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The map label for this gene is leuB [H]

Identifier: 138896223

GI number: 138896223

Start: 2694614

End: 2695729

Strand: Reverse

Name: leuB [H]

Synonym: GTNG_2586

Alternate gene names: 138896223

Gene position: 2695729-2694614 (Counterclockwise)

Preceding gene: 138896224

Following gene: 138896222

Centisome position: 75.93

GC content: 53.76

Gene sequence:

>1116_bases
ATGGGAAACTATCGGATTGCCGTCTTGCCGGGAGATGGTATCGGCAAAGAAGTGACGTCTGGAGCGGTGGAAGTATTAAA
AGCGGTCGGAATCCGGTTTGGCCACGAGTTTACATTCGAATACGGGTTGATTGGCGGAGCGGCGATCGATGAAGTAGGGA
CGCCGCTTCCAGAAGAAACGCTTCGCCTTTGCCAACAAAGCGATGCTGTCTTGCTTGGGGCGGTCGGCGGTCCGAAGTGG
GACCAAAATCCTCCGCACTTGCGCCCGGAAAAAGGGTTGCTTGCTATCCGTCAACAGCTTGGGCTGTACGCGAACTTGCG
GCCGGTCGTCTGCTATGACAGTTTGGATTCCGCTTCGCCGTTAAAATCCGATCTCGTGCAAGGGGTAGATTTTATCATCG
TTCGTGAATTGACCGGCGGCATTTACTTCGGCCAACCGAGCGGCCGCGTTGTTGAAAATGGGGAAGAAAAAGCCGTTGAC
ACACTCTTATATAAAAAGGAAGAGATTGAACGGATTGTGCGCATGGCGTTTACGCTGGCACGTGGGCGGAAGAAAAAAGT
GACATCGGTCGATAAGGCGAACGTGCTCTCCTCAAGCCGTTTATGGCGGGAAGTGGCCGAAGAGGTGGCTAAACAATTCC
CGGATGTAGAGCTTGAACATATGCTGGTCGACAATGCAGCGATGCAACTGATCCGCGCGCCTAAGCAGTTTGACGTGATC
GTCACTGAAAATATGTTTGGCGACATTTTAAGCGATGAAGCATCGATGTTGTCCGGCTCGCTCGGGATGTTGCCATCAGC
TAGCTTGTCGGCTTCCGGACCGAGCTTGTATGAGCCGGTGCACGGTTCGGCGCCCGATATCGCTGGCATGCGAAAGGCGA
ATCCGATTGCTGCTATTTTGTCGGCTGCCATGATGCTTCGCCTGTCGTTTGGGCTCACCGCCGAGGCGGAAGCGATCGAG
CATGCGGTGCAGCAGGCGCTCACTGCGGGCTTGCGAACGGCTGATCTAGCGCAAAGCAACGGTCGTGTCGTATCGACAGA
TGAAATGATCGAGGAAATCAAAACAGCTGTACTGGACTATACAGCGATCGCGCAAATCATGACCGTTTATGCATGA

Upstream 100 bases:

>100_bases
GCTAAAGCGTACATTAACGCGATGAACCGCATGTTTATGATCGAAGCGATGCGTGCTGAAAACGAAAAAGTGACAACACC
ATAAAACGGGGGGATCGAGC

Downstream 100 bases:

>100_bases
GACGAGCGAGGTGAAGGCAATGAAACCAAAAACGATCATCGAGAAAATATGGGAAAACCATGTCGTTTATCGAGAAGAAG
GAAAGCCGGATTTGCTGTAT

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MGNYRIAVLPGDGIGKEVTSGAVEVLKAVGIRFGHEFTFEYGLIGGAAIDEVGTPLPEETLRLCQQSDAVLLGAVGGPKW
DQNPPHLRPEKGLLAIRQQLGLYANLRPVVCYDSLDSASPLKSDLVQGVDFIIVRELTGGIYFGQPSGRVVENGEEKAVD
TLLYKKEEIERIVRMAFTLARGRKKKVTSVDKANVLSSSRLWREVAEEVAKQFPDVELEHMLVDNAAMQLIRAPKQFDVI
VTENMFGDILSDEASMLSGSLGMLPSASLSASGPSLYEPVHGSAPDIAGMRKANPIAAILSAAMMLRLSFGLTAEAEAIE
HAVQQALTAGLRTADLAQSNGRVVSTDEMIEEIKTAVLDYTAIAQIMTVYA

Sequences:

>Translated_371_residues
MGNYRIAVLPGDGIGKEVTSGAVEVLKAVGIRFGHEFTFEYGLIGGAAIDEVGTPLPEETLRLCQQSDAVLLGAVGGPKW
DQNPPHLRPEKGLLAIRQQLGLYANLRPVVCYDSLDSASPLKSDLVQGVDFIIVRELTGGIYFGQPSGRVVENGEEKAVD
TLLYKKEEIERIVRMAFTLARGRKKKVTSVDKANVLSSSRLWREVAEEVAKQFPDVELEHMLVDNAAMQLIRAPKQFDVI
VTENMFGDILSDEASMLSGSLGMLPSASLSASGPSLYEPVHGSAPDIAGMRKANPIAAILSAAMMLRLSFGLTAEAEAIE
HAVQQALTAGLRTADLAQSNGRVVSTDEMIEEIKTAVLDYTAIAQIMTVYA
>Mature_370_residues
GNYRIAVLPGDGIGKEVTSGAVEVLKAVGIRFGHEFTFEYGLIGGAAIDEVGTPLPEETLRLCQQSDAVLLGAVGGPKWD
QNPPHLRPEKGLLAIRQQLGLYANLRPVVCYDSLDSASPLKSDLVQGVDFIIVRELTGGIYFGQPSGRVVENGEEKAVDT
LLYKKEEIERIVRMAFTLARGRKKKVTSVDKANVLSSSRLWREVAEEVAKQFPDVELEHMLVDNAAMQLIRAPKQFDVIV
TENMFGDILSDEASMLSGSLGMLPSASLSASGPSLYEPVHGSAPDIAGMRKANPIAAILSAAMMLRLSFGLTAEAEAIEH
AVQQALTAGLRTADLAQSNGRVVSTDEMIEEIKTAVLDYTAIAQIMTVYA

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=354, Percent_Identity=31.9209039548023, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI28178816, Length=320, Percent_Identity=30.9375, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI28178821, Length=320, Percent_Identity=30.9375, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI4758582, Length=360, Percent_Identity=28.8888888888889, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI28178838, Length=328, Percent_Identity=29.8780487804878, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI28178819, Length=140, Percent_Identity=37.8571428571429, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI87081683, Length=349, Percent_Identity=57.0200573065903, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1788101, Length=347, Percent_Identity=36.8876080691643, Blast_Score=198, Evalue=4e-52,
Organism=Escherichia coli, GI1787381, Length=393, Percent_Identity=27.735368956743, Blast_Score=91, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI71986051, Length=355, Percent_Identity=31.2676056338028, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI25144293, Length=370, Percent_Identity=30.8108108108108, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17550882, Length=358, Percent_Identity=31.0055865921788, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17505779, Length=362, Percent_Identity=28.7292817679558, Blast_Score=124, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6319830, Length=361, Percent_Identity=51.5235457063712, Blast_Score=340, Evalue=3e-94,
Organism=Saccharomyces cerevisiae, GI6322097, Length=363, Percent_Identity=35.5371900826446, Blast_Score=170, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6324709, Length=365, Percent_Identity=32.0547945205479, Blast_Score=152, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6324291, Length=371, Percent_Identity=29.9191374663073, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24643268, Length=347, Percent_Identity=33.1412103746398, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24643270, Length=347, Percent_Identity=33.1412103746398, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24661184, Length=362, Percent_Identity=29.0055248618785, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI281362242, Length=334, Percent_Identity=31.437125748503, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24648872, Length=334, Percent_Identity=31.437125748503, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI161078637, Length=357, Percent_Identity=28.5714285714286, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI161078635, Length=357, Percent_Identity=28.5714285714286, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI161078633, Length=357, Percent_Identity=28.5714285714286, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24650122, Length=357, Percent_Identity=28.5714285714286, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI161078639, Length=264, Percent_Identity=32.1969696969697, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI20130355, Length=336, Percent_Identity=23.8095238095238, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39941; Mature: 39810

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNYRIAVLPGDGIGKEVTSGAVEVLKAVGIRFGHEFTFEYGLIGGAAIDEVGTPLPEET
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCHHH
LRLCQQSDAVLLGAVGGPKWDQNPPHLRPEKGLLAIRQQLGLYANLRPVVCYDSLDSASP
HHHHHCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCH
LKSDLVQGVDFIIVRELTGGIYFGQPSGRVVENGEEKAVDTLLYKKEEIERIVRMAFTLA
HHHHHHCCHHHEEEEEHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RGRKKKVTSVDKANVLSSSRLWREVAEEVAKQFPDVELEHMLVDNAAMQLIRAPKQFDVI
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHEEE
VTENMFGDILSDEASMLSGSLGMLPSASLSASGPSLYEPVHGSAPDIAGMRKANPIAAIL
EECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
SAAMMLRLSFGLTAEAEAIEHAVQQALTAGLRTADLAQSNGRVVSTDEMIEEIKTAVLDY
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEHHHHHHHHHHHHHHH
TAIAQIMTVYA
HHHHHHHHHCC
>Mature Secondary Structure 
GNYRIAVLPGDGIGKEVTSGAVEVLKAVGIRFGHEFTFEYGLIGGAAIDEVGTPLPEET
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCHHH
LRLCQQSDAVLLGAVGGPKWDQNPPHLRPEKGLLAIRQQLGLYANLRPVVCYDSLDSASP
HHHHHCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCH
LKSDLVQGVDFIIVRELTGGIYFGQPSGRVVENGEEKAVDTLLYKKEEIERIVRMAFTLA
HHHHHHCCHHHEEEEEHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RGRKKKVTSVDKANVLSSSRLWREVAEEVAKQFPDVELEHMLVDNAAMQLIRAPKQFDVI
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHEEE
VTENMFGDILSDEASMLSGSLGMLPSASLSASGPSLYEPVHGSAPDIAGMRKANPIAAIL
EECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
SAAMMLRLSFGLTAEAEAIEHAVQQALTAGLRTADLAQSNGRVVSTDEMIEEIKTAVLDY
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEHHHHHHHHHHHHHHH
TAIAQIMTVYA
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA