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The map label for this gene is arsB [H]

Identifier: 146284582

GI number: 146284582

Start: 85493

End: 86782

Strand: Direct

Name: arsB [H]

Synonym: Ent638_4256

Alternate gene names: 146284582

Gene position: 85493-86782 (Clockwise)

Preceding gene: 146284581

Following gene: 146284583

Centisome position: 54.2

GC content: 55.19

Gene sequence:

>1290_bases
ATGCTTTTAGCAGGGAGTATATTTTTACTGACGCTGGTACTGGTGATCTGGCAACCCAGAGGCCTGAGTATTGGCTGGAG
CGCGAGTATCGGGGCTGTGCTGGCTCTGGGAACTGGTGTCATCCATATCGCTGATATTCCCGTTGTCTGGAATATCGTCT
GGAACGCGACAGCGGCATTTATTGCGGTCATCATCATCAGCCTGCTGCTTGATGAGTCCGGCTTCTTTGAATGGGCCGCA
CTGCACGTTTCCCGCTGGGGTAACGGACGTGGCCGCCTTCTGTTTACCTGGATAGTGTTGCTCGGTGCCGCTGTTGCTGC
GTTGTTTGCCAATGACGGCGCCGCGCTGATTCTGACACCGATTGTGATTGCGATGCTGCTCGCTCTGGGGTTCAGCCAGG
GAACGACACTGGCCTTTGTCATGGCGGCAGGATTTATTGCGGATACGGCCAGCCTGCCGCTCATTGTTTCTAACCTGGTG
AATATTGTCTCAGCAGATTTCTTCGGACTGGGCTTTACGCAGTACGCCTCCGTTATGGTGCCCGTGGACGTGGCAGCTAT
TGCGGCTACGCTGGCCATGCTGCATATCTTCTTCCGCAGGGATATTCCGGCCACGTATGACGTTTCGCTGCTGAAGACGC
CTGCCAGTGCGATAAAGGATCCGGCGACCTTCAGGGCGGGCTGGATTGTCCTGTTATTACTGCTTGTCGGTTTCTTTGTT
CTGGAGCCCCTGGGGATCCCGGTCAGCGCGATAGCAGCAGCTGGCGCAGCTGTGCTTTTCGTGGTAGCGAAAAGAGGTCA
TGCCATCAACACCGGGAAAGTCCTGCGCGGTGCGCCATGGCAGATCGTTATTTTCTCGCTGGGTATGTACCTGGTGGTCT
ATGGCCTGCGCAATGCTGGCCTTACAGAGTACCTGTCAGGCGTGCTGAGCCTGCTGGCAGACAAAGGGTTATGGGCAGCG
ACGTTCGGTACCGGCTTCCTGACGGCATTTCTCTCATCAGTGATGAACAATATGCCGACCGTGCTGATTGGTGCGTTGTC
GATTGACGGGAGTACGGCGACTGGCGTCGTCAAAGAGGCAATGGTTTATGCCAATGTGATTGGCTGCGATTTAGGCCCGA
AAATCACCCCGATTGGCAGTCTGGCTACCCTGCTGTGGCTGCATGTGCTTTCCCAGAAAAATATGACGATCACATGGGGA
TATTACTTCCGTACCGGCATTGTTATGACTGTACCTGTGCTGTTTGTCACTCTGGCCGCGCTGGCGTGGCGGCTCTCTGT
CACTTTGTAA

Upstream 100 bases:

>100_bases
GTCTGCGAGAGGATGTGCGTGAATGGCTGCAAAAAGCAGCCTGCACCTCCTGCTGAGACAGGTAAACACATTTACTTAAT
CATATGTGATGGAGTCTGGA

Downstream 100 bases:

>100_bases
TGAGATACTGATATGAGCAACATTACCATCTATCACAACCCGGCCTGTGGTACTTCACGCAACACACTGGAGATGATCCG
CAACAGCGGCAATGAACCGA

Product: arsenical pump membrane protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: Arsenic efflux pump protein [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MLLAGSIFLLTLVLVIWQPRGLSIGWSASIGAVLALGTGVIHIADIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAA
LHVSRWGNGRGRLLFTWIVLLGAAVAALFANDGAALILTPIVIAMLLALGFSQGTTLAFVMAAGFIADTASLPLIVSNLV
NIVSADFFGLGFTQYASVMVPVDVAAIAATLAMLHIFFRRDIPATYDVSLLKTPASAIKDPATFRAGWIVLLLLLVGFFV
LEPLGIPVSAIAAAGAAVLFVVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLSLLADKGLWAA
TFGTGFLTAFLSSVMNNMPTVLIGALSIDGSTATGVVKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQKNMTITWG
YYFRTGIVMTVPVLFVTLAALAWRLSVTL

Sequences:

>Translated_429_residues
MLLAGSIFLLTLVLVIWQPRGLSIGWSASIGAVLALGTGVIHIADIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAA
LHVSRWGNGRGRLLFTWIVLLGAAVAALFANDGAALILTPIVIAMLLALGFSQGTTLAFVMAAGFIADTASLPLIVSNLV
NIVSADFFGLGFTQYASVMVPVDVAAIAATLAMLHIFFRRDIPATYDVSLLKTPASAIKDPATFRAGWIVLLLLLVGFFV
LEPLGIPVSAIAAAGAAVLFVVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLSLLADKGLWAA
TFGTGFLTAFLSSVMNNMPTVLIGALSIDGSTATGVVKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQKNMTITWG
YYFRTGIVMTVPVLFVTLAALAWRLSVTL
>Mature_429_residues
MLLAGSIFLLTLVLVIWQPRGLSIGWSASIGAVLALGTGVIHIADIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAA
LHVSRWGNGRGRLLFTWIVLLGAAVAALFANDGAALILTPIVIAMLLALGFSQGTTLAFVMAAGFIADTASLPLIVSNLV
NIVSADFFGLGFTQYASVMVPVDVAAIAATLAMLHIFFRRDIPATYDVSLLKTPASAIKDPATFRAGWIVLLLLLVGFFV
LEPLGIPVSAIAAAGAAVLFVVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLSLLADKGLWAA
TFGTGFLTAFLSSVMNNMPTVLIGALSIDGSTATGVVKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQKNMTITWG
YYFRTGIVMTVPVLFVTLAALAWRLSVTL

Specific function: Involved in arsenical resistance. Thought to form the channel of an arsenite pump [H]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=429, Percent_Identity=89.5104895104895, Blast_Score=736, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 45320; Mature: 45320

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS00626 RCC1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLAGSIFLLTLVLVIWQPRGLSIGWSASIGAVLALGTGVIHIADIPVVWNIVWNATAAF
CCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHH
IAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLGAAVAALFANDGAALILTP
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IVIAMLLALGFSQGTTLAFVMAAGFIADTASLPLIVSNLVNIVSADFFGLGFTQYASVMV
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
PVDVAAIAATLAMLHIFFRRDIPATYDVSLLKTPASAIKDPATFRAGWIVLLLLLVGFFV
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHH
LEPLGIPVSAIAAAGAAVLFVVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLRNAG
HCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
LTEYLSGVLSLLADKGLWAATFGTGFLTAFLSSVMNNMPTVLIGALSIDGSTATGVVKEA
HHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHH
MVYANVIGCDLGPKITPIGSLATLLWLHVLSQKNMTITWGYYFRTGIVMTVPVLFVTLAA
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
LAWRLSVTL
HHHHHHCCC
>Mature Secondary Structure
MLLAGSIFLLTLVLVIWQPRGLSIGWSASIGAVLALGTGVIHIADIPVVWNIVWNATAAF
CCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHH
IAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLGAAVAALFANDGAALILTP
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IVIAMLLALGFSQGTTLAFVMAAGFIADTASLPLIVSNLVNIVSADFFGLGFTQYASVMV
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
PVDVAAIAATLAMLHIFFRRDIPATYDVSLLKTPASAIKDPATFRAGWIVLLLLLVGFFV
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHH
LEPLGIPVSAIAAAGAAVLFVVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLRNAG
HCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
LTEYLSGVLSLLADKGLWAATFGTGFLTAFLSSVMNNMPTVLIGALSIDGSTATGVVKEA
HHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHH
MVYANVIGCDLGPKITPIGSLATLLWLHVLSQKNMTITWGYYFRTGIVMTVPVLFVTLAA
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
LAWRLSVTL
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9006011 [H]