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The map label for this gene is yoaE [H]

Identifier: 138895239

GI number: 138895239

Start: 1664104

End: 1666143

Strand: Reverse

Name: yoaE [H]

Synonym: GTNG_1579

Alternate gene names: 138895239

Gene position: 1666143-1664104 (Counterclockwise)

Preceding gene: 138895244

Following gene: 138895235

Centisome position: 46.93

GC content: 47.45

Gene sequence:

>2040_bases
ATGGGTTCATTTGTACAACAGCCAACCGGCGTCTTTCCGTCTGTCTGTTCTCTCGATTGTCCTGATCAATGCGGGTTGCT
CGTGCATAAGAAGGATGGGAAAGTCGTAAAAATCCAAGGCGACCCGTCTCATCCTGTGACAAAAGGCCATATATGCAATA
AAGTGCGGAATATGATGGAACGAATCTACGATCCGAAACGTTTGAAATACCCGCTGAAACGTATAGGTGCCAAAGGGGAA
GGGAAATTTGCACGAATCAGCTGGGATGAAGCGCTCGAGACCATTGCGGCCAAATGGAAAGAATTGATTGAAACATACGG
ACCTGAAAGCATCCTCCCTTATAGCTTTTATGGCAACATGGGAAGACTCAGCGCCGAAGGCATGGACCGGCGTTTTTTCC
ATCGGCTAGGCGCATCCTTGCTTGATCGGACCATTTGTTCATCAGCTGGATCTCAAGGGCTTCAATATACGATGGGAGGC
GGCTTTGGAATCGATCCGGAAGAAACCATTCATGCCAAACTCGTCATCTTCTGGGGAATTAATGCGGTCAGCACCAACAT
GCATCAAGTCATCTTAGCCCAAAAAGCCCGAAAAAACGGGGCGAAGATTGTTGTGATTGATGTTCATAAAAACCAAACCG
GACAGCTCGCTGACTGGTTCATTCCGATTCTGCCAGGCACTGATGCCGCTCTCGCTTTAGGCATGATGCATATTTTGTTT
GCGGAAAATCTCATAGATGATGAGTTTTTGAGAAAGTATACGGTCGGCTATGAGGAACTGCGCGAGCACGTTGTCCAATA
TGATCCCGTCACTGTTTCCAACATAACAGGCGTTCCTGTTGAAGATCTTTATACGCTCGCCAGATGGTATGGGCAAACAA
CTCCTTCCTTCATTCGAATCGGCAACGGTTTGCAGCATCACGATAACGGAGGAATGTGCATCCGAACGATCGCTTGTCTC
CCGGCCCTGACCGGACAATGGCTGGTCAAAGGTGGCGGCGCCATCAAATCCAATAGCGGCTACCTAGCTCTTAATCAAAT
AAGCTTGCAGCGCCCGGATCTATTGCAAAACAAACATACACGAATCATCAACATGAATCGGCTTGGTGAGGCATTGTTGG
AATTGGATCCGCCGATTCGTTCGTTATTCGTGTACGGCACAAACCCGGCAGTTGTTGCCCCAAACAGCAACAAAGTACGA
CAAGGATTGGCGAGAGAGGATCTCTTTGTCATCGTGCATGATTTGTTTGTCACAGAAACCGCCAAATACGCCGATATTGT
GCTTCCAGCCACTTCTTCATTTGAAAATACAGATTTGTATACATCCTATTGGCACCACTATGTTCAAATTCAACAACCAG
TGATTGAACGATACGGAGAGTCTAAATCGAATGTCGAAGTGTTCCAATTGCTGGCGAAACGAATGGGGTTTGAGGATCCG
TGTTTATACGAGACAGAAGAAGAAATGATTTCGCAAGCCTTGGATAACCCAACCAACCCTTTCTTGGAAGGAATCCGTTA
TGAAACATTGGTAGAAAAACAGTATATCAAAGCAAAGGTCAAACGGTTGCTGCCGGGAACATTACCAACGCCGAGCGGAA
AAATTGAGCTGTATTCGAAGAAAATGGAACAAGATGGCTATCCTCCGCTGCCAACGTATACGCCGATTGTTGACGATGGA
GATTTCCCGTTTTTCTTTGTGCCCGGTCCTAACCACAACTTTTTAAACACCACGTTCTCCAATAATGAAAAGCACATTTC
GTTGGAAAAAGAACCGCGTTTGTATATGAATGTGAAAGATGCCCTAGCGAAAGGAATTCAAGATGGGGATCGAGTCCGGG
TATGGAACGATCGCGGAGAATGCGTGTTGAAAGCATCGGTTGGAGAACATGTCCTCCCCGGTGTCGTCGTCACGCAAGGG
TTATGGGCTGATTCTCCGGACACGAAACATTTGGTCAACTCCCTTACTCCCGATCGGATCGCCGACATGGGCGGCGGTGC
GACCTTTTTCTCCGGCCGCGTCGATGTGGAAAAATGCTAG

Upstream 100 bases:

>100_bases
AGTTTTATATATCATTGTTGCCATACTCTTCAAATTATTTTATCATATTAACCATCTAACTCAGTCGCCATCATTGATTT
GATCAAGAAAGGAGAAAACG

Downstream 100 bases:

>100_bases
GATGAATGGCTCGAGGGGCTGGCTAGAAATCAATATGTAGGCCAGGCGCCCTCTTTTTTTACCGTTTGTAACTAACGCCA
TCTTTATTCTCCCGTCTCCG

Product: formate dehydrogenase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 679; Mature: 678

Protein sequence:

>679_residues
MGSFVQQPTGVFPSVCSLDCPDQCGLLVHKKDGKVVKIQGDPSHPVTKGHICNKVRNMMERIYDPKRLKYPLKRIGAKGE
GKFARISWDEALETIAAKWKELIETYGPESILPYSFYGNMGRLSAEGMDRRFFHRLGASLLDRTICSSAGSQGLQYTMGG
GFGIDPEETIHAKLVIFWGINAVSTNMHQVILAQKARKNGAKIVVIDVHKNQTGQLADWFIPILPGTDAALALGMMHILF
AENLIDDEFLRKYTVGYEELREHVVQYDPVTVSNITGVPVEDLYTLARWYGQTTPSFIRIGNGLQHHDNGGMCIRTIACL
PALTGQWLVKGGGAIKSNSGYLALNQISLQRPDLLQNKHTRIINMNRLGEALLELDPPIRSLFVYGTNPAVVAPNSNKVR
QGLAREDLFVIVHDLFVTETAKYADIVLPATSSFENTDLYTSYWHHYVQIQQPVIERYGESKSNVEVFQLLAKRMGFEDP
CLYETEEEMISQALDNPTNPFLEGIRYETLVEKQYIKAKVKRLLPGTLPTPSGKIELYSKKMEQDGYPPLPTYTPIVDDG
DFPFFFVPGPNHNFLNTTFSNNEKHISLEKEPRLYMNVKDALAKGIQDGDRVRVWNDRGECVLKASVGEHVLPGVVVTQG
LWADSPDTKHLVNSLTPDRIADMGGGATFFSGRVDVEKC

Sequences:

>Translated_679_residues
MGSFVQQPTGVFPSVCSLDCPDQCGLLVHKKDGKVVKIQGDPSHPVTKGHICNKVRNMMERIYDPKRLKYPLKRIGAKGE
GKFARISWDEALETIAAKWKELIETYGPESILPYSFYGNMGRLSAEGMDRRFFHRLGASLLDRTICSSAGSQGLQYTMGG
GFGIDPEETIHAKLVIFWGINAVSTNMHQVILAQKARKNGAKIVVIDVHKNQTGQLADWFIPILPGTDAALALGMMHILF
AENLIDDEFLRKYTVGYEELREHVVQYDPVTVSNITGVPVEDLYTLARWYGQTTPSFIRIGNGLQHHDNGGMCIRTIACL
PALTGQWLVKGGGAIKSNSGYLALNQISLQRPDLLQNKHTRIINMNRLGEALLELDPPIRSLFVYGTNPAVVAPNSNKVR
QGLAREDLFVIVHDLFVTETAKYADIVLPATSSFENTDLYTSYWHHYVQIQQPVIERYGESKSNVEVFQLLAKRMGFEDP
CLYETEEEMISQALDNPTNPFLEGIRYETLVEKQYIKAKVKRLLPGTLPTPSGKIELYSKKMEQDGYPPLPTYTPIVDDG
DFPFFFVPGPNHNFLNTTFSNNEKHISLEKEPRLYMNVKDALAKGIQDGDRVRVWNDRGECVLKASVGEHVLPGVVVTQG
LWADSPDTKHLVNSLTPDRIADMGGGATFFSGRVDVEKC
>Mature_678_residues
GSFVQQPTGVFPSVCSLDCPDQCGLLVHKKDGKVVKIQGDPSHPVTKGHICNKVRNMMERIYDPKRLKYPLKRIGAKGEG
KFARISWDEALETIAAKWKELIETYGPESILPYSFYGNMGRLSAEGMDRRFFHRLGASLLDRTICSSAGSQGLQYTMGGG
FGIDPEETIHAKLVIFWGINAVSTNMHQVILAQKARKNGAKIVVIDVHKNQTGQLADWFIPILPGTDAALALGMMHILFA
ENLIDDEFLRKYTVGYEELREHVVQYDPVTVSNITGVPVEDLYTLARWYGQTTPSFIRIGNGLQHHDNGGMCIRTIACLP
ALTGQWLVKGGGAIKSNSGYLALNQISLQRPDLLQNKHTRIINMNRLGEALLELDPPIRSLFVYGTNPAVVAPNSNKVRQ
GLAREDLFVIVHDLFVTETAKYADIVLPATSSFENTDLYTSYWHHYVQIQQPVIERYGESKSNVEVFQLLAKRMGFEDPC
LYETEEEMISQALDNPTNPFLEGIRYETLVEKQYIKAKVKRLLPGTLPTPSGKIELYSKKMEQDGYPPLPTYTPIVDDGD
FPFFFVPGPNHNFLNTTFSNNEKHISLEKEPRLYMNVKDALAKGIQDGDRVRVWNDRGECVLKASVGEHVLPGVVVTQGL
WADSPDTKHLVNSLTPDRIADMGGGATFFSGRVDVEKC

Specific function: Terminal Reductase During Anaerobic Growth On Various Sulfoxide And N-Oxide Compounds. Allows E.Coli To Grow Anaerobically On Me(2)So As Respiratory Oxidant. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=763, Percent_Identity=31.4547837483617, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI1787870, Length=766, Percent_Identity=31.331592689295, Blast_Score=272, Evalue=4e-74,
Organism=Escherichia coli, GI171474008, Length=760, Percent_Identity=30.6578947368421, Blast_Score=272, Evalue=5e-74,
Organism=Escherichia coli, GI145693196, Length=721, Percent_Identity=26.2135922330097, Blast_Score=172, Evalue=8e-44,
Organism=Escherichia coli, GI3868721, Length=686, Percent_Identity=24.9271137026239, Blast_Score=159, Evalue=6e-40,
Organism=Escherichia coli, GI1787231, Length=698, Percent_Identity=23.9255014326648, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI87081994, Length=720, Percent_Identity=25.5555555555556, Blast_Score=153, Evalue=4e-38,
Organism=Escherichia coli, GI1788534, Length=541, Percent_Identity=22.9205175600739, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI3868719, Length=412, Percent_Identity=26.6990291262136, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1787477, Length=297, Percent_Identity=22.8956228956229, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1787778, Length=301, Percent_Identity=25.2491694352159, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 75864; Mature: 75733

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: PS00490 MOLYBDOPTERIN_PROK_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSFVQQPTGVFPSVCSLDCPDQCGLLVHKKDGKVVKIQGDPSHPVTKGHICNKVRNMME
CCCCCCCCCCCCCCHHCCCCCCCCCEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHH
RIYDPKRLKYPLKRIGAKGEGKFARISWDEALETIAAKWKELIETYGPESILPYSFYGNM
HHCCCHHHCCHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCC
GRLSAEGMDRRFFHRLGASLLDRTICSSAGSQGLQYTMGGGFGIDPEETIHAKLVIFWGI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEEEEECC
NAVSTNMHQVILAQKARKNGAKIVVIDVHKNQTGQLADWFIPILPGTDAALALGMMHILF
CHHHCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEHEEEEECCCCHHHHHHHHHHHHH
AENLIDDEFLRKYTVGYEELREHVVQYDPVTVSNITGVPVEDLYTLARWYGQTTPSFIRI
HHHCCCHHHHHHHHCCHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCEEE
GNGLQHHDNGGMCIRTIACLPALTGQWLVKGGGAIKSNSGYLALNQISLQRPDLLQNKHT
CCCCEECCCCCHHHHHHHHHHHCCCCEEEECCCEEECCCCEEEEEEECCCCCHHHCCCCC
RIINMNRLGEALLELDPPIRSLFVYGTNPAVVAPNSNKVRQGLAREDLFVIVHDLFVTET
EEEEHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCHHHHHCCCHHHHHHHHHHHHHHCC
AKYADIVLPATSSFENTDLYTSYWHHYVQIQQPVIERYGESKSNVEVFQLLAKRMGFEDP
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCC
CLYETEEEMISQALDNPTNPFLEGIRYETLVEKQYIKAKVKRLLPGTLPTPSGKIELYSK
CCCCCHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHH
KMEQDGYPPLPTYTPIVDDGDFPFFFVPGPNHNFLNTTFSNNEKHISLEKEPRLYMNVKD
HHHCCCCCCCCCCCCEEECCCCCEEEECCCCCCCEECEECCCCCEEEECCCCCEEEEHHH
ALAKGIQDGDRVRVWNDRGECVLKASVGEHVLPGVVVTQGLWADSPDTKHLVNSLTPDRI
HHHHCCCCCCEEEEECCCCCEEEEECCCCCCCCCHHHCCCCCCCCCCHHHHHHCCCHHHH
ADMGGGATFFSGRVDVEKC
HHCCCCCEEECCCEECCCC
>Mature Secondary Structure 
GSFVQQPTGVFPSVCSLDCPDQCGLLVHKKDGKVVKIQGDPSHPVTKGHICNKVRNMME
CCCCCCCCCCCCCHHCCCCCCCCCEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHH
RIYDPKRLKYPLKRIGAKGEGKFARISWDEALETIAAKWKELIETYGPESILPYSFYGNM
HHCCCHHHCCHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCC
GRLSAEGMDRRFFHRLGASLLDRTICSSAGSQGLQYTMGGGFGIDPEETIHAKLVIFWGI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEEEEECC
NAVSTNMHQVILAQKARKNGAKIVVIDVHKNQTGQLADWFIPILPGTDAALALGMMHILF
CHHHCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEHEEEEECCCCHHHHHHHHHHHHH
AENLIDDEFLRKYTVGYEELREHVVQYDPVTVSNITGVPVEDLYTLARWYGQTTPSFIRI
HHHCCCHHHHHHHHCCHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCEEE
GNGLQHHDNGGMCIRTIACLPALTGQWLVKGGGAIKSNSGYLALNQISLQRPDLLQNKHT
CCCCEECCCCCHHHHHHHHHHHCCCCEEEECCCEEECCCCEEEEEEECCCCCHHHCCCCC
RIINMNRLGEALLELDPPIRSLFVYGTNPAVVAPNSNKVRQGLAREDLFVIVHDLFVTET
EEEEHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCHHHHHCCCHHHHHHHHHHHHHHCC
AKYADIVLPATSSFENTDLYTSYWHHYVQIQQPVIERYGESKSNVEVFQLLAKRMGFEDP
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCC
CLYETEEEMISQALDNPTNPFLEGIRYETLVEKQYIKAKVKRLLPGTLPTPSGKIELYSK
CCCCCHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHH
KMEQDGYPPLPTYTPIVDDGDFPFFFVPGPNHNFLNTTFSNNEKHISLEKEPRLYMNVKD
HHHCCCCCCCCCCCCEEECCCCCEEEECCCCCCCEECEECCCCCEEEECCCCCEEEEHHH
ALAKGIQDGDRVRVWNDRGECVLKASVGEHVLPGVVVTQGLWADSPDTKHLVNSLTPDRI
HHHHCCCCCCEEEEECCCCCEEEEECCCCCCCCCHHHCCCCCCCCCCHHHHHHCCCHHHH
ADMGGGATFFSGRVDVEKC
HHCCCCCEEECCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]