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The map label for this gene is rph

Identifier: 138896231

GI number: 138896231

Start: 2703230

End: 2703997

Strand: Reverse

Name: rph

Synonym: GTNG_2594

Alternate gene names: 138896231

Gene position: 2703997-2703230 (Counterclockwise)

Preceding gene: 138896232

Following gene: 138896230

Centisome position: 76.16

GC content: 55.6

Gene sequence:

>768_bases
ATGAGAACGGACGGACGAAATAACCGCGAGCTGCGCCCTGTACATATACAACCGCATTATATCAAGCATGCTGAAGGCTC
GGTGCTGATTGAAATCGGCGACACGAGAGTGATTTGCACGGCGACGGTTGAAGACAAAGTACCGCCGTTTATGCGCGGTG
GCGGGAAAGGATGGATTACGGCCGAATATGGGATGTTGCCAAGAGCGACAGAACAACGGAATGCGAGGGAAGCAAGCAAA
GGGAAAGTGTCGGGACGCACGATGGAAATTCAGCGTCTGATCGGCCGGGCGCTCCGTTCGGTCGTTGAGTTGGAACAGCT
CGGCGAGCGGACCGTCTGGATTGACTGTGACGTCATTCAGGCGGACGGTGGAACACGAACGGCGTCGATCACCGGTGCGT
ATGTGGCGCTCGTGTTGGCGCTCTCCAAACTGGTTGCAGAAGGGAAGCTTGAGTCGCTCCCGGTTCGCGATTTTCTCGCT
GCCACGTCAGTCGGCATCGATCCTGAACACGGGGTTATTCTCGATTTAAATTACAGTGAGGACGCACGGGCGAAAGTCGA
CATGAACGTCGTCATGACCGGAGCCGGCCAGTTTGTCGAAATTCAAGGCACCGGGGAAGAAGCGTCTTTTTCGCGCGCTG
AGCTGGAGGAATTACTCGAGACGGCCCAACTAGGTATTGAACAGCTCATCACCATTCAGCGCCGTGTATTGGGGGAGCAG
GCAGCCCTCATCGGAGAGAAAGTAGAACAGGAGGGGAAATCAGAGTGA

Upstream 100 bases:

>100_bases
AGGAACGATGTTTGGGACCACTTGGGATTCGCTTGAAGGAGCAGGCTGACACTTGGCTGTCAAGCGGAAGCCATCCAAGG
AGGAAAAAGGAGGACTGTCG

Downstream 100 bases:

>100_bases
AGGAAATTGTCATTGCCACGAAAAACGCAGGCAAGGTGCGCGAGTTTGCCGCCATGTTTGCCAAGCGAGGCATCGAAGTG
AAATCGCTGCTCGATTTTCC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MRTDGRNNRELRPVHIQPHYIKHAEGSVLIEIGDTRVICTATVEDKVPPFMRGGGKGWITAEYGMLPRATEQRNAREASK
GKVSGRTMEIQRLIGRALRSVVELEQLGERTVWIDCDVIQADGGTRTASITGAYVALVLALSKLVAEGKLESLPVRDFLA
ATSVGIDPEHGVILDLNYSEDARAKVDMNVVMTGAGQFVEIQGTGEEASFSRAELEELLETAQLGIEQLITIQRRVLGEQ
AALIGEKVEQEGKSE

Sequences:

>Translated_255_residues
MRTDGRNNRELRPVHIQPHYIKHAEGSVLIEIGDTRVICTATVEDKVPPFMRGGGKGWITAEYGMLPRATEQRNAREASK
GKVSGRTMEIQRLIGRALRSVVELEQLGERTVWIDCDVIQADGGTRTASITGAYVALVLALSKLVAEGKLESLPVRDFLA
ATSVGIDPEHGVILDLNYSEDARAKVDMNVVMTGAGQFVEIQGTGEEASFSRAELEELLETAQLGIEQLITIQRRVLGEQ
AALIGEKVEQEGKSE
>Mature_255_residues
MRTDGRNNRELRPVHIQPHYIKHAEGSVLIEIGDTRVICTATVEDKVPPFMRGGGKGWITAEYGMLPRATEQRNAREASK
GKVSGRTMEIQRLIGRALRSVVELEQLGERTVWIDCDVIQADGGTRTASITGAYVALVLALSKLVAEGKLESLPVRDFLA
ATSVGIDPEHGVILDLNYSEDARAKVDMNVVMTGAGQFVEIQGTGEEASFSRAELEELLETAQLGIEQLITIQRRVLGEQ
AALIGEKVEQEGKSE

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=62.085308056872, Blast_Score=264, Evalue=4e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_GEOTN (A4IRI5)

Other databases:

- EMBL:   CP000557
- RefSeq:   YP_001126684.1
- ProteinModelPortal:   A4IRI5
- SMR:   A4IRI5
- STRING:   A4IRI5
- GeneID:   4967520
- GenomeReviews:   CP000557_GR
- KEGG:   gtn:GTNG_2594
- NMPDR:   fig|420246.5.peg.2500
- eggNOG:   COG0689
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- PhylomeDB:   A4IRI5
- ProtClustDB:   PRK00173
- BioCyc:   GTHE420246:GTNG_2594-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 27850; Mature: 27850

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTDGRNNRELRPVHIQPHYIKHAEGSVLIEIGDTRVICTATVEDKVPPFMRGGGKGWIT
CCCCCCCCCCEEEEECCCCEEEECCCEEEEEECCCEEEEEEECCCCCCHHHHCCCCEEEE
AEYGMLPRATEQRNAREASKGKVSGRTMEIQRLIGRALRSVVELEQLGERTVWIDCDVIQ
EECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEE
ADGGTRTASITGAYVALVLALSKLVAEGKLESLPVRDFLAATSVGIDPEHGVILDLNYSE
CCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCC
DARAKVDMNVVMTGAGQFVEIQGTGEEASFSRAELEELLETAQLGIEQLITIQRRVLGEQ
CCCEEEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AALIGEKVEQEGKSE
HHHHHHHHHHCCCCC
>Mature Secondary Structure
MRTDGRNNRELRPVHIQPHYIKHAEGSVLIEIGDTRVICTATVEDKVPPFMRGGGKGWIT
CCCCCCCCCCEEEEECCCCEEEECCCEEEEEECCCEEEEEEECCCCCCHHHHCCCCEEEE
AEYGMLPRATEQRNAREASKGKVSGRTMEIQRLIGRALRSVVELEQLGERTVWIDCDVIQ
EECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEE
ADGGTRTASITGAYVALVLALSKLVAEGKLESLPVRDFLAATSVGIDPEHGVILDLNYSE
CCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCC
DARAKVDMNVVMTGAGQFVEIQGTGEEASFSRAELEELLETAQLGIEQLITIQRRVLGEQ
CCCEEEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AALIGEKVEQEGKSE
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA