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The map label for this gene is hypF [H]

Identifier: 146301649

GI number: 146301649

Start: 4656065

End: 4658335

Strand: Direct

Name: hypF [H]

Synonym: Fjoh_3910

Alternate gene names: 146301649

Gene position: 4656065-4658335 (Clockwise)

Preceding gene: 146301648

Following gene: 146301650

Centisome position: 76.37

GC content: 31.79

Gene sequence:

>2271_bases
TTGACACCAACATTTCAAATAACAATTTCAGGCATTGTACAAGGTGTTGGTTTTCGGCCTTTTGTATACAATACTGCCAT
TCAGTTTAACCTGAAAGGCTTTGTTTCTAATAATGAATTTGGTGTTGTTGTAATTGTGCAGGAAGAAGAAAAAACAATAA
GAGAATTTGTAGCCCAACTAAAAAAAAGACATCCAAAAAAAGCGATAATTAATACTATTCAGGTTTCAGAAATTGAAACG
GAAGAAAGATTTGACCTCTTTTTTATAAAACCAACAGAAAAAGGCATTTCGATCAACGCGCCTTTGACACCCGATTTTGC
TATATGTAAAAACTGTAAAGAAGAAATTCTGGATCCGCAAAACCGACGCTTTTATTATCCGTTTACAAGTTGTACGTCAT
GCGGACCCAGATATGCCATTGCTGAAAAATTTCCTTTTGAAAGAGAAAATACAAGTATGAGCCCATTCAAAATGTGCCAA
ACTTGTCTGGAAGAATACCTCAATCCAAACGATATACGTTTTCATTCGCAGACCAATTCATGTCCTGAATGCGGTATTCA
GATTGTATTTACAGATAATAATGGAACTCTTATTTCTGGAACAAACAAAGAAATTTTCGAATCTATTTCAGAGAAATTAA
ATCAGGGACAAATAATTGCCGTAAAAAATACCTCAGGTTATTTACTGCTTTGCGATGGTTCAAATAATCAAACTGTTCAG
GAATTACGAAATAGAAAAAGACGTTCAGCAAAACCTTTTGCTGTATTATTCTCTGATTTTAAACAAATTAAAAAGCATTT
ATTACATGATAAAACAGAAAAGAAACTTTTAAAATCGACACAGGCGCCTATTGTAATTTTACCCATAAAAAATCAAAAAA
AATTAGCTGTAAATGAAATAGCTCCCAATATGAAAACTATTGGAGCAATGCTTCCCAATTCAGGAATATTGCATTTAATT
TCTAATTTGTTTCAGAAACCATTAGTTGCAACGAGCGGTAATTTTAATGGTTCTCCCCTTTTTTTTAATCAAAATGAAGC
CGTTCAATCCTTAAACTCCATTGCAGATTATTATCTGCATCACAATCTTGAAATTCAAAACTCACAAGATGACTCGGTAA
TTCGGTTTTCGAGAAAAAACAAACAAAAAATTGTTTTGCGCCGTGCGAGAGGTCTGGCGCCAAATATTGATAATTTTCAT
CTGAATAATACTTCAGACAAAATACTCTGTTTAGGAGCAGCATTAAAAAACACAATTACCATTTCAAACCAGCAAATATA
TCAAAGTGAATATATTGGAAATGTCTTGAATTATGATACGTATAAAAGGTTTGAAAATAGAATACAAAACTATCAGCATT
TTTTTGATTTCGTTCCGCAGACAATAGTTTACGATCAGCATCCGAGTTACGATAACAATAAAATTGTTTCTGATTTTTTA
AAAGAAAATCCTTTACTGAAAACTATAAAAGTTCAGCATCACGAAGCGCATTTTGCAGCGATTTTGGGAGAAAAAAAGCT
TTGGAAAAAGTCTAAAATTCTAGGCGTTATTTGGGACGGTTCTGGATTTGGAAATGAAAACGAAATTTATGGCGGAGAAT
TTTTTAAATACAATCGCGACGAAATTACCCGAATAGGGCATTTAGAATATTTCCCCTGGATTTTAGGAGATAAAATGGCG
TTAAATCCAAAAATGTCGGCGCTTTCTATCAGTCAAAATGATGATTATTTTCAGTCTTTTTTCAATAAAAACGAATTAAC
AGTTTACAGTAAACTTATTGAAAACAGTGTTATAAAAACTTCTTCGATAGGAAGATTATTTGATGCTGTTGCTTTTACAT
TGGGTTTTAAAGGAAACATTTCTTTTGAAGGTCAAACGGGTATGTTTTTAGAAAATCTGGCACAAAAGGCTTTTTACAAA
TCAAACGTAAAACTAAAGGATTATTTAAAGGATGAAAACATAACGAATATAATCCCGTCAAAAAAACTGCTGGTGCAGAT
CGTTAAAGCTTTTCAAAACAATATTGACTGTAATGTTATTGCTTTAAACTTTCATCACACTTTAGTGAAGTGCATTGAAA
AAACAGCTTTGTTTTCAAAATCAAAAGAAATTGCTTTCAGCGGAGGCGTTTTTCAAAACTCTGTTCTGGTTGATTTAATT
ATAGATCATTTACAGCCAAATTACAAATTGCATTTTCATGAAACACTTTCTCCAAATGATGAAAATATTTCATTTGGACA
ACTCAATTATTATTTACATCTAAAAAAATAA

Upstream 100 bases:

>100_bases
GTGCATGCAGCTTTCACCTAAAGTAGAAGCTGCGATAGAAAAAGTAACGGCTGAAATTATACAGCAGATAGAAAAAATAA
AAATTTAATACCCTCTTTTC

Downstream 100 bases:

>100_bases
ATTATGGACATTTTAGAACTATTAGGACACATTGGTAAAACCGAAGAAAAAGATAAAATTTCGTATTCAGCTGGAGTTGT
AATGGCTCTAAGTCTGAAAG

Product: (NiFe) hydrogenase maturation protein HypF

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]

Number of amino acids: Translated: 756; Mature: 755

Protein sequence:

>756_residues
MTPTFQITISGIVQGVGFRPFVYNTAIQFNLKGFVSNNEFGVVVIVQEEEKTIREFVAQLKKRHPKKAIINTIQVSEIET
EERFDLFFIKPTEKGISINAPLTPDFAICKNCKEEILDPQNRRFYYPFTSCTSCGPRYAIAEKFPFERENTSMSPFKMCQ
TCLEEYLNPNDIRFHSQTNSCPECGIQIVFTDNNGTLISGTNKEIFESISEKLNQGQIIAVKNTSGYLLLCDGSNNQTVQ
ELRNRKRRSAKPFAVLFSDFKQIKKHLLHDKTEKKLLKSTQAPIVILPIKNQKKLAVNEIAPNMKTIGAMLPNSGILHLI
SNLFQKPLVATSGNFNGSPLFFNQNEAVQSLNSIADYYLHHNLEIQNSQDDSVIRFSRKNKQKIVLRRARGLAPNIDNFH
LNNTSDKILCLGAALKNTITISNQQIYQSEYIGNVLNYDTYKRFENRIQNYQHFFDFVPQTIVYDQHPSYDNNKIVSDFL
KENPLLKTIKVQHHEAHFAAILGEKKLWKKSKILGVIWDGSGFGNENEIYGGEFFKYNRDEITRIGHLEYFPWILGDKMA
LNPKMSALSISQNDDYFQSFFNKNELTVYSKLIENSVIKTSSIGRLFDAVAFTLGFKGNISFEGQTGMFLENLAQKAFYK
SNVKLKDYLKDENITNIIPSKKLLVQIVKAFQNNIDCNVIALNFHHTLVKCIEKTALFSKSKEIAFSGGVFQNSVLVDLI
IDHLQPNYKLHFHETLSPNDENISFGQLNYYLHLKK

Sequences:

>Translated_756_residues
MTPTFQITISGIVQGVGFRPFVYNTAIQFNLKGFVSNNEFGVVVIVQEEEKTIREFVAQLKKRHPKKAIINTIQVSEIET
EERFDLFFIKPTEKGISINAPLTPDFAICKNCKEEILDPQNRRFYYPFTSCTSCGPRYAIAEKFPFERENTSMSPFKMCQ
TCLEEYLNPNDIRFHSQTNSCPECGIQIVFTDNNGTLISGTNKEIFESISEKLNQGQIIAVKNTSGYLLLCDGSNNQTVQ
ELRNRKRRSAKPFAVLFSDFKQIKKHLLHDKTEKKLLKSTQAPIVILPIKNQKKLAVNEIAPNMKTIGAMLPNSGILHLI
SNLFQKPLVATSGNFNGSPLFFNQNEAVQSLNSIADYYLHHNLEIQNSQDDSVIRFSRKNKQKIVLRRARGLAPNIDNFH
LNNTSDKILCLGAALKNTITISNQQIYQSEYIGNVLNYDTYKRFENRIQNYQHFFDFVPQTIVYDQHPSYDNNKIVSDFL
KENPLLKTIKVQHHEAHFAAILGEKKLWKKSKILGVIWDGSGFGNENEIYGGEFFKYNRDEITRIGHLEYFPWILGDKMA
LNPKMSALSISQNDDYFQSFFNKNELTVYSKLIENSVIKTSSIGRLFDAVAFTLGFKGNISFEGQTGMFLENLAQKAFYK
SNVKLKDYLKDENITNIIPSKKLLVQIVKAFQNNIDCNVIALNFHHTLVKCIEKTALFSKSKEIAFSGGVFQNSVLVDLI
IDHLQPNYKLHFHETLSPNDENISFGQLNYYLHLKK
>Mature_755_residues
TPTFQITISGIVQGVGFRPFVYNTAIQFNLKGFVSNNEFGVVVIVQEEEKTIREFVAQLKKRHPKKAIINTIQVSEIETE
ERFDLFFIKPTEKGISINAPLTPDFAICKNCKEEILDPQNRRFYYPFTSCTSCGPRYAIAEKFPFERENTSMSPFKMCQT
CLEEYLNPNDIRFHSQTNSCPECGIQIVFTDNNGTLISGTNKEIFESISEKLNQGQIIAVKNTSGYLLLCDGSNNQTVQE
LRNRKRRSAKPFAVLFSDFKQIKKHLLHDKTEKKLLKSTQAPIVILPIKNQKKLAVNEIAPNMKTIGAMLPNSGILHLIS
NLFQKPLVATSGNFNGSPLFFNQNEAVQSLNSIADYYLHHNLEIQNSQDDSVIRFSRKNKQKIVLRRARGLAPNIDNFHL
NNTSDKILCLGAALKNTITISNQQIYQSEYIGNVLNYDTYKRFENRIQNYQHFFDFVPQTIVYDQHPSYDNNKIVSDFLK
ENPLLKTIKVQHHEAHFAAILGEKKLWKKSKILGVIWDGSGFGNENEIYGGEFFKYNRDEITRIGHLEYFPWILGDKMAL
NPKMSALSISQNDDYFQSFFNKNELTVYSKLIENSVIKTSSIGRLFDAVAFTLGFKGNISFEGQTGMFLENLAQKAFYKS
NVKLKDYLKDENITNIIPSKKLLVQIVKAFQNNIDCNVIALNFHHTLVKCIEKTALFSKSKEIAFSGGVFQNSVLVDLII
DHLQPNYKLHFHETLSPNDENISFGQLNYYLHLKK

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=764, Percent_Identity=31.9371727748691, Blast_Score=331, Evalue=1e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 86518; Mature: 86387

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPTFQITISGIVQGVGFRPFVYNTAIQFNLKGFVSNNEFGVVVIVQEEEKTIREFVAQL
CCCEEEEEEHHHHCCCCCCCEEEEEEEEEEEEEEECCCCEEEEEEEECCHHHHHHHHHHH
KKRHPKKAIINTIQVSEIETEERFDLFFIKPTEKGISINAPLTPDFAICKNCKEEILDPQ
HHCCCHHHHHHEEEEEECCCCCCEEEEEEEECCCCEEECCCCCCCHHHHHHHHHHHCCCC
NRRFYYPFTSCTSCGPRYAIAEKFPFERENTSMSPFKMCQTCLEEYLNPNDIRFHSQTNS
CCEEEECCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCC
CPECGIQIVFTDNNGTLISGTNKEIFESISEKLNQGQIIAVKNTSGYLLLCDGSNNQTVQ
CCCCCEEEEEECCCCEEEECCCHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCHHHH
ELRNRKRRSAKPFAVLFSDFKQIKKHLLHDKTEKKLLKSTQAPIVILPIKNQKKLAVNEI
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCCCCEEHHHH
APNMKTIGAMLPNSGILHLISNLFQKPLVATSGNFNGSPLFFNQNEAVQSLNSIADYYLH
CCCHHHHHHHCCCCCHHHHHHHHHHCCCEEECCCCCCCCEEECCCHHHHHHHHHHHHHHH
HNLEIQNSQDDSVIRFSRKNKQKIVLRRARGLAPNIDNFHLNNTSDKILCLGAALKNTIT
CCEEECCCCCCCEEEECCCCHHHHHHHHHCCCCCCCCCEEECCCCCCEEEEEEHHCCEEE
ISNQQIYQSEYIGNVLNYDTYKRFENRIQNYQHFFDFVPQTIVYDQHPSYDNNKIVSDFL
ECCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCCCCCHHHHHHH
KENPLLKTIKVQHHEAHFAAILGEKKLWKKSKILGVIWDGSGFGNENEIYGGEFFKYNRD
HCCCCEEEEEEECCHHHHHHHHCCHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEECHH
EITRIGHLEYFPWILGDKMALNPKMSALSISQNDDYFQSFFNKNELTVYSKLIENSVIKT
HHHEECCCCCCCHHHCCCEECCCCCCEEEECCCCHHHHHHCCCCCHHHHHHHHHCCCCCH
SSIGRLFDAVAFTLGFKGNISFEGQTGMFLENLAQKAFYKSNVKLKDYLKDENITNIIPS
HHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCC
KKLLVQIVKAFQNNIDCNVIALNFHHTLVKCIEKTALFSKSKEIAFSGGVFQNSVLVDLI
HHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHH
IDHLQPNYKLHFHETLSPNDENISFGQLNYYLHLKK
HHHCCCCEEEEEEECCCCCCCCEEEEEEEEEEEECC
>Mature Secondary Structure 
TPTFQITISGIVQGVGFRPFVYNTAIQFNLKGFVSNNEFGVVVIVQEEEKTIREFVAQL
CCEEEEEEHHHHCCCCCCCEEEEEEEEEEEEEEECCCCEEEEEEEECCHHHHHHHHHHH
KKRHPKKAIINTIQVSEIETEERFDLFFIKPTEKGISINAPLTPDFAICKNCKEEILDPQ
HHCCCHHHHHHEEEEEECCCCCCEEEEEEEECCCCEEECCCCCCCHHHHHHHHHHHCCCC
NRRFYYPFTSCTSCGPRYAIAEKFPFERENTSMSPFKMCQTCLEEYLNPNDIRFHSQTNS
CCEEEECCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCC
CPECGIQIVFTDNNGTLISGTNKEIFESISEKLNQGQIIAVKNTSGYLLLCDGSNNQTVQ
CCCCCEEEEEECCCCEEEECCCHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCHHHH
ELRNRKRRSAKPFAVLFSDFKQIKKHLLHDKTEKKLLKSTQAPIVILPIKNQKKLAVNEI
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCCCCEEHHHH
APNMKTIGAMLPNSGILHLISNLFQKPLVATSGNFNGSPLFFNQNEAVQSLNSIADYYLH
CCCHHHHHHHCCCCCHHHHHHHHHHCCCEEECCCCCCCCEEECCCHHHHHHHHHHHHHHH
HNLEIQNSQDDSVIRFSRKNKQKIVLRRARGLAPNIDNFHLNNTSDKILCLGAALKNTIT
CCEEECCCCCCCEEEECCCCHHHHHHHHHCCCCCCCCCEEECCCCCCEEEEEEHHCCEEE
ISNQQIYQSEYIGNVLNYDTYKRFENRIQNYQHFFDFVPQTIVYDQHPSYDNNKIVSDFL
ECCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCCCCCHHHHHHH
KENPLLKTIKVQHHEAHFAAILGEKKLWKKSKILGVIWDGSGFGNENEIYGGEFFKYNRD
HCCCCEEEEEEECCHHHHHHHHCCHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEECHH
EITRIGHLEYFPWILGDKMALNPKMSALSISQNDDYFQSFFNKNELTVYSKLIENSVIKT
HHHEECCCCCCCHHHCCCEECCCCCCEEEECCCCHHHHHHCCCCCHHHHHHHHHCCCCCH
SSIGRLFDAVAFTLGFKGNISFEGQTGMFLENLAQKAFYKSNVKLKDYLKDENITNIIPS
HHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCC
KKLLVQIVKAFQNNIDCNVIALNFHHTLVKCIEKTALFSKSKEIAFSGGVFQNSVLVDLI
HHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHH
IDHLQPNYKLHFHETLSPNDENISFGQLNYYLHLKK
HHHCCCCEEEEEEECCCCCCCCEEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]