Click here to switch to the map view.

The map label for this gene is kojP [H]

Identifier: 146300396

GI number: 146300396

Start: 3143243

End: 3144430

Strand: Direct

Name: kojP [H]

Synonym: Fjoh_2641

Alternate gene names: 146300396

Gene position: 3143243-3144430 (Clockwise)

Preceding gene: 146300393

Following gene: 146300404

Centisome position: 51.56

GC content: 42.34

Gene sequence:

>1188_bases
TTGAGGAATAGCCTCTGGCAAAGTGATATTCAAATAGAAGGAGATCCGCAGGCTCAGCAGGATGTTAGAAGCATGCTTTA
TCATCTTTATTCTTTCACAAGAAAAAGCACATCTCTTTCTCCGTCGCCAATGGGGCTCAGCGGGCTGGGATATAACGGAC
ATGTGTTTTGGGATACCGAAATCTGGATGTTTCCTCCCATGCTGCTGCTCCATCCTGAAATAGCAAAAAGCATGATTGAG
TATCGTTTTCAGAGGCTGGATGCAGCCCGTAAAAAAGCTGCTTTATATGGATATGACGGGGCAATGTATCCTTGGGAAAG
CGCAGATTCAGGATCTGAAGAAACTCCGGTCAATGCTTTAACGGGTGCATTTGAACACCATATCACGGGAGATGTTGCAA
TTGCTGCCTGGCAGTATTACCTGGTTACAGGGGACAAGGAATGGCTGAAAGAAAAAGGGTGGCCGATATTAAAAGCAACT
GCAGAATTTTGGGCGAGCAGAGTAGAGAAGAATAATAAAGGTGATTATGAAATAAAGAATGTCGTTGCAACAGATGAATG
GGCTGAGAATATCGACAACAATGCTTATACAAATGGAATAGCGATCCGAAATCTGCAGTACGCATCTAAATGCGCAGCTG
TATTGGGCATAACAGCTCCAAAAGAATGGAATCTGATTGCTGCTAAAATCCCAATTTCAAAAATGGGCAATGGCGTGACA
AGAGAACATGACAGCTATTCGGATCAAAGTATCAAGCAGGCTGACGTGAATCTGCTGGCATATCCATTGCAGATCATAAC
TGACAGAAATCAAATAGAAAAGGATTTAAAATTTTATGAAACTAAAATACCCCATTCCGATACACCAGCTATGACACAGG
CCATATTTTCACTGCTATACAGCCGTTTAGGAGACAGCGATCAGGCTTATCATTGGTTTAAAGATGCCTATGAGCCAAAT
CTTAATCCTCCCTTCAGGGTCATTGCAGAATGTAAAGGCGGTGAAAACCCTTATTTTGCAACCGGCGCCGGCGGTGTTCT
GCAGGCAGTCATGATGGGATTCGGAGGCTTGGATATAGATGGAGGCGGAGGTATTAAACAAGTTAAAACAGTACTGCCTA
AAAATTGGAAAAAATTAACAATAATGGGAGTTGGAACAGAAAAAAAGACTTTCATTAAAACTCAGTAA

Upstream 100 bases:

>100_bases
ACATTTTTTAGCATTGGAAGAAACATACTTTTTCTGGGCTAAAAATATAGCTTTGAATTTAATTTAGCGTAAGTAAAAAT
ACCTTATTTCAACCCGAATT

Downstream 100 bases:

>100_bases
ATTTTGTACCTTAATTAGTAAAAGTGTAAAAAAGGCGGTTTAGGTAATTCCAATACCGCCTTTTTTTGGTAGATTAGTTA
AAGTTTTTGTACTTAAATGG

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MRNSLWQSDIQIEGDPQAQQDVRSMLYHLYSFTRKSTSLSPSPMGLSGLGYNGHVFWDTEIWMFPPMLLLHPEIAKSMIE
YRFQRLDAARKKAALYGYDGAMYPWESADSGSEETPVNALTGAFEHHITGDVAIAAWQYYLVTGDKEWLKEKGWPILKAT
AEFWASRVEKNNKGDYEIKNVVATDEWAENIDNNAYTNGIAIRNLQYASKCAAVLGITAPKEWNLIAAKIPISKMGNGVT
REHDSYSDQSIKQADVNLLAYPLQIITDRNQIEKDLKFYETKIPHSDTPAMTQAIFSLLYSRLGDSDQAYHWFKDAYEPN
LNPPFRVIAECKGGENPYFATGAGGVLQAVMMGFGGLDIDGGGGIKQVKTVLPKNWKKLTIMGVGTEKKTFIKTQ

Sequences:

>Translated_395_residues
MRNSLWQSDIQIEGDPQAQQDVRSMLYHLYSFTRKSTSLSPSPMGLSGLGYNGHVFWDTEIWMFPPMLLLHPEIAKSMIE
YRFQRLDAARKKAALYGYDGAMYPWESADSGSEETPVNALTGAFEHHITGDVAIAAWQYYLVTGDKEWLKEKGWPILKAT
AEFWASRVEKNNKGDYEIKNVVATDEWAENIDNNAYTNGIAIRNLQYASKCAAVLGITAPKEWNLIAAKIPISKMGNGVT
REHDSYSDQSIKQADVNLLAYPLQIITDRNQIEKDLKFYETKIPHSDTPAMTQAIFSLLYSRLGDSDQAYHWFKDAYEPN
LNPPFRVIAECKGGENPYFATGAGGVLQAVMMGFGGLDIDGGGGIKQVKTVLPKNWKKLTIMGVGTEKKTFIKTQ
>Mature_395_residues
MRNSLWQSDIQIEGDPQAQQDVRSMLYHLYSFTRKSTSLSPSPMGLSGLGYNGHVFWDTEIWMFPPMLLLHPEIAKSMIE
YRFQRLDAARKKAALYGYDGAMYPWESADSGSEETPVNALTGAFEHHITGDVAIAAWQYYLVTGDKEWLKEKGWPILKAT
AEFWASRVEKNNKGDYEIKNVVATDEWAENIDNNAYTNGIAIRNLQYASKCAAVLGITAPKEWNLIAAKIPISKMGNGVT
REHDSYSDQSIKQADVNLLAYPLQIITDRNQIEKDLKFYETKIPHSDTPAMTQAIFSLLYSRLGDSDQAYHWFKDAYEPN
LNPPFRVIAECKGGENPYFATGAGGVLQAVMMGFGGLDIDGGGGIKQVKTVLPKNWKKLTIMGVGTEKKTFIKTQ

Specific function: Hydrolyzes kojibiose in the presence of an inorganic phosphoric acid to form D-glucose and beta-D-glucose-1-phosphoric acid. Can act with alpha-1,2-oligoglucans, such as selaginose, as substrate, but more slowly. Inactive when dissaccharides with linkages

COG id: COG1554

COG function: function code G; Trehalose and maltose hydrolases (possible phosphorylases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 65 family [H]

Homologues:

Organism=Homo sapiens, GI187829418, Length=374, Percent_Identity=35.2941176470588, Blast_Score=208, Evalue=8e-54,
Organism=Escherichia coli, GI1787575, Length=417, Percent_Identity=29.9760191846523, Blast_Score=179, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6325283, Length=412, Percent_Identity=33.495145631068, Blast_Score=176, Evalue=9e-45,
Organism=Drosophila melanogaster, GI24583760, Length=382, Percent_Identity=32.7225130890052, Blast_Score=174, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR011013
- InterPro:   IPR005194
- InterPro:   IPR005195
- InterPro:   IPR005196
- InterPro:   IPR017045 [H]

Pfam domain/function: PF03633 Glyco_hydro_65C; PF03632 Glyco_hydro_65m; PF03636 Glyco_hydro_65N [H]

EC number: =2.4.1.230 [H]

Molecular weight: Translated: 44291; Mature: 44291

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNSLWQSDIQIEGDPQAQQDVRSMLYHLYSFTRKSTSLSPSPMGLSGLGYNGHVFWDTE
CCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCC
IWMFPPMLLLHPEIAKSMIEYRFQRLDAARKKAALYGYDGAMYPWESADSGSEETPVNAL
HHHHCCHHEECHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCHHHH
TGAFEHHITGDVAIAAWQYYLVTGDKEWLKEKGWPILKATAEFWASRVEKNNKGDYEIKN
HHHHHHHCCCCEEEEEEEEEEEECCHHHHHHCCCCEEHHHHHHHHHHHHCCCCCCEEEEE
VVATDEWAENIDNNAYTNGIAIRNLQYASKCAAVLGITAPKEWNLIAAKIPISKMGNGVT
EEECHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHCCCCC
REHDSYSDQSIKQADVNLLAYPLQIITDRNQIEKDLKFYETKIPHSDTPAMTQAIFSLLY
CCCCCCCCCHHHHHCCHHHEEHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
SRLGDSDQAYHWFKDAYEPNLNPPFRVIAECKGGENPYFATGAGGVLQAVMMGFGGLDID
HHHCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCEEECCCCHHHHHHHHCCCCEEEC
GGGGIKQVKTVLPKNWKKLTIMGVGTEKKTFIKTQ
CCCCHHHHHHHCCCCCCEEEEEEECCCCCEEEECC
>Mature Secondary Structure
MRNSLWQSDIQIEGDPQAQQDVRSMLYHLYSFTRKSTSLSPSPMGLSGLGYNGHVFWDTE
CCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCC
IWMFPPMLLLHPEIAKSMIEYRFQRLDAARKKAALYGYDGAMYPWESADSGSEETPVNAL
HHHHCCHHEECHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCHHHH
TGAFEHHITGDVAIAAWQYYLVTGDKEWLKEKGWPILKATAEFWASRVEKNNKGDYEIKN
HHHHHHHCCCCEEEEEEEEEEEECCHHHHHHCCCCEEHHHHHHHHHHHHCCCCCCEEEEE
VVATDEWAENIDNNAYTNGIAIRNLQYASKCAAVLGITAPKEWNLIAAKIPISKMGNGVT
EEECHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHCCCCC
REHDSYSDQSIKQADVNLLAYPLQIITDRNQIEKDLKFYETKIPHSDTPAMTQAIFSLLY
CCCCCCCCCHHHHHCCHHHEEHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
SRLGDSDQAYHWFKDAYEPNLNPPFRVIAECKGGENPYFATGAGGVLQAVMMGFGGLDID
HHHCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCEEECCCCHHHHHHHHCCCCEEEC
GGGGIKQVKTVLPKNWKKLTIMGVGTEKKTFIKTQ
CCCCHHHHHHHCCCCCCEEEEEEECCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA