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The map label for this gene is leuC [H]

Identifier: 138896222

GI number: 138896222

Start: 2693179

End: 2694621

Strand: Reverse

Name: leuC [H]

Synonym: GTNG_2585

Alternate gene names: 138896222

Gene position: 2694621-2693179 (Counterclockwise)

Preceding gene: 138896223

Following gene: 229577455

Centisome position: 75.9

GC content: 54.47

Gene sequence:

>1443_bases
ATGCATGAGACGAGCGAGGTGAAGGCAATGAAACCAAAAACGATCATCGAGAAAATATGGGAAAACCATGTCGTTTATCG
AGAAGAAGGAAAGCCGGATTTGCTGTATATCGATTTGCATTTAGTGCATGAAGTGACATCGCCGCAGGCATTTGAAGGGC
TGCGCCAAAACGGGCGCAAAGTTCGGCGTCCAGATTTGACGTTTGCGACGATGGACCATAACGTGCCGACTGTCAACCGG
TTTGTCATTACTGATGAAGTGGCACGCAACCAAATCGCTGCGCTTGAGCGCAACTGCCGCGAGTTTGGCATCCCGCTTGC
TGACTTGCACAGCGAAGAACAAGGAATCGTTCATGTGATTGGTCCGGAACTTGGCTTGACTCAGCCAGGAAAAACGATCG
TCTGCGGCGACAGTCATACATCGACGCACGGAGCGTTTGGCGCCTTGGCGTTTGGCATCGGAACAAGTGAAGTCGAGCAC
GTATTGGCGACACAAACGCTTTGGCAGCATAAACCGAAAACGTTGCAAATCCGCATCAACGGCCAGCTCGGCAAAGGAGT
GACGGCGAAAGACGTCATTTTGGCTATCATTGGCCGCTACGGCGTCGGCGTCGGCACCGGTTATATTATCGAGTTTACCG
GTGAGGCGATCCGTCGGATGTCGATGGAAGAGCGGATGACGATTTGCAACATGTCGATCGAAGCCGGGGCGCGCGCCGGC
CTCATCAGTCCGGATGAAACGACATTTGCCTATTTGCGAGGACGCAAATATGCACCAAAAGGAGAGGAGTTTGACCAAGC
GGTTGAACGGTGGCGTGCGCTTGCCAGCGACGAAGGGGCCGAGTATGACAAAACGATCGAAATTGACGCCTCAACCATTG
CCCCGATGGTAACGTGGGGCACGAACCCATCGATGAGCACATCGGTTGATGGAACGGTGCCCGACCCGGAACAGTTTGAA
AGCGAAACAGAGCGAAACGCGGTGCGCCGGGCGCTTGAGTATATGGGGCTGAAGCCGGGCATGTCGATTACCGAAATTCC
GGTGCAGCATGTATTTATCGGTTCCTGTACGAACTCACGCATCAGCGATTTGCGCGAAGCGGCGAAAATCGTCAAGGGCC
AAAAAGTAGCTCCTGGTGTCAGAGCGCTTGTTGTACCCGGGTCGCAGCAAGTAAAAAAACAGGCTGAAGAAGAAGGACTC
GCGCAAATTTTCCTTGATGCCGGCTTTGAATGGCGCGATGCTGGCTGCAGCGCCTGTTTGGGCATGAACCCGGACATCAT
TCCGGAAGGCGAGCATTGTGCGTCGACGTCGAACCGCAACTTTGAAGGGCGTCAAGGAAAAGGAGCGCGCACCCATCTTG
TCAGCCCGGCGATGGCTGCTGCCGCTGCCATTTACGGCCGCTTTGTCGATGTGCGTCAGCTCGAAGCTGAACCGGTCCGG
TAA

Upstream 100 bases:

>100_bases
GCGCAAAGCAACGGTCGTGTCGTATCGACAGATGAAATGATCGAGGAAATCAAAACAGCTGTACTGGACTATACAGCGAT
CGCGCAAATCATGACCGTTT

Downstream 100 bases:

>100_bases
GGGATGCTTCTTGAATCCGCAGCATCCCACCTTAGGGCCATCCGCTTTTGGAACGGTGACTGATACATCTGATGGAAGGG
GAGAGACGATGAAGCCGTTT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MHETSEVKAMKPKTIIEKIWENHVVYREEGKPDLLYIDLHLVHEVTSPQAFEGLRQNGRKVRRPDLTFATMDHNVPTVNR
FVITDEVARNQIAALERNCREFGIPLADLHSEEQGIVHVIGPELGLTQPGKTIVCGDSHTSTHGAFGALAFGIGTSEVEH
VLATQTLWQHKPKTLQIRINGQLGKGVTAKDVILAIIGRYGVGVGTGYIIEFTGEAIRRMSMEERMTICNMSIEAGARAG
LISPDETTFAYLRGRKYAPKGEEFDQAVERWRALASDEGAEYDKTIEIDASTIAPMVTWGTNPSMSTSVDGTVPDPEQFE
SETERNAVRRALEYMGLKPGMSITEIPVQHVFIGSCTNSRISDLREAAKIVKGQKVAPGVRALVVPGSQQVKKQAEEEGL
AQIFLDAGFEWRDAGCSACLGMNPDIIPEGEHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIYGRFVDVRQLEAEPVR

Sequences:

>Translated_480_residues
MHETSEVKAMKPKTIIEKIWENHVVYREEGKPDLLYIDLHLVHEVTSPQAFEGLRQNGRKVRRPDLTFATMDHNVPTVNR
FVITDEVARNQIAALERNCREFGIPLADLHSEEQGIVHVIGPELGLTQPGKTIVCGDSHTSTHGAFGALAFGIGTSEVEH
VLATQTLWQHKPKTLQIRINGQLGKGVTAKDVILAIIGRYGVGVGTGYIIEFTGEAIRRMSMEERMTICNMSIEAGARAG
LISPDETTFAYLRGRKYAPKGEEFDQAVERWRALASDEGAEYDKTIEIDASTIAPMVTWGTNPSMSTSVDGTVPDPEQFE
SETERNAVRRALEYMGLKPGMSITEIPVQHVFIGSCTNSRISDLREAAKIVKGQKVAPGVRALVVPGSQQVKKQAEEEGL
AQIFLDAGFEWRDAGCSACLGMNPDIIPEGEHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIYGRFVDVRQLEAEPVR
>Mature_480_residues
MHETSEVKAMKPKTIIEKIWENHVVYREEGKPDLLYIDLHLVHEVTSPQAFEGLRQNGRKVRRPDLTFATMDHNVPTVNR
FVITDEVARNQIAALERNCREFGIPLADLHSEEQGIVHVIGPELGLTQPGKTIVCGDSHTSTHGAFGALAFGIGTSEVEH
VLATQTLWQHKPKTLQIRINGQLGKGVTAKDVILAIIGRYGVGVGTGYIIEFTGEAIRRMSMEERMTICNMSIEAGARAG
LISPDETTFAYLRGRKYAPKGEEFDQAVERWRALASDEGAEYDKTIEIDASTIAPMVTWGTNPSMSTSVDGTVPDPEQFE
SETERNAVRRALEYMGLKPGMSITEIPVQHVFIGSCTNSRISDLREAAKIVKGQKVAPGVRALVVPGSQQVKKQAEEEGL
AQIFLDAGFEWRDAGCSACLGMNPDIIPEGEHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIYGRFVDVRQLEAEPVR

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=367, Percent_Identity=30.5177111716621, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI8659555, Length=441, Percent_Identity=27.2108843537415, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI41352693, Length=370, Percent_Identity=27.027027027027, Blast_Score=106, Evalue=6e-23,
Organism=Escherichia coli, GI1786259, Length=464, Percent_Identity=62.7155172413793, Blast_Score=598, Evalue=1e-172,
Organism=Escherichia coli, GI1787531, Length=376, Percent_Identity=27.1276595744681, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI87081781, Length=344, Percent_Identity=27.906976744186, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI2367097, Length=344, Percent_Identity=25.5813953488372, Blast_Score=69, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI25149337, Length=366, Percent_Identity=32.7868852459016, Blast_Score=144, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI32564738, Length=376, Percent_Identity=32.7127659574468, Blast_Score=144, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI25149342, Length=313, Percent_Identity=32.5878594249201, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17568399, Length=462, Percent_Identity=25.974025974026, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321429, Length=472, Percent_Identity=58.8983050847458, Blast_Score=561, Evalue=1e-160,
Organism=Saccharomyces cerevisiae, GI6320440, Length=468, Percent_Identity=28.8461538461538, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6322261, Length=393, Percent_Identity=31.2977099236641, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6323335, Length=361, Percent_Identity=30.1939058171745, Blast_Score=140, Evalue=5e-34,
Organism=Drosophila melanogaster, GI28571643, Length=363, Percent_Identity=30.3030303030303, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI281365315, Length=386, Percent_Identity=28.7564766839378, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI17864292, Length=386, Percent_Identity=28.7564766839378, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI161076999, Length=386, Percent_Identity=28.7564766839378, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI17137564, Length=382, Percent_Identity=28.0104712041885, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24645686, Length=478, Percent_Identity=26.3598326359833, Blast_Score=100, Evalue=3e-21,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 52643; Mature: 52643

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHETSEVKAMKPKTIIEKIWENHVVYREEGKPDLLYIDLHLVHEVTSPQAFEGLRQNGRK
CCCCCCCCCCCHHHHHHHHHHCCEEEECCCCCCEEEEEEEEEHHCCCCHHHHHHHHCCCC
VRRPDLTFATMDHNVPTVNRFVITDEVARNQIAALERNCREFGIPLADLHSEEQGIVHVI
CCCCCCEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEE
GPELGLTQPGKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLWQHKPKTLQIRIN
CCCCCCCCCCCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEC
GQLGKGVTAKDVILAIIGRYGVGVGTGYIIEFTGEAIRRMSMEERMTICNMSIEAGARAG
CCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCHHCCCCCC
LISPDETTFAYLRGRKYAPKGEEFDQAVERWRALASDEGAEYDKTIEIDASTIAPMVTWG
CCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECHHHCCCEEEEC
TNPSMSTSVDGTVPDPEQFESETERNAVRRALEYMGLKPGMSITEIPVQHVFIGSCTNSR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEEEEECCCCHH
ISDLREAAKIVKGQKVAPGVRALVVPGSQQVKKQAEEEGLAQIFLDAGFEWRDAGCSACL
HHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEC
GMNPDIIPEGEHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIYGRFVDVRQLEAEPVR
CCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MHETSEVKAMKPKTIIEKIWENHVVYREEGKPDLLYIDLHLVHEVTSPQAFEGLRQNGRK
CCCCCCCCCCCHHHHHHHHHHCCEEEECCCCCCEEEEEEEEEHHCCCCHHHHHHHHCCCC
VRRPDLTFATMDHNVPTVNRFVITDEVARNQIAALERNCREFGIPLADLHSEEQGIVHVI
CCCCCCEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEE
GPELGLTQPGKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLWQHKPKTLQIRIN
CCCCCCCCCCCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEC
GQLGKGVTAKDVILAIIGRYGVGVGTGYIIEFTGEAIRRMSMEERMTICNMSIEAGARAG
CCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCHHCCCCCC
LISPDETTFAYLRGRKYAPKGEEFDQAVERWRALASDEGAEYDKTIEIDASTIAPMVTWG
CCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECHHHCCCEEEEC
TNPSMSTSVDGTVPDPEQFESETERNAVRRALEYMGLKPGMSITEIPVQHVFIGSCTNSR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEEEEECCCCHH
ISDLREAAKIVKGQKVAPGVRALVVPGSQQVKKQAEEEGLAQIFLDAGFEWRDAGCSACL
HHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEC
GMNPDIIPEGEHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIYGRFVDVRQLEAEPVR
CCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA