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The map label for this gene is rimK [H]

Identifier: 146300337

GI number: 146300337

Start: 3071366

End: 3072733

Strand: Reverse

Name: rimK [H]

Synonym: Fjoh_2582

Alternate gene names: 146300337

Gene position: 3072733-3071366 (Counterclockwise)

Preceding gene: 146300338

Following gene: 146300336

Centisome position: 50.4

GC content: 37.72

Gene sequence:

>1368_bases
ATGCTTCAAAACAAAGTCATTTTAGGCAGTGAAGAATGGTGCTCATTTCCAGAACTTGGAATCCCGACAATTAAAGCTCG
TGTCGATTCTGGTGCCAAAACTTCGGCAATGCATGCAATCAATATTGCTCCTTTTATAAAAAATGATGCTAATTGGGTAA
AATTTGATATTAACCCAATTCAAAATAACATTAAAACCATTATTCACTGTGAAGCCCCGCTGGTTGATAAACGAATTGTA
AAAAGTTCTAGTGGTTACAGAGAACATCGTTATGTTATTCAGACGCATTTAAAAATTGGCGATGCAAAATGGCCGATTGA
AATGACTTTGACCAATCGTGATTCGATGGGTTTCCGTATGCTTCTTGGACGCGAGGCCATGAGCGGAAGAGTTTTGGTAG
ATCCTGAACAAAAATATCTTTTAGGACAGCCTTCTCCGGAAACTTTAAAAGAATTATACCAAAACTCTGAAAAAGCAACT
ACAGGTTTAAGAATTGGTCTTTTGGCCAGTAATCCTGAATTATACAGTAACAAACGTATTATGGAAGCCGGCGAAATGCG
CGGCCACGAAATGCACTTTCTGAACATAAAGGAATGCTACATGAAACTTGATGCTAAAAAGCCCGAAATTCACTATCGAG
GCGGAAAAATTCTAAACGAATTTGACGCTATTATTCCAAGAATCCGTCCGAGTATTACTTTTTACGGCTGCGCATTAACA
CGTCAGTTTGAGGCTTTGAAGGTTTTTGTTTTAAATTCTGCCACAGCTATTACACAATCCAGAGATAAATTATATTCATT
ACAGCTTCTTTTGAACAGCGGAATTGATATTCCAACTACAGGATTTGCCAATTCTCCGCTTGATACAGACAATTTAATTA
AAATGGTGGGCGGTTCACCTCTAATTGTAAAATTATTAGAAGGAACACAAGGAAAAGGCGTTGTTTTAGCCGAAACCAAA
AAAGCTGCAGAAAGTGTAATCAATGCTTTTAAAAGTTTAAATGCCAACATCTTAGTTCAGGAATTTATTAAAGAAGCAAA
CGGAAAAGATATACGCTGTTTTGTAATCGACGGAAAAGTAGTTGCCGCTATTCAGCGCGAAGCAATGCCGGGCGAATTTA
GAGCTAATATACACCTTGGCGGAACAGCATCTGTAATCAAAGTAACTGCCGAAGAAAAAAAGATTGCCATAAAAGCCGCA
AAAGCAATGGATTTAAAAGTAGCCGGAGTTGATATTATTCGTTCTTCAAAAGGACCTTTATTGCTTGAAGTAAACTCTTC
TCCAGGGCTTGAAGGAATTGAGGGCGCAACAAACAAAGACGTTGCTGGAGAAATGATTAAAGCGATTGAGAAAAATTTCA
AACTATAA

Upstream 100 bases:

>100_bases
GTTTTCATAACATAACGGGTATTTTTTTTGCAAAAATCACTATCTTTAGTAAAAGCAAATAGCTATTTTTGAATTCAAAT
TCTATAATAAAAAACAAAAT

Downstream 100 bases:

>100_bases
TTAAGTTCATTTTAACTTAATCATCTCAAAATAATTTAGCTGCTTTGTCAAAGTTTAAAACCTTTGACAAAGCTTTTTTA
TTTAACTTTAAAATAAAAAG

Product: alpha-L-glutamate ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MLQNKVILGSEEWCSFPELGIPTIKARVDSGAKTSAMHAINIAPFIKNDANWVKFDINPIQNNIKTIIHCEAPLVDKRIV
KSSSGYREHRYVIQTHLKIGDAKWPIEMTLTNRDSMGFRMLLGREAMSGRVLVDPEQKYLLGQPSPETLKELYQNSEKAT
TGLRIGLLASNPELYSNKRIMEAGEMRGHEMHFLNIKECYMKLDAKKPEIHYRGGKILNEFDAIIPRIRPSITFYGCALT
RQFEALKVFVLNSATAITQSRDKLYSLQLLLNSGIDIPTTGFANSPLDTDNLIKMVGGSPLIVKLLEGTQGKGVVLAETK
KAAESVINAFKSLNANILVQEFIKEANGKDIRCFVIDGKVVAAIQREAMPGEFRANIHLGGTASVIKVTAEEKKIAIKAA
KAMDLKVAGVDIIRSSKGPLLLEVNSSPGLEGIEGATNKDVAGEMIKAIEKNFKL

Sequences:

>Translated_455_residues
MLQNKVILGSEEWCSFPELGIPTIKARVDSGAKTSAMHAINIAPFIKNDANWVKFDINPIQNNIKTIIHCEAPLVDKRIV
KSSSGYREHRYVIQTHLKIGDAKWPIEMTLTNRDSMGFRMLLGREAMSGRVLVDPEQKYLLGQPSPETLKELYQNSEKAT
TGLRIGLLASNPELYSNKRIMEAGEMRGHEMHFLNIKECYMKLDAKKPEIHYRGGKILNEFDAIIPRIRPSITFYGCALT
RQFEALKVFVLNSATAITQSRDKLYSLQLLLNSGIDIPTTGFANSPLDTDNLIKMVGGSPLIVKLLEGTQGKGVVLAETK
KAAESVINAFKSLNANILVQEFIKEANGKDIRCFVIDGKVVAAIQREAMPGEFRANIHLGGTASVIKVTAEEKKIAIKAA
KAMDLKVAGVDIIRSSKGPLLLEVNSSPGLEGIEGATNKDVAGEMIKAIEKNFKL
>Mature_455_residues
MLQNKVILGSEEWCSFPELGIPTIKARVDSGAKTSAMHAINIAPFIKNDANWVKFDINPIQNNIKTIIHCEAPLVDKRIV
KSSSGYREHRYVIQTHLKIGDAKWPIEMTLTNRDSMGFRMLLGREAMSGRVLVDPEQKYLLGQPSPETLKELYQNSEKAT
TGLRIGLLASNPELYSNKRIMEAGEMRGHEMHFLNIKECYMKLDAKKPEIHYRGGKILNEFDAIIPRIRPSITFYGCALT
RQFEALKVFVLNSATAITQSRDKLYSLQLLLNSGIDIPTTGFANSPLDTDNLIKMVGGSPLIVKLLEGTQGKGVVLAETK
KAAESVINAFKSLNANILVQEFIKEANGKDIRCFVIDGKVVAAIQREAMPGEFRANIHLGGTASVIKVTAEEKKIAIKAA
KAMDLKVAGVDIIRSSKGPLLLEVNSSPGLEGIEGATNKDVAGEMIKAIEKNFKL

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6 [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI164663810, Length=206, Percent_Identity=27.6699029126214, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI134254462, Length=209, Percent_Identity=28.2296650717703, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1787076, Length=290, Percent_Identity=57.9310344827586, Blast_Score=342, Evalue=2e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008503
- InterPro:   IPR009007
- InterPro:   IPR004666 [H]

Pfam domain/function: PF05618 DUF785; PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 50159; Mature: 50159

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQNKVILGSEEWCSFPELGIPTIKARVDSGAKTSAMHAINIAPFIKNDANWVKFDINPI
CCCCEEEECCHHHCCCCCCCCCEEEEECCCCCCCCEEEEEEECCEECCCCCEEEEECCHH
QNNIKTIIHCEAPLVDKRIVKSSSGYREHRYVIQTHLKIGDAKWPIEMTLTNRDSMGFRM
CCCCEEEEEECCCCHHHHHHHCCCCCCHHEEEEEEEEEECCCCCCEEEEEECCCCCCEEE
LLGREAMSGRVLVDPEQKYLLGQPSPETLKELYQNSEKATTGLRIGLLASNPELYSNKRI
EECCHHHCCEEEECCCHHEEECCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCHHCCCCH
MEAGEMRGHEMHFLNIKECYMKLDAKKPEIHYRGGKILNEFDAIIPRIRPSITFYGCALT
HHHHHCCCCEEEEEEHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCEEEEEEHHH
RQFEALKVFVLNSATAITQSRDKLYSLQLLLNSGIDIPTTGFANSPLDTDNLIKMVGGSP
HCCEEEEEEEECCCHHHHHCHHHHEEEEEHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCE
LIVKLLEGTQGKGVVLAETKKAAESVINAFKSLNANILVQEFIKEANGKDIRCFVIDGKV
EEEEEECCCCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEECCCE
VAAIQREAMPGEFRANIHLGGTASVIKVTAEEKKIAIKAAKAMDLKVAGVDIIRSSKGPL
EEEEHHCCCCCCEEEEEEECCCCEEEEEECCCCEEEEEEEEEEEEEEECEEEEECCCCCE
LLEVNSSPGLEGIEGATNKDVAGEMIKAIEKNFKL
EEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLQNKVILGSEEWCSFPELGIPTIKARVDSGAKTSAMHAINIAPFIKNDANWVKFDINPI
CCCCEEEECCHHHCCCCCCCCCEEEEECCCCCCCCEEEEEEECCEECCCCCEEEEECCHH
QNNIKTIIHCEAPLVDKRIVKSSSGYREHRYVIQTHLKIGDAKWPIEMTLTNRDSMGFRM
CCCCEEEEEECCCCHHHHHHHCCCCCCHHEEEEEEEEEECCCCCCEEEEEECCCCCCEEE
LLGREAMSGRVLVDPEQKYLLGQPSPETLKELYQNSEKATTGLRIGLLASNPELYSNKRI
EECCHHHCCEEEECCCHHEEECCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCHHCCCCH
MEAGEMRGHEMHFLNIKECYMKLDAKKPEIHYRGGKILNEFDAIIPRIRPSITFYGCALT
HHHHHCCCCEEEEEEHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCEEEEEEHHH
RQFEALKVFVLNSATAITQSRDKLYSLQLLLNSGIDIPTTGFANSPLDTDNLIKMVGGSP
HCCEEEEEEEECCCHHHHHCHHHHEEEEEHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCE
LIVKLLEGTQGKGVVLAETKKAAESVINAFKSLNANILVQEFIKEANGKDIRCFVIDGKV
EEEEEECCCCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEECCCE
VAAIQREAMPGEFRANIHLGGTASVIKVTAEEKKIAIKAAKAMDLKVAGVDIIRSSKGPL
EEEEHHCCCCCCEEEEEEECCCCEEEEEECCCCEEEEEEEEEEEEEEECEEEEECCCCCE
LLEVNSSPGLEGIEGATNKDVAGEMIKAIEKNFKL
EEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA