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The map label for this gene is glnK1 [H]

Identifier: 146337465

GI number: 146337465

Start: 322648

End: 322986

Strand: Reverse

Name: glnK1 [H]

Synonym: BRADO0308

Alternate gene names: 146337465

Gene position: 322986-322648 (Counterclockwise)

Preceding gene: 146337467

Following gene: 146337464

Centisome position: 4.33

GC content: 64.01

Gene sequence:

>339_bases
ATGAAGCTCGTCGTCGCGATCATCAAACCCTTCAAGCTCGACGAGGTGCGCCAGGCCCTCACCGCGATCGGCGTGCACGG
CATGACCGTGACCGAGGTCAAGGGCTACGGCCGGCAGAAGGGCCACACCGAGATCTATCGCGGCGCCGAATACATCGTGA
ACTTCCTGCCCAAGCTGCGCATCGAGATCGCGGTCAATTCCGACATCGCCGACAAGGCGGTCGAGGTCATCACAACGCAT
GCGCGGACCGGCCAGATCGGCGACGGCAAGATCTTCGTGACGCCGATCGATCACGCCCGCCGCATCCGCACCGGCGAGAC
CGACAGCGACGCGCTGTAA

Upstream 100 bases:

>100_bases
GATTTTGTGCCATTTGCCTAGGAAGAGGCCAGAACGGCCCTTCAAAGCATGCCTTCCAAACAGGCTGCTCCGATCGACCT
GAGGAAACCAAGGCCCGACC

Downstream 100 bases:

>100_bases
GTCGCCGGCTTCGCCGATCGTCTGCCGCAACGGGGCGGCTGGACCAACATTCCTGATTCTGAACTGACATGCCGGGGGGA
CTGTCATGGGGACCTGCGCG

Product: regulatory protein (P-II 2) for nitrogen assimilation

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 112; Mature: 112

Protein sequence:

>112_residues
MKLVVAIIKPFKLDEVRQALTAIGVHGMTVTEVKGYGRQKGHTEIYRGAEYIVNFLPKLRIEIAVNSDIADKAVEVITTH
ARTGQIGDGKIFVTPIDHARRIRTGETDSDAL

Sequences:

>Translated_112_residues
MKLVVAIIKPFKLDEVRQALTAIGVHGMTVTEVKGYGRQKGHTEIYRGAEYIVNFLPKLRIEIAVNSDIADKAVEVITTH
ARTGQIGDGKIFVTPIDHARRIRTGETDSDAL
>Mature_112_residues
MKLVVAIIKPFKLDEVRQALTAIGVHGMTVTEVKGYGRQKGHTEIYRGAEYIVNFLPKLRIEIAVNSDIADKAVEVITTH
ARTGQIGDGKIFVTPIDHARRIRTGETDSDAL

Specific function: In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P- II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deur

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1786655, Length=112, Percent_Identity=60.7142857142857, Blast_Score=149, Evalue=4e-38,
Organism=Escherichia coli, GI1788904, Length=112, Percent_Identity=64.2857142857143, Blast_Score=147, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 12355; Mature: 12355

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS00496 PII_GLNB_UMP ; PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVVAIIKPFKLDEVRQALTAIGVHGMTVTEVKGYGRQKGHTEIYRGAEYIVNFLPKLR
CEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCEE
IEIAVNSDIADKAVEVITTHARTGQIGDGKIFVTPIDHARRIRTGETDSDAL
EEEEECCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKLVVAIIKPFKLDEVRQALTAIGVHGMTVTEVKGYGRQKGHTEIYRGAEYIVNFLPKLR
CEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCEE
IEIAVNSDIADKAVEVITTHARTGQIGDGKIFVTPIDHARRIRTGETDSDAL
EEEEECCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA