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The map label for this gene is cspE [H]

Identifier: 146300393

GI number: 146300393

Start: 3137498

End: 3137689

Strand: Direct

Name: cspE [H]

Synonym: Fjoh_2638

Alternate gene names: 146300393

Gene position: 3137498-3137689 (Clockwise)

Preceding gene: 146300390

Following gene: 146300396

Centisome position: 51.46

GC content: 40.62

Gene sequence:

>192_bases
ATGCAAGAAGGTACAGTAAAATTTTTCAATGAAGAAAAGGGATTCGGATTCATAACTCCTAATAATGGAGGAGCCGAAGT
TTTTGTTCATGCTTCAGGACTGTCAGAAAGCATCCGTCAAGACGATGCAGTACGATATGACATCGAAGAAGGCCGTAAAG
GTCCAAACGCAGTAAACGTAGTTGTAGCATAA

Upstream 100 bases:

>100_bases
AAACCCCGTGGACAGCCGTATAAATTATAGAAAATAAAAAACTCAAATTCAGGCGTTTCTAATTAAATATACCTATCTTT
GAATAATTAAATACATTAAA

Downstream 100 bases:

>100_bases
CCATACTATCACAGTGAGCTTGTAAAGGCGCCTGCTCAAGCAGGCGTCTTTTTTATTGGCAGAACTGATTATTTCCGAGC
GCCGCTTAAAGTATTTTTTA

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: CSP-E [H]

Number of amino acids: Translated: 63; Mature: 63

Protein sequence:

>63_residues
MQEGTVKFFNEEKGFGFITPNNGGAEVFVHASGLSESIRQDDAVRYDIEEGRKGPNAVNVVVA

Sequences:

>Translated_63_residues
MQEGTVKFFNEEKGFGFITPNNGGAEVFVHASGLSESIRQDDAVRYDIEEGRKGPNAVNVVVA
>Mature_63_residues
MQEGTVKFFNEEKGFGFITPNNGGAEVFVHASGLSESIRQDDAVRYDIEEGRKGPNAVNVVVA

Specific function: Unknown

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1788126, Length=63, Percent_Identity=47.6190476190476, Blast_Score=62, Evalue=6e-12,
Organism=Escherichia coli, GI2367114, Length=63, Percent_Identity=44.4444444444444, Blast_Score=62, Evalue=7e-12,
Organism=Escherichia coli, GI1787834, Length=62, Percent_Identity=50, Blast_Score=62, Evalue=1e-11,
Organism=Escherichia coli, GI1786841, Length=63, Percent_Identity=46.031746031746, Blast_Score=60, Evalue=2e-11,
Organism=Escherichia coli, GI1789979, Length=62, Percent_Identity=48.3870967741936, Blast_Score=59, Evalue=6e-11,

Paralogues:

None

Copy number: 8300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 6830; Mature: 6830

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEGTVKFFNEEKGFGFITPNNGGAEVFVHASGLSESIRQDDAVRYDIEEGRKGPNAVNV
CCCCCEEEEECCCCEEEECCCCCCEEEEEECCCCCCCCCCCCCEEEEHHHCCCCCCEEEE
VVA
EEC
>Mature Secondary Structure
MQEGTVKFFNEEKGFGFITPNNGGAEVFVHASGLSESIRQDDAVRYDIEEGRKGPNAVNV
CCCCCEEEEECCCCEEEECCCCCCEEEEEECCCCCCCCCCCCCEEEEHHHCCCCCCEEEE
VVA
EEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]