGCTGGTGGCGGGCTCGGTCGCCGACCGCATGCGCTTCTCGGCC
TATCTCGTGTTCGGCGTCGCCTGGTTCATCTTCGTCTATGTGCCGCTGGCGCATTGGATCTGGGGCGGCGGTTTCCTCGC
CCAGGCCGGCGTGGTCGACTTCGCCGGCGGCCTCGTGGTGCATCTGTCGGCCGGAACCGGCGGGCTGGTCGCGGCGCTGG
TGATGGGCCGCCGCCACGGCTATGGCAGCGAGAATCTGTCCCCCTTCGACCTGTCGCTCGCGGTGGTCGGCACCGGCCTG
CTGTGGGTCGGCTGGTTCGGCTTCAATGGCGGCTCGGCGCTCGGCGCCAACGCCCACGCCGTGATGGCGATCCTGACCAC
CCATCTGGCCGCCTGCGCCGGCGCAGTTACCTGGGGCGCGCTGGAATGGTCCACCCGGCGCAAGCCGTCGGTGCTCGGCA
TGATTTCTGGCGCGGTCGCCGGCCTTGGCACCATCACCCCTGCATCGGGCTTCGTGGCGCCGTGGCATGGCCTCGTCATC
GGCGCGCTCGCCGGCATCATCTGCTACTGGGCCTGCACTTCGCTGAAACACCGCTTCAATTATGACGACTCCCTCGACGT
GTTCGGCGTTCACGGCATCGGCGGCCTCACCGGCACGGTGCTGGCCGGCGTATTCGCGACCGCTGCGATCGGCGGCACGT
CCGGGCTCATCGAAGGCCATCCGATGCAGCTCGCGGCGCAGCTCTATGGGGTGGCTGTCACCCTGGTCTGGTCGGGCGGA
GTGACCTGGGCCCTGCTGAAGCTCGTCAGCGCCTTCGTGCCGCTGCGGGTGTCCCGCGAGCAGGAACTCGAGGGGCTCGA
CATCTCCCAGCATGGCGAGGCACTGCAATAA

Upstream 100 bases:

>100_bases
AGCGACGCGCTGTAAGTCGCCGGCTTCGCCGATCGTCTGCCGCAACGGGGCGGCTGGACCAACATTCCTGATTCTGAACT
GACATGCCGGGGGGACTGTC

Downstream 100 bases:

>100_bases
TTGCAGGATTTGGCGCCATTTGACCCGTTCTGAGTCGGCGAAACCTGCCCGCGCAGTGAGCAGCATTGTGTCTTGAGCCT
GGCATGCGCATTTTTTGCGC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MGTCASRCLRTSVALAAAGALVFATPASAAAPSTIDAADTAWMIVATALVLMMTIPGLALFYSGMVRKKNVLATMAQSLA
AVMLISILWVAFGYSLAFVGDGAWIGSLDRAFLAGMGMDSVHPGAKTIPEALFMLYQMTFAIITVALVAGSVADRMRFSA
YLVFGVAWFIFVYVPLAHWIWGGGFLAQAGVVDFAGGLVVHLSAGTGGLVAALVMGRRHGYGSENLSPFDLSLAVVGTGL
LWVGWFGFNGGSALGANAHAVMAILTTHLAACAGAVTWGALEWSTRRKPSVLGMISGAVAGLGTITPASGFVAPWHGLVI
GALAGIICYWACTSLKHRFNYDDSLDVFGVHGIGGLTGTVLAGVFATAAIGGTSGLIEGHPMQLAAQLYGVAVTLVWSGG
VTWALLKLVSAFVPLRVSREQELEGLDISQHGEALQ

Sequences:

>Translated_436_residues
MGTCASRCLRTSVALAAAGALVFATPASAAAPSTIDAADTAWMIVATALVLMMTIPGLALFYSGMVRKKNVLATMAQSLA
AVMLISILWVAFGYSLAFVGDGAWIGSLDRAFLAGMGMDSVHPGAKTIPEALFMLYQMTFAIITVALVAGSVADRMRFSA
YLVFGVAWFIFVYVPLAHWIWGGGFLAQAGVVDFAGGLVVHLSAGTGGLVAALVMGRRHGYGSENLSPFDLSLAVVGTGL
LWVGWFGFNGGSALGANAHAVMAILTTHLAACAGAVTWGALEWSTRRKPSVLGMISGAVAGLGTITPASGFVAPWHGLVI
GALAGIICYWACTSLKHRFNYDDSLDVFGVHGIGGLTGTVLAGVFATAAIGGTSGLIEGHPMQLAAQLYGVAVTLVWSGG
VTWALLKLVSAFVPLRVSREQELEGLDISQHGEALQ
>Mature_435_residues
GTCASRCLRTSVALAAAGALVFATPASAAAPSTIDAADTAWMIVATALVLMMTIPGLALFYSGMVRKKNVLATMAQSLAA
VMLISILWVAFGYSLAFVGDGAWIGSLDRAFLAGMGMDSVHPGAKTIPEALFMLYQMTFAIITVALVAGSVADRMRFSAY
LVFGVAWFIFVYVPLAHWIWGGGFLAQAGVVDFAGGLVVHLSAGTGGLVAALVMGRRHGYGSENLSPFDLSLAVVGTGLL
WVGWFGFNGGSALGANAHAVMAILTTHLAACAGAVTWGALEWSTRRKPSVLGMISGAVAGLGTITPASGFVAPWHGLVIG
ALAGIICYWACTSLKHRFNYDDSLDVFGVHGIGGLTGTVLAGVFATAAIGGTSGLIEGHPMQLAAQLYGVAVTLVWSGGV
TWALLKLVSAFVPLRVSREQELEGLDISQHGEALQ

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=406, Percent_Identity=46.0591133004926, Blast_Score=347, Evalue=7e-97,
Organism=Caenorhabditis elegans, GI32564042, Length=424, Percent_Identity=27.5943396226415, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17550180, Length=429, Percent_Identity=26.1072261072261, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17550182, Length=434, Percent_Identity=28.8018433179723, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI133931100, Length=429, Percent_Identity=26.5734265734266, Blast_Score=107, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6324187, Length=421, Percent_Identity=38.2422802850356, Blast_Score=247, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6325396, Length=418, Percent_Identity=33.2535885167464, Blast_Score=219, Evalue=6e-58,
Organism=Saccharomyces cerevisiae, GI6321559, Length=419, Percent_Identity=33.1742243436754, Blast_Score=216, Evalue=5e-57,
Organism=Drosophila melanogaster, GI161078314, Length=415, Percent_Identity=29.1566265060241, Blast_Score=138, Evalue=9e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 45029; Mature: 44898

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTCASRCLRTSVALAAAGALVFATPASAAAPSTIDAADTAWMIVATALVLMMTIPGLAL
CCHHHHHHHHHHHHHHHHCHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FYSGMVRKKNVLATMAQSLAAVMLISILWVAFGYSLAFVGDGAWIGSLDRAFLAGMGMDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHCCCCCC
VHPGAKTIPEALFMLYQMTFAIITVALVAGSVADRMRFSAYLVFGVAWFIFVYVPLAHWI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WGGGFLAQAGVVDFAGGLVVHLSAGTGGLVAALVMGRRHGYGSENLSPFDLSLAVVGTGL
HCCHHHHHCCHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCH
LWVGWFGFNGGSALGANAHAVMAILTTHLAACAGAVTWGALEWSTRRKPSVLGMISGAVA
HEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
GLGTITPASGFVAPWHGLVIGALAGIICYWACTSLKHRFNYDDSLDVFGVHGIGGLTGTV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHH
LAGVFATAAIGGTSGLIEGHPMQLAAQLYGVAVTLVWSGGVTWALLKLVSAFVPLRVSRE
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHCCCCCCH
QELEGLDISQHGEALQ
HHCCCCCCCCCCCCCC
>Mature Secondary Structure 
GTCASRCLRTSVALAAAGALVFATPASAAAPSTIDAADTAWMIVATALVLMMTIPGLAL
CHHHHHHHHHHHHHHHHCHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FYSGMVRKKNVLATMAQSLAAVMLISILWVAFGYSLAFVGDGAWIGSLDRAFLAGMGMDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHCCCCCC
VHPGAKTIPEALFMLYQMTFAIITVALVAGSVADRMRFSAYLVFGVAWFIFVYVPLAHWI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WGGGFLAQAGVVDFAGGLVVHLSAGTGGLVAALVMGRRHGYGSENLSPFDLSLAVVGTGL
HCCHHHHHCCHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCH
LWVGWFGFNGGSALGANAHAVMAILTTHLAACAGAVTWGALEWSTRRKPSVLGMISGAVA
HEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
GLGTITPASGFVAPWHGLVIGALAGIICYWACTSLKHRFNYDDSLDVFGVHGIGGLTGTV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHH
LAGVFATAAIGGTSGLIEGHPMQLAAQLYGVAVTLVWSGGVTWALLKLVSAFVPLRVSRE
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHCCCCCCH
QELEGLDISQHGEALQ
HHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]