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The map label for this gene is leuD [H]

Identifier: 229577455

GI number: 229577455

Start: 2692497

End: 2693090

Strand: Reverse

Name: leuD [H]

Synonym: GTNG_2584

Alternate gene names: 229577455

Gene position: 2693090-2692497 (Counterclockwise)

Preceding gene: 138896222

Following gene: 138896220

Centisome position: 75.85

GC content: 48.32

Gene sequence:

>594_bases
ATGAAGCCGTTTACGATTCACCGCGGAAAAACTGCTGGCATCGACCGGGTGAACATCGATACCGACCAAATCATTCCGAA
ACAGTTTTTAAAACGGATCGAGCGTACCGGATTTGGCCAATTTCTGTTTTACGACTGGCGCTATTTAAGCGATGGTACGC
CGAATCCGGAGTTTGAGCTCAACCGTCCGGAAAATGAAGGGGCAACGATTTTGGTCGCCGGTGACAATTTTGGCTGTGGT
TCATCGCGCGAACATGCCCCGTGGGCGCTTCAGGATTATGGATTTCGTGCGATCATTGCTCCTTCATTTGCCGATATCTT
TTACAATAACTGCTTAAAAAACGGACTGCTGCCCATTCGGCTTGATCAGGAAGATGTCCGCTATTTATTACAACAGAGCG
AGCGTGCTGACTACGAGCTGACCGTTTCGCTTGAAGAACAGCGGGTGTTTGATGACGAAGGGTTTTCACGCCCGTTTGAC
ATCGACCCGTACCGGAAGAAGTTGCTCTTAAAAGGCTGGGACGAAATTGATTTAACGTTTGTATATGAATCGCATATTGC
TGCCTACGAGCAAAAACATTGTCCGCGGCCGTAA

Upstream 100 bases:

>100_bases
CCGGTCCGGTAAGGGATGCTTCTTGAATCCGCAGCATCCCACCTTAGGGCCATCCGCTTTTGGAACGGTGACTGATACAT
CTGATGGAAGGGGAGAGACG

Downstream 100 bases:

>100_bases
GCGCTTGGCGTTCGGAACGGCAATGTAAGGCGAACTGAGGGTGTCCTAAAAGAAAAGGGACACCCTTTTTCATCACTGTA
TACATGCGCTGAATCGTTCT

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MKPFTIHRGKTAGIDRVNIDTDQIIPKQFLKRIERTGFGQFLFYDWRYLSDGTPNPEFELNRPENEGATILVAGDNFGCG
SSREHAPWALQDYGFRAIIAPSFADIFYNNCLKNGLLPIRLDQEDVRYLLQQSERADYELTVSLEEQRVFDDEGFSRPFD
IDPYRKKLLLKGWDEIDLTFVYESHIAAYEQKHCPRP

Sequences:

>Translated_197_residues
MKPFTIHRGKTAGIDRVNIDTDQIIPKQFLKRIERTGFGQFLFYDWRYLSDGTPNPEFELNRPENEGATILVAGDNFGCG
SSREHAPWALQDYGFRAIIAPSFADIFYNNCLKNGLLPIRLDQEDVRYLLQQSERADYELTVSLEEQRVFDDEGFSRPFD
IDPYRKKLLLKGWDEIDLTFVYESHIAAYEQKHCPRP
>Mature_197_residues
MKPFTIHRGKTAGIDRVNIDTDQIIPKQFLKRIERTGFGQFLFYDWRYLSDGTPNPEFELNRPENEGATILVAGDNFGCG
SSREHAPWALQDYGFRAIIAPSFADIFYNNCLKNGLLPIRLDQEDVRYLLQQSERADYELTVSLEEQRVFDDEGFSRPFD
IDPYRKKLLLKGWDEIDLTFVYESHIAAYEQKHCPRP

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=193, Percent_Identity=50.259067357513, Blast_Score=188, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6321429, Length=196, Percent_Identity=49.4897959183673, Blast_Score=189, Evalue=3e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 22961; Mature: 22961

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPFTIHRGKTAGIDRVNIDTDQIIPKQFLKRIERTGFGQFLFYDWRYLSDGTPNPEFEL
CCCEEECCCCCCCCEEEECCHHHCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCEEE
NRPENEGATILVAGDNFGCGSSREHAPWALQDYGFRAIIAPSFADIFYNNCLKNGLLPIR
CCCCCCCCEEEEECCCCCCCCCCCCCCEEHHHCCEEEEECCHHHHHHHHHHHHCCCEEEE
LDQEDVRYLLQQSERADYELTVSLEEQRVFDDEGFSRPFDIDPYRKKLLLKGWDEIDLTF
ECHHHHHHHHHHCCCCCEEEEEEECHHEECCCCCCCCCCCCCHHHHHHHHCCCCCEEEEE
VYESHIAAYEQKHCPRP
EEHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKPFTIHRGKTAGIDRVNIDTDQIIPKQFLKRIERTGFGQFLFYDWRYLSDGTPNPEFEL
CCCEEECCCCCCCCEEEECCHHHCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCEEE
NRPENEGATILVAGDNFGCGSSREHAPWALQDYGFRAIIAPSFADIFYNNCLKNGLLPIR
CCCCCCCCEEEEECCCCCCCCCCCCCCEEHHHCCEEEEECCHHHHHHHHHHHHCCCEEEE
LDQEDVRYLLQQSERADYELTVSLEEQRVFDDEGFSRPFDIDPYRKKLLLKGWDEIDLTF
ECHHHHHHHHHHCCCCCEEEEEEECHHEECCCCCCCCCCCCCHHHHHHHHCCCCCEEEEE
VYESHIAAYEQKHCPRP
EEHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA