Click here to switch to the map view.

The map label for this gene is glnB [H]

Identifier: 146337463

GI number: 146337463

Start: 320582

End: 320833

Strand: Reverse

Name: glnB [H]

Synonym: BRADO0306

Alternate gene names: 146337463

Gene position: 320833-320582 (Counterclockwise)

Preceding gene: 146337464

Following gene: 146337462

Centisome position: 4.3

GC content: 61.51

Gene sequence:

>252_bases
GTGACGGAAGTAAAGGGGTACGGCCGTCAGAAAGGTCATACGGAAATCTATCGCGGCGCTGAATACGCGGTGAGCTTTCT
GCCCAAGATCAAGATCGAGGTCGCGATCCCCTCCGACCAGGTCGACAAGACCATCGACGCGATCTCCTCGGCCGCCAAGA
CCGGTCAGATCGGCGACGGCAAGATCTTCGTCATCAGCCTCGACCACGCCGTGCGCATCCGCACCGGCGAAGCGGACGCT
GCGGCTCTCTGA

Upstream 100 bases:

>100_bases
GGGGATAGGACCCATGAAAATTGTTATGGCGATCATTAAGCCATTCAAGCTCGAAGAAGTCCGGGACGCCCTGACCGCCA
TCGGCGTTCATGGTCTCACG

Downstream 100 bases:

>100_bases
TTTCGCGCTCAAACCAGAACACTTACGGAGTAGAATAAAATGACGTTCAAGCGTCCAACCAGCGCGGGATGGGTCACGCT
CGCAATTGCCGGGCTCTGCA

Product: regulatory protein (P-II 2) for nitrogen assimilation

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 83; Mature: 82

Protein sequence:

>83_residues
MTEVKGYGRQKGHTEIYRGAEYAVSFLPKIKIEVAIPSDQVDKTIDAISSAAKTGQIGDGKIFVISLDHAVRIRTGEADA
AAL

Sequences:

>Translated_83_residues
MTEVKGYGRQKGHTEIYRGAEYAVSFLPKIKIEVAIPSDQVDKTIDAISSAAKTGQIGDGKIFVISLDHAVRIRTGEADA
AAL
>Mature_82_residues
TEVKGYGRQKGHTEIYRGAEYAVSFLPKIKIEVAIPSDQVDKTIDAISSAAKTGQIGDGKIFVISLDHAVRIRTGEADAA
AL

Specific function: In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P- II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deur

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1786655, Length=83, Percent_Identity=69.8795180722892, Blast_Score=124, Evalue=1e-30,
Organism=Escherichia coli, GI1788904, Length=83, Percent_Identity=63.855421686747, Blast_Score=117, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 8889; Mature: 8758

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: PS00496 PII_GLNB_UMP ; PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEVKGYGRQKGHTEIYRGAEYAVSFLPKIKIEVAIPSDQVDKTIDAISSAAKTGQIGDG
CCCCCCCCCCCCCCHHHCCHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCC
KIFVISLDHAVRIRTGEADAAAL
EEEEEECCCEEEEEECCCCCCCC
>Mature Secondary Structure 
TEVKGYGRQKGHTEIYRGAEYAVSFLPKIKIEVAIPSDQVDKTIDAISSAAKTGQIGDG
CCCCCCCCCCCCCHHHCCHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCC
KIFVISLDHAVRIRTGEADAAAL
EEEEEECCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA